While gluons are massless, they still possess energy – chromodynamic binding energy. In this way, they are similar to photons, which are also massless particles carrying energy – photon energy. The amount of energy per single gluon, or "gluon energy", directly measured, though a distribution can by inferred from deep inelastic scattering (DIS) experiments (see ref [4] for an old but still valid introduction.) Unlike photon energy, which is quantifiable, described by the Planck–Einstein relation and depends on a single variable (the photon's frequency), no simple formula exists for the quantity of energy carried by each gluon. While the effects of a single photon can be observed, single gluons have not been observed outside of a hadron. A hadron is in totality composed of gluons, valence quarks, sea quarks and other virtual particles.