Sucrose as a cryoprotectant  Hi, all histonetters  What is the
rationale for using sucrose as a cryoprotectant. For cryostat
sectioning of rat brain we use graded concentrations of sucrose
(15%--> 20--> 30%) for cryoprotection. What is the advantage of
graded concentrations as opposed to immersing the tissue
directly in sucrose. Hope it merits an answer.  sunil thomas K
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