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Antimicrobial resistance: preventive approaches to the rescue? Professional expertise and business model of French “industrial” veterinarians

This article focuses on the development of veterinary medicine in the industrial pig and poultry production sector. In the current context of controversies over the public problem of antimicrobial resistance (AMR), the veterinary profession is tending to promote a model of preventive medicine that is supposed to reduce the use of antibiotics in livestock farming. However, veterinarians specializing in pig and poultry production (“industrial vets”) have in fact been adopting such approaches to animal health for several decades. Based on 28 interviews with pig and poultry veterinarians practicing or having practiced in western France between the 1970s and the 2010s, the article aims to understand how such a form of professional expertise has developed, and the business model that underpins it. Contrary to public discourses which promote preventive approaches as a way to diversify professional expertise and to disconnect veterinary incomes from drug sales, it is indeed this economic model that has allowed the development of such approaches within industrial livestock farming. Modern strategies for reducing antibiotic use should therefore seek less to renew the professional expertise of veterinarians than to find new ways to valorize it economically.

The French veterinary profession is currently undergoing major changes; or at least it tends to see itself as being at the heart of a period of major challenges that are pushing it to reinvent itself. This is not the first time that it has had to face such a reflexivity test, even in recent history (in the British case, some historians even see a cycle-Woods 2011a), but recent literature produced by professional veterinary organizations shows the importance of what is currently perceived as a need for self-analysis and change (ONDPV 2018;VetFuturs France 2018). There are several issues that might explain why this period is favourable to such a prospective assessment of veterinary futures. One of the most important concerns the controversies and public policies that have developed over recent years with regard to the issue of antimicrobial resistance (AMR), which has directly challenged the economic and professional model of farm animal veterinary practices that were setting drugs (mostly antibiotics) up as a cornerstone of veterinary activity, as a source of both income and professional expertise.
Cross-fertilization of research on the veterinary profession and drug regulation is not common. Although veterinarians have aroused the interest of certain historians and sociologists of professions, this has essentially been in relation to the analysis of this social group's process of professionalization (Berdah 2012;Mitsuba 2017), its role in animal health or food safety policies (Woods 2011b;Enticott et al. 2011;Fortané 2016, 2018), the dynamics that contribute to its specialization (Gardiner 2014) or feminization (Surdez 2009), or finally to knowledge and professional practices in farm (Shortall et al. 2016;Ruston et al. 2016) and small animal medicine (Sanders 1994;Morris 2012). As for the regulation of veterinary drugs, there are also several studies by historians on the vaccination of animals against major zoonoses or epizootic diseases (Woods 2004;Berdah 2018), sometimes on the veterinary pharmaceutical industry (Corley and Godley 2011), and more recently on the AMR issue (Kirchhelle 2018). Yet unlike the uses of human medicines that medical anthropology has been able to theorize and document for many years (Whyte et al. 2002), the uses of veterinary medicines, i.e. the conditions under which they are prescribed, dispensed and used, are rarely studied, except in interdisciplinary literature from the field of veterinary sciences (Speksnijder et al. 2015;Coyne et al. 2016).
This article tries to open a way to cross-fertilize these reflections. Using recent debates on the AMR problem, it proposes to examine the relationship between the development of professional veterinary expertise and of the drug market, based on the case of a specific segment of the profession, namely veterinarians specializing in industrial poultry and pig production in western France. It thus puts the AMR issue under a broader lens as it analyses ongoing changes within the veterinary profession not as potential consequence of recent measures aiming to reduce antibiotic use, but rather as a reason for the way the problem is now framed. Indeed, it is common to hear professional organizations or public authorities state that in order to reduce their economic dependence on antibiotic sales, vets must rethink their activity by favouring preventive approaches to animal health which would involve a diversified range of services and would contribute to placing vets in an advisory role with a holistic vision of livestock farming or even of the food supply chain (VetFuturs France 2018). However, such a form of professional expertise, combined with a particular business model for the practices promoting it, is not fundamentally new. If it is at the heart of contemporary debates, it is because it is based and supported by far earlier dynamics that initially had nothing to do with AMR, but which used the opportunity of current controversies surrounding antibiotic use, sale and prescription to reinforce and legitimize a certain vision of veterinary medicine, based on preventive approaches to animal health.
Poultry and pig medicine in industrial production is an especially interesting area for an analysis of these dynamics. Firstly, because approaches to animal health that have developed in this field are very singular and characteristic of the intensive farming methods used in these sectors, particularly in the Brittany and Pays de Loire regions where a large proportion of the production is located. Indeed, a certain vision of preventive medicine was developed by pig and poultry vets in the 1970s and 1980s, even if the issue of this form of veterinary expertise has not always been raised in these terms. This article therefore aims to understand the professional knowledge, practices and economic model upon which this kind of expertise is based, and why the current AMR context is an opportunity to expand it (or at least attempt to). Secondly, pig and poultry vets make up an extremely small and autonomous segment of the profession (which makes it possible to draw up a fairly representative picture) although its homogeneity should not be overestimated. This article thus seeks to provide a thorough analysis of this very particular part of the veterinary profession, the specificities of which have almost never been addressed by the literature. Despite their small numbers, "industrial" vets are nevertheless an essential component of the profession, because they manage the health of an economic sector which supplies a considerable share of national animal production.
The article opens with a brief presentation of the political context and controversies surrounding the AMR problem, and how the French veterinary profession has faced up to this by defending the preventive medicine model. It then describes this form of expertise in the professional segment studied here, showing why pig and poultry vets chose this specialization. The article then looks at the origins of these preventive approaches to animal health, both in terms of knowledge and practices, and the economic model associated with it. Finally, it reviews the strategies currently developed by industrial vets to adapt to the constraints of increased control of antibiotic use in livestock.
The AMR problem and the preventive "solution" The problem of antibiotic use in livestock farming is not new. As soon as these molecules were introduced in agriculture in the late 1940s, there was controversy concerning the development of resistant bacteria in animals and food, and the risks of human contamination (Bud 2007). Yet for several decades, this issue has been eclipsed by the belief in a permanent renewal of the therapeutic arsenal, consisting in thinking that the continuous discovery of new antibiotics would compensate for the development of increasingly resistant bacteria (Podolsky 2018). After the Swann report in 1969, a series of measures to control the use of antibiotics as growth promoters 1 was nevertheless adopted in Europe, progressively separating the molecules used in agriculture and human medicine (Kirchhelle 2018). But 20 years later, during the avoparcin crisis 2,3,4,5 these measures were considered ineffective (in the sense that they did not prevent the transmission of resistant bacteria between humans and animals) and the use of antibiotics as growth promoters was finally banned in the European Union in 2003 6 (Kahn 2016).
The problem of antibiotic use in livestock farming as we know it today reemerged in the late 2000s, this time focusing on veterinary uses, i.e. on curative or preventive uses with veterinary prescription (Fortané 2019). Veterinarians were directly accused of being responsible for the overuse and misuse of antibiotics (and therefore for the spread of resistant bacteria) on the basis of a fairly simple argument: their supposed professional "conflict of interest". Indeed, in France, since the 1975 Act on veterinary pharmaceuticals, vets have had a dual monopoly on the prescription and supply of medicines (Hubscher 1999). Even if, in theory, delivery is shared between three beneficiaries (veterinarians, pharmacists and approved co-operatives), vets capture the vast majority of the curative drug market (of which antibiotics constitute the main category) (Guillemot and Vandaële 2009). The argument that veterinarians over-prescribe antibiotics in order to increase their incomes then became the main framing of the AMR problem.
This construction of the problem was in reality carried by a coalition of human health actors (doctors, pharmacists, health administration) whose political agenda was twofold. On the one hand, they defended a measure that crystallized the debates around the years 2009-2013: the "decoupling" of prescription and delivery, which consists in applying the professional and economic model that prevails on the human drug market, i.e. reserving prescriptions to physicians and sales to pharmacists. Decoupling basically means forbidding veterinarians from selling pharmaceuticals, as is the case in countries such as Sweden or Spain (Fortané 2016). On the other hand, this coalition supported the concept of "critically important antibiotics", the principle of which is to reserve certain molecules, in particular the latest generations of antibiotics, for human medicine. From a political and institutional point of view, this period was extremely interesting because it put the spotlight on definitional and jurisdictional conflicts between different social groups for the control of the legitimate use of antibiotics. It finally ended in a relative victory for veterinarians who succeeded, at the end of an unprecedented mobilization, in reversing the stigma that human health stakeholders assigned to them. Indeed, vets have been able to impose the image of a profession that is not guilty of overusing antibiotics but which is instead accountable for their proper use. The notions of prudent, judicious, rational or responsible use, now widely used in AMR debates, are thus a social construct produced by conflicts between social groups for the definition of the legitimate use of antibiotics (Fortané 2019).
This veterinarian victory led to the withdrawal of the two emblematic measures (decoupling of prescription and delivery; ban on critically important antimicrobials) supported by the coalition of human health actors. In return, a stricter framework for the use of antibiotics in animal husbandry was implemented between 2014 and 2016: margins on the sale of antibiotics are now limited and the retail price of antibiotics must be the same for every client, 7 and antimicrobial susceptibility tests are mandatory for the prescription of critically important antibiotics. 8 But once again, the most important part of this victory certainly concerns the changes regarding the image of the profession. Indeed, veterinarians not only reversed the stigma and positioned themselves as guardians of antibiotics, they were also able to re-appropriate the problem by highlighting the way they could solve it. Without denying that the economic model of the profession was too financially dependent on antibiotic sales, and that antibiotic use may have been too prevalent in animal care in the past, vets started to defend the development of preventive approaches which would, according to them, be the only way to ensure the transition towards an economic and professional model guaranteeing responsible use of antibiotics. This view was reinforced and supported by farmers and public authorities who also called for the development of such approaches which are usually promoted by national AMR policies ( Fig. 1) in France and in other countries (Badau 2016;Piquerez 2019). 9 At the heart of this new prospective narrative for the profession and its role in managing the AMR problem, we can observe the construction of a (supposedly) new conception of animal health, which is not based on a strictly clinical approach to diseases but on a holistic vision of animals and livestock farming (biosecurity, hygiene, nutrition, good husbandry practices, etc.). In the posters above, veterinarians are portrayed as the gatekeepers of such an approach, through their transversal role as "health advisors". This professional model, based on the knowledge and practices of preventive approaches to animal health, goes hand 7 Law for food, agriculture and forestry n°2014-1170 of October 13th, 2014. 8 Decree no. 2016-317 of March 16th, 2016. 9 When public authorities and the veterinary profession began to conceive this campaign, a third poster was designed, saying: "my vet is much more than a mere drug supplier, he is also a teacher! He prescribes the medicines I need … but above all he talks to my farmer to make sure that I am perfectly fed, sheltered and vaccinated". However this poster was not retained for the campaign because of its relatively sensitive headline. in hand with an economic model where the incomes of veterinary businesses would be more diversified since these new "health advisors" would be able to monetize a wider range of goods and services (hygiene and nutrition products, bacteriological analyses, livestock audits, etc.) than just pharmaceuticals.
Could we look beyond the symbols and images of professional and political discourses and see whether these preventive approaches that might change the way veterinary medicine is practiced, and how antibiotics are used, rely on actual knowledge and practices and, if so, where and since when? The fact is that this model of preventive veterinary medicine seems in reality to be quite typical of a very particular segment of the veterinary profession, the one this article proposes to describe, namely industrial vets. The remainder of the article thus seeks to address the following two questions: is this form of professional expertise really perceptible in the field, and is its development truly linked to the global context of the AMR problem and to recently implemented policy measures, or does it have other origins and raisons d'être that might actually 0 When public authorities and the veterinary profession began to conceive this campaign, a third poster was designed, saying: "my vet is much more than a mere drug supplier, he is also a teacher! He prescribes the medicines I need … but above all he talks to my farmer to make sure that I am perfectly fed, sheltered and vaccinated". However this poster was not retained for the campaign because of its relatively sensitive headline.

