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Determining low product cut off using Ct - How to decide Ct
level at which expression is unreliable (Mar/25/2007 )   I am
using SYBR green for relative quanification, and analysing my
first real-time data. The expression levels of my target gene
are quite low or no expression in some tissues that I am looking
at.  I was wondering if anyone knew of a method (justified and
preferably published) for creating a cut off point for when your
Ct value gets beyond 37 and when some replicates (in a
triplicate) are undetectable.  Hope this makes sense. I can try
to explain further if needed.     I actually have the same
question. One replicate in a triplicate often often has Ct value
of "N/A". How does one quantify that in calculations?