• My NEB   • Print   • PDF   • SFP Synthase       SFP Synthase
(4´-phosphopantetheinyl transferase) catalyzes the covalent
transfer of substituents from derivatized coenzyme A (CoA) to
ACP- or MCP-tagged fusion proteins exposed on the surface of
living cells. The 25 nmoles of SFP Synthase provided is
sufficient to make 25 ml of a 1 µM ACP-tag or MCP-tag fusion
protein labeling solution.      The ACP-tag and MCP-tag are
small protein tags (8 kDa) based on the acyl carrier protein.
MCP-tag contains two mutations (D36-T36 and D39-G39). Both allow
the specific, covalent attachment of virtually any molecule to a
protein of interest. Substrates for labeling are derivatives of
coenzyme A (CoA). In the labeling reaction, the substituted
phosphopantetheine group of CoA is covalently attached to a
conserved serine residue on the ACP-tag or the MCP-tag by a
phosphopantetheine transferase (SFP Synthase or ACP Synthase).
Having no cysteines, the ACP-tag and the MCP-tag are
particularly suited for specifically labeling cel