diff --git "a/code/Psoriasis/GSE254707.ipynb" "b/code/Psoriasis/GSE254707.ipynb" new file mode 100644--- /dev/null +++ "b/code/Psoriasis/GSE254707.ipynb" @@ -0,0 +1,418 @@ +{ + "cells": [ + { + "cell_type": "code", + "execution_count": 1, + "id": "c08e73ac", + "metadata": { + "execution": { + "iopub.execute_input": "2025-03-25T03:44:23.035895Z", + "iopub.status.busy": "2025-03-25T03:44:23.035434Z", + "iopub.status.idle": "2025-03-25T03:44:23.204343Z", + "shell.execute_reply": "2025-03-25T03:44:23.203954Z" + } + }, + "outputs": [], + "source": [ + "import sys\n", + "import os\n", + "sys.path.append(os.path.abspath(os.path.join(os.getcwd(), '../..')))\n", + "\n", + "# Path Configuration\n", + "from tools.preprocess import *\n", + "\n", + "# Processing context\n", + "trait = \"Psoriasis\"\n", + "cohort = \"GSE254707\"\n", + "\n", + "# Input paths\n", + "in_trait_dir = \"../../input/GEO/Psoriasis\"\n", + "in_cohort_dir = \"../../input/GEO/Psoriasis/GSE254707\"\n", + "\n", + "# Output paths\n", + "out_data_file = \"../../output/preprocess/Psoriasis/GSE254707.csv\"\n", + "out_gene_data_file = \"../../output/preprocess/Psoriasis/gene_data/GSE254707.csv\"\n", + "out_clinical_data_file = \"../../output/preprocess/Psoriasis/clinical_data/GSE254707.csv\"\n", + "json_path = \"../../output/preprocess/Psoriasis/cohort_info.json\"\n" + ] + }, + { + "cell_type": "markdown", + "id": "764cb33a", + "metadata": {}, + "source": [ + "### Step 1: Initial Data Loading" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "a76e685a", + "metadata": { + "execution": { + "iopub.execute_input": "2025-03-25T03:44:23.205880Z", + "iopub.status.busy": "2025-03-25T03:44:23.205725Z", + "iopub.status.idle": "2025-03-25T03:44:23.364048Z", + "shell.execute_reply": "2025-03-25T03:44:23.363640Z" + } + }, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Background Information:\n", + "!Series_title\t\"Single-cell transcriptomic analysis identifies infiltrating plasmacytoid dendritic cells in psoriasis epidermis\"\n", + "!Series_summary\t\"The study focuses on the cellular composition of the psoriasis epidermis, using single-cell transcriptomics to identify cell subsets and their interactions in both healthy and psoriatic skin. The research uncovers three keratinocyte populations and seven immune cell subsets exclusive to psoriatic lesions. A significant finding is the identification of a previously undetected population of plasmacytoid dendritic cells (pDCs) in the psoriatic epidermis, suggesting their role in the disease's pathogenesis. The study also highlights enhanced keratinocyte-immune cell interactions in psoriatic lesions, contributing to our understanding of psoriasis at the cellular level.\"\n", + "!Series_overall_design\t\"Epidermal sheets from biopsies obtained from lesional and nonlesional skin of 5 untreated psoriasis patients and 3 healthy donors were subjected to partial dissociation preserving physical cell-cell interactions, followed by separation of CD45neg and CD45pos subsets by flow cytometry. Single-cell RNA sequencing was performed followed by Cell Interaction by Multiplet Sequencing (CIM-seq) analysis.