 This suggests that there is greater expression of KISS1 and potentially even GPR54 at the onset of puberty leading to an increase in kisspeptin/GPR54 signaling that results in the activation of the gonadotropin pathway.The addition of kisspeptin to female rats who had yet to mature led to the initiation of gonadotropin pathway.In humans, it was shown that females at the beginning stages of puberty had much higher kisspeptin levels than those females of the same age who had yet to begin puberty.