extfieldsofstudy:
Materials Science
Medicine

s2fieldsofstudy:
Chemistry
Materials Science

text:
Multistimuli Responsive Organogels Based on a Reactive Azobenzene Gelator

A reactive azobenzene based super organogelator was found to rapidly and reversibly transform a range of hydrophobic solvents from gels to solutions upon changes in temperature, light and shear force. More specifically they formed gels at concentrations as low as 0.08 wt%. Upon heating, exposure to UV light, or application of shear, the π-π bonding was disrupted which resulted in a rapid drop of both modulus and viscosity. This was confirmed by (1)H NMR, SAXS, and rheological measurements. Although many examples of organogelators are known in the literature, this is the first time that a reactive group, a benzoyl chloride, has been incorporated in a supramolecular organogel structure. Moreover, this group is available for subsequent synthetic modifications. The presence of benzoyl chloride groups showed a remarkable effect on the formation and properties of the gels. Compared with other approaches, this strategy is advantageous in terms of structural design since it not only produces a multi-responsive soft material but also allows facile modifications which may expand the applications of organogels to other fields.

Introduction
Organogels are capable of forming gels with organic liquids at relatively low concentrations through non-covalent interactions. They differ from traditional polymer gels which typically form three-dimensional network structures through covalent crosslinks. 1 Low molecular weight organogels (LMOGs) may be thermoreversible, viscoelastic materials which can selfassemble into complex three-dimensional structures via multiple non-covalent interactions including hydrogen bonds, hydrophobic interactions, p-p interactions and van der Waals forces. [2][3][4] Responsive or 'smart' LMOGs are new types of selfassembled materials which have attracted great interest recently. 5,6 They can dynamically respond to external stimuli and, thus, are of particular interest in both research and industrial applications such as controlled release systems, catalyst systems, and nano-reactors. 7,8 Several different types of smart responsive systems have been reported, namely, photo-, electrochemical, ionic and pH responsive LMOG systems. 9 Among these external stimuli, light has attracted considerable attention, since it is a non-contaminating stimulus that provides a broad range of tunable parameters, e.g., wavelength, intensity, and duration, to manipulate the rheological properties of photoresponsive systems. [10][11][12] Photo-active groups such as diarylethene substituted butadienes, 13 Fe(II) triazoles, 14 merocyanines, 15 and azobenzenes [16][17][18] have been successfully introduced to LMOGs. Through these groups the chemical nature of the gel can change the selfassembly structure upon exposure to different wavelengths of light. LMOGs incorporating the azobenzene moiety exhibit trans-to-cis photoisomerisation behavior which inuences the mechanical properties of the gel upon UV/visible light irradiation. 19,20 Recently, multistimuli responsive organogels containing azobenzene have also been reported. For example, Liu 21 designed and synthesized an organogelator (Azo-G3) whose selfassembly behavior and gel formation ability was investigated in detail. Meanwhile, Wang 22 and Zhang 23 designed and studied multistimuli responsive organogels featuring electroactive tetrathiafulvalene (TTF) and photoresponsive azobenzene groups. Although azobenzene gelators containing relatively inert functional groups, such as alkyl chains, benzene rings, ethers and esters, have been widely reported, [24][25][26] to the best of our knowledge, multistimuli-responsive organogelators comprising reactive functional groups which are capable of forming stable gels have not been previously described. Typically, LMOGs form gels at concentrations higher than 1.0 wt% which may limit their commercial applicability. Zinic et al., dened 'super gelator' as a class of gelators with critical gelation concentration (CGC) below 0.1 wt%. 27 To date relatively few azobenzene based super gelators have been reported. 8,21,27 Reactive groups which are incorporated into LMOGs not only act as an effective unit to control the morphology of their selfassembly but also play an important role in forming an organogel. More importantly, they may provide a bridge to introduce further functionalities through synthetic elaboration and expand the applications of such smart materials. [28][29][30] With those objectives in mind, we designed and synthesized a new low molecular weight organogelator (C 8 -Azo-TPC), composed of an alkyl chain, an azobenzene unit, and a reactive benzoyl chloride moiety. The azobenzene unit provides the possibility of multiresponsiveness while the benzoyl chloride moiety enables facile functionalization of the gelator.

