 The moderate risk level of containment was intended for experiments in which there was a probability of generating an agent with a significant potential for pathogenicity or ecological disruption.High-risk containment was intended for experiments in which the potential for ecological disruption or pathogenicity of the modified organism could be severe and thereby pose a serious biohazard to laboratory personnel or to the public.These levels of containments, along with the previously mentioned safety measures, formed the basis for the guidelines used by investigators in future experiments that involved the construction and propagation of recombinant DNA molecules using DNA from prokaryotes, bacteriophages and other plasmids, animal viruses and eukaryotes.