The working principle of molography is illustrated in Figure 1. Molography is based on diffraction of laser light at a special 2D nanopattern of molecular binding sites on the surface of a sensor chip, termed mologram. A mologram is a coherent assembly of binding sites on a chip that form the blueprint of molecular hologram. The hologram has the shape of a focusing diffractive lens and is illuminated by an evanescent wave. Evanescent waves occur when light is totally internally reflected at an interface of two dielectrics. Biomolecules that bind to the mologram diffract laser light into a diffraction-limited focal spot in three dimensional space, the focal point of the mologram. The intensity of the focused light correlates quadratically with the amount of bound molecules on the mologram and therefore also the number of biomolecular interactions that take place.