Experimental section
Synthesis of C 8 -Azo-TPC, preparation, and characterization are described in detail in the ESI. † All chemicals were purchased from Sigma-Aldrich and Merck and used without further puri-cation. No purication was performed on the solvents except for the tetrahydrofuran (THF) and toluene, which was taken from an Innovative Technologies, Inc., solvent purication system. All the chemicals were characterized by 1 H and 13 C NMR spectroscopy on a 400 MHz Bruker Ultrashiled Spectrometer (Bruker, Germany) in CDCl 3 . Accurate mass spectra were obtained on a Thermo Scientic Q Exactive FTMS, employing ASAP probe. Photoisomerization of C 8 -Azo-TPC was measured on a Cary 50-Bio UV-Vis Spectrophotometer (Varian) against a background of solvent in a quartz cuvette. Trans to cis isomerisation was induced by an EXFO Acticure 4000 light source via a liquid light-guide working at 365 nm wavelength. The UV light intensity was about 3.8 mW cm À2 . Cis to trans isomerisation was induced by visible light at 12.1 mW cm À2 . UV-vis data was collected every 30 seconds. Rheology was conducted using an ARES photo-rheometer (TA Instruments, USA) connected to an EXFO Acticure 4000 UV light source (365 nm, 200 mW cm À2 ) and quartz-halogen visible light source (>540 nm, 38 mW cm À2 ) via liquid light-guide. A Peltier temperature controller was also connected to the rheometer to maintain the temperature. POM was conducted using a Nikon Eclipse 80i. SAXS experiments were performed at the Australian Synchrotron on the small/wide angle X-ray scattering beamline working at 12.0 keV.

Determination of critical gelation concentration (CGC)
Typically, 5 mg of gelator was mixed with 0.5 g of solvent. The mixture was heated until the organogelator dissolved and a clear solution was obtained. The solutions were then cooled to room temperature and gel formation was determined by inverting the vials.

Results and discussion
The synthesis of the 4-chlorocarbonyl-benzoic acid 4 0 -(4 00 -octyloxy-phenylazo)-benzyl ester (C 8 -Azo-TPC, (4)) started from phenol and p-ethylcarboxylate analine in a 4 step procedure outlines in Scheme 1. The synthetic details and characterization data are provided in the ESI. † The gelation ability of C 8 -Azo-TPC was evaluated in various organic solvents by inversion test of cooled solutions. The gelation results with critical gelation concentrations are presented in Table 1. Stable gels were formed when the C 8 -Azo-TPC was mixed with nonpolar solvents such as alkanes while it was soluble in polar solvents, such as chloroform, acetone and THF. Apart from gels formed in DMF and petroleum fuel, C 8 -Azo-TPC was able to form gels at concentrations less than 0.1 wt%, indicating its 'super gelator' properties. 31-33 C 8 -Azo-TPC formed a translucent yellow gel in alkane or mixed solvents at very low concentrations with CGCs as low as 0.08 wt%, directly comparable to the best know azobenzene gelators. 8,21 Fig. 1 shows photographs of the azobenzene sol (60 C) and gel (20 C) in n-dodecane at 0.1 wt%. Aer cooling, it formed a light transparent yellow gel, through which the background could be clearly observed (Fig. 1). The intensity of the color of the gels and solution depended on the concentration of the C 8 -Azo-TPC used.

Thermal induced sol/gel transitions
Representative photographs of sol-gel process are shown in Fig. 2. An orange colored solution in n-dodecane (1.0 wt%) was formed by heating. Upon cooling, the yellow colored gel could be regenerated within 3 minutes (or faster if actively cooled) ( Fig. S12 †). This reversible gel-sol phase transition could be repeated several times as demonstrated by the reversible changes in optical transmission upon repeated heating and cooling cycles between 20 and 60 C (Fig. 3). Even aer several months, the gel remained stable. A rheological study showed a reversible change in viscosity with change in temperature (Fig. 4). Initially, at 20 C the sample was a viscous liquid with a |h*| $ 210 Pa s. immediately following the increase in temperature, a dramatic decrease of viscosity was observed. Specically, the viscosity reached a new steady state of |h*| $ 5 Pa s at 55 C which was maintained until the temperature began Table 1 Gelation ability of C 8 -Azo-TPC in various solvents at room temperature a

