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processing data from triplicate runs - (Apr/24/2008 )   I am
doing QPCR using the Light Cycler and SYBR Green. I am using
several housekeeping genes and their geometric mean as a
normalization factor for my GOI. I run my samples in
triplicates.  The question is, should I use the 3 values of the
triplicates when they differ? how much they can differ and be
included? I mean, if two values are very similar and one
deviates, do I remove this third one? and what if the three
deviate from each other, how much they can deviate? And also, do
you calculate the mean or the median from these values?     If I
see a curve in replicates that differs to much from the other
two, I exclude it from calculation, because there definitely
went something wrong. It more a question of how you are used to,
I usualy take Ct deviation to 0.1 as very good, to 0.5 usable
and higher ones as problematic, but this could be different in
different assays.  Usualy higher Cts differ mo