 Tissue biopsy is not usually indicated, but if carried out, the histopathologic appearance is usually normal salivary gland tissue.Sometimes attempted biopsy of Stafne defects reveals an empty cavity (possibly because the gland was displaced at the time of biopsy), or other contents such as blood vessels, fat, lymphoid or connective tissues.Defects of the anterior lingual mandible may require biopsy for correct diagnosis at this unusual location.