GUS irregular expression pattern,,????????  이인철 ichoki at Wed
Mar 27 20:00:47 EST 2002    I have a problem,,,   If you know,
teaches to me,,,, Its My major jobs..  My T-DNA is fussioned
pCAMBIA3300(contain BAR) and pBI121(contain  GUS)---->pCAMBIA
backbone and Transforamtion to the columbia. Then sowing basta
selected T2  line.( sowing five allele for each lines)  21 days
3'-mature green leaf sampling(GL) 35 ~ 40 days 20% 4'-yellow
leaf sampling(YL)  ??? YL gus staining line is many(12%~16%),
YL line number is    more than GL line number,    and many lines
YL gus color is more deep blue then ML gus color.   and major
problem is contain the partial spot- irregular type in same
line.  I do't know. Why happened these  types..  I guess some
effect factors.    1.problem my sampling technic.
2.environmental effect in stress.(anthocyanin accmulation)
3.pCAMBIA backbone problem.    4.some artificial effect.  Dear
researchers. Good Luck to you..*^^*  More information about the
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