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Access  Abstract Recently, we described a novel fibroblast-
restricted monoclonal antibody (mAb AS02) that recognizes a
membrane-bound antigen. Characterization and isolation of the
corresponding antigen showed that mAb AS02 recognized a protein
on human fibroblasts that is highly homologous or identical to
human Thy-1 antigen (CD90). Partial amino acid sequencing of the
corresponding mAb AS02 antigen and comparison with known
proteins revealed a 100% homology of the sequenced peptides to
the human Thy-1 antigen. Cross-immunodepletion studies with mAb
AS02 and an anti-Thy-1 antibody confirmed these results.
Utilizing two-dimensional (2D) gel electrophoresis of fibroblast
cell extracts and purified antigen, mAb AS02 and the anti-
Thy-1-antibody recognized identical protein spots. Furthermore,
we demonstrated many identical bi