 Fractions I, II, and III are precipitated out at earlier stages.The conditions of the earlier stages are 8% ethanol, pH 7.2, −3 °C, and 5.1% protein for Fraction I; 25% ethanol, pH of 6.9, −5 °C, and 3% protein.The albumin remains in the supernatant fraction during the solid/liquid extraction under these conditions.