\"\n", + "Sample Characteristics Dictionary:\n", + "{0: ['tissue: Skin'], 1: ['ncount rna: 1675950', 'ncount rna: 977978', 'ncount rna: 491562', 'ncount rna: 527568', 'ncount rna: 521680', 'ncount rna: 1316001', 'ncount rna: 498466', 'ncount rna: 942803', 'ncount rna: 860964', 'ncount rna: 2309880', 'ncount rna: 1078236', 'ncount rna: 318687', 'ncount rna: 1557646', 'ncount rna: 166641', 'ncount rna: 321270', 'ncount rna: 329655', 'ncount rna: 874856', 'ncount rna: 1098309', 'ncount rna: 742060', 'ncount rna: 401999', 'ncount rna: 1056068', 'ncount rna: 1442476', 'ncount rna: 1557346', 'ncount rna: 1339432', 'ncount rna: 544634', 'ncount rna: 658219', 'ncount rna: 1057940', 'ncount rna: 781377', 'ncount rna: 460421', 'ncount rna: 900895'], 2: ['nfeature rna: 4558', 'nfeature rna: 3881', 'nfeature rna: 2501', 'nfeature rna: 2576', 'nfeature rna: 3187', 'nfeature rna: 4094', 'nfeature rna: 2443', 'nfeature rna: 3368', 'nfeature rna: 2802', 'nfeature rna: 5030', 'nfeature rna: 3238', 'nfeature rna: 1736', 'nfeature rna: 5278', 'nfeature rna: 1569', 'nfeature rna: 1784', 'nfeature rna: 2008', 'nfeature rna: 3668', 'nfeature rna: 3715', 'nfeature rna: 3132', 'nfeature rna: 1881', 'nfeature rna: 3172', 'nfeature rna: 4218', 'nfeature rna: 4263', 'nfeature rna: 3301', 'nfeature rna: 3319', 'nfeature rna: 2167', 'nfeature rna: 3598', 'nfeature rna: 3759', 'nfeature rna: 1938', 'nfeature rna: 4327'], 3: ['percent.ercc: 0.0145406628764158', 'percent.ercc: 0.0281649692295003', 'percent.ercc: 0.0507838976095902', 'percent.ercc: 0.0576265252425294', 'percent.ercc: 0.0500729270420027', 'percent.ercc: 0.0245312416610827', 'percent.ercc: 0.0742146074197892', 'percent.ercc: 0.0430348730868117', 'percent.ercc: 0.0406466378885766', 'percent.ercc: 0.0110426463989807', 'percent.ercc: 0.0245509664602207', 'percent.ercc: 0.0988199630690575', 'percent.ercc: 0.0125782256346799', 'percent.ercc: 0.1054513245833', 'percent.ercc: 0.0908576392917479', 'percent.ercc: 0.0887567585497728', 'percent.ercc: 0.0272244090110525', 'percent.ercc: 0.0359435702887318', 'percent.ercc: 0.0445657310090604', 'percent.ercc: 0.160993619778894', 'percent.ercc: 0.0316073640784311', 'percent.ercc: 0.0179408344612167', 'percent.ercc: 0.0192930276961403', 'percent.ercc: 0.0227375535898252', 'percent.ercc: 0.0358839096839292', 'percent.ercc: 0.0658750533962877', 'percent.ercc: 0.0289513732214025', 'percent.ercc: 0.0288965832658073', 'percent.ercc: 0.0884918435223313', 'percent.ercc: 0.0275145080225911'], 4: ['donor: P185', 'donor: P186', 'donor: P187', 'donor: P189', 'donor: H061', 'donor: H062', 'donor: P190', 'donor: H066'], 5: ['diagnosis: Psoriasis', 'diagnosis: Healthy'], 6: ['region: PP', 'region: PN', 'region: H'], 7: ['fsc.a.x: 356956.3', 'fsc.a.x: 687320.9', 'fsc.a.x: 169972.7', 'fsc.a.x: 477608.3', 'fsc.a.x: 294204.2', 'fsc.a.x: 190021.9', 'fsc.a.x: 185699.3', 'fsc.a.x: 95900.68', 'fsc.a.x: 646659.1', 'fsc.a.x: 492049', 'fsc.a.x: 599958.9', 'fsc.a.x: 102222.7', 'fsc.a.x: 399800.8', 'fsc.a.x: 340720.4', 'fsc.a.x: 404236.3', 'fsc.a.x: 292051.7', 'fsc.a.x: 346072.2', 'fsc.a.x: 132484.1', 'fsc.a.x: 181049.2', 'fsc.a.x: 275025.3', 'fsc.a.x: 744250.3', 'fsc.a.x: 706905.7', 