Display Settings:   Send to:  Choose Destination J Biol Chem.
1999 Dec 17;274(51):36456-64.  Molecular cloning of a zinc
finger autoantigen transiently associated with interphase
nucleolus and mitotic centromeres and midbodies. Orthologous
proteins with nine CXXC motifs highly conserved from nematodes
to humans.   Departamento de Bioquímica y Biología Molecular,
Facultad de Ciencias, Universidad de Cádiz, 11510 Puerto Real,
Cádiz, Spain.   We have cloned a novel human autoimmune antigen
in a patient suffering from rheumatoid arthritis with high
levels of antibodies to the nucleolus organizer regions.
Initially the human autoimmune serum was used to select a cDNA
of 317 amino acids from a hamster expression library. Using the
hamster DNA as a probe, we isolated the human homologous cDNA of
320 amino acids. Human and hamster polypeptides share a 95%
amino acid homology. The deduced 36-kDa protein contains a
putative amino-terminal NLS signal, nine cysteine-X-X-cysteine
motifs highly conserved, and a carboxyl-te