 PLoS One. 2009; 4(7): e6323. Published online Jul 28, 2009.
doi:  10.1371/journal.pone.0006323 PMCID: PMC2712089  Mapping
Accuracy of Short Reads from Massively Parallel Sequencing and
the Implications for Quantitative Expression Profiling  Rodolfo
Aramayo, Editor    Massively parallel sequencing offers an
enormous potential for expression profiling, in particular for
interspecific comparisons. Currently, different platforms for
massively parallel sequencing are available, which differ in
read length and sequencing costs. The 454-technology offers the
highest read length. The other sequencing technologies are more
cost effective, on the expense of shorter reads. Reliable
expression profiling by massively parallel sequencing depends
crucially on the accuracy to which the reads could be mapped to
the corresponding genes.  Methodology/Principal Findings  We
performed an in silico analysis to evaluate whether incorrect
mapping of the sequence reads results in a biased expression
pattern. A comparison of six avai