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Clipboard (0)  Related Articles  1.  Discovery of chemically
induced mutations in rice by TILLING  BMC Plant Biology 
2007;7:19. Rice is both a food source for a majority of the
world's population and an important model system. Available
functional genomics resources include targeted insertion
mutagenesis and transgenic tools. While these can be powerful, a
non-transgenic, unbiased targeted mutagenesis method that can
generate a range of allele types would add considerably to the
analysis of the rice genome. TILLING (Targeting Induced Local
Lesions in Genomes), a general reverse genetic technique that
combines traditional mutagenesis with high throughput methods
for mutation discovery, is such a method. We estimate that the
density of induced mutations is two- to threefold higher than
previously reported rice populations (about 1/300 kb). By
comparison to other plants used in public TILLING services, we
conclude that the populations described here would be s