 In rat hypothalamus, it was found that over three-fourths of GnRH neurons coexpress the receptor for kisspeptin, GPR54, in their RNA.Kisspeptin was also able to bring about the release of GnRH both ex vivo and in vivo in rat and sheep.It can be concluded that by activating GnRH neurons in the hypothalamus, kisspeptin causes GnRH release which leads to the release of FSH and LH.