SGD Paper Help  Mayor T, et al.  (2005) Analysis of
polyubiquitin conjugates reveals that the Rpn10 substrate
receptor contributes to the turnover of multiple proteasome
targets. Mol Cell Proteomics 4(6):741-51  Abstract: The poly-
ubiquitin receptor Rpn10 targets ubiquitylated Sic1 to the 26S
proteasome for degradation. In contrast, turnover of at least
one ubiquitin-proteasome system (UPS) substrate, CPY*, is
impervious to deletion of RPN10. To distinguish whether RPN10 is
involved in the turnover of only a small set of cell cycle
regulators that includes Sic1, or plays a more general role in
the UPS, we sought to develop a general method that would allow
us to survey the spectrum of ubiquitylated proteins that
selectively accumulate in rpn10 cells. Poly-ubiquitin conjugates
from yeast cells that express hexahistidine-tagged ubiquitin
(H6-ubiquitin) were first enriched on a poly-ubiquitin binding
protein affinity resin. This material was then denatured and
subjected to immobilized metal ion affinity chromato