title: Ford Laser

text: In Taiwan, Ford Lio Ho Motor renamed the KN/KQ Laser sedan as Ford Tierra while the Laser hatchback was renamed Ford Life, and both was sold as rebadged versions of the pre-facelift Mazda 323 BJ generation without any exterior redesigns. The KQ facelift design was directed by Ford Lio Ho Motor and was based on the Australian KN Laser as the rear fenders are different from the Mazda-derived Ford Tierras. A facelift of the Tierra arrived in the early-to-mid 2000s, distinguished by its completely new front fenders, new hood, a new grille with chrome moulding, new headlights, revised tail lights, new trunk lid, different exterior colours, and slightly revised interiors. When the facelift for the Tierra was introduced, the pre-facelift model sold alongside it, renamed as Ford Activa and acting as a cheaper alternative. The Activa was sold in both sedan and hatchback forms and a wider variety of colour choices was also available while the Tierra facelift was only offered in sedan form and was offered in a variety of trim levels. The KQ Tierra was sold originally as the Tierra LS and the Tierra RS with a slight performance tuning and body kit. In 2005, the RS was replaced by the Tierra Aero which was a slightly tuned down version of the RS, and later in 2007, the Tierra GT was introduced and later the Tierra XT with a redesigned body kit before the model was replaced by the second generation European Ford Focus.


title: Ford Laser

text: The fifth generation Laser was marketed in the Philippines and Malaysia as the Lynx. In the Philippines, two trim levels were offered; GSi and Ghia. Both powered with a 1.6-litre petrol engine. Transmission choices were a 5-speed manual or 4-speed automatic, available in both trims. In 2002, the Lynx received its first facelift, featuring new front fenders, hood, a new grille with chrome moulding, new headlights, tail lights, trunk lid, wheel designs, different exterior colours, and slightly revised interiors. The Ghia grade had an electronic sun visor and beige color tone interior. The LSi was added into the lineup as a base model. It was offered with a 1.3-litre engine, no power features, steel wheel with hubcaps and 2-DIN head unit, and was sold until 2004.


title: Ford Laser

text: In mid-2003, a second facelift took place, featuring new taillights, front grille, redesigned trunk garnish and bumpers. A new addition was a trim level called the RS. It had a 2.0-litre engine making 143 hp, RS body kit and interior, smoked black headlights and 7-spoke RS wheels. Available colors were Red Metallic and Black Metallic and the only transmission choice was a 5-speed manual.


title: Ford Laser

text: In Malaysia, only one trim level was originally offered; the 1.6-litre Ghia. It had front discs and rear drum brakes. Transmission choices were a 5-speed manual or 4-speed automatic. In 2002, the Lynx received its first facelift. The RS trim was released in mid-2003 along with a second facelift.


title: Ford Laser

text: The Laser was replaced in most markets around the world by the European-sourced Focus, designated as one of Ford's "world cars". The Mazda 323's replacement, the first generation Mazda3, was also based on the same platform as the Focus, continuing the tradition of both companies having products in this market segment around the world sharing common componentry.


title: Jain University

text: Jain University, officially JAIN (Deemed-to-be University), is a private deemed-to-be-university in Bangalore, India. Originating from Sri Bhagawan Mahaveer Jain College, it was conferred the deemed-to-be-university status in 2009. In August 2019 Jain University opened its off campus in Kochi, Kerala.


title: Jain University

text: Jain University originates from Sri Bhagawan Mahaveer Jain College (SBMJC), established by the founder and chairman of JGI GROUP, Dr.Chenraj Roychand in 1990. It was conferred deemed to be university status in 2009.


title: Jain University

text: Jain (Deemed-to-be University) includes the following colleges and centers, all in Bangalore, unless otherwise noted:


title: Jain University

text: Jain University offers more than 200 UG and PG programs in commerce, sciences, humanities and arts, engineering and technology and management. It also offers courses for working professionals in the field of Business Administration, Engineering and Information technology.


title: Jain University

text: The National Institutional Ranking Framework (NIRF) ranked the institution 79 among universities and 101-150 overall in 2020. It also ranked it 117 among engineering colleges and 86 among business schools. "Outlook India" ranked it 68 among universities.


title: Jain University

text: Jain (Deemed-to-be University) campus housing system can accommodate nearly 85 percent of the student population.


title: Jain University

text: Jain (Deemed-to-be University) offers both on-campus (JGI Global Campus) and off-campus accommodations in different locations in Bangalore. For most of their hostel accommodation they have a contact with a company called Campus Students Communities(CSC). The residential arrangements are separate for the sexes. Jain (Deemed-to-be University) accommodations are managed by the Hostel Management Division.


title: Prince Ernst August of Hanover (born 1983)

text: Ernst August, Hereditary Prince of Hanover (; born 19 July 1983) is the eldest child of Ernst August, Prince of Hanover (head of the ancient House of Welf which once ruled the Kingdom of Hanover), and his former wife Chantal Hochuli. Due to his father's second marriage, he is also the stepson of Caroline, Princess of Hanover, a Monegasque Princess and the sister of Albert II of Monaco.


text: Ernst August and his younger brother Christian were born in Hildesheim, Lower Saxony, while their half-sister, Alexandra, was born in Austria and lives with her mother in Monaco. Ernst August was baptized on 15 October 1983 at Marienburg Castle, his godparents including Felipe VI of Spain and Constantine II of Greece.


text: Despite the family's residing in Germany through both World Wars, his paternal grandfather, as a descendant of Queen Victoria distantly in the British line of succession, obtained British citizenship for himself and his children in November 1956 under the Sophia Naturalization Act 1705. Their family owned property and conducted business in the post-war United Kingdom. Until his mid-teens, Ernst August and his brother lived at Hurlingham Lodge in London.


text: He also descends from Germany's last emperor, Wilhelm II, following whose abdication at the end of World War I the Hanovers also lost sovereignty over the Duchy of Brunswick, while retaining much of their continental personal property.


text: Ernst August attended Malvern College and then studied history and national economy in New York City. After moving back to London he worked in the banking business for some years. Among other jobs, he worked with an open-end fund of the Islamic Investment Bank in Bahrain and with a London investment bank specialized on financing smaller mining and technology companies in newly industrialized countries.


text: Already in 2004, his father had signed over to him the German property of the House of Hanover, including gothic-revival Marienburg Castle, the agricultural estates of Calenberg Castle and the "Fürstenhaus" ("Princely House") at Herrenhausen Gardens in Hanover; the elaborate museum in this small palace, built by King George I of Great Britain in 1720, has been closed to the public since 2011. Since 2004, the prince has taken over many representative tasks on behalf of his father.


text: The father remained in charge of the Austrian family assets until 2013 when he was removed from the chairmanship of a family foundation based in Liechtenstein, the Duke of Cumberland Foundation, which holds the properties near Gmunden in Austria, the Hanovers' main residence in exile after 1866 when their Kingdom of Hanover was annexed by Prussia. Instead, the younger Ernst August was put in charge, reportedly for negligence on part of his father, at the initiative of the foundation's trustee Prince Michael of Liechtenstein. The foundation manages vast forests, "Cumberland Wildlife Park", the Queen's Villa, a hunting lodge and the mausoleum at Cumberland Castle. In 2017 Ernst August the Elder filed legal action to recover chairmanship.


text: In 2014, Ernst August lent a number of paintings and objects for a Lower Saxony state exhibition, "When the Royals came from Hanover - The rulers of Hanover on England's throne", which included exhibits in five museums and castles under the auspices of Charles, Prince of Wales. Thirty of more than 1000 items were contributed by Elizabeth II, including the State Crown of George I, while Ernst August provided the king's famous Augsburg silver throne and other furniture dating to 1720. He hosted a parallel exhibition, "The Way to the Crown", at Marienburg Castle until through 2016, displaying—among other items—the crown jewels of the Kingdom of Hanover.


text: In 2005 he sold large parts of the family's art and furniture collections in an auction at Sotheby's, with a result of 44 million euros. He had initially planned to allocate the funds in a foundation for the renovation and maintenance of Marienburg Castle; however, he was forced to use most of it to settle his father's debt, which, at the time, had been unknown to him, as he told a newspaper in 2019. In 2011 he sold the manor house and stables at Calenberg, retaining the land. Stating that its proceeds wouldn't suffice to maintain Marienburg Castle, infested with dry rot and threatened by static problems, he entered into negotiations with the State of Lower Saxony regarding the future of the castle, and its renovation, estimated at 27 million euros. In November 2018 he announced that he planned to transfer the castle to a state-controlled foundation (the Hanoverian Monasteries' Chamber, founded by his family in 1569, which still owns most of the secularized monasteries and ecclesiastical estates of the former kingdom), at a symbolic sale price of 1 euro, with the foundation undertaking renovation. The remaining art collection was planned to be kept in the castle, with parts purchased by the state, parts kept by the family and lent to the state, and parts transferred to a foundation controlled by both the family and the state. The transaction, however, was stopped by his father's legal action, seeking to regain ownership.


text: In the summer of 2016 Ernst August became engaged to Ekaterina Igorievna Malysheva (b. 30 July 1986, Apatity, Russia), a Russian designer, general manager of Audiotube and founder of EKAT, and daughter of Igor Malyshev and Svetlana Malysheva. Days before the wedding, the elder Ernst August publicly stated concerns about potential adverse impacts on family assets if the younger Ernst August were to marry his chosen fiancée. Despite the dynastic tradition of obtaining the head of House Hanover's express, prior authorization for an heir's marriage in accordance with an 1836 Hanoverian house law (as Ernst August's father had done when marrying his sons' future mother in 1981), the bridegroom's father declared his intention to withhold consent for his son's marriage to Ekaterina Malysheva, reportedly in a dispute over family assets.


text: Nonetheless, the civil marriage took place on 6 July 2017 in Hanover's New Town Hall and was conducted by the mayor of Hanover, Stefan Schostok. The church marriage took place on 8 July 2017 in the Hanover Market Church at which the former "Landesbischof" of the Evangelical-Lutheran Church of Hanover, Horst Hirschler, presided. The bride was escorted by her father, Igor Malyshev. Her wedding dress was the work of Lebanese designer Sandra Mansour, and she wore a tiara that belonged to the former Kaiser Wilhelm II's only daughter, Princess Victoria Louise of Prussia.


