SGD Paper Help  Bode HP, et al.  (1995) Iron sequestration by
the yeast vacuole. A study with vacuolar mutants of
Saccharomyces cerevisiae. Eur J Biochem 228(2):337-42  Abstract:
To investigate iron uptake by the yeast vacuole, we examined
iron accumulation and subcellular distribution in two
Saccharomyces cerevisiae mutant strains with distinct
alterations in vacuolar morpholgy and functions, the vacuolar
protein targeting mutants vpt13 and vpt16. The mutant vpt13 is
deficient in vacuolar acidification but possesses a
morphologically normal vacuole, whereas vpt16 is devoid of any
vacuole-like structure. The vacuole-lacking mutant vpt16
displayed reduced iron accumulation at high and low
extracellular iron concentrations. Iron accumulation by vpt13
was not reduced at low extracellular iron concentrations, and
only slightly reduced at high external iron. Subcellular
fractionation of vpt13 after iron loading confirmed the vacuolar
localization of the accumulated iron. Vacuolar iron
sequestration thus is not dep