Solvent
State a CGC, critical gelation concentration, the minimum concentration necessary for gelation of solvents; S, soluble; G, gel; PG, partial gel. to decrease. Upon a reduction in temperature, the viscosity was restored to the initial state, and the sample regained a gel-like viscous state. Fig. 5 shows 200Â magnication polarizing optical microscope (POM) photographs of the C 8 -Azo-TPC gel in n-dodecane at 1.0 wt%. The structure of both the gel (20 C) and solution (80 C) could clearly be observed. Bright areas in the POM images indicate the presence of a self-assembled macroscale C 8 -Azo-TPC domain potentially a liquid crystal like structure. Upon heating the polarization phenomenon mostly disappeared due to the loss of an organized network structure. Jung et al. reported similar behavior of organo gels, which were formed through intermolecular hydrogen bonds, p-p stacking, and hydrophobic interaction. 34 Variable temperature NMR spectroscopy was performed to investigate the gelation mechanism. In CDCl 3 , a non-gelling solvent for C 8 -Azo-TPC, the 1 H NMR the spectrum of C 8 -Azo-TPC contains only one set of peaks consistent with the proposed structure (Fig. S4 †). In contrast, the 1 H NMR spectrum of C 8 -Azo-TPC in cyclohexane show multiple sets of peaks consistent with the coexistence of C 8 -Azo-TPC in two different states: an aggregate (peaks at d7.58, 6.94, 6.76 5.42 and 3.92 ppm) and single molecular state (peaks at d8.41, 8.12, 7.74, 7.08, 5.50 and 4.02 ppm) (Fig. 6). The difference in chemical shi between protons in the gel and solution state are consistent with molecules associated by p-p stacking and/or the hydrogen-bonding interactions and those of the free molecules, as also observed by Jung. 34 Upon heating, the integral of the aggregate peaks decreased while the integral of the single molecular state peaks increased. Upon cooling, the reverse changes in peak integrals were observed. Although the integral of each peak changed depending on the temperature, the integration ratio between the aggregate and single molecule state was constant. The    integration ratio of peaks at d3.90-4.10, 5.35-5.60, and 6.70-8.50 ppm corresponding to the alkyl chain methylene (h), benzyl methylene (c) and aromatic protons (a, b, e, d, g, f), respectively, remained at 1 : 1 : 6 at all temperatures. Thus, the changes in the NMR spectrum with temperature are consistent with a gelator transitioning between the gel and solution state resulting from variation in the interactions between the molecules. Synchrotron small angle X-ray scattering (SAXS) is a powerful method for exploring supramolecular structures. 35 Further evidence for the thermally driven dissociation of the self assembled gels were obtained by SAXS analysis of the C 8 -Azo-TPC gels. Scattering peaks in the SAXS spectrum of C 8 -Azo-TPC gel in n-dodecane 1.0 wt% at room temperature at q ¼ 0.13Å À1 and q ¼ 0.21Å À1 show the presence of a long-range ordered structure (Fig. S15 †). However, this long-range order decreased as the sample was heated with the structure almost completely disappeared when the mixture was heated to 85 C.

