Analysis of DNA replication parameters in isolated organs of
drosophila melanogaster by molecular combing  Cell and Tissue
Biology 04/2012; 5(1):1-8. DOI:10.1134/S1990519X11010081
ABSTRACT Methods of DNA physical mapping and the direct
visualization of the replication and transcription in specific
genome regions play a crucial role in the study of structural
and functional organization of eukaryotic genomes. Since DNA
strands in cells are organized into high-fold structure and
structurally present as highly compacted chromosomes, the
majority of methods at the chromosome level are of low-
resolution. An approach to increasing the resolution and
accuracy is molecular combing. The method is based on the
process of stretching and alignment of DNA molecules that are
covalently attached with one of the ends to the coverglass
surface. In this article, we describe the major methodological
steps of molecular combing and their adaptation to study the
parameters of DNA replication in polyploid and diploid tissues
of dr