SGD Paper Help  Tang Y, et al.  (2008) Fungal Rtt109 histone
acetyltransferase is an unexpected structural homolog of
metazoan p300/CBP. Nat Struct Mol Biol 15(7):738-45  Abstract:
Rtt109, also known as KAT11, is a recently characterized fungal-
specific histone acetyltransferase (HAT) that modifies histone
H3 lysine 56 (H3K56) to promote genome stability. Rtt109 does
not show sequence conservation with other known HATs and depends
on association with either of two histone chaperones, Asf1 or
Vps75, for HAT activity. Here we report the X-ray crystal
structure of an Rtt109-acetyl coenzyme A complex and carry out
structure-based mutagenesis, combined with in vitro biochemical
studies of the Rtt109-Vps75 complex and studies of Rtt109
function in vivo. The Rtt109 structure reveals noteworthy
homology to the metazoan p300/CBP HAT domain but exhibits
functional divergence, including atypical catalytic properties
and mode of cofactor regulation. The structure reveals a buried
autoacetylated lysine residue that we sho