Griffin:shRNA Transfection  From OpenWetWare  Revision as of
14:13, 17 February 2011 by Korey Griffin (Talk | contribs) Jump
to: navigation, search   Lentivirus, shRNA plasmid, or siRNA?
Choosing between Lentivirus, shRNA transfer vector, or siRNA,
depends on what you are seeking to accomplish. What advantage is
there for you to create a stable knockdown versus a transient
knockdown? If you are running your RNAi on an easy to transfect
cell line, under a transient RNAi event, then siRNA is a well
defined, proven and effective method. If you are working with a
primary cell, neuron, or generally hard to transfect cell, then
lentiviral particles for introducing shRNA is attractive. The
shRNA transfer vector alone in theory should be easy to work
with insofar as culturing the cell, DNA transfection, antibiotic
selection, and then collect data. However the true purpose of
the lentivirus transfer vector is for packaging into lenti
particles.  Establishing a stable knockdown phenotype is
feasible with the use of le