From 2010.igem.org  How to use the granule ?  Composition of the
granules  Figure 1 : PHB granule. (Grage et al.,
Biomacromolecules, 2009)   The formation steps of those granules
are not well determined, but their final structure and
composition have been described. The three PHB biosynthesis
genes are organized in one operon in the Ralstonia eutropha H16
strain: the phaCAB operon. In addition to the three essential
enzymes for the PHB granules biosynthesis, other proteins
contribute to the regulation and the formation of the granules.
They can be separated in three classes: the depolymerases
(PhaZ), the regulatory proteins (PhaR) and the phasins (PhaP).
They are located on the fatty surface of the granule (see on
Figure 1).  PhaPs, also called phasins, are proteins regulating
the size and the morphology of granules and preventing the
fusion of PHB granules by formation of a protein layer between
the hydrophobic polymer and the hydrophilic cytoplasm. Their
expression is regulated by the PHB accumulation, than