7441fa2e_sis_system___Life_Technologies__Problem
[Reason] DNA template not optimally configured [Solution] Use one of the recommended pEXP1-DEST, pEXP3-DEST, pEXP4-DEST, pEXP5-NT/TOPO®, or pEXP5-CT/TOPO® expression vectors or follow the guidelines at top of page to generate a DNA template with the optimal configuration. Make sure that the ATG initiation codon is in the proper context for expression (i.e., check spacing and placement after the RBS). Fusion of your protein to an N- or C-terminal tag may affect RNA structure and lower translation levels. Try moving the fusion tag to the other terminus. [Problem] 
Low or no yield of protein (but control reaction produces protein)
MembraneMax™ Protein Expression Kits | for expression of recombinant membrane proteins in a cell-free protein synthesis system | Life Technologies