 The Empress contracted a fever during the third month of her seventh pregnancy, which resulted in antenatal complications that caused her to miscarry a stillborn son.Her health further deteriorated due to an infection, and she died two weeks later on 1 May 1539, aged 35.Charles was left so grief-stricken by his wife's death that for two months he shut himself up in a monastery, where he prayed and mourned for her in solitude.