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Identification of Candida Species Associated with Vulvovaginal
Candidiasis by Multiplex PCR  Background. Vulvovaginal
candidiasis is a common infection. The aim of this study was to
identify the species of vaginal Candida isolates by using
multiplex PCR technique. Methods. 191 isolates from patients
admitted to Mahdieh hospital were identified. The vaginal swab
specimens were cultured on Sabouraud Dextrose Agar. The ITS1
region between the 18S and 5.8S rRNA genes and a specific DNA
fragment within the ITS2 region were amplified. The multiplex
PCR products were separated by electrophoresis in 2% agarose
gel, visualized by staining with ethidium bromide, and
photographed. Descriptive statistics, Chi-square test, and
Spearman correlation were used to summarize the findings.
Results. C. albicans and C. glabrata were the most common
species isolated from the specimens. A mix of C. glabrata and