Figure 3.  Candidate motifs identified upstream of the
micronemal protein-encoding genes, upstream location, site-
directed mutagenesis and results of reporter assays. Motifs MICA
and MICB display an additive effect in the regulation of the
gene encoding microneme 8. (a) Sequence logos represent the
consensus sequence for each candidate motif. The y-axis
represents information content at each position. (b) Occurrences
and positions of the motifs in the promoter region relative to
the translational start site of each gene. The gene names are
abbreviated as shown in Table 1. The underlined gene name
indicates the representative promoter used in reporter assays.
Motif MICA, found in both E. tenella and T. gondii, is denoted
by a circle and motif MICB, exclusive to T. gondii, is denoted
by a square. (c) The WT motifs and their mutagenized (MUT)
versions in the representative promoter are represented. (d) The
graphs depict luciferase activity as ratios of firefly:renilla
activity in relative luciferase units (RLU)