, Volume 25, Issue 5, pp 445-450  Variations in ploidy among
isolates of Botrytis cinerea: implications for genetic and
molecular analyses  Rent the article at a discount  Rent now  *
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Field isolates and laboratory strains of Botrytis cinerea, an
ascomycetous fungus causing considerable economic losses, e.g.,
as “grey mould” of vine, were compared for differences in ploidy
level by determining their DNA content per nucleus. Strain
SAS56, an ascospore line used routinely for genetic analyses, is
probably polyploid, since treatment with benomyl causes a
significant reduction in DNA content per nucleus. This
conclusion is substantiated by the increased sensitivity of the
putative haploid derivatives to mutagens (UV and EMS). Molecular
analyses (RAPD) of the haploidized strains indicate a very
limited degree of heterozygosis of the parent strain SAS56.
Analysis of field isolates of B. cinerea showed that their DNA
content per nucleus varied consider