 Schizosaccharomyces pombe  is a model unicellular eukaryote with ties to the basic research, oenology and industrial biotechnology sectors. While most investigations into  S. pombe  cell biology utilize Leupold's 972 h   -  laboratory strain background, recent studies have described a wealth of genetic and phenotypic diversity within wild populations of  S. pombe  including stress resistance phenotypes which may be of interest to industry. Here we describe the genomic and transcriptomic characterization of Wilmar-P, an  S. pombe  isolate used for bioethanol production from sugarcane molasses at industrial scale. Novel sequences present in Wilmar-P but not in the laboratory  S. pombe  genome included multiple coding sequences with near-perfect nucleotide identity to  Schizosaccharomyces octosporus  sequences. Wilmar-P also contained a ∼100kb duplication in the right arm of chromosome III, a region harboring  ght5   + , the predominant hexose transporter encoding gene. Transcriptomic analysis of Wilmar-P grown in molasses revealed strong downregulation of core environmental stress response genes and upregulation of hexose transporters and drug efflux pumps compared to laboratory  S. pombe  Finally, examination of the regulatory network of Scr1, which is involved in the regulation of several genes differentially expressed on molasses, revealed expanded binding of this transcription factor in Wilmar-P compared to laboratory  S. pombe  in the molasses condition. Together our results point to both genomic plasticity and transcriptomic adaptation as mechanisms driving phenotypic adaptation of Wilmar-P to the molasses environment and therefore adds to our understanding of genetic diversity within industrial fission yeast strains and the capacity of this strain for commercial scale bioethanol production.