RE: Cryostat sectioning  From:Cathy Gorrie <>  <!doctype html
public "-//W3C//DTD W3 HTML//EN"> <html><head><style
type="text/css"><!-- blockquote, dl, ul, ol, li { margin-top: 0
; margin-bottom: 0 } --></style><title>RE: Cryostat
sectioning</title></head><body> <div>Firstly, I would check
that<u> everything</u> is done up tight. All the problems you're
described can happen when something is loose.</div>
<div>Cathy</div> <div><br></div> <div>At 11:26 AM -0400 4/7/01,
wrote:</div> <blockquote type="cite" cite><font face="Arial"
size="-1">I have been having some touble while sectioning fresh
frozen tissue this<br> week. I have tried several different
things to rememdy, nothing working. Has<br> anyone else
experienced these problems? And how where they resolved.<br> 1.
The tissue is splitting in half, all the way across the face of
the block.<br> Not the up and down which would indicate to move
the blade.<br> 2. Bad problem with thick and thin sections. I've
sprayed the tissue to bring<br> down the temp and make