  Durham e-Theses You are in:  Elucidation of the structure and
mechanism of the MtrCDE multi drug transporter in Neisseria
gonorrhoeae  Zhang, Li (2008) Elucidation of the structure and
mechanism of the MtrCDE multi drug transporter in Neisseria
gonorrhoeae. Doctoral thesis, Durham University.    The Mtr
(Multiple transferable resistance) transport system in Neisseria
gonorrhoeae was found to confer the resistance of gonococci to
penicillin and structural diverse hydrophobic agents (HAs), such
as drugs, dyes, detergents and host-derived compounds (fatty
acids and bile salts), as well as some cationic antimicrobial
peptides. The mtr operon encodes an energy-dependant efflux pump
system and this MtrC-MtrD-MtrE system is negatively regulated by
the transcriptional repressor MtrR. The first part of this
thesis presents insights into a structural study of the two
membrane components, the inner membrane protein MtrD and the
outer membrane protein MtrE. MtrD and MtrE have been
overexpressed, purified and crystalliz