In 2018, Tyson made a second guest appearance on The Big Bang Theory as himself, together with fellow television personality Bill Nye, in the first episode of the show's final season ("The Conjugal Configuration"). He also had guest appearances in Gravity Falls, Brooklyn Nine-Nine, Zoolander 2, Ice Age: Collision Course, Family Guy, BoJack Horseman, The Simpsons, Salvation and Scooby-Doo and Guess Who?.