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Multiplex PCR Analysis  Combining Specificity and Sensitivity
with High Performance for a Multitude of Applications    • Click
Image To Enlarge +      Multiplex PCR is a difficult technique
that allows amplification of two or more products in parallel in
a single reaction tube. This technology was first described by
Chamberlain et al., in 1988, and has since been applied in
diverse research areas, including analyses of deletions, SNPs,
mutations, microsatellites, polymorphisms, pathogen
identification, linkage analysis, gene expression, and forensic
studies (Table).      Multiplex PCR, if sensitive enough, can be
used to amplify and detect a single copy of a nucleic acid
sequence and may also be used for both end-point and real-time
PCR applications.      Multiplex PCR ensures standardization in
certain experiments because identical reaction conditions and
template amounts are used, pipetting and cycling condition
variations are eliminated, and rel