Shear induced sol/gel transitions
In addition to temperature, the gel-sol phase transition could also be triggered by mechanical stimulus. For example, as shown in Fig. 2, at 1.0 wt% concentration, the gel of C 8 -Azo-TPC in n-dodecane became uid as a result of vigorous shaking. This is consistent with partial disruption of the self-assembled structure. In this system, as indicated by the 1 H NMR analysis above, C 8 -Azo-TPC co-existed in both gel and solution at the same time. In order to obtain a visual insight into the morphologies, optical microscopy (OM) was used to observe the gel-sol phase transition (Fig. 7). In the gel state, as shown in Fig. 7a, the gel exhibited entangled and dense brous aggregates. Three dimensional brous networks containing bers with widths of several micrometres and lengths of hundreds of micrometres were observed for the gel state. Similar brous structures were also observed in other azobenzene containing gels. 21,19 These three dimensional networks, formed from self assembly of the C 8 -Azo-TPC into a brous structure, were responsible for the gelation. By applying force to the gel between microscope slides, the generated shear caused the gel to transform into vesicular structures (Fig. 7c). Following exposure to shear, Lee et al., also reported observing similar changes to the gel morphologies. 36 These observations imply that without shear C 8 -Azo-TPC tended to aggregate into linear bers due to p-p interactions but formed vesicular structures under mechanical stimuli which overcame the relatively weak p-p interactions. Thus, the major reason why the system acted as a uid gel-sol mixture was associated with its aggregation mode. Fig. 7b shows the transition state between gel and sol which has not previously been reported. Owing to the mechanical stimuli, the brous aggregate was partially broken resulting in a mixture of both brous and vesicular structures. Aer resting for a few minutes, the micelles disappear and long microbers slowly reformed. Regeneration of the bers resulted in the reformation the gel state. The cycle involving vesicle-ber transformation could be repeated many times (Fig. 2).
The mechano-responsive behavior of the organogels reported here was further investigated by rheological measurements. Plots of storage (G 0 ) and loss (G 00 ) moduli monitored as functions of shear strain were shown in Fig. 8. It can be clearly observed that at initial strain values, the value of G 0 (about 4600 Pa) was greater than G 00 (about 1300 Pa), indicating a dominant elastic characteristic of the sample. When the strain was initiated, the G 0 and G 00 values of the gels decreased with increasing strain at 20 C. At low strain values, a sharp decrease of G 0 and G 00 was observed, representing a partial breakup of the gel. Furthermore, a pronounced reduction was recorded for the storage modulus (G 0 ), which dropped from around 4800 Pa to 600 Pa. Specically, the viscosity plummeted from about 1000 Pa s down to around 150 Pa s. Likewise a reduction was recorded for the loss modulus (G 00 ), which dropped from around 1400 Pa to 500 Pa.  Frequency sweep experiments were conducted to study the mechanical properties of the gel. In all cases, the value of G 00 was below the value of G 0 at all frequencies. Furthermore, the G 0 and G 00 of both gels exhibited very strong frequency dependence in the range from 0.1 to 10 rad s À1 and minimal frequency dependence in the frequency range of 10-100 rad s À1 . The results depicted in Fig. 9 showed that a pronounced increased was recorded for the storage modulus (G 0 ), which increased from around 2600 Pa to 6000 Pa, as well as the loss modulus (G 00 ), which increased from around 1500 Pa to 4600 Pa. Specifically, the viscosity dropped markedly from about 2000 Pa s to around 10 Pa s. Similar thixotropic properties of the organogels in other solvents were also observed (e.g., see n-heptane results in the ESI section †).

