6d98bf75_Academic_Course_List__Name
[ClassID] PHRM 5120 [Description] This course will expand on the basic concepts covered in CHEM4520. Students will deepen their knowledge of and handson experience with cloning, bacterial expression, enzyme purification and assay development. Students will perform a semesterlong project that involves amplification of a gene using PCR, cloning the PCR product, expressing the protein in E. coli, purifying the protein, and developing and optimizing an assay to measure enzyme activity. The purification will employ the AKTA System, a stateoftheart purification system from GE Healthcare that is specifically designed for protein purification. Handson practical experience will be gained in molecular biology, general biochemical techniques, and fundamental enzymology. Time 3 lab hours twice a week. Prerequisite CHEM 31203320 Organic Chemistry and lab, CHEM45004520 Biochemistry and Lab, or with the consent of the instructor [Credits] 2 [Name] 
Advanced Biochemistry Lab
Academic Course List