 By increasing the growth factors up to 60 ng/mL of "β"-FGF and EGF and up to 30 ng/mL of activin A/"β"-cellulin, the production of insulin producing cells increased.However increasing the concentration of the growth factors further, had no additional effect on the increase.This study can possibly be the insight for developing a new way to treat type-1 diabetes, which currently can only be treated with injection of insulin.