Document Detail  Nuclear expression of E2F4 induces cell death
via multiple pathways in normal human intestinal epithelial
crypt cells but not in colon cancer cells. MedLine Citation:
PMID:  17656449     Owner:  NLM     Status:  MEDLINE     E2F
transcription factors control cell cycle progression. The
localization of E2F4 in intestinal epithelial cells is cell
cycle dependent, being cytoplasmic in quiescent differentiated
cells but nuclear in proliferative cells. However, whether
nuclear translocation of E2F4 alone is sufficient to trigger
intestinal epithelial cell proliferation remains to be
established. Adenoviruses expressing fusion proteins between
green fluorescent protein (GFP) and wild-type (wt)E2F4 or GFP
and nuclear localization signal (NLS)-tagged E2F4 were used to
infect normal human intestinal epithelial crypt cells (HIEC). In
contrast to expression of wtE2F4, persistent expression of E2F4
into the nucleus of HIEC triggered phosphatidylserine exposure,
cytoplasmic shrinkage, zeiosis, formation of