7441fa2e_sis_system___Life_Technologies__Problem
[Reason] Samples not precipitated with acetone [Solution] Precipitate the proteins with acetone to remove background smearing. Follow the protocol Generating the DNA Template. [Problem] 
Smearing on the gel
MembraneMax™ Protein Expression Kits | for expression of recombinant membrane proteins in a cell-free protein synthesis system | Life Technologies