Cell Lysing Detergent  Fergus R. McKenzie mckenzie at unice.fr
Tue Nov 5 12:38:27 EST 1996  Subject: Cell Lysing Detergent
From: Rick Bright, rbright at mnw.net Date: Thu, 31 Oct 1996
18:49:00 -0600 In article <327948FC.4B98 at mnw.net> Rick
Bright, rbright at mnw.net writes: >I have a DNA extraction
method that calls for Nonidet P-40 as the lysing >agent.
However, I cannot find a source in Fisher, Sigma, or Pharmacia.
>Does anyone have a source or catalog number for this detergent.
>Also, is there a particular reason why one would use Nonidet
P-40 over >SDS or Triton X-100 for lysing blood cells?  Are
there particular >applications where one agent is better than
others, or are do they serve >the purpose equally well for all
applications? >I appreciate your assistance. >Rick Bright
>rbright at mnw.net  Dear Rick,
In response to previous comments.. Nonidet P-40 and NP-40 are
NOT the same thing:          NP-40 is NONYLphenoxy polyethoxy
ethanol          Nonidet P-40 is OCTYLphenoxy ....          You
can buy both at the Sig