 Seven years later in 1902, Itersonion developed an enrichment culture for "P. stutzeri," which was later described by van Niel and Allen in 1952.The enrichment medium is a mineral medium with 2% nitrate and tartrate (or malate, succinate, malonate, citrate, ethanol, or acetate) grown under anaerobic conditions at 37 °C.The organism has been isolated from a wide variety of places such as human spinal fluid, straw, manure, soil, and canal water.