text: Among the wedding guests were: Prince Christian of Hanover, Princess Alexandra of Hanover, Andrea Casiraghi, Tatiana Santo Domingo, Pierre Casiraghi, Beatrice Borromeo, Charlotte Casiraghi, Georg Friedrich, Prince of Prussia and his wife Princess Sophie, Crown Prince Pavlos of Greece and his wife Crown Princess Marie-Chantal, Hereditary Prince Hubertus of Saxe-Coburg and Gotha, Alexander, Prince of Schaumburg-Lippe and Princess Elisabeth von Thurn und Taxis. The father of the groom, Prince Ernst August, did not attend the wedding. From the church, the royal couple rode in a horse carriage to Herrenhausen Gardens for the wedding reception in the Gallery Building. In the evening, a ball took place at Marienburg Castle.


text: In Germany, the legal privileges of royalty and nobility were abolished in 1919; thereafter for legal purposes, hereditary titles form part of the surname only. Nonetheless, while descendants of non-dynastic marriages may have a legal right to the use of "Prinz/Prinzessin von Hannover Herzog/Herzogin zu Braunschweig und Lüneburg Königlicher Prinz/Prinzessin von Großbritannien und Irland" as surnames, they are not recognized as bearing rank, styles titles or membership appertaining to the House of Hanover, according to its house rules. Nor is the title "Prince/ss of Great Britain and Ireland", re-claimed by the deposed House of Hanover in 1931, accorded by British sovereigns to members thereof born after 30 November 1917.


title: Biosensor

text: A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector.


title: Biosensor

text: The "sensitive biological element", e.g. tissue, microorganisms, organelles, cell receptors, enzymes, antibodies, nucleic acids, etc., is a biologically derived material or biomimetic component that interacts with, binds with, or recognizes the analyte under study. The biologically sensitive elements can also be created by biological engineering.


title: Biosensor

text: The "transducer" or the "detector element", which transforms one signal into another one, works in a physicochemical way: optical, piezoelectric, electrochemical,


title: Biosensor

text: electrochemiluminescence etc., resulting from the interaction of the analyte with the biological element, to easily measure and quantify.


title: Biosensor

text: The biosensor reader device connects with the associated electronics or signal processors that are primarily responsible for the display of the results in a user-friendly way. This sometimes accounts for the most expensive part of the sensor device, however it is possible to generate a user friendly display that includes transducer and sensitive element (holographic sensor). The readers are usually custom-designed and manufactured to suit the different working principles of biosensors.


title: Biosensor

text: A biosensor typically consists of a bio-receptor (enzyme/antibody/cell/nucleic acid/aptamer), transducer component (semi-conducting material/nanomaterial), and electronic system which includes a signal amplifier, processor & display. Transducers and electronics can be combined, e.g., in CMOS-based microsensor systems. The recognition component, often called a bioreceptor, uses biomolecules from organisms or receptors modeled after biological systems to interact with the analyte of interest. This interaction is measured by the biotransducer which outputs a measurable signal proportional to the presence of the target analyte in the sample. The general aim of the design of a biosensor is to enable quick, convenient testing at the point of concern or care where the sample was procured.


title: Biosensor

text: In a biosensor, the bioreceptor is designed to interact with the specific analyte of interest to produce an effect measurable by the transducer. High selectivity for the analyte among a matrix of other chemical or biological components is a key requirement of the bioreceptor. While the type of biomolecule used can vary widely, biosensors can be classified according to common types of bioreceptor interactions involving: antibody/antigen, enzymes/ligands, nucleic acids/DNA, cellular structures/cells, or biomimetic materials.


title: Biosensor

text: An immunosensor utilizes the very specific binding affinity of antibodies for a specific compound or antigen. The specific nature of the antibody-antigen interaction is analogous to a lock and key fit in that the antigen will only bind to the antibody if it has the correct conformation. Binding events result in a physicochemical change that in combination with a tracer, such as fluorescent molecules, enzymes, or radioisotopes, can generate a signal. There are limitations with using antibodies in sensors: 1. The antibody binding capacity is strongly dependent on assay conditions (e.g. pH and temperature), and 2. the antibody-antigen interaction is generally robust, however, binding can be disrupted by chaotropic reagents, organic solvents, or even ultrasonic radiation.


title: Biosensor

text: Antibody-antigen interactions can also be used for serological testing, or the detection of circulating antibodies in response to a specific disease. Importantly, serology tests have become an important part of the global response to the COVID-19 pandemic.


title: Biosensor

text: The use of antibodies as the bio-recognition component of biosensors has several drawbacks. They have high molecular weights and limited stability, contain essential disulfide bonds and are expensive to produce. In one approach to overcome these limitations, recombinant binding fragments (Fab, Fv or scFv) or domains (VH, VHH) of antibodies have been engineered. In another approach, small protein scaffolds with favorable biophysical properties have been engineered to generate artificial families of Antigen Binding Proteins (AgBP), capable of specific binding to different target proteins while retaining the favorable properties of the parent molecule. The elements of the family that specifically bind to a given target antigen, are often selected in vitro by display techniques: phage display, ribosome display, yeast display or mRNA display. The artificial binding proteins are much smaller than antibodies (usually less than 100 amino-acid residues), have a strong stability, lack disulfide bonds and can be expressed in high yield in reducing cellular environments like the bacterial cytoplasm, contrary to antibodies and their derivatives. They are thus especially suitable to create biosensors.


title: Biosensor

text: The specific binding capabilities and catalytic activity of enzymes make them popular bioreceptors. Analyte recognition is enabled through several possible mechanisms: 1) the enzyme converting the analyte into a product that is sensor-detectable, 2) detecting enzyme inhibition or activation by the analyte, or 3) monitoring modification of enzyme properties resulting from interaction with the analyte. The main reasons for the common use of enzymes in biosensors are: 1) ability to catalyze a large number of reactions; 2) potential to detect a group of analytes (substrates, products, inhibitors, and modulators of the catalytic activity); and 3) suitability with several different transduction methods for detecting the analyte. Notably, since enzymes are not consumed in reactions, the biosensor can easily be used continuously. The catalytic activity of enzymes also allows lower limits of detection compared to common binding techniques. However, the sensor's lifetime is limited by the stability of the enzyme.


title: Biosensor

text: Antibodies have a high binding constant in excess of 10^8 L/mol, which stands for a nearly irreversible association once the antigen-antibody couple has formed. For certain analyte molecules like glucose affinity binding proteins exist that bind their ligand with a high specificity like an antibody, but with a much smaller binding constant on the order of 10^2 to 10^4 L/mol. The association between analyte and receptor then is of reversible nature and next to the couple between both also their free molecules occur in a measurable concentration. In case of glucose, for instance, concanavalin A may function as affinity receptor exhibiting a binding constant of 4x10^2 L/mol. The use of affinity binding receptors for purposes of biosensing has been proposed by Schultz and Sims in 1979 and was subsequently configured into a fluorescent assay for measuring glucose in the relevant physiological range between 4.4 and 6.1 mmol/L. The sensor principle has the advantage that it does not consume the analyte in a chemical reaction as occurs in enzymatic assays.


title: Biosensor

text: Biosensors employing nucleic acid based receptors can be either based on complementary base pairing interactions referred to as genosensors or specific nucleic acid based antibody mimics (aptamers) as aptasensors. In the former, the recognition process is based on the principle of complementary base pairing, adenine:thymine and cytosine:guanine in DNA. If the target nucleic acid sequence is known, complementary sequences can be synthesized, labeled, and then immobilized on the sensor. The hybridization event can be optically detected and presence of target DNA/RNA ascertained. In the latter, aptamers generated against the target recognise it via interplay of specific non-covalent interactions and induced fitting. These aptamers can be labelled with a fluorophore/metal nanoparticles easily for optical detection or may be employed for label-free electrochemical or cantilever based detection platforms for a wide range of target molecules or complex targets like cells and viruses. Additionally, aptamers can be combined with nucleic acid enzymes, such as RNA-cleaving DNAzymes, providing both target recognition and signal generation in a single molecule, which shows potential applications in the development of multiplex biosensors.


title: Biosensor

text: It has been proposed that properly optimized integrated optical resonators can be exploited for detecting epigenetic modifications (e.g. DNA methylation, histone post-translational modifications) in body fluids from patients affected by cancer or other diseases. Photonic biosensors with ultra-sensitivity are nowadays being developed at a research level to easily detect cancerous cells within the patient's urine. Different research projects aim to develop new portable devices that use cheap, environmentally friendly, disposable cartridges that require only simple handling with no need of further processing, washing, or manipulation by expert technicians.


title: Biosensor

text: Organelles form separate compartments inside cells and usually perform functions independently. Different kinds of organelles have various metabolic pathways and contain enzymes to fulfill its function. Commonly used organelles include lysosome, chloroplast and mitochondria. The spatial-temporal distribution pattern of calcium is closely related to ubiquitous signaling pathway. Mitochondria actively participate in the metabolism of calcium ions to control the function and also modulate the calcium related signaling pathways. Experiments have proved that mitochondria have the ability to respond to high calcium concentrations generated in their proximity by opening the calcium channels. In this way, mitochondria can be used to detect the calcium concentration in medium and the detection is very sensitive due to high spatial resolution. Another application of mitochondria is used for detection of water pollution. Detergent compounds' toxicity will damage the cell and subcellular structure including mitochondria. The detergents will cause a swelling effect which could be measured by an absorbance change. Experiment data shows the change rate is proportional to the detergent concentration, providing a high standard for detection accuracy.


title: Biosensor

text: Cells are often used in bioreceptors because they are sensitive to surrounding environment and they can respond to all kinds of stimulants. Cells tend to attach to the surface so they can be easily immobilized. Compared to organelles they remain active for longer period and the reproducibility makes them reusable. They are commonly used to detect global parameter like stress condition, toxicity and organic derivatives. They can also be used to monitor the treatment effect of drugs. One application is to use cells to determine herbicides which are main aquatic contaminant. Microalgae are entrapped on a quartz microfiber and the chlorophyll fluorescence modified by herbicides is collected at the tip of an optical fiber bundle and transmitted to a fluorimeter. The algae are continuously cultured to get optimized measurement. Results show that detection limit of certain herbicide can reach sub-ppb concentration level. Some cells can also be used to monitor the microbial corrosion. Pseudomonas sp. is isolated from corroded material surface and immobilized on acetylcellulose membrane. The respiration activity is determined by measuring oxygen consumption. There is linear relationship between the current generated and the concentration of sulfuric acid. The response time is related to the loading of cells and surrounding environments and can be controlled to no more than 5min.