PromoƟng prevenƟve approaches to reducing veterinary use of anƟmicrobials
These two posters were used in the French 2016 AMR policy ("Plan EcoAnƟbio"). They are a perfect illustraƟon of how the veterinarian's role was reframed towards prevenƟve approaches to animal health (in parƟcular vaccinaƟon), as a means to reduce the use of anƟbioƟcs. The first poster (on the leŌ) says: "my vet is far more than a mere emergency doctor, he is an expert contribuƟng to good husbandry pracƟces". The second poster (on the right) says: "my vet is far more than a mere hands-on man, he is an advisor, always there to prevent and vaccinate". Both conclude with: "ask your vet for advice" 9 .

Being a vet in industrial and intensive livestock farming
When poultry or pig vets are asked why they chose this specialty, they often point out a huge contrast between what they do and the way they perceive cattle (i.e. "rural") or companion animal vets. They feel that being an "industrial vet" essentially relates to four characteristics.
Firstly, veterinarians specialized in poultry or pig medicine attach importance to working at the heart of the agri-food system, with livestock farming professionals. Many of them have agricultural family origins and believe that they chose this profession in order to maintain a strong link with the rural world. They perceive farmers as animal experts, unlike pet owners, and see their activity as teamwork alongside skilled professionals, with whom it is easier to interact and who can also provide them with knowledge about animals. Their clients are therefore also their partners in animal health management: they can trust them, rely on them and delegate tasks to them.
Secondly, pig and poultry vets consider their work to be a permanent renewal, as opposed to the repetitive and sometimes boring work of cattle vets who constantly reproduce the same gestures and who are rarely motivated or intellectually stimulated by new situations and new challenges. Most of them, whether they are young vets or already have 15 or 20 years of experience, tend to compare their professional activity with the image of the "emergency vet" (or "fire brigade" vet), available day and night to care for sick animals. From their point of view, this traditional activity is typical of cattle vets, corresponds to the past and relates to a type of work which is limited to clinical diagnosis, drug prescription and/or surgery (e.g. midnight calving). In their opinion, the only objective of such a way of working is to ensure that clients are satisfied with an occasional intervention and that they will once again call upon veterinary services the next time health problems occur. The point here is not to claim that this is what cattle vets are actually doing, 10 but simply to note that this narrative is widely used to define, by contrast, the professional identity of industrial vets.
I am indirectly from a rural family, that is to say that my grand-parents, my uncles, they all worked as farmers, in mixed farming and mixed animal farming, from both sides of my family. It is just my parents who had access during the postwar period to national education programmes and became teachers or researchers. So I am from the third generation but I spent a lot of time at the farm.
10 Although some of the characteristics that pig and poultry vets tend to associate with cattle vets have actually been observed in other studies, in particular the fact that cattle vets do not have a high opinion of the technical expertise of the farmers they deal with (Shortall et al. 2016). These opinions of their clients nevertheless depend to a certain extent on the type of farm and farmers they relate to: cattle vets have better professional relationships with "commercial farmers" (managers of large, modern and business-oriented farms) as the latter tend "to understand the need to use the vet as a disease prevention consultant rather than to treat individual sick animals: i.e. part of vets' desired move from a 'test and treat' to a 'predict and prevent' model of veterinary intervention" (Shortall et al. 2018, p. 597). In addition, this shows that a tension between "fire brigade" work and advisory veterinary work is also present within a certain section of the cattle vet profession.
I became a vet to take care of farm animals because I like being outside and having an intellectual occupation. So that is the reason why.

Poultry vet, 2015
So at vet school, you didn't think about working with dogs? Certainly not (laughs)! That was my nightmare! When you work in the pig sector, what is good is that you work 100% on farms. Because when you work with cattle, most of the time you have to do some small animal work as well. And what I want is to work with professionals who are producing the animals we eat. Pig farmers (well, not all of them but most of them) have very good technical skills. Pig farming is a very dynamic sector, you never get bored! We often have to upgrade the farms to new standards so it's always evolving. Research also moves very fast, the pharmaceutical industry innovates a lot so it's interesting. (…) And to be honest, what interests me the most is this kind of follow-up, not being a "fire brigade" vet. But I can't talk very knowledgeably about the "classic" rural vet as I never was one. But what I do think about this kind of practice is that there is a lot of emergency and "fire brigade" work and I don't think that is very challenging. In pig production, we have been evolving for quite a long time because if we only do "fire brigade" work… well, farmers expect a lot more than that! And for us, that is also what's interesting. Pig farming is a batch production, so we have this tendency to always try to do something for the next batch, try to prevent future batches from getting the disease we have now. So having to try this and that is always very dynamic and challenging. So we know that there is always a new batch to come, and that is not the same in cattle farming.

