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Antibody Phage Display Assisted Identification of Junction
Plakoglobin as a Potential Biomarker for Atherosclerosis  PLoS
ONE  2012;7(10):e47985. To date, no plaque-derived blood
biomarker is available to allow diagnosis, prognosis or
monitoring of atherosclerotic vascular diseases. In this study,
specimens of thrombendarterectomy material from carotid and
iliac arteries were incubated in protein-free medium to obtain
plaque and control secretomes for subsequent subtractive phage
display. The selection of nine plaque secretome-specific
antibodies and the analysis of their immunopurified antigens by
mass spectrometry led to the identification of 22 proteins. One
of them, junction plakoglobin (JUP-81) and its smaller isoforms
(referred to as JUP-63, JUP-55 and JUP-30 by molecular weight)
were confirmed by immunohistochemistry and immunoblotting with
independent antibodies to be present in a