title: Biosensor

text: Tissues are used for biosensor for the abundance of enzymes existing. Advantages of tissues as biosensors include the following:


title: Biosensor

text: There also exist some disadvantages of tissues, like the lack of specificity due to the interference of other enzymes and longer response time due to the transport barrier.


title: Biosensor

text: Microbial biosensors exploit the response of bacteria to a given substance. For example, arsenic can be detected using the ars operon found in several bacterial taxon.


title: Biosensor

text: An important part of a biosensor is to attach the biological elements (small molecules/protein/cells) to the surface of the sensor (be it metal, polymer, or glass). The simplest way is to functionalize the surface in order to coat it with the biological elements. This can be done by polylysine, aminosilane, epoxysilane, or nitrocellulose in the case of silicon chips/silica glass. Subsequently, the bound biological agent may also be fixed—for example, by layer by layer deposition of alternatively charged polymer coatings.


title: Biosensor

text: Alternatively, three-dimensional lattices (hydrogel/xerogel) can be used to chemically or physically entrap these (whereby chemically entrapped it is meant that the biological element is kept in place by a strong bond, while physically they are kept in place being unable to pass through the pores of the gel matrix). The most commonly used hydrogel is sol-gel, glassy silica generated by polymerization of silicate monomers (added as tetra alkyl orthosilicates, such as TMOS or TEOS) in the presence of the biological elements (along with other stabilizing polymers, such as PEG) in the case of physical entrapment.


title: Biosensor

text: Another group of hydrogels, which set under conditions suitable for cells or protein, are acrylate hydrogel, which polymerizes upon radical initiation. One type of radical initiator is a peroxide radical, typically generated by combining a persulfate with TEMED (Polyacrylamide gel are also commonly used for protein electrophoresis), alternatively light can be used in combination with a photoinitiator, such as DMPA (2,2-dimethoxy-2-phenylacetophenone). Smart materials that mimic the biological components of a sensor can also be classified as biosensors using only the active or catalytic site or analogous configurations of a biomolecule.


title: Biosensor

text: Biosensors can be classified by their biotransducer type. The most common types of biotransducers used in biosensors are:


title: Biosensor

text: Electrochemical biosensors are normally based on enzymatic catalysis of a reaction that produces or consumes electrons (such enzymes are rightly called redox enzymes). The sensor substrate usually contains three electrodes; a reference electrode, a working electrode and a counter electrode. The target analyte is involved in the reaction that takes place on the active electrode surface, and the reaction may cause either electron transfer across the double layer (producing a current) or can contribute to the double layer potential (producing a voltage). We can either measure the current (rate of flow of electrons is now proportional to the analyte concentration) at a fixed potential or the potential can be measured at zero current (this gives a logarithmic response). Note that potential of the working or active electrode is space charge sensitive and this is often used. Further, the label-free and direct electrical detection of small peptides and proteins is possible by their intrinsic charges using biofunctionalized ion-sensitive field-effect transistors.


title: Biosensor

text: Another example, the potentiometric biosensor, (potential produced at zero current) gives a logarithmic response with a high dynamic range. Such biosensors are often made by screen printing the electrode patterns on a plastic substrate, coated with a conducting polymer and then some protein (enzyme or antibody) is attached. They have only two electrodes and are extremely sensitive and robust. They enable the detection of analytes at levels previously only achievable by HPLC and LC/MS and without rigorous sample preparation. All biosensors usually involve minimal sample preparation as the biological sensing component is highly selective for the analyte concerned. The signal is produced by electrochemical and physical changes in the conducting polymer layer due to changes occurring at the surface of the sensor. Such changes can be attributed to ionic strength, pH, hydration and redox reactions, the latter due to the enzyme label turning over a substrate. Field effect transistors, in which the gate region has been modified with an enzyme or antibody, can also detect very low concentrations of various analytes as the binding of the analyte to the gate region of the FET cause a change in the drain-source current.


title: Biosensor

text: Impedance spectroscopy based biosensor development has been gaining traction nowadays and many such devices / developments are found in the academia and industry. One such device, based on a 4-electrode electrochemical cell, using a nanoporous alumina membrane, has been shown to detect low concentrations of human alpha thrombin in presence of high background of serum albumin. Also interdigitated electrodes have been used for impedance biosensors.


title: Biosensor

text: of target biological molecules. By embedding the ion channels in supported or tethered bilayer membranes (t-BLM) attached to a gold electrode, an electrical circuit is created. Capture molecules such as antibodies can be bound to the ion channel so that the binding of the target molecule controls the ion flow through the channel. This results in a measurable change in the electrical conduction which is proportional to the concentration of the target.


title: Biosensor

text: An ion channel switch (ICS) biosensor can be created using gramicidin, a dimeric peptide channel, in a tethered bilayer membrane. One peptide of gramicidin, with attached antibody, is mobile and one is fixed. Breaking the dimer stops the ionic current through the membrane. The magnitude of the change in electrical signal is greatly increased by separating the membrane from the metal surface using a hydrophilic spacer.


title: Biosensor

text: Quantitative detection of an extensive class of target species, including proteins, bacteria, drug and toxins has been demonstrated using different membrane and capture configurations. The European research project Greensense develops a biosensor to perform quantitative screening of drug-of-abuse such as THC, morphine, and cocaine in saliva and urine.


title: Biosensor

text: A reagentless biosensor can monitor a target analyte in a complex biological mixture without additional reagent. Therefore, it can function continuously if immobilized on a solid support. A fluorescent biosensor reacts to the interaction with its target analyte by a change of its fluorescence properties. A Reagentless Fluorescent biosensor (RF biosensor) can be obtained by integrating a biological receptor, which is directed against the target analyte, and a solvatochromic fluorophore, whose emission properties are sensitive to the nature of its local environment, in a single macromolecule. The fluorophore transduces the recognition event into a measurable optical signal. The use of extrinsic fluorophores, whose emission properties differ widely from those of the intrinsic fluorophores of proteins, tryptophan and tyrosine, enables one to immediately detect and quantify the analyte in complex biological mixtures. The integration of the fluorophore must be done in a site where it is sensitive to the binding of the analyte without perturbing the affinity of the receptor.


title: Biosensor

text: Antibodies and artificial families of Antigen Binding Proteins (AgBP) are well suited to provide the recognition module of RF biosensors since they can be directed against any antigen (see the paragraph on bioreceptors). A general approach to integrate a solvatochromic fluorophore in an AgBP when the atomic structure of the complex with its antigen is known, and thus transform it into a RF biosensor, has been described. A residue of the AgBP is identified in the neighborhood of the antigen in their complex. This residue is changed into a cysteine by site-directed mutagenesis. The fluorophore is chemically coupled to the mutant cysteine. When the design is successful, the coupled fluorophore does not prevent the binding of the antigen, this binding shields the fluorophore from the solvent, and it can be detected by a change of fluorescence. This strategy is also valid for antibody fragments.


title: Biosensor

text: However, in the absence of specific structural data, other strategies must be applied. Antibodies and artificial families of AgBPs are constituted by a set of hypervariable (or randomized) residue positions, located in a unique sub-region of the protein, and supported by a constant polypeptide scaffold. The residues that form the binding site for a given antigen, are selected among the hypervariable residues. It is possible to transform any AgBP of these families into a RF biosensor, specific of the target antigen, simply by coupling a solvatochromic fluorophore to one of the hypervariable residues that have little or no importance for the interaction with the antigen, after changing this residue into cysteine by mutagenesis. More specifically, the strategy consists in individually changing the residues of the hypervariable positions into cysteine at the genetic level, in chemically coupling a solvatochromic fluorophore with the mutant cysteine, and then in keeping the resulting conjugates that have the highest sensitivity (a parameter that involves both affinity and variation of fluorescence signal). This approach is also valid for families of antibody fragments.


title: Biosensor

text: A posteriori studies have shown that the best reagentless fluorescent biosensors are obtained when the fluorophore does not make non-covalent interactions with the surface of the bioreceptor, which would increase the background signal, and when it interacts with a binding pocket at the surface of the target antigen. The RF biosensors that are obtained by the above methods, can function and detect target analytes inside living cells.


title: Biosensor

text: Magnetic biosensors utilize paramagnetic or supra-paramagnetic particles, or crystals, to detect biological interactions. Examples could be coil-inductance, resistance, or other magnetic properties. It is common to use magnetic nano or microparticles. In the surface of such particles are the bioreceptors, that can be DNA (complementary to a sequence or aptamers) antibodies, or others. The binding of the bioreceptor will affect some of the magnetic particle properties that can be measured by AC susceptometry, a Hall Effect sensor, a giant magnetoresistance device, or others.


title: Biosensor

text: Piezoelectric sensors utilise crystals which undergo an elastic deformation when an electrical potential is applied to them. An alternating potential (A.C.) produces a standing wave in the crystal at a characteristic frequency. This frequency is highly dependent on the elastic properties of the crystal, such that if a crystal is coated with a biological recognition element the binding of a (large) target analyte to a receptor will produce a change in the resonance frequency, which gives a binding signal. In a mode that uses surface acoustic waves (SAW), the sensitivity is greatly increased. This is a specialised application of the quartz crystal microbalance as a biosensor


title: Biosensor

text: Electrochemiluminescence (ECL) is nowadays a leading technique in biosensors. Since the excited species are produced with an electrochemical stimulus rather than with a light excitation source, ECL displays improved signal-to-noise ratio compared to photoluminescence, with minimized effects due to light scattering and luminescence background. In particular, coreactant ECL operating in buffered aqueous solution in the region of positive potentials (oxidative-reduction mechanism) definitively boosted ECL for immunoassay, as confirmed by many research applications and, even more, by the presence of important companies which developed commercial hardware for high throughput immunoassays analysis in a market worth billions of dollars each year.


