Real-time polymerase chain reaction  From Wikipedia, the free
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navigation, search SYBR Green fluorescence chart for five
samples, each having three replicates, which is a result of
quantitative PCR (qPCR). Melting curve for five samples, three
replicates each, which is a result of melting temperature
analysis of quantitative PCR results (qPCR).   The procedure
follows the general principle of polymerase chain reaction; its
key feature is that the amplified DNA is detected as the
reaction progresses in "real time". This is a new approach
compared to standard PCR, where the product of the reaction is
detected at its end. Two common methods for the detection of
products in quantitative PCR are: (1) non-specific fluorescent
dyes that intercalate with any double-stranded DNA, and (2)
sequence-specific DNA probes consisting of oligonucleotides that
are labelled with a fluorescent reporter which permits detection
only after hybridization of the probe with its co