A-MEXP-313 - SPC Pseudomonas putida genome array 5K  Pseudomonas
putida The P. putida KT2440 DNA microarray developed contains
5539 oligonucleotides (50-mer) spotted in duplicate onto
?-aminosylane treated 25x75 mm microscope slides, covalently
linked to the slide with UV light and heat. The spots, 180 ?m in
diameter, are separated from each other by 265 ?m and arranged
in 48 subarrays (16 columns and 15 rows each). The spotted area
is ?17x54 mm. The oligonucleotides, designed by BioAlma
(www.bioalma.com), are specific for each ORF in the array. These
include the 5350 ORFs annotated in the P. putida KT2440 genome
(obtained from www.ncbi.nlm.nih.gov, Refseq NC_002947; GenBank
accession number AE015451.1), the 140 ORFs defined for the TOL
plasmid pWW0 (obtained from www.ncbi.nlm.nih.gov, Refseq
NC_003350; GenBank accession number AJ344068.1), and other genes
of diverse origins such as commonly used reporter genes (lacZ,
gfp) or antibiotic resistance markers. Two oligonucleotides,
coding for the rpoD and rpoN ge