7441fa2e_sis_system___Life_Technologies__Problem
[Reason] Sample incubated in a water bath or non-shaking incubator [Solution] Incubate sample in a thermomixer or incubator with shaking [Problem] 
Low or no yield of protein (but control reaction produces protein), continued
MembraneMax™ Protein Expression Kits | for expression of recombinant membrane proteins in a cell-free protein synthesis system | Life Technologies