Sun Feb 11 18:40:23 EST 1996  Dr. Spagnol asked the following
question:  "the glycoprotein I'am study is esxpressed in a cell
line and is fucosylated in the chitobiose core. I would like to
ihnibit fucosylation of N-Linked chains in these cultured
cells."  In most cases, fucosylation of core GlcNAc takes place
on complex oligosacchari-des.  If you don't care about the
carbohydrate structure (i.e., high mannose vs. complex), there
are two ways to avoid such a fucosylation in over-expression.
1) express it in the same cell line and in the same culture
condition, exceptthat a glycosidase inhibitor (e.g.
deoxymannojirimycin for mannosidase I) is  included; or 2)
express it in a mutant cell line that only produce high mannose
structures (e.g., Lec1 CHO cells).  In my case of overexpression
for a lysosomalenzyme, alpha-L-iduronidase from a secreting CHO
cell line, I found a very effective reduction of fucosylation in
the presence of deoxymannojirimycin, which does not affect my
enzyme's biosynthesis, secretion, a