Lady Octopus appears as a member of a female incarnation of the Sinister Syndicate. She asks Francine Frye if she is familiar with the concept of macrobiotics. Carolyn then mentioned to Janice Lincoln about the e-mail about the refrigerator where there is dairy products inside where she was planning an organic vegan cheeseburger night on Friday. The Sinister Syndicate begins their mission where they attack the F.E.A.S.T. building that Boomerang is volunteering at. Beetle leads the Sinister Syndicate in attacking Boomerang. It was stated by Boomerang that he was the one who came up with the Sinister Syndicate name. Lady Octopus stated that they dropped the "Sinister" part of the Sinister Syndicate's name. After getting Aunt May to safety, Peter Parker changes into Spider-Man and helps Boomerang fight the Syndicate. The Syndicate starts doing their formation until Spider-Man accidentally sets off Boomerang's gaserang which knocks out Spider-Man enough for the Syndicate to make off with Boomerang. When Beetle returns to the headquarters, Lady Octopus is present when Mayor Wilson Fisk brings the full force of New York City to their headquarters demanding that they surrender Boomerang to him. The Syndicate then assists Spider-Man against Mayor Fisk's forces. After Spider-Man evacuates Boomerang, the Syndicate fights Mayor Fisk's forces while not killing them. The Syndicate is defeated and arrested by the police. Their transport is then attacked by an unknown assailant who frees them.