SGD Paper Help  Hedges J, et al.  (2005) Release of the export
adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p
and the cytoplasmic GTPase Lsg1p. EMBO J 24(3):567-79  Abstract:
In eukaryotes, nuclear export of the large (60S) ribosomal
subunit requires the adapter protein Nmd3p to provide the
nuclear export signal. Here, we show that in yeast release of
Nmd3p from 60S subunits in the cytoplasm requires the ribosomal
protein Rpl10p and the G-protein, Lsg1p. Mutations in LSG1 or
RPL10 blocked Nmd3-GFP shuttling into the nucleus and export of
pre-60S subunits from the nucleus. Overexpression of NMD3
alleviated the export defect, indicating that the block in 60S
export in lsg1 and rpl10 mutants results indirectly from failing
to recycle Nmd3p. The defect in Nmd3p recycling and the block in
60S export in both lsg1 and rpl10 mutants was also suppressed by
mutant Nmd3 proteins that showed reduced binding to 60S subunits
in vitro. We propose that the correct loading of Rpl10p into 60S
subunits is requir