Cifarelli was born in Rome in 1952, daughter of Michele Cifarelli, an Italian politician and magistrate. She studied physics at the University of Bologna and graduated in 1975. She worked as a researcher in at the Istituto Nazionale di Fisica Nucleare and CERN. She edited the collection of scientific studies for the publication QCD at 200 TeV. In 1988 she was made an associate professor at L'Università degli Studi di Napoli Federico II.