c06f438a_cture_20_Flashcards___Cram_com___the_genes_in_the_egg_or_sperm
[Germ Line Therapy] Mechanism of the PCR
We want to break the hydrogen bonds in DNA by raising the temperature The PCR will raise the temperature to 94 degrees for a minute or two and the strands will denature We now have specific single stranded DNA primers Lower the temperature to 45 – 65 degrees for 15 seconds so the primer can anneal to the strand with hydrogen bonding We then have DNA polymerase to extend the DNA in the 3’ direction at a temperature of 72 degrees
Semester Two: Biology Lecture 20 Flashcards - Cram.com