Figure 3.  Intracellular trafficking networks associated with
golgi, plasma membrane, and endosomes that have differentially
expressed genes in glaucoma astrocytes. (a) Schematic
representation of the intracellular trafficking network.
Upregulated mRNAs have a large red node and font, while
downregulated genes have a large blue node and font. Small black
nodes and font indicate genes that have 'present calls' without
differential expression. (b) Confirmation of four differentially
expressed genes from the trafficking network by qRT-PCR in human
ONH astrocytes: RAB4A, RAB5B, HAPLN and VCAN. Genes were
normalized to 18S RNA. Graphical representation of the relative
mRNA levels in normal and glaucomatous AA and normal and
glaucomatous CA astrocytes (n = 6, two-tailed t-test). Asterisk
indicates p < 0.05. (c) Representative double immunofluorescent
staining of versican (VCAN; red) and astrocyte marker GFAP
(green) in sections of human ONH from an AA donor (51 year old
female), AAG donor (70 year old male), CA don