Cytoplasmic dynein is a microtubule motor that mediates various biological processes, including nuclear migration and organelle transport, by moving on microtubules while associated with various cellular structures. The association of dynein with cellular structures and the activation of its motility are crucial steps in dynein-dependent processes. However, the mechanisms involved remain largely unknown. In fungi, dynein is required for nuclear migration. In budding yeast, nuclear migration is driven by the interaction of astral microtubules with the cell cortex; the interaction is mediated by dynein that is probably associated with the cortex. Recent studies suggest that budding yeast dynein is first recruited to microtubules, then delivered to the cortex by microtubules and finally activated by association with the cortex. Nuclear migration in many other fungi is probably driven by a similar mechanism. Recruitment of dynein to microtubules and its subsequent activation upon association with cellular structures are perhaps common to many dynein-dependent eukaryotic processes, including organelle transport.