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Protocols  Colony picking using cloning rings  There are several
ways to obtain clonal populations after you stably transfect a
cell line (using different approaches).  The way I do it
involves using cloning rings (chemicon and others sell them w/
grease already, but you can just get regular cylinders and use
your own grease instead) and it's very straight-forward. I'm
using chemicon's TR-1004.    1. After you have transfected and
initiated selection for some days (depends on cell line
efficiency, etc), take out the media   2. Wash once with PBS and
aspirate it   3. Remove plate cover and tilt the plate towards
you at an angle at which you can clearly see the surface of the
plate with irregularities, such as, a new colony! This means you
won't be picking colonies that are really really small. After
you do it for a while, you'll get the hang of it. If you are
doing the selection on small dishe