Definition: Saturation Mutagenesis  Rock Pulak rapulak at Tue
Mar 7 12:42:03 EST 1995  In article <anderson-060395172733 at>
Eric C. Anderson, anderson at writes: >so far, all i've been
able to find in the way of a definition or >description came
from Lewin's Geves IV where they consider it the >of every
nucleotide in a particular region.  my personal vote would be
>mutating every amino acid to something different, preferably
not a >conservative change.  If your gene was a tRNA gene, then
how would you define saturation mutagenesis?  Do you think the
term should have gene specific meanings? (IMHO) I don't think it
should.  Also, it may just be a poor term in general.  Should
saturation mutagenesis be every nucleotide for every other
nucleotide, one  at a time, then two at a time, then three at a
time, and so forth? Real "saturation" of all possible mutations
would be just that. Some of forms of SELEX achieve this.  Most
site directed mutagenesis experiments fall far short of these
goals.  Rock Pulak  More inf