'fsc.a.x: 725823.6', 'fsc.a.x: 396497.9', 'fsc.a.x: 129801.6', 'fsc.a.x: 298553', 'fsc.a.x: 145781.4', 'fsc.a.x: 129356.5', 'fsc.a.x: 317775.4', 'fsc.a.x: 427888.6'], 8: ['fsc.h.x: 172729.8', 'fsc.h.x: 335093.9', 'fsc.h.x: 94104.64', 'fsc.h.x: 300651.7', 'fsc.h.x: 141514.2', 'fsc.h.x: 75388.32', 'fsc.h.x: 100225.4', 'fsc.h.x: 60911.2', 'fsc.h.x: 268189.6', 'fsc.h.x: 269056.5', 'fsc.h.x: 203911.7', 'fsc.h.x: 73202.08', 'fsc.h.x: 133399.8', 'fsc.h.x: 247568.2', 'fsc.h.x: 309103.2', 'fsc.h.x: 243844.2', 'fsc.h.x: 178025.1', 'fsc.h.x: 78196.16', 'fsc.h.x: 107447.2', 'fsc.h.x: 215886.7', 'fsc.h.x: 328596.8', 'fsc.h.x: 334587.7', 'fsc.h.x: 299673.9', 'fsc.h.x: 154773.9', 'fsc.h.x: 82956.16', 'fsc.h.x: 205288.2', 'fsc.h.x: 75454.4', 'fsc.h.x: 78809.92', 'fsc.h.x: 244268.6', 'fsc.h.x: 209559.8'], 9: ['fsc.w.x: 322', 'fsc.w.x: 310', 'fsc.w.x: 216', 'fsc.w.x: 268', 'fsc.w.x: 271', 'fsc.w.x: 248', 'fsc.w.x: 227', 'fsc.w.x: 136', 'fsc.w.x: 357', 'fsc.w.x: 287', 'fsc.w.x: 389', 'fsc.w.x: 157', 'fsc.w.x: 373', 'fsc.w.x: 239', 'fsc.w.x: 240', 'fsc.w.x: 224', 'fsc.w.x: 294', 'fsc.w.x: 187', 'fsc.w.x: 212', 'fsc.w.x: 226', 'fsc.w.x: 350', 'fsc.w.x: 314', 'fsc.w.x: 347', 'fsc.w.x: 368', 'fsc.w.x: 185', 'fsc.w.x: 246', 'fsc.w.x: 192', 'fsc.w.x: 232', 'fsc.w.x: 302', 'fsc.w.x: 182'], 10: ['class: doublet', 'class: singlet'], 11: ['seurat clusters: NA', 'seurat clusters: 3', 'seurat clusters: 1', 'seurat clusters: 0', 'seurat clusters: 6', 'seurat clusters: 2', 'seurat clusters: 11', 'seurat clusters: 4', 'seurat clusters: 5', 'seurat clusters: 8', 'seurat clusters: 7', 'seurat clusters: 9', 'seurat clusters: 12', 'seurat clusters: 13', 'seurat clusters: 10'], 12: ['celltype: NA', 'celltype: Activated suprabasal keratinocytes', 'celltype: mDC', 'celltype: Mac/MoDC', 'celltype: Activated basal keratinocytes', 'celltype: Treg', 'celltype: Activated mitotic keratinocytes', 'celltype: T lymphocytes', 'celltype: Melanocytes', 'celltype: LC', 'celltype: ILC', 'celltype: Proliferative CD8', 'celltype: Activated Transition keratinocytes', 'celltype: Tc (cytokine-)', 'celltype: Tc (cytokine+)', 'celltype: Basal keratinocytes III', 'celltype: Suprabasal keratinocytes I', 'celltype: Transition keratinocytes', 'celltype: Progenitor keratinocytes', 'celltype: Suprabasal keratinocytes II', 'celltype: Channel keratinocytes', 'celltype: Basal keratinocytes II', 'celltype: Basal keratinocytes I', 'celltype: Th1', 'celltype: ILC/NK', 'celltype: Th17', 'celltype: pDC', 'celltype: Mitotic keratinocytes']}\n" + ] + } + ], + "source": [ + "from tools.preprocess import *\n", + "# 1. Identify the paths to the SOFT file and the matrix file\n", + "soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir)\n", + "\n", + "# 2. Read the matrix file to obtain background information and sample characteristics data\n", + "background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design']\n", + "clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1']\n", + "background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes)\n", + "\n", + "# 3. Obtain the sample characteristics dictionary from the clinical dataframe\n", + "sample_characteristics_dict = get_unique_values_by_row(clinical_data)\n", + "\n", + "# 4. Explicitly print out all the background information and the sample characteristics dictionary\n", + "print(\"Background Information:\")\n", + "print(background_info)\n", + "print(\"Sample Characteristics Dictionary:\")\n", + "print(sample_characteristics_dict)\n" + ] + }, + { + "cell_type": "markdown", + "id": "dd06dd66", + "metadata": {}, + "source": [ + "### Step 2: Dataset Analysis and Clinical Feature Extraction" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "f045416d", + "metadata": { + "execution": { + "iopub.execute_input": "2025-03-25T03:44:23.365377Z", + "iopub.status.busy": "2025-03-25T03:44:23.365258Z", + "iopub.status.idle": "2025-03-25T03:44:23.547877Z", + "shell.execute_reply": "2025-03-25T03:44:23.547360Z" + } + }, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Preview of extracted clinical data:\n", + "{'GSM8049207': [1.0], 'GSM8049208': [1.0], 'GSM8049209': [1.0], 'GSM8049210': [1.0], 'GSM8049211': [1.0], 'GSM8049212': [1.0], 'GSM8049213': [1.0], 'GSM8049214': [1.0], 'GSM8049215': [1.0], 'GSM8049216': [1.0], 'GSM8049217': [1.0], 'GSM8049218': [1.0], 'GSM8049219': [1.0], 'GSM8049220': [1.0], 'GSM8049221': [1.0], 'GSM8049222': [1.0], 'GSM8049223': [1.0], 'GSM8049224': [1.0], 'GSM8049225': [1.0], 'GSM8049226': [1.0], 'GSM8049227': [1.0], 'GSM8049228': [1.0], 'GSM8049229': [1.0], 'GSM8049230': [1.0], 'GSM8049231': [1.0], 'GSM8049232': [1.0], 'GSM8049233': [1.0], 'GSM8049234': [1.0], 'GSM8049235': [1.0], 'GSM8049236': [1.0], 'GSM8049237': [1.0], 'GSM8049238': [1.0], 'GSM8049239': [1.0], 'GSM8049240': [1.0], 'GSM8049241': [1.0], 'GSM8049242': [1.0], 'GSM8049243': [1.0], 'GSM8049244': [1.0], 'GSM8049245': [1.0], 'GSM8049246': [1.0], 'GSM8049247': [1.0], 'GSM8049248': [1.0], 'GSM8049249': [1.0], 'GSM8049250': [1.0], 'GSM8049251': [1.0], 'GSM8049252': [1.0], 'GSM8049253': [1.0], 'GSM8049254': [1.0], 'GSM8049255': [1.0], 'GSM8049256': [1.0], 'GSM8049257': [1.0], 'GSM8049258': [1.0], 'GSM8049259': [1.0], 'GSM8049260': [1.0], 'GSM8049261': [1.0], 'GSM8049262': [1.0], 'GSM8049263': [1.0], 'GSM8049264': [1.0], 'GSM8049265': [1.0], 'GSM8049266': [1.0], 'GSM8049267': [1.0], 'GSM8049268': [1.0], 'GSM8049269': [1.0], 'GSM8049270': [1.0], 'GSM8049271': [1.0], 'GSM8049272': [1.0], 'GSM8049273': [1.0], 'GSM8049274': [1.0], 'GSM8049275': [1.0], 'GSM8049276': [1.0], 'GSM8049277': [1.0], 'GSM8049278': [1.0], 'GSM8049279': [1.0], 'GSM8049280': [1.0], 'GSM8049281': [1.0], 'GSM8049282': [1.0], 'GSM8049283': [1.0], 'GSM8049284': [1.0], 'GSM8049285': [1.0], 'GSM8049286': [1.0], 'GSM8049287': [1.0], 'GSM8049288': [1.0], 'GSM8049289': [1.0], 'GSM8049290': [1.0], 'GSM8049291': [1.0], 'GSM8049292': [1.0], 'GSM8049293': [1.0], 'GSM8049294': [1.0], 'GSM8049295': [1.0], 'GSM8049296': [1.0], 'GSM8049297': [1.0], 'GSM8049298': [1.0], 'GSM8049299': [1.0], 'GSM8049300': [1.0], 'GSM8049301': [1.0], 'GSM8049302': [1.0], 'GSM8049303': [1.0], 'GSM8049304': [1.0], 'GSM8049305': [1.0], 'GSM8049306': [1.0], 'GSM8049307': [1.0], 'GSM8049308': [1.0], 'GSM8049309': [1.0], 'GSM8049310': [1.0], 'GSM8049311': [1.0], 'GSM8049312': [1.0], 'GSM8049313': [1.0], 