Pig vet, 2014
Thirdly, pig and poultry vets describe their work as being part of an epidemiological rather than clinical approach to animal health, which they associate with the idea of a preventive rather than curative or therapeutic approach to diseases. This conception is closely linked to the two previous points. On the one hand, it refers to the professional proximity that veterinarians maintain not only with their clients but also with technical advisors, often employees of the co-operative to which the farmer belongs or sometimes of a feed mill company. Indeed, in pig and poultry farming, technical advisors play an important role as they visit the farms much more frequently than vets. Although their role should not involve animal health issues (but rather feeding, housing or husbandry practices), they tend to conceive these aspects as a whole, as do vets when they look at the technical factors (rather than biological and medical ones) of diseases. Animal health is thus the work of a professional trio, whose theoretically distinct roles often overlap, encouraging situations of cooperation and also sometimes conflict (Adam et al. 2017). On the other hand, and consequentially, this so-called epidemiological conception of animal health establishes a form of activity which is not limited to a clinical and individual approach to pathology but which, on the contrary, opens up professional expertise to areas often considered as not specifically veterinary, such as hygiene, nutrition, zootechnics or biosecurity. The knowledge and the toolbox of industrial vets are thus extremely varied and cannot be limited to surgery tools or prescription booklets.
The veterinarians interviewed often emphasized the "evidence-based" side of their professional expertise (and legitimacy), which is based on the collection and analysis of various data and promotes intellectual stimulations regularly renewed by the diverse situations they must face. They believe all these elements to be part of preventive approaches to animal health and they tend to perceive themselves as health advisers, or "health managers" as Thoms (2015) has shown in the case of German poultry vets.
I graduated from veterinary school in 1985. Then I got a degree in epidemiology. What I realized and particularly interested me during my studies was the difference between individual medicine and herd medicine. So with my education and also my family farming background I realized that individual medicine was more a cost than a profit. So I tried to develop this kind of herd care and preventive approach. I started my professional life in rural medicine in a region that was quite a pioneer in this type of approach, especially regarding reproduction.

Poultry vet, 2015
We have audit grids and we have exploration tools. For example, I have a device to measure CO 2 , to dose carbon monoxide. I have a Ph-meter, a conductivity meter, a laser. I have a burette to measure the water flow in the feeding system. I have a light meter in case I need it. I have a camera to explore water pipes. I always have smoke bombs in my car to check ventilation in the buildings. So really, we have exploration tools in the form of scissors and gloves to perform autopsies but also equipment for managing the buildings.
But aren't you stealing work from the technicians?
Technicians do autopsies (laughs)! No, it's true that controlling ventilation doesn't mean that I am able to deal with the farm IT system. So technicians keep their skills. But I consider that it is my job to explain to the farmer that if he has a colibacillosis it's not because a germ fell from the sky but because his ventilation system doesn't work the way it should. That's part of a vet's job, even though it is the technician who is able to fix the problem. We are doing what I might call an etiological or epidemiological diagnosis.

Poultry vet, 2015
Fourthly, the characteristics of pig and poultry medicine must also be related to the type of animals with which these vets work on a daily basis. Without necessarily following the epistemological principles of the "animal turn" promoted by certain branches of the social sciences (Guillo 2015), which consists in analysing the way in which animals themselves shape human interactions or the socio-technical devices within which they take place (notion of "animal agency"), it must be noted that taking care of chickens or pigs has different implications than is the case with cattle, dogs or cats. Two elements seem central here and are directly related to the characteristics of pigs and poultry and the farming systems to which they belong. On the one hand, these animals have a relatively short lifespan: only about 30 days for a conventionally raised chicken (between 90 and 120 days in organic or label farming) and about 6 months for pigs (the sows being slaughtered after 2 or 3 years). It is therefore very different from a dairy cow that must remain healthy and productive for an average of 5 years, or pets that live 10 or 15 years, sometimes more. In these conditions, which obviously also depend on the economic structure of the agri-food industry, animal health becomes part of biological and temporal dynamics where the slightest disorder may have pathological consequences that might be seen as "just-in-time diseases", i.e. health issues directly related to, or shaped by, the sociotechnical and economic infrastructures (including the animal bodies themselves) within which they emerged (Allen and Lavau 2015). On the other hand, the health of chickens and pigs is not considered individually, but rather from a population perspective. These are animals that are reared in batches and it is the group of animals that constitutes the epidemiological reference unit for both the vet and the farmer. In certain cases, particularly in poultry farms where the production cycle is very short, many decisions are therefore made not for the current batch but for the following one, in order to avoid repetition of the same problem.
Overall, all of these aspects are typical of industrial veterinary medicine and, therefore, of this form of expertise and professional legitimacy that can be qualified as preventive veterinary medicine. To sum up, and without prejudging whether or not these elements can be found in other segments of the veterinary profession, we can say that poultry and pig medicine is characterized by (i) a vocation; (ii) a technical and preventive approach; (iii) tripartite work; (iv) animals and farming systems that "call for", or co-construct, this form of expertise.
However, when listening to veterinarians talk about their profession, this way of working on industrial farms does not seem to be linked to the rebuilding claimed by the profession since its incrimination in the AMR problem, even though certain policy measures recently implemented may encourage the development of this preventive and evidence-based approach, such as the obligation to perform antimicrobial susceptibility tests before prescribing critically important antimicrobials (Bourély et al. 2018). Indeed, the roots of this particular form of veterinary expertise can be traced back to the mid-1970s/early 1980s, when some pioneers began to specialize in pig and poultry farms-at that time during a massive industrialization and intensification process.
The origins of preventive approaches in "industrial" veterinary medicine During the 1960s and 1970s in France, particularly in Brittany and Pays de Loire, poultry and pig farms expanded rapidly (Nicourt 2013). The mixed crop-livestock model was gradually being replaced by specialist farms which were developing through a twofold process of intensification (increased herd size, confinement and containment of animals, rationalization and (bio)technicization of husbandry methods such as genetics, feeding and pharmaceuticals) and industrialization (concentration and vertical integration of the food chain's stakeholders, and taylorization of farm labour) (Diry 1985). This movement led to the emergence of new needs in terms of health management. Firstly, at that time veterinarians knew little about these animals in terms of medical knowledge or techniques (poultry and pig medicine was rarely touched upon in veterinary schools back then). Secondly, the confinement of animals in enclosed buildings led to outbreaks of disease, especially-but not only-infectious diseases which were unknown or at least whose management methods were no longer appropriate. A small number of vets then began to specialize in this type of production, viewing it as a promising and developing market.
Poultry farming was not taught at all. Poultry diseases even less so and the prevention of poultry diseases even less than that. So my gateway to poultry farming was the technical-economic approach to things, and not the purely pathological approach, which I could have done at the time on sheep or cattle. Except that there, I was in a completely capitalist system. As you can see, one can adapt to anything (laughs).

Retired poultry vet, 2018
In the pig sector, it was mainly vets employed by agricultural co-operatives who embarked on the adventure, supported in particular by the creation of the Station de Pathologie Porcine (SPP) (national veterinary laboratory specializing in pig health) in Ploufragan (North Brittany) in 1975 (Fortané 2017). In the poultry sector, which is structured more around industrial groups than around co-operatives, there were more independent vets who were orienting their practices towards this type of clientele. What these veterinarians had in common was the conviction that they could no longer do their job in the traditional way, i.e. as emergency "rural" vets, alone or in partnership in small practices serving a diversified clientele (cattle farmers and pet owners in particular, with a few occasional interventions on poultry or pig farms). Moreover, they were convinced that their job was to accompany the development of intensive and industrial livestock farming by providing services adapted to the specific animal health issues of this sector, and that their own organization had to follow and mimic the development of the industry they were working for. The term "industrial vets" therefore refers not only to the kind of clientele they were (and still are) working for, but also to their own state of mind and conception of veterinary medicine, as a profession and as a business.
In fact, we were the defenders, the propagators of intensive livestock production. We never denied that, that's what we were employed for. We were just saying "ok, but the conditions for success are this and this and this".

