This protocol describes the live observation of chromosome segregation during fission yeast meiosis. To visualize one chromosome of interest, the  lac  operator ( lacO  array) is integrated at its centromere-proximal locus, and the  lac  repressor (lacI)-GFP fusion protein is expressed in a haploid strain. This haploid strain, in which mCherry-tagged tubulin is also expressed exogenously to monitor meiotic progression, is crossed with a nonlabeled haploid strain to induce meiosis. GFP and mCherry signals in resulting zygotes are observed by a fluorescent microscopy during the progression of meiosis.