 Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536).To perform a SGA analysis in "S.cerevisae", the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains).The resulting diploids are then sporulated by transferring to a media containing reduced nitrogen.