Retired pig vet, 2014
At the time, there was a double-digit growth in the sector, so we said: "we must stick to the development of this sector". We were focused on poultry, we had almost given up on pigs. We said: "We have to stick to the leaders". Very quickly, in 84-85, we said: "it's X [one of the biggest poultry industrial group], we'll stick to X, we'll stick to the growth of X, like a leech, we'll never let it go". That was our aim in 84-85 and it became concrete a little later when we were working for X, even though we weren't their official vets. (…) We had developed well in 84-85, so how could we consolidate our system, how could we really sell it, how could we duplicate it? This is where the notion of "global offer" began. Here, you find three activities: the veterinary practice, including advice and training, the medicines and the analysis. When you come here, you have this "global offer": the lab part, the drug part and the advisory part.

Retired poultry vet, 2018
The way this pioneering generation of pig and poultry vets worked was characterized by their capacity and interest in conceptual innovation and do-it-yourself techniques for responding to the often unknown situations they had to face. On the one hand, it concerned the development of pharmaceutical products adapted to these animals and their husbandry conditions, at a time when the pharmaceutical industry was producing very few medicines for this type of livestock, not considering it to be a worthwhile market. It was therefore not uncommon for veterinarians to order pharmaceutical raw materials (rather than manufactured drugs) in order to themselves prepare a product that could be effective in terms of both pharmacological (e.g. combining an antibiotic and an anti-inflammatory) and galenic properties (e.g. designing a drug in the form of a powder to be spread in bedding rather than to be mixed with food-for dermatological infections in particular).
In 83-84, we had outbreaks of staphylococci. Obviously, the animals caught it very early because they already started to have small pimples at 8-10 days. So the mother was a carrier, so we thought: "what if we use an antiseptic powder?" Then we thought: "we need a powder that is very powdery, that adheres well to the skin". We worked on the galenic, excipients and everything. We developed a powder and tried it and the farmers said: "this works well".
Who were you doing this with?
We made the powder ourselves. We had a mixer. In our pharmacy, we had a manufacturing workshop. (...) It was common at the time. We had the right to make extemporaneous preparations that were the result of our prescription and our imagination.

Retired pig vet, 2018
On the other hand, this broader conception of the veterinary role could be seen in the holistic vision of health that these professionals were promoting. Most of the time, they combined therapeutic intervention with bacteriological analysis and research on animal nutrition (some of these vets were also employed by feed mills). All of the larger practices of this pioneering generation developed a laboratory, sometimes a makeshift one, in order to perform the autopsies necessary for bacteriological tests.
X [a pioneering poultry vet] had a very solid clientele of horses and cattle at the time, but he was interested in birds. (...) When the first industrial poultry farms, the large flocks of 300 to 400 chickens, settled in, he moved towards that. Very quickly, he realized that the bigger the batches of poultry became, the more the diagnosis had to go through the laboratory in order to be accurate -at least regarding bacteriology and parasitology. Since he's a guy who doesn't want to do things by half, he thought: "I can't make a laboratory like that, we suck as vets, I need training". He said to his partner: "I will go to Pasteur". 11 Further education at that time was unimaginable. (...) Then, when he came back, he started his own lab and that was really the beginning of the adventure.

Retired poultry vet, 2018
In the pig sector, it is interesting to note the extent to which the history of the Ploufragan station is still rooted in the North Brittany territory, as many generations of veterinarians, still today, have done part of their training there or continue to rely on the expertise of SPP's epidemiologists or microbiologists in their daily activity (especially in the case of outbreaks of infectious diseases or to set up clinical trials).
Oh yes, with regard to emerging diseases, I often contacted the station when we saw pathologies that seemed new to us. This was the case for many diseases. There was streptococcal, there was respiratory coronavirus. At the SPP, I called [the vet in charge] and the others, they were not convinced because at that time there was still pseudorabies in the farms. I had to talk to [a pharmaceutical company] about it and then the SPP became interested.

Retired pig vet, 2014
Indeed, veterinary researchers from the SPP had developed a preventive approach to animal health called "ecopathology" that had considerable success among pig vets and farmers in the 1980s (Fortané 2017). It was mostly based on an epidemiological conception of animal health (disease outbreaks are related to multiple variables, particularly "technical", i.e. non-clinical ones), and was adapted to the specific issues and husbandry conditions of intensive livestock farming. In this regard, the type of preventive veterinary medicine which was developed at that time in France would seem to have similar characteristics to that which had flourished in the UK slightly earlier (from the 1950s to 1970s) (Woods 2013): animal health has to be conceived at the scale of the herd (and not the individual animal), be articulated to non-medical matters such as feeding, housing and genetics, and integrate the economic issues of performance and profitability within the advisory support vets provide to their clients. The most important difference during the process of institutionalizing these preventive approaches in France and UK seems to be the role played by public authorities. While in the UK, the State firmly supported the development of preventive veterinary medicine as a way to accompany the modernization of British livestock farming, in France, except for the SPP which was partially founded by local authorities and State veterinary services, most of the preventive approaches that flourished in the 1980s were supported by the private engagement of pioneering vets trying to respond to the challenges of a growing industry and, in the meantime, to capture the market of "industrial" veterinary services. 12 All in all, the point here is not to say that contemporary pig and poultry vets are nowadays using the exact same techniques and knowledge of the preventive approaches developed in the 1970s and 1980s, or even to suggest that all industrial vets of that time were doing exactly the same. 13 It is rather to demonstrate that this type of veterinary medicine has at least a 30 to 40-year history in these sectors and that some aspects of this can still be seen in the way that modern industrial vets continue to work (and to perceive their job and professional identity), such as the importance of technical expertise, epidemiological knowledge and bacterial laboratory-in other words, that a diversified form of veterinary expertise is required. Even though this history has more or less vanished from the profession's official memory, a few unconscious vestiges of it can still be seen in recent AMR debates. For example, during the campaign promoting the AMR policy in the pig sector in 2014, an implicit link was made between this pioneering medicine and the kind of expertise which is now considered to be the future of the profession and through which veterinarians tend to legitimize their role as the guardians of antibiotics. This is demonstrated by the utilization of the ecopathology icon which expresses the holistic conception of animal health (namely the so-called "Ploufragan hexagon") to promote good veterinary practices in antimicrobial use in livestock (Fig. 2).
All these elements show that what is now called preventive veterinary medicine and which is perceived, within the political context of the AMR problem, to be the model of professional expertise towards which veterinarians must turn, is not fundamentally new, at least within the knowledge and practices of pig and poultry vets. But what about the economic model on which this kind of expertise is based and how it is actually related to the use of antimicrobials?

The economic structures of professional expertise
Although it is in fine difficult to associate the development of preventive approaches with the reaction of veterinarians to their incrimination on the AMR issue, contrary to what the sole examination of professional discourses in the press or in various 12 It should also be noted that in the UK the development of preventive approaches concerned farm animal medicine in general (so mostly cattle medicine), while in France this movement was located in "industrial" pig and poultry medicine -although due to local history it would seem that in some regions other forms of preventive medicine were also developed in a small number of cattle veterinary practices as from the late 1970's (Combettes et al. 2012). 13 This is indeed the purpose of talking about "preventive approaches" in the plural, i.e. to highlight the fact that these approaches could be rooted in different local histories or individual trajectories yet still share some common principles. While pig vets were mainly referring to ecopathology by virtue of their link with the SPP, poultry vets did not use a specific term (preventive, holistic or epidemiological approach, global offer, etc.). Nevertheless all industrial vets were (and still are) referring to what they were (are) doing with the same kind of contrasting schemes: epidemiological vs clinical, preventive vs curative, herd vs individual, technical vs medical, etc. which I consider to be the common ground of "preventive approaches to animal health" with, in addition (cf. next section), an economic model based on free-of-charge services funded by drug sales and aimed at "capturing" clients.
institutional spaces relating to AMR policy-making might suggest, nowadays this model has nevertheless become more visible and contributes to the re-legitimation of the veterinary profession (Badau et al. forthcoming). However, the idea that preventive approaches will make it possible to move away from the economic model placing antibiotic sales at the heart of veterinary income seems more dubious, as the development of said preventive approaches was in fact based on this very system.