Photo induced sol/gel transitions
Azobenzene compounds are well-known for their reversible photoisomerization between the trans and cis forms under alternating UV and visible light irradiation. 37 In order to investigate the light-responsive behavior of the C 8 -Azo-TPC in n-dodecane, UV/Vis spectroscopic studies of the compound were also performed. C 8 -Azo-TPC shows a broad absorption around 350 nm mainly due to the trans form of the azobenzene group. The resulting gels were subjected to UV irradiation at 365 nm for 20 min at 20 C. The orange gels were found to collapse gradually and nally turned into transparent solutions. As shown in Fig. 10(a), UV irradiation results in the trans-cis isomerization, indicated by the decay of the maximum absorption band at around 350 nm and the generation of a new absorption at 446 nm. Importantly, for practical applications, this photo-induced isomerization was reversible, as the azobenzene in cis form relaxes to trans form within a few minutes aer exposure to visible light or more slowly in the dark. Fig. 10(b) showed that the cis to trans isomerisation was achieved by the subsequent irradiation of visible light.
Following a similar trend to the viscosity measurements, the dynamic changes to the storage modulus (G 0 ) and the loss modulus (G 00 ) with time were clearly observed under UV light exposure. Initially in the absence of UV exposure, the G 0 was greater than G 00 indicating solid-like gel (i.e. beyond the G 0 , G 00 crossover point). However, during exposure of the gel to UV light (from 3 min) a dramatic decrease of modulus was observed and a new plateau value was reached within 1 min (Fig. S13 †). A corresponding change in viscosity was also observed (Fig. 11). The initial highly viscous gel changed to a low viscosity uid under UV irradiation within one minute. Aer a period of UV exposure, a new steady state was established and the UV light was turned off. At the same time, visible light was turned on. The reversibility of the system was demonstrated by the rapid recovery of the rheological properties upon exposure to visible light. Viscosity was restored close to the initial state before UV light irradiation recommenced, and the sample regained a gellike viscous consistency. Such gel-sol phase transition by UV and visible light irradiation was reversible and again could be repeated many times (Fig. 2).
Real-time synchrotron SAXS measurements 38,39 were also carried out to obtain structural information of the gelator in ndodecane under different light exposure (Fig. 12). Initially, the SAXS spectrum of the gel was measured in the dark. Upon exposure to UV light, the SAXS peaks at q ¼ 0.21Å À1 disappeared and peaks at q < 0.10Å À1 decreased signicantly in intensity. When the UV light was switched off and the visible  light turned on immediately, all the scattering peaks which disappeared under UV light re-appeared, indicating a reversible structural rearrangement. The rst ve diffraction peaks (q < 0.10Å À1 ) indicated the formation of ber aggregation in the gel and along with the POM measurements strongly suggest a liquid crystal like structure ( Fig. 12 and S16 †). 40 Moreover, multiple cycles of this switching response can be achieved, demonstrating the reversibility of self-assembly in the system. The real time SAXS results conrmed that the changes in bulk properties result from the system transitioning between gel and sol states as the self-assembled structure forms and dissociates.
Derivatives of C 8 -Azo-TPC C 8 -Azo-TPC contains a reactive group, an acyl chloride, which can remain active in the gel and opens the door to further synthetic elaborations (Scheme 2). In principle, the acyl chloride of C 8 -Azo-TPC can react with a number of nucleophiles enabling a large number of derivatives to be prepared with the potential to introduce many functionalities. For example, C 8 -Azo-TPC is a hydrophobic segment, which can react with hydrophilic polyethylene glycol methyl ether (MPEG) to form amphiphilic surfactants (Fig. S7 †). These surfactants have similar multi-stimuli responsiveness with C 8 -Azo-TPC and can form a gel in some organic solvents. Not all compounds generated by reaction with C 8 -Azo-TPC with nucleophiles have multiple responsive behavior. For instance, the product from the reaction of C 8 -Azo-TPC with ethanol or water did not form a gel in organic solvents as shown in the Table 2. This may be because the products disrupt the appropriate intermolecular forces needed to form a gel. Carboxylic acid and ester groups may break the multiple non-covalent interactions such as hydrogen bonding, donor-acceptor and hydrophobic interactions between organic gels, thus losing the possibility of gelation.

Conclusions
We have prepared a highly efficient and reactive super gelator containing an azobenzene group and studied its gel-forming properties. We found that some of these systems had good gelation properties in non-polar alkyl solvents due to reversible non-covalent self-assembly of C 8 -Azo-TPC into brous structures. The lowest CGCs can approach 0.08 wt% indicating that C 8 -Azo-TPC is a super gelator. This organogel exhibited multiple stimuli-responsive behaviors upon exposure to a number of environmental stimuli including temperature, light, and shear. Upon irradiation with UV light, the trans form of the C 8 -Azo-TPC rapidly photoisomerizes to its cis form resulting in rapid disruption of the gel. Application of heat or shear also resulted in sol state through disruption of the non-covalent interactions between the C 8 -Azo-TPC molecules. An insight into the morphologies of sol-gel transition was obtained by using optical microscopic images, variable temperature 1 H NMR spectroscopy and SAXS analysis. Importantly, C 8 -Azo-TPC may have many potential applications owing to its in-built active reactive group (benzoyl chloride). Adducts of C 8 -Azo-TPC with PEGs were also able to form gels in non-polar alkyl solvents.
Through reaction of C 8 -Azo-TPC with a range of nucleophiles, a wide range of functionalities may be introduced opening the door to new applications for these active materials. The ability to control physical properties by external stimulus such as light, heat and shear may allow such smart materials to be used in a range of applications, such as hardeners of solvents and sensors.


extfieldsofstudy: Chemistry Medicine

text:
Total Synthesis of Viridicatumtoxin B and Analogues Thereof: Strategy Evolution, Structural Revision, and Biological Evaluation

