Real-time polymerase chain reaction  From Wikipedia, the free
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navigation, search For reverse transcription polymerase chain
reaction (RT-PCR), see reverse transcription polymerase chain
reaction. SYBR Green fluorescence chart produced in real-time
PCR. Melting curve prudced at the end of real-time PCR.  A real-
time polymerase chain reaction is a laboratory technique of
molecular biology based on the polymerase chain reaction (PCR).
It monitors the amplification of a targeted DNA molecule during
the PCR, i.e. in real-time, and not at its end, as in
conventional PCR. Real-time PCR can be used quantitatively
(Quantitative real-time PCR), semi-quantitatively, i.e.
above/below a certain amount of DNA molecules (Semi quantitative
real-time PCR) or qualitatively (Qualitative real-time PCR).
Two common methods for the detection of PCR products in real-
time PCR are: (1) non-specific fluorescent dyes that intercalate
with any double-stranded DNA, and (2) sequence