Apart from the beneficial roles of pyrogallol in industries, it also tends to produce free radicals that trigger apoptosis in human cells. In this study, we checked the toxic effect of pyrogallol in fission yeast  S. pombe  cells. We observed that the wild type and  wat1/pop3  delete cells were unable to grow on plates containing pyrogallol in a dose-dependent manner. Furthermore, the  wat1/pop3  delete cells exhibit higher sensitivity against pyrogallol as compared to wild type cells suggesting that the pyrogallol induces oxidative stress. The exposure to pyrogallol also leads to the production of ROS and affects the sporulation in  S. pombe. 