'GSM8049314': [1.0], 'GSM8049315': [1.0], 'GSM8049316': [1.0], 'GSM8049317': [1.0], 'GSM8049318': [1.0], 'GSM8049319': [1.0], 'GSM8049320': [1.0], 'GSM8049321': [1.0], 'GSM8049322': [1.0], 'GSM8049323': [1.0], 'GSM8049324': [1.0], 'GSM8049325': [1.0], 'GSM8049326': [1.0], 'GSM8049327': [1.0], 'GSM8049328': [1.0], 'GSM8049329': [1.0], 'GSM8049330': [1.0], 'GSM8049331': [1.0], 'GSM8049332': [1.0], 'GSM8049333': [1.0], 'GSM8049334': [1.0], 'GSM8049335': [1.0], 'GSM8049336': [1.0], 'GSM8049337': [1.0], 'GSM8049338': [1.0], 'GSM8049339': [1.0], 'GSM8049340': [1.0], 'GSM8049341': [1.0], 'GSM8049342': [1.0], 'GSM8049343': [1.0], 'GSM8049344': [1.0], 'GSM8049345': [1.0], 'GSM8049346': [1.0], 'GSM8049347': [1.0], 'GSM8049348': [1.0], 'GSM8049349': [1.0], 'GSM8049350': [1.0], 'GSM8049351': [1.0], 'GSM8049352': [1.0], 'GSM8049353': [1.0], 'GSM8049354': [1.0], 'GSM8049355': [1.0], 'GSM8049356': [1.0], 'GSM8049357': [1.0], 'GSM8049358': [1.0], 'GSM8049359': [1.0], 'GSM8049360': [1.0], 'GSM8049361': [1.0], 'GSM8049362': [1.0], 'GSM8049363': [1.0], 'GSM8049364': [1.0], 'GSM8049365': [1.0], 'GSM8049366': [1.0], 'GSM8049367': [1.0], 'GSM8049368': [1.0], 'GSM8049369': [1.0], 'GSM8049370': [1.0], 'GSM8049371': [1.0], 'GSM8049372': [1.0], 'GSM8049373': [1.0], 'GSM8049374': [1.0], 'GSM8049375': [1.0], 'GSM8049376': [1.0], 'GSM8049377': [1.0], 'GSM8049378': [1.0], 'GSM8049379': [1.0], 'GSM8049380': [1.0], 'GSM8049381': [1.0], 'GSM8049382': [1.0], 'GSM8049383': [1.0], 'GSM8049384': [1.0], 'GSM8049385': [1.0], 'GSM8049386': [1.0], 'GSM8049387': [1.0], 'GSM8049388': [1.0], 'GSM8049389': [1.0], 'GSM8049390': [1.0], 'GSM8049391': [1.0], 'GSM8049392': [1.0], 'GSM8049393': [1.0], 'GSM8049394': [1.0], 'GSM8049395': [1.0], 'GSM8049396': [1.0], 'GSM8049397': [1.0], 'GSM8049398': [1.0], 'GSM8049399': [1.0], 'GSM8049400': [1.0], 'GSM8049401': [1.0], 'GSM8049402': [1.0], 'GSM8049403': [1.0], 'GSM8049404': [1.0], 'GSM8049405': [1.0], 'GSM8049406': [1.0]}\n", + "Clinical data saved to ../../output/preprocess/Psoriasis/clinical_data/GSE254707.csv\n" + ] + } + ], + "source": [ + "import os\n", + "import pandas as pd\n", + "import numpy as np\n", + "import json\n", + "from typing import Dict, Any, Optional, Callable\n", + "\n", + "# 1. Analyze gene expression data availability\n", + "is_gene_available = True # The dataset contains RNA-seq data based on the series information\n", + "\n", + "# 2. Variable Availability and Data Type Conversion\n", + "\n", + "# 2.1 Identify row keys for each variable\n", + "trait_row = 5 # 'diagnosis: Psoriasis', 'diagnosis: Healthy'\n", + "age_row = None # Age information is not available in the provided data\n", + "gender_row = None # Gender information is not available in the provided data\n", + "\n", + "# 2.2 Define conversion functions for each variable\n", + "\n", + "def convert_trait(value: str) -> int:\n", + " \"\"\"Convert trait values to binary format (0: Healthy, 1: Psoriasis).\"\"\"\n", + " if not isinstance(value, str):\n", + " return None\n", + " \n", + " value = value.lower().strip()\n", + " if \":\" in value:\n", + " value = value.split(\":\", 1)[1].strip()\n", + " \n", + " if value == \"psoriasis\":\n", + " return 1\n", + " elif value == \"healthy\":\n", + " return 0\n", + " return None\n", + "\n", + "def convert_age(value: str) -> Optional[float]:\n", + " \"\"\"Convert age values to continuous format.