BISC209: Lab6  From OpenWetWare  Revision as of 12:47, 15 March
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search Wellesley College-BISC 209 Microbiology -Spring 2010
LAB 6: Start the Characterization of Cultured Bacteria by
16srDNA Sequencing and Analysis & Continue Traditional ID
Techniques  In addition to isolating genomic DNA from a soil
sample, amplifying the 16S rDNA by pcr, inserting the different
DNA fragments from the pcr product into a cloning vector,
transforming E. coli with your clones, and sending the
transformed E. coli off to have the 16s rDNA inserts sequenced
in an automatic sequencer so that you can identify a large and,
we hope, representative sample of the bacterial flora in the
soil community from your habitat, you also have been working,
simultaneously, through traditional microbiological culturing
techniques, to isolate and characterize a few of the culturable
bacteria through morphology, physical, and metabolism
differentiation. Look how much you have accomplished in