Lady Octopus appears in the Spider-Man episode "Between an Ock and a Hard Place", voiced by Kari Wahlgren. This version has a Ph.D in electronics which she used to fake her criminal record and have herself put in a cell next to Doctor Octopus in superhuman prison called the Cellar where he manipulates her into obtaining crystals for a modified neuro cortex device for mind enhancement. After Spider-Man defeats her, she learns she had been manipulated and seeks revenge. To achieve this, she and Spider-Man fight Doc Ock and put her former idol into a coma. Following the fight, she relinquishes her tentacle harness and leaves.