Figure 2.  Heterologously expressed GPCRs are integrated into an
endogenous signalling pathway in C. elegans. Mutation of the
TRPV channel subunit osm-9 and the Gα subunit gpa-3 fully
abolished avoidance of (A) 25 μM SST-28 in Sstr2 transgenic
animals and (B) 25 μM MIP-1α in CCR5 transgenic animals.
Mutation of Gα subunit odr-3 did not affect these responses. (C)
In contrast, mutation of either gpa-3 or odr-3 did not have a
significant effect on avoidance response in sra6::CCR5
transgenic animals. Each data point represents an average of at
least 3 independent assays for wild type and each respective
strain. Error bars denote the standard error of the mean.
Asterisks indicate a statistically significant difference
between osm-9 or gpa-3 mutant animals and wild type animals
carrying the Sstr2 or CCR5 transgenes (*p ≤ 0.05, **p ≤ 0.01,
***p ≤ 0.005).  Teng et al. BMC Biology 2006 4:22  
doi:10.1186/1741-7007-4-22 Download authors' original image