You are here  How can I calculate pixel intensity in an ROI?
I'm looking for suggestions on how I can use GIMP to calculate
pixel intensity in an ROI. This kind of function is frequently
used in gel electrophoresis analysis, where the software will
identify gel bands and calculate relative concentration based on
pixel intensity. I'm actually analyzing microscope images, but
the principle is the same. I need to calculate the average
intensity of pixels within a specific region. Any suggestions?
I would also be very interested in what you mentioned. Would be
glad, if someone has an idea. greets, Benjamin  What I could
think about a stupid way to do it is by means of histogram, as
follows: 1. Highlight a blank lane of gel or background and
check the limits of pixels in the histogram, say 170-255. The
smaller figure will be used later. 2. Move the same rectangular
mask to a lane containing one band. Redo the histogram. This
time you should see more pixels distributing on the left side of
the histogram, i.e. sma