 The mechanism for channel opening is likely contributed to a combination of calcium concentrations, voltage, and phosphoregulation integration, in order to govern the conduction of ions through TPCs.Two-pore channels were analyzed by using cell biological methods, endolysosomal patch clamp techniques, and a variety of other methods to study their functions.From these, it was suggested that TPCs have some power in controlling the luminal pH in endolysosomal vesicles.