title: Biosensor

text: The MOSFET (metal-oxide-semiconductor field-effect transistor, or MOS transistor) was invented by Mohamed M. Atalla and Dawon Kahng in 1959, and demonstrated in 1960. Two years later, Leland C. Clark and Champ Lyons invented the first biosensor in 1962. Biosensor MOSFETs (BioFETs) were later developed, and they have since been widely used to measure physical, chemical, biological and environmental parameters.


title: Biosensor

text: The first BioFET was the ion-sensitive field-effect transistor (ISFET), invented by Piet Bergveld for electrochemical and biological applications in 1970. the adsorption FET (ADFET) was patented by P.F. Cox in 1974, and a hydrogen-sensitive MOSFET was demonstrated by I. Lundstrom, M.S. Shivaraman, C.S. Svenson and L. Lundkvist in 1975. The ISFET is a special type of MOSFET with a gate at a certain distance, and where the metal gate is replaced by an ion-sensitive membrane, electrolyte solution and reference electrode. The ISFET is widely used in biomedical applications, such as the detection of DNA hybridization, biomarker detection from blood, antibody detection, glucose measurement, pH sensing, and genetic technology.


title: Biosensor

text: By the mid-1980s, other BioFETs had been developed, including the gas sensor FET (GASFET), pressure sensor FET (PRESSFET), chemical field-effect transistor (ChemFET), reference ISFET (REFET), enzyme-modified FET (ENFET) and immunologically modified FET (IMFET). By the early 2000s, BioFETs such as the DNA field-effect transistor (DNAFET), gene-modified FET (GenFET) and cell-potential BioFET (CPFET) had been developed.


title: Biosensor

text: The appropriate placement of biosensors depends on their field of application, which may roughly be divided into biotechnology, agriculture, food technology and biomedicine.


title: Biosensor

text: In biotechnology, analysis of the chemical composition of cultivation broth can be conducted in-line, on-line, at-line and off-line. As outlined by the US Food and Drug Administration (FDA) the sample is not removed from the process stream for in-line sensors, while it is diverted from the manufacturing process for on-line measurements. For at-line sensors the sample may be removed and analyzed in close proximity to the process stream. An example of the latter is the monitoring of lactose in a dairy processing plant. Off-line biosensors compare to bioanalytical techniques that are not operating in the field, but in the laboratory. These techniques are mainly used in agriculture, food technology and biomedicine.


title: Biosensor

text: In medical applications biosensors are generally categorized as "in vitro" and "in vivo" systems. An "in vitro", biosensor measurement takes place in a test tube, a culture dish, a microtiter plate or elsewhere outside a living organism. The sensor uses a bioreceptor and transducer as outlined above. An example of an "in vitro" biosensor is an enzyme-conductimetric biosensor for blood glucose monitoring. There is a challenge to create a biosensor that operates by the principle of point-of-care testing, i.e. at the location where the test is needed. Development of wearable biosensors is among such studies. The elimination of lab testing can save time and money. An application of a POCT biosensor can be for the testing of HIV in areas where it is difficult for patients to be tested. A biosensor can be sent directly to the location and a quick and easy test can be used.


title: Biosensor

text: An "in vivo" biosensor is an implantable device that operates inside the body. Of course, biosensor implants have to fulfill the strict regulations on sterilization in order to avoid an initial inflammatory response after implantation. The second concern relates to the long-term biocompatibility, i.e. the unharmful interaction with the body environment during the intended period of use. Another issue that arises is failure. If there is failure, the device must be removed and replaced, causing additional surgery. An example for application of an in vivo biosensor would be the insulin monitoring within the body, which is not available yet.


title: Biosensor

text: Most advanced biosensor implants have been developed for the continuous monitoring of glucose. The figure displays a device, for which a Ti casing and a battery as established for cardiovascular implants like pacemakers and defibrillators is used. Its size is determined by the battery as required for a lifetime of one year. Measured glucose data will be transmitted wirelessly out of the body within the MICS 402-405 MHz band as approved for medical implants.


title: Biosensor

text: Biosensors can also be integrated into mobile phone systems, making them user-friendly and accessible to a large number of users.


title: Biosensor

text: There are many potential applications of biosensors of various types. The main requirements for a biosensor approach to be valuable in terms of research and commercial applications are the identification of a target molecule, availability of a suitable biological recognition element, and the potential for disposable portable detection systems to be preferred to sensitive laboratory-based techniques in some situations. Some examples are glucose monitoring in diabetes patients, other medical health related targets, environmental applications, e.g. the detection of pesticides and river water contaminants, such as heavy metal ions, remote sensing of airborne bacteria, e.g. in counter-bioterrorist activities, remote sensing of water quality in coastal waters by describing online different aspects of clam ethology (biological rhythms, growth rates, spawning or death records) in groups of abandoned bivalves around the world, detection of pathogens, determining levels of toxic substances before and after bioremediation, detection and determining of organophosphate, routine analytical measurement of folic acid, biotin, vitamin B12 and pantothenic acid as an alternative to microbiological assay, determination of drug residues in food, such as antibiotics and growth promoters, particularly meat and honey, drug discovery and evaluation of biological activity of new compounds, protein engineering in biosensors, and detection of toxic metabolites such as mycotoxins.


title: Biosensor

text: A common example of a commercial biosensor is the blood glucose biosensor, which uses the enzyme glucose oxidase to break blood glucose down. In doing so it first oxidizes glucose and uses two electrons to reduce the FAD (a component of the enzyme) to FADH2. This in turn is oxidized by the electrode in a number of steps. The resulting current is a measure of the concentration of glucose. In this case, the electrode is the transducer and the enzyme is the biologically active component.


title: Biosensor

text: A canary in a cage, as used by miners to warn of gas, could be considered a biosensor. Many of today's biosensor applications are similar, in that they use organisms which respond to toxic substances at a much lower concentrations than humans can detect to warn of their presence. Such devices can be used in environmental monitoring, trace gas detection and in water treatment facilities.


title: Biosensor

text: Commercially available glucose monitors rely on amperometric sensing of glucose by means of glucose oxidase, which oxidises glucose producing hydrogen peroxide which is detected by the electrode. To overcome the limitation of amperometric sensors, a flurry of research is present into novel sensing methods, such as fluorescent glucose biosensors.


title: Biosensor

text: The interferometric reflectance imaging sensor (IRIS) is based on the principles of optical interference and consists of a silicon-silicon oxide substrate, standard optics, and low-powered coherent LEDs. When light is illuminated through a low magnification objective onto the layered silicon-silicon oxide substrate, an interferometric signature is produced. As biomass, which has a similar index of refraction as silicon oxide, accumulates on the substrate surface, a change in the interferometric signature occurs and the change can be correlated to a quantifiable mass. "Daaboul et al." used IRIS to yield a label-free sensitivity of approximately 19 ng/mL. "Ahn et al." improved the sensitivity of IRIS through a mass tagging technique.


title: Biosensor

text: Since initial publication, IRIS has been adapted to perform various functions. First, IRIS integrated a fluorescence imaging capability into the interferometric imaging instrument as a potential way to address fluorescence protein microarray variability. Briefly, the variation in fluorescence microarrays mainly derives from inconsistent protein immobilization on surfaces and may cause misdiagnoses in allergy microarrays. To correct for any variation in protein immobilization, data acquired in the fluorescence modality is then normalized by the data acquired in the label-free modality. IRIS has also been adapted to perform single nanoparticle counting by simply switching the low magnification objective used for label-free biomass quantification to a higher objective magnification. This modality enables size discrimination in complex human biological samples. "Monroe et al." used IRIS to quantify protein levels spiked into human whole blood and serum and determined allergen sensitization in characterized human blood samples using zero sample processing. Other practical uses of this device include virus and pathogen detection.


title: Biosensor

text: There are several applications of biosensors in food analysis. In the food industry, optics coated with antibodies are commonly used to detect pathogens and food toxins. Commonly, the light system in these biosensors is fluorescence, since this type of optical measurement can greatly amplify the signal.


title: Biosensor

text: A range of immuno- and ligand-binding assays for the detection and measurement of small molecules such as water-soluble vitamins and chemical contaminants (drug residues) such as sulfonamides and Beta-agonists have been developed for use on SPR based sensor systems, often adapted from existing ELISA or other immunological assay. These are in widespread use across the food industry.


title: Biosensor

text: Biosensors could be used to monitor air, water, and soil pollutants such as pesticides, potentially carcinogenic, mutagenic, and/or toxic substances and endocrine disrupting chemicals.


title: Biosensor

text: For example, bionanotechnologists developed a viable biosensor, , that can detect levels of diverse water pollutants.


title: Biosensor

text: Because ozone filters out harmful ultraviolet radiation, the discovery of holes in the ozone layer of the earth's atmosphere has raised concern about how much ultraviolet light reaches the earth's surface. Of particular concern are the questions of how deeply into sea water ultraviolet radiation penetrates and how it affects marine organisms, especially plankton (floating microorganisms) and viruses that attack plankton. Plankton form the base of the marine food chains and are believed to affect our planet's temperature and weather by uptake of CO for photosynthesis.


title: Biosensor

text: Deneb Karentz, a researcher at the Laboratory of Radio-biology and Environmental Health (University of California, San Francisco) has devised a simple method for measuring ultraviolet penetration and intensity. Working in the Antarctic Ocean, she submerged to various depths thin plastic bags containing special strains of "E. coli" that are almost totally unable to repair ultraviolet radiation damage to their DNA. Bacterial death rates in these bags were compared with rates in unexposed control bags of the same organism. The bacterial "biosensors" revealed constant significant ultraviolet damage at depths of 10 m and frequently at 20 and 30 m. Karentz plans additional studies of how ultraviolet may affect seasonal plankton blooms (growth spurts) in the oceans.


