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Select a Filter Below  Year of Publication more » 1.  Heparin
Modulates the Endopeptidase Activity of Leishmania mexicana
Cysteine Protease Cathepsin L-Like rCPB2.8  PLoS ONE 
2013;8(11):e80153. Cysteine protease B is considered crucial for
the survival and infectivity of the Leishmania in its human
host. Several microorganism pathogens bind to the heparin-like
glycosaminoglycans chains of proteoglycans at host-cell surface
to promote their attachment and internalization. Here, we have
investigated the influence of heparin upon Leishmania mexicana
cysteine protease rCPB2.8 activity. Methodology/Principal
Findings The data analysis revealed that the presence of heparin
affects all steps of the enzyme reaction: (i) it decreases
3.5-fold the k1 and 4.0-fold the k−1, (ii) it affects the acyl-
enzyme accumulation with pronounced decrease in k2 (2.7-fold),
and also decrease in k3 (3.5-fold). The large values of ΔG  = 
12 kJ/mol for the association and di