The construction of a captive market
By the notion of economic model, we mean the way in which veterinary services are monetized. One of the key elements of the criticism against veterinarians with regard to the AMR issue has been the fact that their sources of income are largely dependent on drug sales (antibiotics in particular). However, whereas professional discourses seek to explain that the development of a preventive medicine in which antibiotics no longer hold a central place will make it possible to change the "business model" of veterinary practices, it is on the contrary clear that this economic model based on drugs sales has in fact allowed these preventive approaches to flourish. Even though it is difficult to concretely assess the accountability of veterinary practices due to a lack of available data (in particular if we wish to distinguish between different segments of the

The common principles of past and present prevenƟve approaches in industrial veterinary medicine
Ploufragan hexagon: animal health is dependent on six variables (Tillon et al., 1980) 1 PrevenƟon campaign against anƟbioƟc misuse in pig farming, 2014 2 Fig. 2 The common principles of past and present preventive approaches in industrial veterinary medicine. 1-Ploufragan hexagon: animal health is dependent on six variables (Tillon 1980). This famous diagram represents the way animal health (and economic performance of the farm) is conceived within the ecopathological framework: it is correlated to six variables, namely the farmer, the animal, housing, feeding, microbes and husbandry practices. At that time, it was considered quite innovative to claim that veterinary expertise should take into consideration all these aspects and not just focusing on animals and microbes (Fortané 2017). 2-Prevention campaign against antibiotic misuse in pig farming, 2014. This poster used in the AMR campaign perfectly reproduces the six "ecopathological" variables (here referred to as the six "pillars" of animal health). It says: "No more antibiotics than needed. With my vet, I manage the health of my animals while limiting the use of antibiotics". This poster therefore illustrates how preventive approaches within veterinary medicine are deemed to be a solution to reduce antimicrobial use profession), we can nevertheless rely on estimates from various literatures. A 1984 veterinary thesis on the structure of the drug market considered that drug sales were generating 30 to 40% of the income of farm animal practices (Rivière 1984). Thirty years later, in the midst of the AMR debates, a report by the French Food, Agriculture and Rural Areas Council estimated this figure to be around 60% for farm animal vets in general, and up to 80% (including a high proportion of antibiotics) for industrial vets (Dahan et al. 2013, p. 20). So one cannot help but wonder what happened during the three last decades and to what extent this evolution of the economic model of veterinary activity has in fact supported the development of preventive approaches to animal health, contrary to the prospective narrative the profession has constructed to defend its role as the guardian of responsible use of antibiotics.
The answer is actually quite simple, and is a classic case in agricultural economics: the sale of inputs funds the advice. In the case of industrial vets, the context in the 1980s and 1990s favoured the emergence of strong competition between practices seeking to capture the growing clientele of pig and poultry farmers. This competition was also heightened by the fact that the supply of veterinary goods and services was still highly heterogeneous, due to the non-existence of standardized pharmaceutical, hygienic or nutritional products adapted to the new health problems caused by the intensification of animal husbandry. Consequently, pharmaceuticals, which are the only product on which veterinarians have a monopoly for prescribing (and therefore advice) and dispensing (and therefore sales), became the main means of ensuring client loyalty. Since the 1975 Act, the veterinary drug market has thus been developing into a captive market, where drug sales have gradually become the only monetized exchange between vets and farmers, funding all the services actually offered by veterinarians (visits, diagnosis, analyses, prescription, audit, etc.) (Bonnaud and Fortané 2018). Indeed, preventive veterinary medicine developed on the basis of this economic model which corresponded, on the one hand, to that of the few pioneering independent practices which owned bacteriology laboratories, small drug manufacturing factories or wholesale companies and, on the other hand, to that of the agricultural co-operatives which the 1975 Act established as lawful drug suppliers and which, until 2007, employed veterinary practitioners in this regard. Indeed, it was precisely by providing a whole range of "free of charge" services (visits, advice, vaccination, etc.) that these vets were able to disqualify their competitors, i.e. their "emergency" or "fire brigade" colleagues who offered little advice but were able to monetize their occasional interventions.
Yet were the farmers prepared to pay for their visits, as they would have done with rural vets at the time? Because, even the independent vets specializing in group pathologies, so pig and poultry, like those from X [one of the pioneering veterinary practices in industrial medicine], most of the time, they did not charge for their visit, their visit was paid through drug sales. In fact, most farmers were smart enough to understand that if they were satisfied with the services of a vet or a veterinary practice, they should buy drugs to pay them indirectly, even if there were price differences. I think it was like this: "You do me a favour, I need you, you treat, you prevent, we work together, I buy drugs". It was indirect payment. (…) I've always thought that the best way to ensure farmers' loyalty is to be efficient. Then they will not even talk about drug prices. There was a time when vets were very active on the farms. If farmers were happy with our services, [they didn't care about] the price of the drugs. At one point, we were a little cheaper but we weren't always cheaper. We were also known for being reasonably priced, so that there wasn't too much competition. Sure, we weren't the cheapest on the market, but that's not the issue. But I'm convinced, and I know that's the way it was for a lot of people, the best way to keep them was to meet their expectations. Their expectations were simple: "I have problems, I have to solve them, help me solve them, if it takes time ok it takes time". It was up to us to meet farmers' expectations in terms of health. If the farmer was satisfied, the rest would follow, he would take the feed, the genetics, the drugs. We had an indirect commercial role, but we could only do it if we were efficient. (…) But some of them stopped buying our drugs. They told me: "You're too expensive". So I answered: "If we are too expensive, you go elsewhere, but you no longer have the services". What does he want? A cheap price, but with a service that will be what it will be, it may be very good, ok, but the farmer is free to choose. However, he fully understood that he could not ask us to come ten times a year or twenty times and then buy zero medicines. Moreover, I tell most people: "we provide you with important services, we know that you also like to work with other people from time to time, at X for example, so don't buy everything from us, but buy a little from us, as we come to see you regularly and we need a return". And then everything was fine, they bought some elsewhere and some from us.

Retired pig vet, 2014.
The construction of a captive market is therefore a central dimension of what I call here "the economic structures of professional expertise". The development of preventive approaches within industrial veterinary medicine is not only the result of the emergence of new demands or needs from farmers, but it is also and above all the consequence of the intra-and extra-professional competition that veterinarians have had to face after the 1975 Act. Prescribing and dispensing pharmaceuticals, combined with a holistic expertise, is a means of capturing a clientele through a simple and exclusive form of economic contracting (the sale of drugs) while providing a wide range of services (diagnosis, advice, etc.). It is indeed the articulation of these two dimensions that makes it possible to prevent, or at least reduce the risk, that clients are captured by a competitor. In this sense, preventive approaches to animal health must be read simultaneously as a professional and a business model, these two dimensions being constitutive of each other. Such economic structures of veterinary expertise have been observed in another context, that of the USA in the 1950s and 1960s. Smith-Howard (2017) shows how American veterinarians, who did not have a monopoly on the sale of medicines, gradually established themselves as the main distribution channel by coupling their prescriptions with preventive services that provided real added value to farmers, compared with pharmacists or other retailers who were merely supplying the drugs. A linguistic distinction has even emerged between "dispensing" and "merchandising", contributing to (re)establishing the legitimacy of veterinarians on a new basis. In the end, it is therefore in the light of this link between professional expertise (in the sense of type of knowledge and services) and business model (in the sense of how these services are economically valorized) that we must question the current context and controversies surrounding AMR.