title: Biosensor

text: Metastasis is the spread of cancer from one part of the body to another via either the circulatory system or lymphatic system. Unlike radiology imaging tests (mammograms), which send forms of energy (x-rays, magnetic fields, etc.) through the body to only take interior pictures, biosensors have the potential to directly test the malignant power of the tumor. The combination of a biological and detector element allows for a small sample requirement, a compact design, rapid signals, rapid detection, high selectivity and high sensitivity for the analyte being studied. Compared to the usual radiology imaging tests biosensors have the advantage of not only finding out how far cancer has spread and checking if treatment is effective but also are cheaper, more efficient (in time, cost and productivity) ways to assess metastaticity in early stages of cancer.


title: Biosensor

text: Biological engineering researchers have created oncological biosensors for breast cancer. Breast cancer is the leading common cancer among women worldwide. An example would be a transferrin- quartz crystal microbalance (QCM). As a biosensor, quartz crystal microbalances produce oscillations in the frequency of the crystal's standing wave from an alternating potential to detect nano-gram mass changes. These biosensors are specifically designed to interact and have high selectivity for receptors on cell (cancerous and normal) surfaces. Ideally, this provides a quantitative detection of cells with this receptor per surface area instead of a qualitative picture detection given by mammograms.


title: Biosensor

text: Seda Atay, a biotechnology researcher at Hacettepe University, experimentally observed this specificity and selectivity between a QCM and MDA-MB 231 breast cells, MCF 7 cells, and starved MDA-MB 231 cells in vitro. With other researchers she devised a method of washing these different metastatic leveled cells over the sensors to measure mass shifts due to different quantities of transferrin receptors. Particularly, the metastatic power of breast cancer cells can be determined by Quartz crystal microbalances with nanoparticles and transferrin that would potentially attach to transferrin receptors on cancer cell surfaces. There is very high selectivity for transferrin receptors because they are over-expressed in cancer cells. If cells have high expression of transferrin receptors, which shows their high metastatic power, they have higher affinity and bind more to the QCM that measures the increase in mass. Depending on the magnitude of the nano-gram mass change, the metastatic power can be determined.


title: Biosensor

text: Additionally, in the last years, significant attentions have been focused to detect the biomarkers of lung cancer without biopsy. In this regard, biosensors are very attractive and applicable tools for providing rapid, sensitive, specific, stable, cost-effective and non-invasive detections for early lung cancer diagnosis. Thus, cancer biosensors consisting of specific biorecognition molecules such as antibodies, complementary nucleic acid probes or other immobilized biomolecules on a transducer surface. The biorecognition molecules interact specifically with the biomarkers (targets) and the generated biological responses are converted by the transducer into a measurable analytical signal. Depending on the type of biological response, various transducers are utilized in the fabrication of cancer biosensors such as electrochemical, optical and mass-based transducers.


title: Biosensor

text: Biosensors could be used for the detection of pathogenic organisms.<ref name="10.1002/bab.1621"></ref>


title: Biosensor

text: Embedded biosensors for pathogenic signatures – such as of SARS-CoV-2 – that are wearable have been developed – such as face masks with built-in tests. See also: COVID-19 public transport R&D


title: Biosensor

text: New types of biosensor-chips could enable novel methods "such as drone-deployed pathogen sensors actively surveying air or wastewater". Protein-binding aptamers could be used for testing for infectious disease pathogens. Systems of electronic skins (or robot skins) with built-in biosensors (or chemical sensors) and human-machine interfaces may enable wearable as well as remote sensed device- or robotic-sensing of pathogens (as well as of several hazardous materials and tactile perceptions).


title: Biosensor

text: Many optical biosensors are based on the phenomenon of surface plasmon resonance (SPR) techniques. This utilises a property of and other materials; specifically that a thin layer of gold on a high refractive index glass surface can absorb laser light, producing electron waves (surface plasmons) on the gold surface. This occurs only at a specific angle and wavelength of incident light and is highly dependent on the surface of the gold, such that binding of a target analyte to a receptor on the gold surface produces a measurable signal.


title: Biosensor

text: Surface plasmon resonance sensors operate using a sensor chip consisting of a plastic cassette supporting a glass plate, one side of which is coated with a microscopic layer of gold. This side contacts the optical detection apparatus of the instrument. The opposite side is then contacted with a microfluidic flow system. The contact with the flow system creates channels across which reagents can be passed in solution. This side of the glass sensor chip can be modified in a number of ways, to allow easy attachment of molecules of interest. Normally it is coated in carboxymethyl dextran or similar compound.


title: Biosensor

text: The refractive index at the flow side of the chip surface has a direct influence on the behavior of the light reflected off the gold side. Binding to the flow side of the chip has an effect on the refractive index and in this way biological interactions can be measured to a high degree of sensitivity with some sort of energy. The refractive index of the medium near the surface changes when biomolecules attach to the surface, and the SPR angle varies as a function of this change.


title: Biosensor

text: Light of a fixed wavelength is reflected off the gold side of the chip at the angle of total internal reflection, and detected inside the instrument. The angle of incident light is varied in order to match the evanescent wave propagation rate with the propagation rate of the surface plasmon polaritons. This induces the evanescent wave to penetrate through the glass plate and some distance into the liquid flowing over the surface.


title: Biosensor

text: Other optical biosensors are mainly based on changes in absorbance or fluorescence of an appropriate indicator compound and do not need a total internal reflection geometry. For example, a fully operational prototype device detecting casein in milk has been fabricated. The device is based on detecting changes in absorption of a gold layer. A widely used research tool, the micro-array, can also be considered a biosensor.


title: Biosensor

text: Biological biosensors, also known as optogenetic sensors, often incorporate a genetically modified form of a native protein or enzyme. The protein is configured to detect a specific analyte and the ensuing signal is read by a detection instrument such as a fluorometer or luminometer. An example of a recently developed biosensor is one for detecting cytosolic concentration of the analyte cAMP (cyclic adenosine monophosphate), a second messenger involved in cellular signaling triggered by ligands interacting with receptors on the cell membrane. Similar systems have been created to study cellular responses to native ligands or xenobiotics (toxins or small molecule inhibitors). Such "assays" are commonly used in drug discovery development by pharmaceutical and biotechnology companies. Most cAMP assays in current use require lysis of the cells prior to measurement of cAMP. A live-cell biosensor for cAMP can be used in non-lysed cells with the additional advantage of multiple reads to study the kinetics of receptor response.


title: Biosensor

text: Nanobiosensors use an immobilized bioreceptor probe that is selective for target analyte molecules. Nanomaterials are exquisitely sensitive chemical and biological sensors. Nanoscale materials demonstrate unique properties. Their large surface area to volume ratio can achieve rapid and low cost reactions, using a variety of designs.


title: Biosensor

text: Other evanescent wave biosensors have been commercialised using waveguides where the propagation constant through the waveguide is changed by the absorption of molecules to the waveguide surface. One such example, dual polarisation interferometry uses a buried waveguide as a reference against which the change in propagation constant is measured. Other configurations such as the Mach–Zehnder have reference arms lithographically defined on a substrate. Higher levels of integration can be achieved using resonator geometries where the resonant frequency of a ring resonator changes when molecules are absorbed.


title: Biosensor

text: Recently, arrays of many different detector molecules have been applied in so called electronic nose devices, where the pattern of response from the detectors is used to fingerprint a substance. In the Wasp Hound odor-detector, the mechanical element is a video camera and the biological element is five parasitic wasps who have been conditioned to swarm in response to the presence of a specific chemical. Current commercial electronic noses, however, do not use biological elements.


title: Biosensor

text: DNA can be the analyte of a biosensor, being detected through specific means, but it can also be used as part of a biosensor or, theoretically, even as a whole biosensor.


title: Biosensor

text: Many techniques exist to detect DNA, which is usually a means to detect organisms that have that particular DNA. DNA sequences can also be used as described above. But more forward-looking approaches exist, where DNA can be synthesized to hold enzymes in a biological, stable gel. Other applications are the design of aptamers, sequences of DNA that have a specific shape to bind a desired molecule. The most innovative processes use DNA origami for this, creating sequences that fold in a predictable structure that is useful for detection.


title: Biosensor

text: Scientists have built prototype sensors to detect DNA of animals from sucked in air, "airborne eDNA".


title: Biosensor

text: "Nanoantennas" made out of DNA – a novel type of nano-scale optical antenna – can be attached to proteins and produce a signal via fluorescence when these perform their biological functions, in particular for distinct conformational changes.


title: Biosensor

text: Graphene is a two-dimensional carbon-based substance with superior optical, electrical, mechanical, thermal, and mechanical properties. The ability to absorb and immobilize a variety of proteins, particularly some with carbon ring structures, has proven graphene to be an excellent candidate as a biosensor transducer. As a result, various graphene-based biosensors have been explored and developed in recent times.


title: Dublin, California

text: Dublin (formerly, Amador and Dougherty's Station) is a suburban city of the East Bay in California. It is located within the Amador Valley of Alameda County's Tri-Valley region. It is located along the north side of Interstate 580 at the intersection with Interstate 680, roughly east of downtown San Francisco, east of downtown Oakland, and north of downtown San Jose.


title: Dublin, California

text: It was referred to as "Dublin" in reference to the city of Dublin, Ireland, because of the large number of Irish who lived there. The post office formally adopted the name in the 1890s.


title: Dublin, California

text: The population was 72,589 as of the 2020 census, which had grown from 46,063 in 2010. In 2013, Dublin was the second fastest-growing city in the state of California, behind only Santa Clarita. In 2019, census data showed Dublin as one of the fastest-growing cities in the country. Dublin was formerly home to the headquarters of Sybase, Inc (now part of SAP SE) and is currently home to the headquarters for CallidusCloud, Patelco Credit Union, Ross Stores, TriNet, Medley Health, Challenge Dairy and Arlen Ness.


title: Dublin, California

text: In 1835, José María Amador was granted 16,500 acres for his service as a Mexican soldier and in Mission San Jose where he was an administrator in the valley which was named Amador Valley after him. In 1850, Irish settlers bought land from Amador and founded a town.


title: Dublin, California

text: Several historical sites are preserved and located where Dublin Boulevard is crossed by Donlon Way, itself formerly the northernmost segment of the main road to Sunol and Niles Canyon (present-day Foothill Road):


title: Dublin, California

text: Dublin Boulevard, a generally east–west road running just north of Interstate 580, was a part of the Lincoln Highway and later U.S. Route 50. The street formerly curved southward near today's Hansen Drive to follow present-day Dublin Canyon Road toward Hayward.


