Endesfelder was born on 4 July 1983. From 2002 to 2008, she studied Physics, Astronomy, and Microbiology at the University of Bonn in Bonn, Germany and Stony Brook University, NY, USA. In 2012, she earned her Ph.D. in Physics at the Department of Applied Laser Physics and Laser Spectroscopy at Bielefeld University, Germany.