Figure 2.  Effect of increasing priming complexity reverse
transcription using FPE tissue RNA template. Eight GSP pools
containing from 94 to 96 unique primers were prepared. Two pools
were selected at random to prime separate RT-PCR reactions using
RNA from FPE tissue. The 8 GSP pools were then combined to make
a single GSP pool that was used to prime one RT-PCR reaction
using the same FPE RNA template. The data therefore represents
concordance between primings for the subset of gene assays
represented with those two GSP pools. Both priming methods were
performed twice and the average CT values determined for this
analysis. The solid line represents the least squares line fit
and the dashed line represents the line of concordance.  Clark-
Langone et al. BMC Genomics 2007 8:279  
doi:10.1186/1471-2164-8-279 Download authors' original image