User:Mackenzie L. Cowell/IGEM 2007 part fab protocol  From
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search (96d glycerols) (384d antibiotic test) Line 114: Line
114: We can prepare all the AB plates in advance with the EPM. *
howto: make 16 AB test plates * Use 4 methods, one for each
antibiotic. * 100ul EZ Rich broth with appropriate antibiotic in
each well.  100ul * 96 wells = 9600ul of each antibiotic broth
per plate * 8 plates per run = 76,800ul of broth per run (20ml
per reservoir * 4 reservoirs) ==Typical concentrations and
volumes== ==Typical concentrations and volumes==  Revision as of
10:00, 18 April 2007      Wed 04.18.07    1. Prepare 8 96d
miniprep plates     1. just use 2mL LB-amp per well (1536 mL
total LB-amp)   2. Prepare 16 AB plates (one for each unique
library plate)   3. Thaw library plates 1-4 (96d glycerol
stocks)   4. Inoculate the miniprep and AB plates,     1. if the
pen-tool is available       1. use it to inoculate 2 miniprep
plates for each library plate