\"\"\"\n", + " # Age information is unavailable, but including function for completeness\n", + " return None\n", + "\n", + "def convert_gender(value: str) -> Optional[int]:\n", + " \"\"\"Convert gender values to binary format (0: Female, 1: Male).\"\"\"\n", + " # Gender information is unavailable, but including function for completeness\n", + " return None\n", + "\n", + "# 3. Save metadata about cohort usability\n", + "is_trait_available = trait_row is not None\n", + "validate_and_save_cohort_info(\n", + " is_final=False,\n", + " cohort=cohort,\n", + " info_path=json_path,\n", + " is_gene_available=is_gene_available,\n", + " is_trait_available=is_trait_available\n", + ")\n", + "\n", + "# 4. Clinical Feature Extraction (if trait data is available)\n", + "if trait_row is not None:\n", + " os.makedirs(os.path.dirname(out_clinical_data_file), exist_ok=True)\n", + " \n", + " # Sample characteristics dictionary needs to be loaded from input data\n", + " # Assuming clinical_data is already available in the environment\n", + " # If not, we need to first load it from a suitable source\n", + " \n", + " # Extract and process clinical features\n", + " selected_clinical_df = geo_select_clinical_features(\n", + " clinical_df=clinical_data,\n", + " trait=trait,\n", + " trait_row=trait_row,\n", + " convert_trait=convert_trait,\n", + " age_row=age_row,\n", + " convert_age=convert_age,\n", + " gender_row=gender_row,\n", + " convert_gender=convert_gender\n", + " )\n", + " \n", + " # Display a preview of the clinical data\n", + " print(\"Preview of extracted clinical data:\")\n", + " preview = preview_df(selected_clinical_df)\n", + " print(preview)\n", + " \n", + " # Save the clinical data to file\n", + " selected_clinical_df.to_csv(out_clinical_data_file)\n", + " print(f\"Clinical data saved to {out_clinical_data_file}\")\n" + ] + }, + { + "cell_type": "markdown", + "id": "0b7240f9", + "metadata": {}, + "source": [ + "### Step 3: Gene Data Extraction" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "448e7dc5", + "metadata": { + "execution": { + "iopub.execute_input": "2025-03-25T03:44:23.549381Z", + "iopub.status.busy": "2025-03-25T03:44:23.549258Z", + "iopub.status.idle": "2025-03-25T03:44:23.786822Z", + "shell.execute_reply": "2025-03-25T03:44:23.786427Z" + } + }, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Matrix file size: 394363 bytes\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "\n", + "Lines immediately after the marker:\n", + 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+ "!series_matrix_table_end\n", + "\n", + "\n", + "\n", + "\n", + "No gene data found after the marker.\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "No obvious gene identifiers found in the file.\n", + "\n", + "Checking SOFT file for gene data...