  DVDY_22383_sm_suppinfoFigS1.tif2028KSupp. Fig. 1. Knock-down
of atp6v0c2 in zebrafish embryos. A: Schematic diagrams of
atp6v0c2 genomic DNA and splice sites targeted by E2I2 MOs
(atp6v0c2 MO). atp6v0c2 MOs block the excision of the intron
between exon 2 and exon 3, generating abnormal atp6v0c2 mRNA in
which exon 2 and exon 3 are separated by the second intron (I2).
B: Confirmation of the efficiency of atp6v0c2 MOs by reverse
transcriptase-PCR (RT-PCR) analysis. RT-PCR with RNA from
control embryos amplifies a 677-bp segment of the transcript.
However, RT-PCR using RNA from atp6v0c2 MO-injected embryos
failed to generate a PCR product, because excision of the
2,169-bp intron (I2) between exon 2 and exon 3 did not occur. C:
Confirmation of the efficiency of atp6v0c2 MOs by in situ
hybridization with atp6v0c2 RNA probe. All panels show lateral
views of wild-type and atp6v0c2 MO-injected embryos analyzed by
atp6v0c2. Since atp6v0c2 MOs interfere with splicing between
exon2 and exon3 and generate an mRNA split