title: Dublin, California

text: In 1960, the first housing tracts were built in West Dublin, transforming the formerly rural community into a suburb. It grew steadily from the early 1960s onward as both a residential and retail center. The City became incorporated in February 1982.


title: Dublin, California

text: Although a post office operated from 1860 to 1908 in Dougherty, which broke off from Dublin, Dublin's first post office was opened in 1963, and is still in operation today.


title: Dublin, California

text: On December 6, 2011, during shooting for an episode of "MythBusters" at Camp Parks, a cannonball went skyward and zoomed through a nearby residence, coming to rest in a car. Many residents were shaken and the community was shocked, but nobody was injured. "MythBusters" cast members Adam Savage and Kari Byron hosted a student engineering event at Dublin High School, a move to express gratitude to the community of Dublin for the access to Camp Parks as a shooting location. The Dublin Film Commission declared December 6 "Victory in the battle for Dublin", poking at the rogue cannonball incident, and announced plans to host a film festival every December 6 to honor Dublin's fallen cannonball. On December 6, 2012, Tri-Valley Film Coalition President Morgan Finley King hosted a film festival at the Dublin Library, in which winners of each category were awarded a "Golden Cannonball Award".


title: Dublin, California

text: In 2000, following a conflict with Mayor Guy Houston and developers of the West Dublin Hills, Morgan King and David Bewley began a ballot initiative known as Measure M. The objective of the measure was to prevent the Dublin Hills from becoming overwhelmed with housing that had been promised to voters and that the housing would not be built on preserved open space. Measure M won in every precinct in Dublin and on the absentee ballots.


title: Dublin, California

text: Wallis Ranch is a subdivision developed by Trumark Communities with approximately 816 units which opened its first phase in September 2016. Trumark divided the project into eight neighborhoods, selling seven of them to other homebuilders including Warmington Residential, PulteGroup, Taylor Morrison, KB Home and D.R. Horton. Architects KTGY Architecture + Planning, Dahlin Group and Gates & Associates were the master plan architects. Teichert Construction built the infrastructure.


title: Dublin, California

text: Tassajara Hills is another subdivision built by Toll Brothers, approximately 370 detached homes. Transit-oriented development around the city's two BART stations continues with the Aster and Valor Crossing developments. Valor Crossing is an affordable housing project specifically designed for veterans and their families. Avalon Dublin Station is another recently completed luxury rental development by AvalonBay Communities.


title: Dublin, California

text: The 2010 United States Census reported that Dublin had a population of 46,036, which grew over 57,000 as of 2016, and nearly 65,000 in 2018. It has been one of the fastest-growing cities in California, with its population nearly doubling during the past decade. Once the building of homes is finished in East Dublin, the city will have a capacity for over 75,000 citizens. The population density in 2010 was 3,087.1 people per square mile (1,192.0/km). The racial makeup of Dublin was 23,634 (51.3%) White, 4,347 (9.4%) African American, 246 (0.5%) Native American, 12,321 (26.8%) Asian, 287 (0.6%) Pacific Islander, 2,458 (5.3%) from other races, and 2,743 (6.0%) from two or more races. Hispanic or Latino of any race were 6,663 persons (14.5%).


title: Dublin, California

text: The Census reported that 40,262 people (87.5% of the population) lived in households, 92 (0.2%) lived in non-institutionalized group quarters, and 5,682 (12.3%) were institutionalized.


title: Dublin, California

text: There were 14,913 households, out of which 5,897 (39.5%) had children under the age of 18 living in them, 8,615 (57.8%) were opposite-sex married couples living together, 1,383 (9.3%) had a female householder with no husband present, 615 (4.1%) had a male householder with no wife present. There were 775 (5.2%) unmarried opposite-sex partnerships, and 142 (1.0%) same-sex married couples or partnerships. 3,201 households (21.5%) were made up of individuals, and 578 (3.9%) had someone living alone who was 65 years of age or older. The average household size was 2.70. There were 10,613 families (71.2% of all households); the average family size was 3.19.


title: Dublin, California

text: The population was spread out, with 10,297 people (22.4%) under the age of 18, 3,703 people (8.0%) aged 18 to 24, 17,587 people (38.2%) aged 25 to 44, 11,092 people (24.1%) aged 45 to 64, and 3,357 people (7.3%) who were 65 years of age or older. The median age was 35.3 years. For every 100 females, there were 108.8 males. For every 100 females age 18 and over, there were 109.4 males.


title: Dublin, California

text: There were 15,782 housing units at an average density of , of which 14,913 were occupied, of which 9,425 (63.2%) were owner-occupied, and 5,488 (36.8%) were occupied by renters. The homeowner vacancy rate was 2.5%; the rental vacancy rate was 5.0%. 26,954 people (58.5% of the population) lived in owner-occupied housing units and 13,308 people (28.9%) lived in rental housing units.


title: Dublin, California

text: The median income for a household in the city was $114,699, and the median income for a family was $128,737. The per capita income for the city was $44,679.


title: Dublin, California

text: In 2000, there were 9,325 households and 6,508 families residing in the city. The population density was . There are about 9,872 housing units at an average density of .


title: Dublin, California

text: There were 9,325 households, out of which 35.4% had children under the age of 18 living with them, 56.9% were married couples living together, 9.1% had a female householder with no husband present, and 30.2% were non-families. 21.3% of all households were made up of individuals, and 2.8% had someone living alone who was 65  years of age or older. The average household size was 2.65 and the average family size was 3.13.


title: Dublin, California

text: In the city, the population was 21.0% under the age of 18, 9.3% from 18 to 24, 44.1% from 25 to 44, 21.0% from 45 to 64, and 4.6% who were 65 years of age or older. The median age was 34 years. For every 100 females, there were 111.2 males. For every 100 females age 18 and over, there were 112.4 males. The average income for a household in the city is $101,550. Males had a median income of $77,605 versus $48,116 for females. The per capita income for the city was $29,451. About 1.9% of families and 2.9% of the population were below the poverty line, including 3.2% of those under age 18 and 3.2% of those age 65 or over.


title: Dublin, California

text: Whole Foods Market, Nordstrom Rack, Dick's Sporting Goods, HomeGoods, and a second Target location, have all opened for business in the Eastern side of the city, along with restaurant chains BJ's Restaurant & Brewhouse, and Lazy Dog Restaurant & Bar.


title: Dublin, California

text: Dublin has also undergone significant remodeling and expansion, with almost every shopping center in Dublin being remodeled while new shopping centers continued to be built. Dublin Place and Dublin Retail Center were followed by Hacienda Crossings, The Shops at Waterford, The Shops at Tralee Village, Grafton Station, Fallon Gateway, and Persimmon Place.


title: Dublin, California

text: Dublin is the site of Santa Rita Jail, the principal jail for Alameda County. The third largest jail in California and the fifth largest in the United States, Santa Rita Jail is considered a "mega-jail", specified to hold 4,000 prisoners at any one time, making it as large as, or larger than, many of California's state prisons. It was completed at a cost of $172 million in 1983. Dublin also hosts the Federal Correctional Institution, Dublin, one of five federal prisons for women in the United States. Designed to house 250 inmates, FCI Dublin currently houses 1,077 as of April 11, 2013. There are 3 areas at FCI Dublin: Low security, Minimum security and the Federal Detention Center.


title: Dublin, California

text: The Parks Reserve Forces Training Area (PRFTA), historically known as Camp Parks, is located in Dublin. A sub-installation of Fort Hunter Liggett, Camp Parks is the only training facility within a short drive for the 11,000-plus reservists in the San Francisco Bay Area. Firing ranges and a wide variety of training facilities are available. The post is home to the Regional Training Site-Intelligence, Regional Training Site-Medical and the 91st DIV Battle Projection Center. Growth is on the horizon as new facilities have been built and more are programmed for construction in the near future.


title: Dublin, California

text: According to the city's 2020 Comprehensive Annual Financial Report, the top employers in the city are:


title: Dublin, California

text: The annual Saint Patrick's Day celebration includes a 5K Fun Run and Walk, a pancake breakfast, a two-day festival, and a parade. The parade is popular with residents and visitors from outside Dublin alike and has been growing in popularity each year. It is sponsored by the Dublin Host Lions Club and features bands and colorful floats. The Dublin firefighters sponsor the pancake breakfast, and tours of the firehouse are popular with children. The festival continues all weekend and features food, games, kiddie rides, arts & crafts, and information about local organizations.


title: Dublin, California

text: The festival had been held near the end of the parade route in Shamrock Village on Amador Valley Blvd but was relocated to the Civic Center on Dublin Blvd in 2007, moving it closer to the growing population in the eastern part of Dublin.


title: Dublin, California

text: Dublin has two dog parks: Dougherty Hills Dog Park, which comprises an area for large dogs and another area for small dogs and a dog run at Bray Commons.


title: Dublin, California

text: Dublin opened Fallon Sports Park in East Dublin in 2010. The first phase of the Fallon Sports Park includes two adult softball fields, two little league baseball fields, two synthetic turf soccer fields, four lighted basketball courts, four lighted tennis courts, and a rough grade BMX bike facility. Currently, the city is constructing the second phase of the park, which includes a 90-foot baseball diamond and two additional turf soccer fields, among other amenities.


title: Dublin, California

text: Emerald Glen Park is the largest community park in Dublin. On Memorial Day weekend 2017, the City opened The Wave at Emerald Glen Park, a 31,000-square foot facility which is anchored by an indoor pool for year-round swimming lessons, exercise programs, and recreational use.


title: Dublin, California

text: The City of Dublin is a general law city operating under a City Council / City Manager form of local government. This form of government combines an elected mayor and council and an appointed local government administrator. The City Council elections are nonpartisan. The Mayor serves a two-year term, and Council members serve four-year terms.


title: Dublin, California

text: The Mayor and City Council, as a collegial body, are responsible for setting policy, setting / prioritizing goals and objectives, and approving the budget. The Mayor, with confirmation by the City Council, makes appointments to the city's advisory commissions and committees.


title: Dublin, California

text: The Council appoints the City Manager, who is responsible for the day-to-day administrative operation of the city, including:


title: Dublin, California

text: Additionally, Dublin has introduced the Youth Committee, where teens aged 13–18 can apply for a committee position and meet with the rest of the board.


