Protocol Online logo  How long did you treat DNA with bisulfite?
- (Aug/26/2005 )  I do my experiment by follow this method  I
would like to ask everybody. 1. How long did you treat dna with
bisulfit? 2. what is the minimum and maximum time in bisulfite
treatment? 3. glycogen is necessary or not?  Thanks for you
help.    i treat my DNA overnight for 12-16 hours. there are
bisulfite modification kits that have minimum conversion times
of four hours which means you can effectively complete the
conversion in one day (methyleasy).  Glycogen is not a necessary
but helps increase the yield of DNA when precipitating, I
usually add it in to make sure as it doesn't hurt downstream
reactions anyeays.