Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)
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Sack (Talk | contribs) Jump to: navigation, search  Revised by
Kenneth Eum on 11-15-2011  Cell Media with Selection Agents
(Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line
F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000
Blasticidin, 1/4,000 Zeocin  Blasticidin = 10mg/ml stock Zeocin
= 100mg/ml stock    1. Obtain a 50mL conical tube and label as:
• “Ham’s F-12 Media + 10% FBS + 1% Pen/Strep + 1ug/ml blast +
25ug/ml zeo”     • Include date made and initials   2. Add
44.5mL of Ham’s F-12 Media to the labeled 50mL conical tube   3.
Add 5mL of FBS   4. Add 500µL of Pen Strep   5. Add 5µl of
blasticidin (Final Concentration (1 µg/ml))   6. Add 12.5µl of
zeocin (Final Concentration (25 µg/ml))   7. Close the conical
tube tightly and store in 4°C  Personal tools