Join for Free! 104647 members  Can any one clarify my question
with DNA   Moderator: BioTeam  Can any one clarify my question
with DNA  Postby shivakumar » Fri Mar 14, 2008 6:29 am  hi
everybody  while extracting DNA, extraction buffer containing
NaCl, EDTA, Tris n CTAB whts d role of those chemicals n n why
adjusting d buffer pH to 8  n I feel same doubt with chloroform
isoamyl alcohol(their role) Posts: 7 Joined: Thu Mar 13, 2008
11:51 am  Postby canalon » Fri Mar 14, 2008 3:25 pm  I can
clarify your question. I would use good syntax and complete
words. It would like that:  "In a protocol for the extraction of
DNA we use the following reagents, could you tell me what are
their different roles? NaCl, EDTA, Tris, CTAB,
Chloroform/Isoamyl alcohol. Similarly why is the pH adjusted to
8?"  Now as a beginning of an answer: NaCl is used to prevent
the co-precipitation of DNA and CTAB. EDTA is used to chelate
divalent cations, which are necessary (among other things) for
proper DNASe activity Tris is a Buffer CTAB