Top image  Jechlinger Group  Mechanisms of oncogene dependence
and tumour relapse   (A) One cell in anaphase divides with the
sets of chromosomes perpendicular to the apical surface, while
keeping ZO1 localised to the luminal membrane.  (B) Confocal
microscopy (5mm projection through the middle) shows epithelial
cell polarity. E-Cadherin, (adherens junctions, lateral); ZO1,
(tight junctions, apical); Integrin a6 (basolateral).  (C)
Doxycycline exposure causes loss of epithelial polarity and
filling of the lumen; Removal of doxycycline results in survival
of a re-polarised cell layer, that acquires the ability to
exclude Hoechst 33342. Left panels: Confocal microscopy (5mm
projection through the middle) shows Dapi, GM130 (apical), ZO1
(tight junction), Integrina6 (basal) at indicated times. Middle
panels: Bright-field pictures show: (top) small, hollow acini;
(middle) filled, irregular shaped spheres (bottom) hollow,
irregular shaped spheres that show debris of internal cells.
Right panels: Exclusion of Hoechs