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updates. Gene Splicing by Overlap Extension: Tailor-Made Genes
Using the Polymerase Chain Reaction Robert M. Horton, Zeling
Cai, Steffan N. Ho, and Larry R. Pease Mayo Clinic
BioTechniques, Vol. 54, No. 3, March 2013, pp. 129–133 Full Text
(PDF)  Gene Splicing by Overlap Extension or “gene SOEing” is a
PCR-based method of recombining DNA sequences without reliance
on restriction sites and of directly generating mutated DNA
fragments in vitro. By modifying the sequences incorporated into
the 5′-ends of the primers, any pair of polymerase chain
reaction products can be made to share a common sequence at one
end. Under polymerase chain reaction conditions, the common
sequence allows strands from two different fragments to
hybridize to one another, forming an overlap. Extension of this
overlap by DNA polymerase yields a recombinant molecule. This
powerful and technically simple approach offers many advantages
over conventional approaches for manipulatin