Email updates   Open Access Highly Accessed Research    Author
Affiliations    For all author emails, please log on.
Received:2 December 2005 Revisions received:21 April 2006
Accepted:19 May 2006 Published:19 June 2006  © 2006 de Godoy et
al.; licensee BioMed Central Ltd.           Technological goals
of proteomics include the identification and quantification of
as many proteins as possible in the proteome to be investigated
[1-3]. However, despite spectacular advances in mass
spectrometric technology, no cellular or microorganismal
proteome has been completely sequenced yet. This has not
hindered successful application of proteomics, as most
biologically relevant studies have focused on functionally
relevant 'subproteomes'. For example, our laboratory has been
interested in protein constituents of organelles such as the
nucleolus and mitochondria [4-6]. These proteomes have
complexities of about a 1,000 proteins and are largely within
reach of current technology. Other fruitful areas of proteomics
have b