Lakadamyali's area of research is focused on examining biology at the level of its macromolecular machines. She aims to obtain a quantitative and biophysical comprehension of how these machines propel critical cell biological processes. Hence, she is also involved in designing sophisticated microscopy techniques that strive to surmount the current limitations of existing methods, thereby enabling them to observe the macromolecular machinery of the cell in motion with superior spatiotemporal resolution.