 However, the role of inter-domain interactions in protein folding and in energetics of stabilisation of the native structure, probably differs for each protein.In T4 lysozyme, the influence of one domain on the other is so strong that the entire molecule is resistant to proteolytic cleavage.In this case, folding is a sequential process where the C-terminal domain is required to fold independently in an early step, and the other domain requires the presence of the folded C-terminal domain for folding and stabilisation.