Figure 1 shows the typical line shape for electronic transitions of individual chromophores in a solid matrix. The zero-phonon line is located at a frequency ω’ determined by the intrinsic difference in energy levels between ground and excited state as well as by the local environment. The phonon sideband is shifted to a higher frequency in absorption and to a lower frequency in fluorescence. The frequency gap Δ between the zero-phonon line and the peak of the phonon side band is determined by Franck–Condon principles.