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Protocol    1. Determine cell concentration using hemacytometer
1. Take out cell line     2. Remove specified amount from flask
and transfer to 50 mL centrifuge tube     3. Take 10 microliter
sample and put into eppendorf tube     4. Add 10 microliters of
trypan blue to tube and mix     5. Take 10 microliters and add
to hemacytometer     6. Count number of cells in each 4 x 4
grid, and divide by 4. Multiply by 2 (dilution factor)     The
number obtained multiplied by 10^4 is the cell concentration
2. Put cells in each well of the plate at a concentration of
2.5*10^5 mL (50,000 per well)     1. Centrifuge specified amount
of cells in media (3 g 5 min 4 deg C)     2. Decant and Wash
with PBS     3. Centrifuge and resuspend in media without serum
4. Dilute conce