The details of the total synthesis of viridicatumtoxin B (1) are described. Initial synthetic strategies toward this intriguing tetracycline antibiotic resulted in the development of key alkylation and Lewis acid-mediated spirocyclization reactions to form the hindered EF spirojunction, as well as Michael–Dieckmann reactions to set the A and C rings. The use of an aromatic A-ring substrate, however, was found to be unsuitable for the introduction of the requisite hydroxyl groups at carbons 4a and 12a. Applying these previous tactics, we developed stepwise approaches to oxidize carbons 12a and 4a based on enol- and enolate-based oxidations, respectively, the latter of which was accomplished after systematic investigations that revealed critical reactivity patterns. The herein described synthetic strategy resulted in the total synthesis of viridicatumtoxin B (1), which, in turn, formed the basis for the revision of its originally assigned structure. The developed chemistry facilitated the synthesis of a series of viridicatumtoxin analogues, which were evaluated against Gram-positive and Gram-negative bacterial strains, including drug-resistant pathogens, revealing the first structure–activity relationships within this structural type.

■ INTRODUCTION
Within the class of tetracycline antibiotics, viridicatumtoxin B (1), 1 viridicatumtoxin A (2), 2 and spirohexaline (3) 3 (Chart 1) are unique in that they include in their structures a geranylderived subunit in the form of a spirobicyclic system (ring system EF). In contrast to the majority of tetracyclines, these members of the group are also distinguished by their fungal, rather than bacterial, origins. The subject of this article is the pursuit of viridicatumtoxin B (1) by total synthesis, its full structural elucidation, and investigation of its antibacterial properties as well as those of selected synthetic analogues. The following brief historical overview places the present work and its aims in perspective within the field of tetracycline antibiotics.
Due to their complex structures and important biological activities, tetracyclines have been the subject of numerous synthetic campaigns since the 1950s. Noteworthy achievements in tetracycline synthesis include those recorded by Woodward/ Pfizer, 13 Shemyakin, 14 Muxfeldt, 15 Barton, 16 Wasserman/ Scott, 17 Stork, 18 Tatsuta, 19 and, more recently, Myers 20 and Evans. 21 First isolated in 1973 from a Penicillium strain in South Africa, viridicatumtoxin A (2) yielded to X-ray crystallographic analysis in 1976. 2,22 The biosynthesis of this antibiotic was studied by the groups of Vleggaar 23 and, more recently, Tang and co-workers, 24 the latter of whom proposed a complete biosynthetic pathway. Specifically, as shown in Scheme 1, it was suggested that the EF-spirosystem is formed from polyketide 15 and a unit of geranyl pyrophosphate (16) as facilitated by VrtC, a polyketide prenyltransferase. 25 This reaction is followed by oxidative cyclization catalyzed by another Vrt enzyme (VrtK, a cytochrome P450-type enzyme) 26 to afford viridicatumtoxin A (2) through transient intermediates 19−21 (on the basis of computational studies), as shown in Scheme 1.
In 2008, Kim et al. 1 reported the isolation of viridicatumtoxin B in small quantities along with viridicatumtoxin A (2) from Penicillium sp. FR11 and, on the basis of NMR spectroscopic analysis, assigned the hydroxy-epoxide structure 1′ (Chart 1) to the former. These investigators observed potent activities for viridicatumtoxins A and B against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) (MIC = 0.25 and 0.5 μg/mL, respectively). Interestingly, a recent report suggested that the viridicatumtoxins exert their antibacterial properties through inhibition of UPP synthase, an important enzyme for bacterial peptidoglycan biosynthesis. 3,27 This stands in contrast to the mode of action of other tetracyclines (e.g., 4−10, Chart 2a), which inhibit bacterial protein synthesis by binding to the 30S subunit of the ribosome.
In view of the scarcity of viridicatumtoxin B, its interesting but suspect structural assignment (i.e., 1′, Chart 1), and its important biological activity, we initiated a program directed toward its total synthesis. Herein, we describe details of our investigations that led to important new knowledge and insights with regard to this intriguing bioactive molecule. 