Logo of narLink to Publisher's site Nucleic Acids Res. 2011 Jul
1; 39(Web Server issue): W551–W556. Published online 2011 May
11. doi:  10.1093/nar/gkr312 PMCID: PMC3125748    Bisulfite
sequencing is a widely used method for measuring DNA methylation
in eukaryotic genomes. The assay provides single-base pair
resolution and, given sufficient sequencing depth, its
quantitative accuracy is excellent. High-throughput sequencing
of bisulfite-converted DNA can be applied either genome wide or
targeted to a defined set of genomic loci (e.g. using locus-
specific PCR primers or DNA capture probes). Here, we describe
BiQ Analyzer HT (http://biq-analyzer-ht.bioinf.mpi-inf.mpg.de/),
a user-friendly software tool that supports locus-specific
analysis and visualization of high-throughput bisulfite
sequencing data. The software facilitates the shift from time-
consuming clonal bisulfite sequencing to the more quantitative
and cost-efficient use of high-throughput sequencing for
studying locus-specific DNA methylation pattern