Nollet tried to seal wine containers with a pig's bladder and stored them under water. After a while the bladder bulged outwards. He noticed the high pressure that discharged after he pierced the bladder. Curious, he did the experiment the other way round: he filled the container with water and stored it in wine. The result was a bulging inwards of the bladder. His notes about this experiment are the first scientific mention of permeation (later it would be called semipermeability).