NLM AIDSLINE Immediate effects of reversible HTLV-1 tax
function: T-cell activation and apoptosis. Chlichlia K;
Moldenhauer G; Daniel PT; Busslinger M; Gazzolo L; May 30, 1995
Oncogene. 1995 Jan 19;10(2):269-77. Unique Identifier : AIDSLINE
The tax protein of Human T-cell leukemia virus type 1 (HTLV-1)
is important for the transforming properties of this virus in
vitro and is considered to be responsible for the early stages
of leukemogenesis in infected hosts. To address the early
consequences of HTLV-1 tax function, we have constructed fusion
proteins containing tax sequence either aminoterminal (taxER) or
carboxy-terminal (ERtax) of the hormone binding domain of the
human estrogen receptor (ER). Addition of estrogen or the
antagonist hydroxytamoxifen to Jurkat T-cells expressing these
constructs led to the trans-activation or responsive promoters
and upregulation of cell surface markers CD28, CD69 and CD5 but
not CD25 (IL2R-alpha subunit) or B7 (ligand for CD28).
Additional stimulation of the T-cell recept