 His epitaph is given by Wolf and Landshuth.Rabbi Zacuto applied himself with great diligence to the study of the Kabbalah under Ḥayyim Vital's pupil Benjamin ha-Levi, who had come to Italy from Safed; and this remained the chief occupation of his life.He established a seminary for the study of the Kabbalah, and his favorite pupils, Benjamin ha-Kohen and Abraham Rovigo, often visited him for months at a time at Venice or Mantua, to investigate kabalistic mysteries.