 Colama (I Congresso Latino-Americano De Metodos Alternativos Ao Uso De Animais No Ensino, Pesquisa E Industria).On January 1, 2013, EU Directive 2010/63/EU ""on the protection of animals used for scientific purposes"" entered into force for the EU Member States (MS), repealing Directive 86/609/EEC.Because it is a Directive, it allows Member States certain flexibility in transposition of national rules.