SGD Paper Help  Candau R, et al.  (1997) Two tandem and
independent sub-activation domains in the amino terminus of p53
require the adaptor complex for activity. Oncogene 15(7):807-16
Abstract: The ability of p53 to function as a tumor suppressor
is linked to its function as a transcriptional activator, since
p53 mutants that do not transactivate are unable to suppress
tumor cell growth. Previous studies identified an activation
domain in the amino terminal 40 residues of the protein, a
region that binds to several general transcription factors and
to some oncogene products. For example, mdm-2, a cellular
oncoprotein, binds to this region and represses p53
transactivation. Here we describea new activation domain within
the amino terminus of p53 that maps between amino acids 40-83,
and whose residues trp-53 and phe-54 are critical for function
both in yeast and in mammalian cells. In vivo studies in yeast
show that the new activation subdomain, unlike the previously
described, is mdm-2 independent. Both p53 a