Towards new business models for veterinary practices?
It seems finally a little too simplistic to support the thesis of the professional conflict of interest that human health actors have mobilized to point out veterinarians' responsibility in the AMR problem, because of their dual monopoly on the prescription and supply of medicines. In fact, the structure of the veterinary drug market has set up the sale of pharmaceuticals as a quasi-unique way of making professional expertise profitable, even though this expertise was already based on preventive approaches (and thus diversified services) for quite a long time. The current challenge for the veterinary profession is therefore less the development of a preventive medicine than the renewal of the economic model on which it has been based until now. In this regard, it is actually interesting to note that the vets I met in this study clearly mention this issue and have already started to develop strategies to deal with it.
That's exactly the issue we now have with my colleague. I'm right thinking this. Historically in poultry, no charge is really made for visits. Overall, payment is made through [the purchase of] medicines, which is, for me, not a bad thing if it is not excessive. At some point, when we take the example of human medicine, you pay a doctor, you pay a pharmacist, everyone is happy because there is healthcare security. The day there's no healthcare security, I don't know what we're going to do. So that's what I explain to farmers: "The day you have to pay a vet and a pharmacist it's going to cost double". So at the moment, with this system, you only have one cost, so in theory that's good. Indeed, there can be misuse, someone who would systematically prescribe medicines even if there is no need. This is clearly an issue and it is difficult to explain, that's our problem. But honestly, every morning when I stand up, I don't say to myself: "I will prescribe 10 kilos there, 15 kilos there and 5 kilos there, and the day is done". That's absolutely not the way I think, but I can understand… and I understand when people are telling me: "Yes, but if you sell, you earn". That's all there is to say. It's been on my mind for a year.

Poultry vet, 2015
Firstly, particularly in a context where profits on medicines are now more strictly controlled (and therefore more limited), it seems essential for the veterinary profession to be able to charge for services that were previously funded by drug sales. This is the case in particular for visits, technical advice and bacteriological analyses (the latter were generally charged but often below the real cost-which could be high for the veterinary practice that has to employ laboratory technicians).
Secondly, there are strategies consisting in investing in hygiene and/or nutrition product factories (in particular disinfectants and food additives), in order to be able to sell products other than just pharmaceuticals. It is a strategy of diversification of the professional and economic activity that pioneering independent practices or certain cooperatives employing veterinarians have been using for many years, but which is now tending to become generalized among industrial vets due to the development of franchised practices.
Company X is a part of group Y [a pig co-operative]. [We sell] zero drugs. There is hygiene, so disinfectants and detergents, and also nutrition and probiotics, i.e. products that can be added to food or that farmers can put in drinking water. You have suppliers for these products or you manufacture them yourselves? There are products that we make ourselves, otherwise we choose products from everything we know, and from people we work with. We are doing clinical and zootechnical trials as well. We've written some articles, I did one on swine haemorrhagic dysentery. We did an international article in which we showed that we can prevent it with a certain probiotic. It allowed me to go and see farms in Portugal, Spain and England as well. I even went to Cuba for X. Trying to sell products?
To preach the good word! We hold meetings on a given theme, for example it might be digestive issues, and we talk about the ecopathological approach. Rather than using this or that antibiotic, there are other things that work, and we know this because we have experience with farmers from Y. Thanks to this experience, and because there were disastrous situations where antibiotics no longer worked, we can tell other veterinary colleagues [that these products work well].

Pig vet, 2014
A third strategy consists in developing various forms of contractualization with the clients. For example, the vet interviewed below tries to imagine an annual flat-rate model that would cover all veterinary services, from advice to medicines. This would be based on a form of insurance, where some farmers would ultimately pay a higher price than the actual cost of the services they have received, while others, particularly those facing more health issues, would pay a lower price (although the reverse might be true from one year to the next). In fact, this system is similar to the one set up by the so-called "veterinary groups under contract" in central and south-eastern France, but which was only developed in areas where livestock farming is not widespread i.e. in small and medium-sized cattle or sheep farms connected to local markets; this system is mainly associated with alternative agricultural projects resulting from 1970s protest movements (Combettes et al. 2012). It would be interesting to know the extent to which such a model might be easily generalizable in the heart of an industrialized agri-food system where farmers are involved in more complex and constraining chains of interdependence with upstream and downstream industries. This trend resonates with certain dynamics currently observable in the UK where farm animal vets are also concerned about the sustainability of their economic model and are trying to develop similar forms of contractual veterinary services, although less than 10% of the clientele of the practices trying to develop these kinds of contractual schemes have adopted it so far (Ruston et al. 2016). The economic model and the type of goods and services that veterinarians can provide are nevertheless now considered to be key criteria for the sustainability of veterinary businesses (Henry et al. 2016).
So I've been thinking for a year. Now I'm going to test a new system, a comprehensive flat-rate package. I'm testing it, it's brand new, with two or three farmers. This is a complete veterinary follow-up, which includes visits and medicines. We don't talk about fees anymore. [...] So I don't know yet. My package system might be good in theory, maybe not good if farmers... Because you know, on the farms, there are major variations in antimicrobial consumption. At some point the package is going to be an average. Some of the farmers will find my services a little bit too expensive whereas others will like it, but yes perhaps major antibiotic users so it might be a bad influence for them. I'm not claiming victory yet, but I'm trying to find another system.

Poultry vet, 2015
A fourth strategy consists in monetizing certain veterinary services no longer to farmers, but to co-operatives. This relates to at least two types of activity. First of all, it concerns the follow-up of "herd health plans" that co-operatives are obliged to set up if they want to be approved for selling veterinary medicines, in accordance with the 1975 Act. It is of course an activity that has existed for a long time, but that many cooperatives delegated to their employed vets until 2007. The second activity relating to this strategy is linked with the development of new roles for veterinarians, in particular those of standards controllers or certifiers. Indeed, with the development of "antibioticfree" labels, more and more co-operatives are asking vets to help them implement such standards. For example, vets have to perform "pharmaceutical audits" in an attempt to recruit farmers who can comply with such specifications, or to set up protocols to monitor antibiotic use and help farmers reduce it.
When the co-op announced its project [antibiotic-free pigs], some farmers called me the day after, or I called them, to be part of this project. So for those who were still using antibiotics in feed there were still some stages to go through but when a farmer calls you, you think that you're going to need him for the project so you have to find alternatives. You have to know if he really needs antibiotics and how to reduce them. So this is where you have to set up a procedure, to find ways to identify the flaws… and in the end this is the perfect way to familiarize farmers with this issue. (…) Well the farmer has to be motivated but this is where the bill of specifications helps, because there is an added-value. And there is also glory and self-satisfaction because the added-value isn't much, but there is both.

Pig vet, 2014
Yet these new roles for veterinarians, or more exactly these new ways of monetizing their expertise, are associated with a redefinition of their professional identity that vets are not always comfortable with. While being a health advisor rather than a clinician is perfectly in line with their preventive conception of veterinary medicine, becoming a sort of controller and sometimes even a sales representative in charge of enrolling and accompanying farmers in quality insurance schemes is not necessarily easy. This difficulty has also been noted among UK cattle vets who felt conflicted between their role of practitioner (within the framework of their relationship with their clients) and their role of "enforcers" of biosecurity practices (while implementing policy measures to prevent bovine tuberculosis), although in this case they were acting on behalf of the government and not for market schemes of private stakeholders (Enticott 2012). The vet interviewed below explains the practical and ethical adjustments he has had to make to accommodate this new way of valuing his professional expertise, and the limits he personally sets to remain in what he considers to be the role of a health advisor.
So luckily we have some experience [with following up on bills of specifications], especially with welfare standards. In this standard, the farmers have to use the co-op's animal feed. So our experience allows us to recognize the feed bags. And one day I was on a farm visit, and I saw a feed bag. And I thought "but this is not the feed from the co-op?". Because I know that it is normally small granules, and this time it was big granules. So here, clearly, the farmer is not complying with the bill of specifications! So you see, if you look carefully, you can see things. Well you can't see everything, especially when farmers don't want you to know… because in this case he even didn't think about it. Leaving the bag like that… it is sure that he's going to be in trouble with the co-op. But you won't see the ones who really want to cheat! And in this example, what happened in the end? You just let him know, asked him to be careful, and let it go? Or might you impose sanctions at some point?
Well that's not for me to decide. But the question for me is whether or not I want to report someone. One day the co-op boss asked me [about the co-op's granules] but I replied that I didn't have time to check whether every farmer in the quality insurance scheme actually uses its granules. (…) But no, what I do, mostly, is warn the farmer. I tell him that he has been found out. And I tell him that the coop is fairly meticulous and that if they find out they will take him off the scheme. So for a few months he will lose 23 cents and he won't be happy. So that's the situation. And they are absolutely capable of doing it, without any doubt. So this is what I do, I warn him but I don't think it is my role to report him, or to impose sanctions.