title: Dublin, California

text: In the House of Representatives, Dublin is part of California's 15th congressional district, represented by Democrat Eric Swalwell. In the State Assembly, it is in , and in .


title: Dublin, California

text: Dublin has 28,798 registered voters with 12,071 (41.9%) registered as Democrats, 5,044 (17.5%) registered as Republicans, and 10,505 (36.5%) decline to state voters.


title: Dublin, California

text: Dublin Unified School District operates the public schools, including seven elementary schools (Amador, Dougherty, Dublin, Frederiksen, John Green, Kolb, and Murray), one k-8 school (Cottonwood), two middle schools (Wells and Fallon), one alternative high school (Valley), and the one following comprehensive high school.


title: Dublin, California

text: A second comprehensive high school is currently under construction in eastern Dublin and is planned to open during the Fall of 2022. The name "Emerald High School" was unanimously chosen as the name of the school by the DUSD Board on August 18, 2022, but is not expected to meet the deadline.


title: Dublin, California

text: Dublin is served by BART with two rail stations, Dublin/Pleasanton and West Dublin/Pleasanton, The West Dublin station began construction in 2007. The project cost $80 million and opened in March 2011. The West Dublin/Pleasanton station includes a hotel, restaurant, 210 apartments, and . of office space.


title: Dublin, California

text: Dublin is situated at the intersection of Interstate 580 and Interstate 680. No US Routes or major California State Highways run directly through Dublin, though California State Route 84 orbits Dublin and surrounding cities from its south to its east.


title: Dublin, California

text: Dublin's closest airport is Livermore Municipal Airport, though the airport is a general aviation airport with no regularly-scheduled commercial service. Through BART, Dublin is accessible to all three major San Francisco Bay Area airports; distance-wise, it is closest to Oakland International Airport.


title: Dublin, California

text: There are full-time 911 emergency services. Police services are contracted to and provided by the Alameda County Sheriff's Office. Fire services and emergency medical services are provided by the city.


title: Dublin, California

text: The California Highway Patrol (CHP) Dublin Office is located off of Gleason Drive. The CHP provides safety, service, and security to the public on freeways/unincorporated highways and on state property.


title: Billy Sunday

text: William Ashley "Billy" Sunday (November 19, 1862 – November 6, 1935) was an American outfielder in baseball's National League and widely considered the most influential American evangelist during the first two decades of the 20th century.


title: Billy Sunday

text: Born into poverty in Iowa, Sunday spent some years at the Iowa Soldiers' Orphans' Home before working at odd jobs and playing for local running and baseball teams. His speed and agility provided him the opportunity to play baseball in the major leagues for eight years, where he was an average hitter and a good fielder known for his base-running.


title: Billy Sunday

text: Converting to evangelical Christianity in the 1880s, Sunday left baseball for the Christian ministry. He gradually developed his skills as a pulpit evangelist in the Midwest and then, during the early 20th century, he became the nation's most famous evangelist with his colloquial sermons and frenetic delivery. Sunday held widely reported campaigns in America's largest cities, and he attracted the largest crowds of any evangelist before the advent of electronic sound systems. He also made a great deal of money and was welcomed into the homes of the wealthy and influential. Sunday was a strong supporter of Prohibition, and his preaching likely played a significant role in the adoption of the Eighteenth Amendment in 1919.


title: Billy Sunday

text: Despite questions about his income, no scandal ever touched Sunday. He was sincerely devoted to his wife, who also managed his campaigns, but his three sons disappointed him. His audiences grew smaller during the 1920s as Sunday grew older, religious revivals became less popular, and alternative sources of entertainment appeared. Nevertheless, Sunday continued to preach and promote conservative Christianity until his death.


title: Billy Sunday

text: Billy Sunday was born near Ames, Iowa. His father, William Sunday, was the son of German immigrants named Sonntag, who had anglicized their name to "Sunday" when they settled in Chambersburg, Pennsylvania. William Sunday was a bricklayer who worked his way to Iowa, where he married Mary Jane Corey, daughter of "Squire" Martin Corey, a local farmer, miller, blacksmith, and wheelwright. William Sunday enlisted in the Iowa Twenty-Third Volunteer Infantry on August 14, 1862. He died four months later of pneumonia at an army camp in Patterson, Missouri, five weeks after the birth of his youngest son, William Ashley. Mary Jane Sunday and her children moved in with her parents for a few years, and young Billy became close to his grandparents and especially his grandmother. Mary Jane Sunday later remarried, but her second husband soon deserted the family.


title: Billy Sunday

text: When Billy Sunday was ten years old, his impoverished mother sent him and an older brother to the Soldiers' Orphans Home in Glenwood, Iowa, and later to the Iowa Soldiers' Orphans' Home in Davenport, Iowa. At the orphanage, Sunday gained orderly habits, a decent primary education, and the realization that he was a good athlete.


title: Billy Sunday

text: By fourteen, Sunday was shifting for himself. In Nevada, Iowa, he worked for Colonel John Scott, a former lieutenant governor, tending Shetland ponies and doing other farm chores. The Scotts provided Sunday a good home and the opportunity to attend Nevada High School. Although Sunday never received a high school diploma, by 1880 he was better educated than many of his contemporaries.


title: Billy Sunday

text: In 1880, Sunday relocated to Marshalltown, Iowa, where, because of his athleticism, he had been recruited for a fire brigade team. In Marshalltown, Sunday worked at odd jobs, competed in fire brigade tournaments, and played for the town baseball team.


title: Billy Sunday

text: Sunday's professional baseball career was launched by Cap Anson, a Marshalltown native and future Hall of Famer, after his aunt, an avid fan of the Marshalltown team, gave him an enthusiastic account of Sunday's prowess. In 1883, on Anson's recommendation, A.G. Spalding, president of the Chicago White Stockings, signed Sunday to the defending National League champions.


title: Billy Sunday

text: Sunday struck out four times in his first game, and there were seven more strikeouts and three more games before he got a hit. During his first four seasons with Chicago, he was a part-time player, taking Mike "King" Kelly's place in right field when Kelly served as catcher.


title: Billy Sunday

text: Sunday's speed was his greatest asset, and he displayed it both on the basepaths and in the outfield. In 1885, the White Stockings arranged a race between Sunday and Arlie Latham, the fastest runner in the American Association. Sunday won the hundred-yard dash by about ten feet.


title: Billy Sunday

text: Sunday's personality, demeanor, and athleticism made him popular with the fans, as well as with his teammates. Manager Cap Anson considered Sunday reliable enough to make him the team's business manager, which included such duties as handling the ticket receipts and paying the team's travel expenses.


title: Billy Sunday

text: In 1887, when Kelly was sold to another team, Sunday became Chicago's regular right fielder, but an injury limited his playing time to fifty games. During the following winter Sunday was sold to the Pittsburgh Alleghenys for the 1888 season. He was their starting center fielder, playing a full season for the first time in his career. The crowds in Pittsburgh took to Sunday immediately; one reporter wrote that "the whole town is wild over Sunday." Although Pittsburgh had a losing team during the 1888 and 1889 seasons, Sunday performed well in center field and was among the league leaders in stolen bases.


title: Billy Sunday

text: In 1890, a labor dispute led to the formation of a new league, composed of most of the better players from the National League. Although he was invited to join the competing league, Sunday's conscience would not allow him to break the reserve clause, which allowed Pittsburgh to retain the rights to Sunday after his contract expired. Sunday was named team captain, and he was their star player, but the team suffered one of the worst seasons in baseball history. By August the team had no money to meet its payroll, and Sunday was traded to the Philadelphia Phillies for two players and $1,000 in cash.


title: Billy Sunday

text: The Philadelphia team had an opportunity to win the National League pennant, and the owners hoped that adding Sunday to the roster would improve their chances. Although Sunday played well in his thirty-one games with Philadelphia, the team finished in third place.


title: Billy Sunday

text: In March 1891, Sunday requested and was granted a release from his contract with the Philadelphia ball club. Over his career, Sunday was never much of a hitter: his batting average was .248 over 499 games, about the median for the 1880s. In his best season, in 1887, Sunday hit .291, ranking 17th in the league. He was an exciting but inconsistent fielder. In the days before outfielders wore gloves, Sunday was noted for thrilling catches featuring long sprints and athletic dives, but he also committed a great many errors. Sunday was best known as an exciting base-runner, regarded by his peers as one of the fastest in the game, even though he never placed better than third in the National League in stolen bases.


title: Billy Sunday

text: Sunday remained a prominent baseball fan throughout his life. He gave interviews and opinions about baseball to the popular press; he frequently umpired minor league and amateur games in the cities where he held revivals; and he attended baseball games whenever he could, including a 1935 World Series game two months before he died.


title: Billy Sunday

text: On a Sunday afternoon in Chicago, during either the 1886 or 1887 baseball season, Sunday and several of his teammates were out on the town on their day off. At one street corner, they stopped to listen to a gospel preaching team from the Pacific Garden Mission. Attracted by the hymns he had heard his mother sing, Sunday began attending services at the mission. After talking with a former society matron who worked there, Sundayafter some struggle on his partdecided to become a Christian. He began attending the fashionable Jefferson Park Presbyterian Church, a congregation handy to both the ball park and his rented room.


title: Billy Sunday

text: Although he socialized with his teammates and sometimes gambled, Sunday was never a heavy drinker. In his autobiography, he said, "I never drank much. I was never drunk but four times in my life. ... I used to go to the saloons with the baseball players, and while they would drink highballs and gin fizzes and beer, I would take lemonade." Following his conversion, Sunday denounced drinking, swearing, and gambling, and he changed his behavior, which was recognized by both teammates and fans. Shortly thereafter, Sunday began speaking in churches and at YMCAs.


title: Billy Sunday

text: In 1886, Sunday was introduced at Jefferson Park Presbyterian Church to Helen Amelia "Nell" Thompson, daughter of the owner of one of Chicago's largest dairy products businesses. Although Sunday was immediately smitten with her, both had serious on-going relationships that bordered on engagements. Furthermore, Nell Thompson had grown to maturity in a much more privileged environment than had Sunday, and her father strongly discouraged the courtship, viewing all professional baseball players as "transient ne'er-do-wells who were unstable and destined to be misfits once they were too old to play." Nevertheless, Sunday pursued and eventually married her. On several occasions, Sunday said, "She was a Presbyterian, so I am a Presbyterian. Had she been a Catholic, I would have been a Catholicbecause I was hot on the trail of Nell." Her mother liked Sunday from the start and weighed in on his side, and her father finally relented. The couple was married on September 5, 1888.


title: Billy Sunday

text: In the spring of 1891, Sunday turned down a baseball contract for $3,500 a year to accept a position with the Chicago YMCA at $83 per month. Sunday's job title at the YMCA was Assistant Secretary, yet the position involved a great deal of ministerial work. It proved to be good preparation for his later evangelistic career. For three years Sunday visited the sick, prayed with the troubled, counseled the suicidal, and visited saloons to invite patrons to evangelistic meetings.


title: Billy Sunday

text: In 1893, Sunday became the full-time assistant to J. Wilbur Chapman, one of the best known evangelists in the United States at the time. Chapman was well educated and was a meticulous dresser, "suave and urbane." Personally shy, like Sunday, Chapman commanded respect in the pulpit both because of his strong voice and his sophisticated demeanor. Sunday's job as Chapman's advance man was to precede the evangelist to cities in which he was scheduled to preach, organize prayer meetings and choirs, and in general take care of necessary details. When tents were used, Sunday would often help erect them.


title: Billy Sunday

text: By listening to Chapman preach night after night, Sunday received a valuable course in homiletics. Chapman also critiqued Sunday's own attempts at evangelistic preaching and showed him how to put a good sermon together. Further, Chapman encouraged Sunday's theological development, especially by emphasizing the importance of prayer and by helping to "reinforce Billy's commitment to conservative biblical Christianity."


title: Billy Sunday

text: When Chapman unexpectedly returned to the pastorate in 1896, Sunday struck out on his own, beginning with meetings in tiny Garner, Iowa. For the next twelve years Sunday preached in approximately seventy communities, most of them in Iowa and Illinois. Sunday referred to these towns as the "kerosene circuit" because, unlike Chicago, most were not yet electrified. Towns often booked Sunday meetings informally, sometimes by sending a delegation to hear him preach and then telegraphing him while he was holding services somewhere else.


title: Billy Sunday

text: Sunday also took advantage of his reputation as a baseball player to generate advertising for his meetings. In 1907 in Fairfield, Iowa, Sunday organized local businesses into two baseball teams and scheduled a game between them. Sunday came dressed in his professional uniform and played on both sides. Although baseball was his primary means of publicity, Sunday also once hired a circus giant to serve as an usher.


title: Billy Sunday

text: When Sunday began to attract crowds larger than could be accommodated in rural churches or town halls, he pitched rented canvas tents. Again, Sunday did much of the physical work of putting them up, manipulating ropes during storms, and seeing to their security by sleeping in them at night. Not until 1905 was he well-off enough to hire his own advance man.


title: Billy Sunday

text: In 1906, an October snowstorm in Salida, Colorado, destroyed Sunday's tenta special disaster because revivalists were typically paid with a freewill offering at the end of their meetings. Thereafter he insisted that towns build him temporary wooden tabernacles at their expense. The tabernacles were comparatively costly to build (although most of the lumber could be salvaged and resold at the end of the meetings), and locals had to put up the money for them in advance. This change in Sunday's operation began to push the finances of the campaign to the fore. At least at first, raising tabernacles provided good public relations for the coming meetings as townspeople joined together in what was effectively a giant barnraising. Sunday built rapport by participating in the process, and the tabernacles were also a status symbol, because they had previously been built only for major evangelists such as Chapman.


title: Billy Sunday

text: Eleven years into Sunday's evangelistic career, both he and his wife had been pushed to their emotional limits. Long separations had exacerbated his natural feelings of inadequacy and insecurity. As a product of a childhood that could well be described as a series of losses, he was extremely dependent on his wife's love and encouragement. For her part, Nell found it increasingly difficult to handle household responsibilities, the needs of four children (including a newborn), and the long-distance emotional welfare of her husband. His ministry was also expanding, and he needed an administrator, a job for which his wife was ideally suited. In 1908, the Sundays decided to entrust their children to a nanny so that Nell could manage the revival campaigns.


title: Billy Sunday

text: Nell Sunday transformed her husband's out-of-the-back-pocket organization into a "nationally renowned phenomenon." New personnel were hired, and by the New York campaign of 1917, the Sundays had a paid staff of twenty-six. There were musicians, custodians, and advance men; but the Sundays also hired Bible teachers of both genders, who among other responsibilities, held daytime meetings at schools and shops and encouraged their audiences to attend the main tabernacle services in the evenings. The most significant of these new staff members were Homer Rodeheaver, an exceptional song leader and music director who worked with the Sundays for almost twenty years beginning in 1910, and Virginia Healey Asher, who (besides regularly singing duets with Rodeheaver) directed the women's ministries, especially the evangelization of young working women.


title: Billy Sunday

text: With his wife administering the campaign organization, Sunday was free to do what he did best: compose and deliver colloquial sermons. Typically, Homer Rodeheaver would first warm up the crowd with congregational singing that alternated with numbers from gigantic choirs and music performed by the staff. When Sunday felt the moment right, he would launch into his message. Sunday gyrated, stood on the pulpit, ran from one end of the platform to the other, and dove across the stage, pretending to slide into home plate. Sometimes he even smashed chairs to emphasize his points. His sermon notes had to be printed in large letters so that he could catch a glimpse of them as he raced by the pulpit. In messages attacking sexual sin to groups of men only, Sunday could be graphic for the era.


title: Billy Sunday

text: Homer Rodeheaver said that "One of these sermons, until he tempered it down a little, had one ten-minute period in it where from two to twelve men fainted and had to be carried out every time I heard him preach it." Some religious and social leaders criticized Sunday's exaggerated gestures as well as the slang and colloquialisms that filled his sermons, but audiences clearly enjoyed them.


title: Billy Sunday

text: In 1907, journalist Lindsay Denison complained that Sunday preached "the old, old doctrine of damnation". Denison wrote, "In spite of his conviction that the truly religious man should take his religion joyfully, he gets his results by inspiring fear and gloom in the hearts of sinners. The fear of death, with torment beyond it—intensified by examples of the frightful deathbeds of those who have carelessly or obdurately put off salvation until it is too late—it is with this mighty menace that he drives sinners into the fold." But Sunday himself told reporters "with ill-concealed annoyance" that his revivals had "no emotionalism." Caricatures compared him to the extravagances of mid-nineteenth-century camp meetings, as in the famous drawing "Billy Sunday" by George Bellows. Sunday told one reporter that he believed that people could "be converted without any fuss," and, at Sunday's meetings, "instances of spasm, shakes, or fainting fits caused by hysteria were few and far between."


title: Billy Sunday

text: Crowd noise, especially coughing and crying babies, was a significant impediment to Sunday's preaching because the wooden tabernacles were so acoustically live. During his preliminaries, Rodeheaver often instructed audiences about how to muffle their coughs. Nurseries were always provided, infants forbidden, and Sunday sometimes appeared rude in his haste to rid the hall of noisy children who had slipped through the ushers.


title: Billy Sunday

text: Tabernacle floors were covered with sawdust to dampen the noise of shuffling feet (as well as for its pleasant smell and its ability to hold down the dust of dirt floors), and walking to the front at the preacher's invitation became known as "hitting the sawdust trail." The term was first used in a Sunday campaign in Bellingham, Washington, in 1910. Apparently, "hitting the sawdust trail" had first been used by loggers in the Pacific Northwest to describe following home a trail of previously dropped sawdust through an uncut forest — described by Nell Sunday as a metaphor for coming from "a lost condition to a saved condition."


title: Billy Sunday

text: By 1910, Sunday began to conduct meetings (usually longer than a month) in small cities like Youngstown, Wilkes-Barre, South Bend, and Denver, and then finally, between 1915 and 1917, the major cities of Philadelphia, Syracuse, Kansas City, Detroit, Boston, Buffalo, and New York City. During the 1910s, Sunday was front-page news in the cities where he held campaigns. Newspapers often printed his sermons in full, and during World War I, local coverage of his campaigns often surpassed that of the war. Sunday was the subject of over sixty articles in major periodicals, and he was a staple of the religious press regardless of denomination.


title: Billy Sunday

text: Over the course of his career, Sunday probably preached to more than one hundred million people face-to-face—and, to the great majority, without electronic amplification. Vast numbers "hit the sawdust trail." Although the usual total given for those who came forward at invitations is an even million, one modern historian estimates the true figure to be closer to 1,250,000. Sunday did not preach to a hundred million different individuals but to many of the same people repeatedly over the course of a campaign. Before his death, Sunday estimated that he had preached nearly 20,000 sermons, an average of 42 per month from 1896 to 1935. During his heyday, when he was preaching more than twenty times each week, his crowds were often huge. Even in 1923, well into the period of his decline, 479,300 people attended the 79 meetings of the six-week 1923 Columbia, South Carolina, campaign–23 times the white population of Columbia. Nevertheless,"trail hitters" were not necessarily conversions (or even "reconsecrations") to Christianity. Sometimes whole groups of club members came forward en masse at Sunday's prodding. By 1927, Rodeheaver was complaining that Sunday's invitations had become so general that they were meaningless.


title: Billy Sunday

text: Large crowds and an efficient organization meant that Sunday, the former resident of an orphan home, was soon netting hefty offerings. The first questions about Sunday's income were apparently raised during the Columbus, Ohio, campaign at the turn of 1912–13. During the Pittsburgh campaign a year later, Sunday spoke four times per day and effectively made $217 per sermon or $870 a day at a time when the average gainfully employed worker made $836 per year. The major cities of Chicago, Philadelphia, Baltimore, Boston, and New York City gave Sunday even larger offerings. Sunday donated Chicago's offering of $58,000 to Pacific Garden Mission and the $120,500 New York offering to war charities. Nevertheless, between 1908 and 1920, the Sundays earned over a million dollars; an average worker during the same period earned less than $14,000.