Saponin Lysis of RBCs  From OpenWetWare  Jump to: navigation,
search    Jingyang Chen, Seattle Biomedical Research Institute,
307 Westlake Ave N, Suite 500, Seattle, WA, 98109, USA.    Lyse
red blood cells while leaving the Plasmodium falciparum parasite
intact with it's parasite membrane and parasitophorous vacoule
membrane. Typically used right before freezing down parasites
for genomic DNA extraction, or for getting rid of hemoglobin
right before running a Western Blot on parasite extracts.     •
0.15% Saponin in PBS   • 1X PBS   • 12 mL of Plasmodium
falciparum blood culture at 4% hematocrit     • Refrigerated
centrifuge capable of holding 15 mL conical tubes   •
Microcentrifuge     1. Centrifuge parasite culture at 1400 rpm
(~484xg) for 3 minutes.   2. Aspirate media.   3. Resuspend
pellet in 1 ml of 0.15% Saponin (in aliquots in freezer) and
transfer to a 1.5 ml eppendorf tube.   4. Incubate for 5 minutes
on ice and vortex each minute.   5. Spin at 6000 rpm for 3
minutes at 4 degrees C.   6. Wash with 1