\n", + "Line 1: !Database_name = Gene Expression Omnibus (GEO)\n", + "Line 11: !Series_summary = The study focuses on the cellular composition of the psoriasis epidermis, using single-cell transcriptomics to identify cell subsets and their interactions in both healthy and psoriatic skin. The research uncovers three keratinocyte populations and seven immune cell subsets exclusive to psoriatic lesions. A significant finding is the identification of a previously undetected population of plasmacytoid dendritic cells (pDCs) in the psoriatic epidermis, suggesting their role in the disease's pathogenesis. The study also highlights enhanced keratinocyte-immune cell interactions in psoriatic lesions, contributing to our understanding of psoriasis at the cellular level.\n", + "\n", + "Attempting to extract gene data from SOFT file...\n" + ] + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "\n", + "Failed to extract gene data from both matrix and SOFT files.\n", + "This dataset appears to have single-cell RNA-seq data which may not be in the standard GEO matrix format.\n", + "The dataset likely requires special parsing for single-cell data.\n" + ] + } + ], + "source": [ + "# 1. Re-identify the SOFT and matrix files to ensure we have the correct paths\n", + "soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir)\n", + "\n", + "# Print the file size to verify it's accessible\n", + "import os\n", + "file_size = os.path.getsize(matrix_file)\n", + "print(f\"Matrix file size: {file_size} bytes\")\n", + "\n", + "# Read a portion of the file after the marker to see the gene expression data structure\n", + "with gzip.open(matrix_file, 'rt') as f:\n", + " # Skip to the marker line\n", + " marker_line_found = False\n", + " for line in f:\n", + " if '!series_matrix_table_begin' in line:\n", + " marker_line_found = True\n", + " break\n", + " \n", + " if marker_line_found:\n", + " # Read the next few lines to see what the data looks like\n", + " print(\"\\nLines immediately after the marker:\")\n", + " for i in range(5): # Print 5 lines after the marker\n", + " line = f.readline().strip()\n", + " print(line)\n", + "\n", + "# Try a different approach to extract gene data - manually parse the file\n", + "gene_data = None\n", + "try:\n", + " with gzip.open(matrix_file, 'rt') as f:\n", + " # Skip to the start of the gene data\n", + " for line in f:\n", + " if '!series_matrix_table_begin' in line:\n", + " break\n", + " \n", + " # Read the header line\n", + " header = f.readline().strip().split('\\t')\n", + " \n", + " # Read the data lines\n", + " data_rows = []\n", + " indices = []\n", + " \n", + " for line in f:\n", + " if '!series_matrix_table_end' in line: # Stop at end marker if present\n", + " break\n", + " if line.strip(): # Skip empty lines\n", + " parts = line.strip().split('\\t')\n", + " if len(parts) > 1: # Ensure there's at least an ID and one value\n", + " indices.append(parts[0])\n", + " data_rows.append(parts[1:])\n", + " \n", + " if indices and data_rows:\n", + " # Create DataFrame\n", + " gene_data = pd.DataFrame(data_rows, index=indices, columns=header[1:])\n", + " print(f\"\\nManually extracted gene data with shape: {gene_data.shape}\")\n", + " else:\n", + " print(\"\\nNo gene data found after the marker.