80aa785e__Universal___Life_Technologies__Possible_Solution
[Problem] The number of PCR reactions possible is lower than expected. [Cause] If fragmentation of the complex was observed during the washing steps, washing may have been too vigorous. If fragmentation was not observed, elution may have been inefficient. [Possible Solution] 
If the former possibility is suspected: Add Washing Buffer less vigorously. If the latter possibility is suspected: Run out some of the Dynabeads on an agarose gel. If DNA is found to be predominantly on the beads, then elution was inefficient. Ensure that all Washing Buffer is removed before adding Resuspension Buffer. Increase the force used to homogenize the complex. Increase sample quality and quantity. Increase the number of PCR cycles.
Dynabeads® DNA DIRECT™ Universal | Life Technologies