Quenching peroxidase activity??!!??  << Previous Message | Next
Message >> From:"John C. Dennis" <dennijc@vetmed.auburn.edu> (by
way of histonet) To:histonet <histonet@magicnet.net>  dear folks
After the flurry of discussion on quenching endogenous
peroxidase-like activity, I tried one of the protocols.  It was
the one using 3% peroxide in PBS.  This one is also called for
in the ONCOR Apotag Kit.  I don't recommend following the
instructions in this detail if you are looking at apoptosis in
rat brain.  My tissue is rat olfactory and VNO epithelium
transplanted to rat brain. With a five minute bath in the above
peroxide solution, I had plenty of DAB(+) cells in my secondary
control (i.e. no primary).  With a five minute bath in 3%
peroxide/absolute MetOH, the control slide was clean.  I grant
that, at the same primary dilution, the signal was attenuated
but still acceptable given the dramatic decrease in background.
By the by, the background was not a generalized brown but infact
subpopulations of cells tha