Pig vet, 2014
Conclusion "If I change, I may lose my clients. But if I don't change, I might lose my job"

Poultry vet, 2015
By cross-examining the development of the drug market and the professional expertise of French "industrial" vets, this article has aimed to articulate reflections from the sociology of professions and the anthropology of medicines. The AMR problem is a very interesting case-study through which to make such an articulation fruitful. Indeed, numerous works of research in the anthropology of medicines invite us to analyse drug use through the lens of the global circulation of pharmaceuticals and not just by focusing on the practices and knowledge of end-users, be they farmers or patients (Whyte et al. 2002;Petersen 2014). Antibiotic use cannot therefore be disconnected from upstream practices (or pharmaceutical "life stages", as anthropologists of medicines would call them) such as production, distribution, prescription and sale. Though little is yet known about the structure of the veterinary drug market, we have shown that as key actors at the heart of the veterinary drug chain, involved in many areas of this global circulation of pharmaceuticals, veterinarians play a major role in the regulation of antibiotic use-particularly industrial vets who are not only involved in prescription and delivery but also in some cases in production and distribution. This result is therefore interesting from the point of view of the sociology of professions as it shows that the "jurisdiction" (Abbott 1988(Abbott , 2005 of veterinarians is certainly more complex than them being mere practitioners of animal healthcare. Indeed, when we look at the veterinary profession as a whole it becomes quite obvious that veterinary knowledge is involved in many domains of animal health and food safety, from farm to policy and industry Fortané 2016, 2018). What this article shows, in addition to describing the specificities of a relatively unknown segment of the profession, is that this highly diversified form of professional expertise, and therefore the variety of areas in which it can be exerted, can also be concentrated within a tiny group of professionals, namely pig and poultry vets. Moreover, even though it still has to be more broadly demonstrated, it would seem that the trajectory of this particular segment, characterized by the development of specific approaches and economic models, is somehow quite representative and/or influential of more global changes that are now affecting the profession as a whole (at least in farm animal medicine). The expansion of the activity of industrial vets within the drug chain and the "technical" domains of livestock farming indeed corresponds to a (conflicted) evolution of veterinary jurisdictions that echoes the profession's ongoing attempts to renew itself in the context of AMR controversies. All in all, articulating reflections from the sociology of professions and the anthropology of medicines has allowed us to highlight the "economic structures of professional expertise" (that is to say that professional practices and knowledge are shaped by the way they are economically valued on a given market), which is of considerable importance for the analysis of the AMR problem.
Indeed, it underlines the fact that AMR cannot be addressed by simply focusing on antibiotic use or prescription and, more importantly, it shows that the framing of the AMR problem cannot be understood without reconnecting it with the global transformations of the veterinary profession (both in terms of expertise and business model). In this regard, we have shown that the veterinary profession has developed a prospective narrative to defend its role as the guardian of antibiotics (i.e. moving towards preventive approaches in order to prescribe less antibiotics and be less dependent on drug sales) that does not fully correspond to either the history or the actual practices of industrial vets. Such approaches have in fact already existed for many years and it is thanks to this diversified veterinary expertise that this segment of the profession has developed. However, the economic model upon which this expertise has been based is in fact the one which is nowadays criticized within AMR controversies (i.e. the sustainability of veterinary businesses being highly dependent on antibiotic sales), so that it would appear relatively doubtful, if not ironic, that the profession now brandishes preventive approaches as the undisputable solution to antibiotic overuse or misuse. Indeed, drug sales were almost the only way of monetizing veterinary services in the pig and poultry farming sectors and this is how the pioneering generation of industrial vets disqualified their traditional competitors, by "enrolling" clients through the development of a captive drug market. So although promoting preventive approaches is probably the right way to encourage veterinarians to move towards a "health advisor" role where antibiotics are neither the main tool of their activity nor the main source of their income, the profession still has to develop economic models (practice accountability, contractual arrangements with the clients, etc.) that do not rely on drug sales. Recent policy measures and incentives are probably pushing in this direction, as are broader trends such as the development of franchised practices and new professional roles and status, but this still has to be empirically confirmed (even though we know that over recent years antibiotic use has already started to decrease). Social sciences have a major role to play here, by being theoretically and methodologically innovative in order to grasp complex and entangled dynamics to which the AMR problem has just begun to draw attention.

Compliance with ethical standards
Conflict of interest The author declares no competing interests.


provenance: AgriculturalAndFoodSciences,2019-2020-Agricultural And Food Sciences-2020.jsonl.gz.gz

text:
Antifungal Activity of Essential Oil and Main Components from Mentha pulegium Growing Wild on the Chilean Central Coast

: Fungal diseases, both pre- and post-harvest, are currently difficult to control—increased antifungal resistances have further stimulated the search for natural alternatives. The objective of the present research work was to evaluate the antifungal activities of Mentha pulegium essential oil (EO) and its major constituents. The EO was obtained from hydro distillation of fresh leaves, and composition was determined using gas chromatography/mass spectrometry (GC/MS). The main components were identified as pulegone (29.33%), menthol (28.79%), menthone (20.48%), and isopulegol (9.75%). EO and isopulegol exhibited the highest antifungal activity, with half maximal effective concentrations (EC 50 ) inhibiting mycelial activity of Monilinia fructicola at 24.6 µg/mL and 20.8 µg/mL, respectively, and against Botrytis cinerea , at 301.45 µg/mL and 333.84 µg/mL, respectively. These findings could lay the foundation for developing antifungal agents of agricultural value.

Introduction
Phytopathogenic fungi are a serious threat to plant health, causing a plethora of diseases that contribute substantially to overall losses in agricultural yield [1]. In addition, fungal plant pathogens are divided into two main groups: biotrophic pathogens, which form intimate interactions with plants and can persist in and utilize living tissues (biotrophs), and necrotrophic pathogens, which kill the tissue to extract nutrients (necrotrophs) [2]. Botrytis cinerea and Monilinia fructicola are the best examples of broad-host-range, necrotrophic plant pathogens. The success of these pathogens on diverse crops is attributed to the production of an extensive array of compounds, enzymes, and toxins, which singly or in combination likely interfere with common structural and functional features shared among different plant families [3]. For instance, B. cinerea can infect more than 235 different plant species prevalent over geographically diverse regions, causing grey mould [4], while M. fructicola can infect different members of the Rosaceae family worldwide, causing brown rot [5]. The losses generated by these fungi have led farmers to combat them mainly with chemical treatments, which cause harmful effects on human and environmental health, and may result in more resistant strains and an increase in production costs [6]. Recently, many researchers have demonstrated that essential oils (EOs), due to their effectiveness, low toxicity, and low persistence in the environment, should be used as a promising form of alternative to synthetic fungicides [7].
Mentha pulegium L. (known in Chile as poleo) is a medicinal flowering plant native to Chile, Europe, North Africa, and the Middle East [8] that grows wild in the temperate regions. Traditionally, Mentha pulegium has been extensively utilized in traditional herbal medicine to treat several ailments, including uses as a stomachic, expectorant, diuretic, menstrual treatment, and microbial infections [9]. Various biological properties for Mentha pulegium have been described, including antioxidant, antitumor, insecticidal, and antimicrobial activities [10][11][12]. The constituents of Mentha pulegium oil have been the subject of numerous studies, which have shown differences in its composition depending on the region of cultivation [13,14].
Based on these considerations, the aim of this communication was to determine by means of in vitro antifungal activity bioassays the effectiveness of the Mentha pulegium EO and its chemical constituents against some important post-harvest diseases, such as Botrytis cinerea and Monilinia fructicola. The motivation behind this study is the wish to have natural substances that are active for possible organic control against the deterioration of fruits.

