 About Cache Protocols Used at NCI and Related Information:
Slide Preparation for Manual Microdissection  Slide Preparation
for Manual Microdissection (for Subsequent DNA, RNA, and Protein
Analysis)  These methods were successful in our lab using
prostate tissue and for our specific objectives. Investigators
must be aware that they will need to tailor the following
protocol for their own research objectives and tissue under
study.  Manual microdissection and subsequent molecular analysis
can be carried out on slides stained using standard hematoxylin
and eosin methods. However, if cell types that are (or are not)
expressing a specific protein are required for a study, then
more advanced slide preparation methods such as Immuno-LCM may
be utilized.  1. Materials      1. 70%, 95%, 100% ethanol     2.
Xylenes, mixed, ACS reagent (Sigma)     3. Glycerol, ultra pure
(Gibco)     4. Deionized water     5. Hematoxylin solution,
Mayer's (Sigma)     6. Eosin Y solution (Sigma)     7. Complete,
mini protease inhibitor c