7441fa2e_sis_system___Life_Technologies__Problem
[Reason] Reagents have lost activity [Solution] Store Expressway™ Expression Module reagents at –80°C. Store the T7 Enzyme Mix at –20°C after initial use. Use care when freezing and thawing the Expressway™ E. coli slyD–Extract, Expressway™ 2.5X IVPS E. coli Reaction Buffer, and Expressway™ 2X IVPS Feed Buffer. One or two freeze/thaw cycles are acceptable. Avoid multiple freeze/thaw cycles. Our long-term stability studies reveal MembraneMax™ Reagent to be very stable at a wide range of temperatures. [Problem] 
Control reactions produce no protein
MembraneMax™ Protein Expression Kits | for expression of recombinant membrane proteins in a cell-free protein synthesis system | Life Technologies