troubles with PCR  Ari Kahn ak01706 at Fri Jan 26 17:32:49 EST
1996  I am studying genetic diversity in wild rice by looking at
intronic  regions between tandemly repeated nrDNA.  Using PCR,
one species  (Zizania aquaticus) amplifies readily, another
species (Zizania  pelustris) amplifies sometimes, and the last
species (texana) I can  not get to amplify.         I figure
since all my reaction conditions are the same between each
species that my problem with amplification lies in my DNA
samples.   So, assuming this, I ran out my stock and dilution
samples on a gel  to visualize them.  I noticed two things:  One
is that the molecular  weight of the samples differed; i.e.
pelustris had the highest  molecular weight DNA (a tight band on
top), followed by aquaticus  (it was a continuous streak), and
then texana had the lowest (with  most of the streak towards the
bottom).  Two is that aquaticus had  very little tRNA visible,
while in pelustris and aquaticus the tRNA  was obvious.
I think the reason I¹m not able to amp