7441fa2e_sis_system___Life_Technologies__Problem
[Reason] Improper protein folding [Solution] Reduce incubation temperature to as low as 25°C during protein synthesis. Membrane lipid composition is crucial for proper membrane protein folding. MembraneMax™ reagent may not contain the optimal lipid composition for the protein of interest [Problem] 
Protein has low biological activity
MembraneMax™ Protein Expression Kits | for expression of recombinant membrane proteins in a cell-free protein synthesis system | Life Technologies