Chemicals
Pulegone, menthol, menthone, and isopulegol were obtained from Sigma-Aldrich, Darmstadt, Germany. All other chemicals used were analytical grade and obtained from either Sigma-Aldrich or Merck.

Plant Material
Leaves of Mentha pulegium were randomly selected and collected in March 2019 from the locality Laguna Verde, Valparaíso, Chile (33°03′04″ S, 71°39′34″ O). The sample was stored as a voucher specimen MP2019-3.24 at the Natural Products Laboratory, University of Playa Ancha.

EO Extraction and Analysis
Essential oil was extracted from 500 g of fresh plant for 6 h by hydro distillation (3.0 L, H2O) in a Clevenger-type apparatus. The EO was dried over anhydrous sodium sulfate and stored at −20 °C until analysis. The EO component analysis was performed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) using the instrumentation described below. A Thermo Scientific GC-MS system (GC: model Trace GC Ultra and MS: model ISQ, Thermo Fisher Scientific, Waltham, Massachusetts, USA) was used for the analysis of the sample. The separation was performed on a 60 m × 0.25 mm internal diameter fused silica capillary column coated with 0.25 µm film Rtx-5MS. The operating conditions were as follows: on-column injection; injector temperature, 250 °C; detector temperature, 280 °C; carrier gas, He at 1.25 mL/min; oven temperature program: 40 °C for 5 min, increase to 260 °C at 5 °C/min, and then 260 °C for 5 min. The mass detector ionization employed an electron impact of 70 eV. Recording conditions employed a scan time of 1.5 s and a mass range of 40 to 400 amu. Compounds in the chromatograms were identified by comparison of their mass spectra with those in the NIST/EPA/NIH Mass spectral Library (2008), and by comparison of their retention index with those reported in the literature [15], for the same type of column or those of commercial standards (Sigma-Aldrich, Darmstadt, Germany).

Fungal Isolates
The isolates of B. cinerea and M. fructicola were isolated directly from grape and peach sick fruits, respectively. The isolates were maintained on potato dextrose agar (PDA) medium in the dark at 22 °C. Fungal isolates were characterized based on their morphology. It is part of the collection of microorganisms of the Laboratory of Biological Tests of the Department of Chemistry of the University Technical Federico Santa María.

In vitro Antifungal Activity
The antifungal activity of the Mentha pulegium EO and main compounds against B. cinerea and M. fructicola were tested according to a procedure previously reported [16] using radial growth rate assay in potato dextrose agar (PDA) growth [16]. Fungal inocula of B. cinerea and M. fructicola were prepared in plates with potato dextrose agar (PDA), incubated for 3 days and 1 week, respectively, at 25 °C. The oil and main compounds were dissolved in an ethanol/water solution (5% v/v) and were added to a Petri dish containing PDA medium (5 mL). The final tested concentrations were 10, 25, 50, 150, and 250 µg/mL for each treatment. PDA medium containing 1% ethanol was considered as the negative control (C − ), whereas BC-1000 ® and Mystic ® 520 SC, commercial fungicides (CHEMIE ® , BAYER, Santiago, Chile), were used as the positive control (C + ) at the same concentrations and under the same bioassay conditions. A mycelium agar disk (4 mm in diameter) of the pathogen's fungi were placed in the center of the PDA plates. B. cinerea was incubated for 3 days at 23 °C, whereas M. fructicola was incubated for 1 week at the same temperature in the dark. Each treatment was replicated three times. The inhibition percentages of mycelial growth for each compound were calculated and compared with the negative control as described in a previous report [17]. The effective concentration that inhibited mycelium growth by 50% (EC50) was obtained for each treatment by fitting % Inhibition and concentration to a dose-response equation. The fit analysis was performed using the Origin 8.0 software [17].

Statistical Analysis
The data of Table S1 of the supplementary material and EC50 of Mentha pulegium EO and its main compounds were reported as the mean values ± standard deviation (SD). The inhibition percentages of mycelial growth for oil and each compound were calculated and compared with the negative control as described in a previous report [16]. An analysis of variance (ANOVA) was performed on all data with a post-hoc Tukey HSD (p ≤ 0.05).

Essential Oil Composition
Hydrodistillation of Mentha pulegium aerial parts gave yellow EO in 1.30% yield (w/w). The chemical composition of the EO is given in Table 1.
The chemical composition of the Mentha pulegium EOs has been the subject of numerous studies, which show significant differences [13,18]. However, the chemical profile of the volatile oil of the studied plant was drastically different than previously reported. In our study, the oil of Mentha pulegium was found to contain equivalent amounts of pulegone and menthol. It is likely that these chemical variations are due to the diverse climatic and geographical differences between Mentha pulegium wild habitats in different countries as well as the divergent genetic potential of plants for compartmentalization of different biochemical pathways leading to a wide variety of oil components [19].    The effect on mycelial growth is evaluated by comparing the growth areas with that observed for the negative control. The results are expressed as a percentage of inhibition, which is calculated as the ratio of the area of B. cinerea and M. fructicola in the presence and absence of the Mentha pulegium EO and main compounds, and they are summarized in Table S1 (Supplementary Material). In addition, EC50 values (concentration causing 50% inhibition of mycelial growth) were obtained from fitting data to a dose-response curve. The comparison between antifungal effects of EO and natural compounds were further confirmed by comparing their effective concentrations for EC50 listed in Table 2.  On the other hand, this is the first report of isopulegol activity against these phytopathogenic fungi. Furthermore, the results indicate that menthone and pulegone presented slight inhibitory effects on B. cinerea, in accordance with previous reports [20,21]. Menthol showed inhibitory effects on both, although with a slightly higher inhibition against M. fructicola, which agrees with previous findings [21][22][23]. In general, the results of the inhibitory effect of Mentha pulegium oil is consistent with previous reports on the antifungal activity of essential oils, which showed that the inhibitory effects of essential oils tend to increase according to their major component as follows: phenols > alcohols > aldehydes > ketones > ethers > hydrocarbons [24]. Numerous literature studies have provided support for the antimicrobial activities of several of the compounds in Mentha pulegium oil [25]. For instance, terpene alcohols (isopulegol and menthol) have shown greater inhibition of mycelial growth than have terpene ketones (menthone and pulegone) against both pathogens. Moreover, other authors have similarly found that terpene alcohols tend to be relatively active, regardless of structural types, as a function of their hydrogen capacity and water solubility [26].

In Vitro Antifungal Activity of the EO and Main Compounds on Mycelium Growth of
The research team expects to expand upon the most active compounds found in this study, which are promising candidates for structural modification, as starting materials for potent antifungal hybrids against globally important agricultural pathogens.

Conclusions
Overall, the evaluation of Mentha pulegium EO against the two most important fruit diseases responsible for the most post-harvest losses revealed that the essential oil and its main components were moderate growth inhibitors. Activities toward the two pathogens differed slightly, with higher inhibition against M. fructicola. Our results suggest that the antifungal activity of the oil is due to the synergistic effect of the components, including some not examined thus far. In turn, Mentha pulegium oil may be potentially used in natural therapies to treat infectious diseases in plants, and its inhibitory abilities help confirm the ability of some terpenes-in our case, isopulegol and menthol-to inhibit M. fructicola at low concentrations. In addition, we highlighted that mixtures of isopulegol and menthol could represent a promising starting point for the development of antifungal agents. Combinations of these two substances in various ratios should be studied, and it will be enlightening to evaluate the interaction effects of either or both isopulegol and menthol with commercial fungicide.
Supplementary Materials: The following are available online at www.mdpi.com/xxx/s1, Table S1. Effect of on in vitro mycelial growth of B. cinerea and M. fructicola measured as a percentage of inhibition