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Biochemistry  phage display - peptide differentiation
(Jul/27/2010 )  I don't know if this is the right forum, and in
case it isn't sorry and please direct me to the right one...
I'm working with phage display libraries from NEB. I want to
find a non destructive way of differentiation between/grouping
peptides from a sample. Each phage has between 3-5 identical
peptides displayed on its coat protein. Each sample (0,5-1 ml of
eluted phage) contains 10^2-10^4 (estimate) phage particles,
where the phage part is the same, only difference is the
peptide. There could be up to ~50 clones of each peptides-
specific phage. Any ideas as how to do this?   Hi Chaim,  I am
guessing that you got your information already, but anyway.
There is absolutely no way to do this. The whole point of
biopanning is to enrich for clones that have higher affinities
for the target than phage you wash away. With the libraries you
are using there are usually closer to 10^7 pfu/ml of