The present investigation was conducted to study the genotoxic effects of waste oil of frying bean cake (Taamiah oil) using Saccharomyces cerevisiae and Schizosaccharomyces pombe as test organisms. The results showed that the different concentrations of Taamiah oil exert different toxicity on yeast cells. the induced toxicity in both organisms was gradually increased with rising the concentration of Taamiah oil While, the differences between cellular survival and respiratory deficient mutants in treated and untreated samples were significant. Though Taamiah oil induced a concentration-dependent toxicity, it did not exert an induction of recombination. Thus, it seems likely that there was a cytotoxic effect and a weak effect on the induction of cytoplasmic petite mutations in yeast. The results suggest that Taamiah oil does not seem to induce lesions in DNA that are subject to excision repair. However, in Schizosaccharomyces pombe some point mutations seem to be induced in addition to toxicity. The conclusion is straight forward that waste oil of frying bean cake is mutagenic and may be carcinogenic in humans.