Figure 8.  CD200 expression on apoptotic cells affected their
binding and phagocytosis by dendritic cells. (A) Immature
dendritic cells (DCs) (stained with PKH67, green) were incubated
with target cells pre-labeled with PKH26 (red) and examined
under immunofluorescence microscopy. White arrowheads, target
cells attached to immature DCs; yellow arrowheads, target cells
engulfed by immature DCs. Upper left, CD200- live cells; upper
right, CD200+ live cells; lower left, CD200- apoptotic cells;
lower right, CD200+ apoptotic cells. (B) Quantification of the
percentages of DCs that bound and ingested target cells by
immunofluorescence microscopy. The percentages were calculated
by counting all the cells in 10 microscope fields (average 40 to
50 cells each microscope field and 400 to 500 cells were
recorded in total).  Download authors' original image