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NSOM/QD-Based Visualization of GM1 Serving as Platforms for
TCR/CD3 Mediated T-Cell Activation  BioMed Research
International  2013;2013:276498. Direct molecular imaging of
nanoscale relationship between T-cell receptor complexes
(TCR/CD3) and gangliosidosis GM1 before and after T-cell
activation has not been reported. In this study, we made use of
our expertise of near-field scanning optical
microscopy(NSOM)/immune-labeling quantum dots- (QD-)based dual-
color imaging system to visualize nanoscale profiles for
distribution and organization of TCR/CD3, GM1, as well as their
nanospatial relationship and their correlation with PKCθ
signaling cascade during T-cell activation. Interestingly, after
anti-CD3/anti-CD28 Ab co-stimulation, both TCR/CD3 and GM1 were
clustered to form nanodomains; moreover, all of TCR/CD3
nanodomains were colocalized with GM1 nanodomains, indicating
that the formati