Freedhoff enrolled in the Mathematics, Physics, and Chemistry stream at the University of Toronto, one of around 10-15 women among 120 first year students. Originally intending to study mathematics, she found that she preferred physics. Freedhoff was the only woman in her year to major in physics, graduating with the highest marks and being awarded the Governor General's Gold Medal. She did not feel professionally disadvantaged by being the only woman, and felt it could be an advantage to stand out.