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Proteomics  Antibodies for different epitope giving bands of
different sizes in the same sam - (Nov/11/2007 )   I'm been
trying to detect a Protein, using 2 sc antibodies, one recognize
N-term, the other the C-term. I've bands of 2 different sizes
with these Abs using the same samples (50kD and ~62kD)! In
addition, transfected cells for the protein shows yet another
size (~55kD) (but in this case, both Abs give the same size.).
Can anyone offer any explanation?    This could be an
explanation.  Probably, the antibodies are detecting a different
variant of the same protein. And when you transfect the cells,
you are using only a single variant transgene, so the antibodies
detect only 1 of them.