\")\n", + " \n", + " # Check if there are any genes at all in the file\n", + " f.seek(0) # Go back to beginning\n", + " gene_count = 0\n", + " for line in f:\n", + " if line.startswith('ENSG') or line.startswith('NM_') or line.startswith('XM_'):\n", + " gene_count += 1\n", + " if gene_count == 1:\n", + " print(f\"Sample gene line: {line.strip()}\")\n", + " if gene_count >= 5:\n", + " break\n", + " \n", + " if gene_count > 0:\n", + " print(f\"Found {gene_count} potential gene lines.\")\n", + " else:\n", + " print(\"No obvious gene identifiers found in the file.\")\n", + "except Exception as e:\n", + " print(f\"Error in manual parsing: {str(e)}\")\n", + "\n", + "# Inspect the SOFT file to see if it contains gene expression data\n", + "print(\"\\nChecking SOFT file for gene data...\")\n", + "with gzip.open(soft_file, 'rt', encoding='utf-8', errors='ignore') as f:\n", + " # Sample the first 100 lines to look for gene-related content\n", + " for i, line in enumerate(f):\n", + " if i < 100 and ('EXPR' in line or 'ID_REF' in line or line.startswith('ENSG') or 'gene' in line.lower()):\n", + " print(f\"Line {i}: {line.strip()}\")\n", + " if i >= 100:\n", + " break\n", + "\n", + "# Try reading gene expression data from the SOFT file\n", + "try:\n", + " print(\"\\nAttempting to extract gene data from SOFT file...\")\n", + " gene_expr_section = False\n", + " gene_data_lines = []\n", + " with gzip.open(soft_file, 'rt', encoding='utf-8', errors='ignore') as f:\n", + " for line in f:\n", + " if '!Sample_table_begin' in line:\n", + " gene_expr_section = True\n", + " # Get the header line\n", + " header_line = f.readline().strip()\n", + " gene_data_lines.append(header_line)\n", + " continue\n", + " if gene_expr_section and '!Sample_table_end' in line:\n", + " gene_expr_section = False\n", + " break\n", + " if gene_expr_section:\n", + " gene_data_lines.append(line.strip())\n", + " \n", + " if gene_data_lines:\n", + " # Create a DataFrame from the gene data lines\n", + " gene_data_str = '\\n'.join(gene_data_lines)\n", + " gene_data = pd.read_csv(io.StringIO(gene_data_str), delimiter='\\t', index_col=0)\n", + " print(f\"Successfully extracted gene data from SOFT file. Shape: {gene_data.shape}\")\n", + "except Exception as e:\n", + " print(f\"Error extracting gene data from SOFT file: {str(e)}\")\n", + "\n", + "# Print gene data info if available\n", + "if gene_data is not None and not gene_data.empty:\n", + " print(\"\\nFirst 20 gene/probe identifiers:\")\n", + " print(gene_data.index[:20].tolist())\n", + " print(f\"\\nGene data dimensions: {gene_data.shape[0]} genes × {gene_data.shape[1]} samples\")\n", + "else:\n", + " print(\"\\nFailed to extract gene data from both matrix and SOFT files.\")\n", + " print(\"This dataset appears to have single-cell RNA-seq data which may not be in the standard GEO matrix format.\")\n", + " print(\"The dataset likely requires special parsing for single-cell data.\")\n", + " \n", + " # Set is_gene_available to False since we couldn't extract the gene data in the expected format\n", + " is_gene_available = False" + ] + } + ], + "metadata": { + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.10.16" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +}