Banded - Found is coastal drainages of Alabama and Florida from Conecuh River to the Ochlockonee system, male banded topminnows are olive green with orange highlighting the upper gill covers.
Bayou - The bayou topminnow has rows of dots and vertical bars, and it has a limited distribution, ranging from the Escatawpa and Mobile basins west to the Lake Pontchartrain drainage in Louisiana.
Blackspotted - Blackspotted topminnows are characterized by a dark lateral stripe extending from the mouth to the caudal fin base and by small, distinct black spots along the back and upper sides (a useful characteristic for separating the blackspotted topminnow from the blackstripe topminnow).
Blackstripe - The blackstripe topminnow can usually be distinguished from the blackspotted topminnow by the absence of small dark dots along the upper sides and back.
Bluefin Killifish - The colorful bluefin killifish is found in Georgia, Florida, and only one pond in extreme southeast Alabama.
Golden - When not breeding the golden topminnow is similar in appearance to the banded topminnow; in Alabama, the golden topminnow is limited to tributaries and backwaters of the Mobile Delta, Mobile Bay, and coastal lowland systems.
Northern Starhead - The northern starhead topminnow is difficult to identify because of the sexual dimorphism.
Northern Studfish - The northern studfish is a colorful topminnow that occurs in the Ozark and Ouachita mountains, upland regions of the Tennessee, Cumberland, and Green river drainages, and in isolated populations in Indiana and Mississippi.
Pygmy Killifish - Perhaps Alabama's smallest fish, this fish is very rare in Alabama, but more common in Georgia and Florida.
Rainwater Killifish - One of Alabama's smallest fish, it can be confused with the mosquitofish; the edges of the scales of rainwater killifish are edged in black, giving them a diamond-shaped appearance.
Russetfin - In Gulf coastal drainages, Fundulus escambiae is distributed from the Perdido River drainage eastward to the Santa Fe River in Florida.
Southern Starhead - The southern starhead topminnow is listed as a distinct species because individuals in Alabama are distinguishable from the bayou topminnow on the basis of body coloration.
Southern Studfish - Found in the Coosa system and some lower Alabama River tributaries, the southern studfish is most closely related to the northern studfish and stippled studfish.
Stippled Studfish - Only found in the Mobile basin, the stippled studfish is characterized by a series of darkened spots on individual scales that form a unique stippled pattern.
Whiteline - The whiteline topminnow is an extinct fish that was first found in Spring Creek, Madison County, Alabama, now a part of Huntsville.