Antibody Storage  General comments:  Antibodies, like most
proteins, do not like to be freeze-thawed. Avoid repetitive
freezing of your solution. The best way to store your antibody
is to keep a high protein concentration (>1 mg/ml), add some
protease inhibitors, and aliquot it for use. Then freeze the
aliquots and keep just one around for day to day use at four
degrees C.  Raw serum containing antibodies:  Aliquot into
reasonable aliquots (usually 10-50 ml) appropriate to your use
of the antibody and freeze aliquots; most serum can be stored
safely for months at four degrees with little loss of activity.
Due to the presence of protease inhibitors in serum little
proteolysis is observed and high protein concentrations keep the
antibody in a favorable environment with little denaturation.
Purified antibodies:  Once purified, if the antibody is at less
than 0.1 mg/ml add a small amount of detergent or protein (0.1%
TWEEN-20 or 1% bovine serum albumin) and some protease
inhibitors to the solution to protect the