Funding opportunities   Funding Type:  Tools and Technologies II
Grant Number:  Funds requested:  $1 593 036 Funding
Recommendations:  Not recommended Grant approved:  Public
Abstract:  Statement of Benefit to California:  Review Summary: 
The goal of this application is to develop a non-genetic, label-
free technology to sort cells using their responses to
electrical stimulation, in order to achieve sufficient purity
for cardiac cell replacement therapies. First, the applicant
proposes to engineer a microfluidic cell sorter and associated
instrumentation for separating individual cells based on their
electrical excitability. Next, the applicant will demonstrate
cell sorting of single induced pluripotent stem cell-derived
cardiomyocytes (iPSC-CMs), and confirm their cardiac myocyte
subtypes using standard assays. Finally, the applicant will
scale up the cell sorting technology to achieve throughputs in
the range of 1 million cells/hour. Reviewers agreed that
heterogeneity of cell types in iPSC-CM preparations