Chapter 12: DNA Technology Untitled Document  Biology and
Society: Hunting for Genes 1. Define DNA technology and describe
advances using these methods.  2. List four ways that DNA
technology plays a significant role in our lives.  Recombinant
DNA Technology 3. Describe the advances made over the last three
decades using DNA technology.  4. Explain how genetic
engineering is used to produce humulin, human growth hormone,
vaccines, crops, and "pharm" animals.  5. Explain how
recombinant DNA techniques are used to mass produce a protein
from an isolated gene.  6. Describe the process of cloning genes
using plasmids.  7. Explain how restriction enzymes and DNA
ligase are used to create recombinant DNA.  8. Explain how the
“shotgun” approach produces a genomic library.  DNA
Fingerprinting and Forensic Science 9. Describe the many ways
that DNA fingerprinting can be used.  10. Explain how the
polymerase chain reaction (PCR), restriction fragment length
polymorphism (RFLP) analysis, and gel electrophoresis are used