Molecular characterization of Cryptosporidium species at the
wildlife/livestock interface of the Kruger National Park, South
Africa.  Epidemiology Section, Department of Production Animal
Studies, University of Pretoria, Private Bag X04, Onderstepoort
0110, South Africa. Electronic address: . Comparative
immunology, microbiology and infectious diseases (Impact Factor:
2.99). 09/2012; DOI:10.1016/j.cimid.2012.07.004 Source: PubMed
ABSTRACT Molecular characterization of Cryptosporidium spp. was
done on isolates from African elephant (Loxodonta africana),
African buffalo (Syncerus caffer), impala (Aepyceros melampus)
and native domestic calves collected during May and June 2008 at
the wildlife/livestock interface of the Kruger National Park
(KNP), South Africa. A polymerase chain reaction (PCR)
restriction fragment length polymorphism (RFLP) analysis of the
18S rRNA gene was used in feces from 51 calves (3-12 months of
age), 71 buffalo, 71 impala and 72 elephant, and sequencing of
the 18S rRNA gene was done on