80aa785e__Universal___Life_Technologies__Possible_Solution
[Problem] PCR amplification is not observed. [Cause] This may indicate the presence of PCR inhibitors or that there are insufficient PCR cycles to achieve a product. [Possible Solution] 
If the former possibility is suspected: Ensure that the Washing Buffer is brought to room temperature. Add Washing Buffer more vigorously. Ensure that the supernatant is completely removed at each washing step. Introduce an additional (third) washing step. Use less starting material for the PCR. Titrate the amount of Mg2+, enzyme and dNTP used. If the latter possibility is suspected: Increase the number of PCR cycles.
Dynabeads® DNA DIRECT™ Universal | Life Technologies