28 ■ RESULTS AND DISCUSSION First-Generation Approach: Development of an Anthrone Alkylation and Lewis Acid-Mediated Spirocyclization. The intrigue surrounding the originally assigned structure of viridicatumtoxin B (1′) is derived from its uncommon epoxy-hemiacetal structural motif, its lipophilic spirocyclic domain, and its high oxygenation, particularly on ring B. Although the first and rather curious structural feature may have an explanation in this instance originating from the surrounding functionalities of the molecule, from the synthetic point of view, it was reasoned that it could be resolved by Chart 2. Molecular Structures of (a) Bacterial Tetracyclines and Designed Analogues and (b) Fungal Tetracyclines targeting either structure 1′ or 1 (Chart 1) and allowing their potential thermodynamic equilibration to provide an answer. The spirocycle provided a synthetic challenge that we decided to face following the proposed biosynthesis, which inspired a Friedel−Crafts-type approach (see Scheme 1). The remaining major challenge of a synthesis of viridicatumtoxin B was initially relegated to a rather speculative singlet oxygen [4 + 2] cycloaddition to an appropriately substituted aromatic ring (B). Finally, the construction of the enol−amide structural motif characteristic of most tetracyclines was to be derived from an isoxazole ring through hydrogenolysis, a well-tested and reliable tactic introduced by Stork and Hagedorn. 18a These considerations led to our first retrosynthetic analysis of viridicatumtoxin B as depicted in Scheme 2. Thus, it was reasoned that 1″ or 1 could be derived from bis-epoxide structure 22 (R = selectively cleavable protecting group), which could be traced to endoperoxide 23 through a radical-based rearrangement and thence to aromatic system 24 via selective oxygen addition to the electron-rich B-ring. This scenario was inspired by a number of previous relevant studies, highlights of which are shown in Scheme 3. 29 Thus, endoperoxide 29 (generated from singlet oxygen and tetracene) was reported to undergo a thermally-induced rearrangement to bis-epoxide 31, presumably via diradical 30, and the latter was trapped with Nmethylmaleimide (32) to afford adduct 33 (Scheme 3a). 29e The regioselectivity of the latter reaction is of note in that it reflects different reactivities for the benzene and naphthalene systems. A similar type of fragmentation was induced within endoperoxide 34 by an iron(II) species, leading to bis-epoxide 38, as shown in Scheme 3b. 29k This rearrangement is presumed to proceed through the sequential one-electron transfers involving transient species 35−37. 30,31 Photochemicallyinduced homolytic rupture of endoperoxides is also possible as demonstrated in Scheme 3c. 29c Specifically, endoperoxide 40, generated from naphthalene derivative 39, formed bis-epoxide 42, presumably via diradical 41. Further elaboration of 42 led first to syn diol 43 and thence dihydroxy quinone 44, demonstrating the accessibility and versatility of the endoperoxide moiety. 32 The synthesis of allylic chloride fragment 27 began with geranic acid (45) and followed a modified sequence based on a literature process, 33 as shown in Scheme 4. Thus, acid-mediated cationic cyclization (H 3 PO 4 ) of 45 followed by methylation (MeI, K 2 CO 3 ) of the resulting acid produced methyl ester 46. Epoxidation of the latter with m-CPBA furnished a mixture of epoxide isomers which was treated with NaOMe to give the desired allylic alcohol methyl ester 47 (70% for four steps). Protection of the hydroxyl group of 47 (TBSCl, imid.) and subsequent reduction (DIBAL-H) then led to allylic alcohol 48 (91% for two steps). Attempted mesylation of 48 with MsCl/ Et 3 N led to partial formation of allylic chloride 27, presumably via the initially formed mesylate 49. It was then found that treatment of 48 with MsCl/Et 3 N followed by addition of LiCl produced the allylic chloride 27 in satisfactory yield (48%).
With allylic chloride 27 readily available, we next turned our attention to the construction of anthrone 28 (Scheme 5). Bromo-benzyl juglone 51 was prepared in 66% overall yield following a two-step literature procedure starting from juglone (50). 34 Known Brassard diene 53 was prepared in one step starting from methyl 3-methoxybut-2-enoate (52) via formation of the corresponding enolate and subsequent trapping with TMSCl (99%). 35 With these building blocks in hand, their fusion through a Diels−Alder reaction was performed by mixing the dienophile 51 with 3 equiv of diene 53 at −30°C and allowing the reaction mixture to reach ambient temperature. The TMS ether of the initially formed Diels−Alder adduct 54 was cleaved with silica gel, which caused collapse of the ketal and spontaneous elimination of HBr (see 55, Scheme 5) followed by tautomerization, ultimately producing anthraquinone 56 in 90% overall yield. Similar types of cascade Diels−Alder/elimination sequences have been performed previously. 36 Methylation of the free phenolic group of 56 (MeI, K 2 CO 3 ) followed by regioselective deoxygenation (SnCl 2 , HCl/AcOH) and concomitant debenzylation then furnished anthrone 28 (86% yield for two steps). 37 Previous reports have detailed the successful alkylation of the methylene position of anthrones with simple alkyl halides. 38 These studies also provided precedent for alkylation at the methylene position in preference to the free phenolic position(s), the latter being deactivated by hydrogen bonding with the adjacent carbonyl group. Nevertheless, the alkylation of an anthrone with a more complex electrophile was unprecedented and had not been utilized previously in the context of tetracycline synthesis. It was, therefore, pleasing to find that union of anthrone 28 with chloride 27 was successful when performed at 50°C, employing Na 2 CO 3 as the base in the presence of catalytic quantities of KI, providing the desired product 58 in 51% yield (ca. 1:1 dr). It is worth noting that the success of this reaction depended on degassing the solution and conducting the reaction in the absence of light 39 to prevent oxidative radical dimerization of the anthrone substrate. 40 Initial attempts to form the EF spirosystem were based on precedents that typically employed unprotected allylic alcohols 41 or allylic acetates 42  were complicated with side-products, including elimination or allylic substitution products (e.g., allylic methyl ether with HCl/MeOH). These observations led us to hypothesize that it might be possible to directly ionize the TBS ether of 58, thereby eliminating the need for the deprotection step. After a brief screen of acidic conditions, it was found that ZnI 2 indeed promoted the desired transformation in 34% yield. As shown in Scheme 5, portionwise treatment of allylic TBS ether 58 in refluxing CH 2 Cl 2 with ZnI 2 produced spirocyclic compound 25 as a single detectable diastereomer. This reaction presumably proceeds via ionization of the TBS ether to generate allylic cation 59a/b. Then, intramolecular Friedel−Crafts-type reaction with the more electron-rich arene (ring D) followed by loss of a proton produces the observed product 25. We did not isolate any product resulting from Friedel−Crafts reaction on the B-ring, although some elimination products were observed. While the yield of this transformation was, for the time, rather modest, it was sufficient to allow us to probe the following steps. The stereochemical configuration of 25 was assigned by comparison with subsequent intermediates whose structures were unambiguously deduced from X-ray crystallographic studies (see below). The observed diastereoselectivity of the spirocyclization (58 → 25) can be rationalized by invoking the transition state resulting from conformer 59a as having the lowest energy barrier, as shown in Scheme 5. The transition state resulting from alternative conformer 59b, required to afford the other (15-epi-25, undesired) diastereomer, suffers from unfavorable interactions between one of the methyl groups and the arene, as indicated in structure 59b. These interactions are apparently not present in the transition state leading to the observed product (59a → 25).
As shown in Scheme 6, we next needed to perform a phenolic oxidation to render the B-ring of the growing molecule electrophilic, and thereby susceptible, to nucleophilic attack from a negatively charged A-ring isoxazole (i.e., anion of 26, see Scheme 2). The prototypical sequence of events for achieving this objective would involve PIDA [PhI(OAc) 2 ]-or PIFA [PhI(TFA) 2 ]-mediated oxidation of the phenolic moiety of 25 in MeOH to give intermediate 60, which may undergo a second oxidation to yield the desired quinone monoketal 61. 43 In reality, however, 25 proved intransigent to these projected transformations, leaving quinone monoketal 61 and its relatives, 60 and 64, elusive under a variety of conditions tested. These included hypervalent iodine-, molecular oxygen-, and metalbased oxidative conditions (see Supporting Information for details). Instead, benzylic oxidation leading to quinomethide 62 was observed in several cases, as well as decomposition under different conditions. In a few cases, undesired products involving oxidation of the F-ring were also observed.
Eventually, it was found that spirocycle 25 could be tautomerized to its desmotropic form 63 under basic conditions (i.e., K 2 CO 3 , Scheme 6). 44 When the latter compound was treated with PIDA in CH 2 Cl 2 /H 2 O the desired quinone 64 could be isolated in only 4% yield, along with substantial quantities of benzylic oxidation product 62 (13%) and recovered starting material (45%). A number of different oxidants (i.e., Fremy's salt, O 2 /salcomine catalyst, PIFA) were examined in efforts to improve the efficiency of the transformation of 63 to quinone 64, but unfortunately to no avail.
Unable to access quinone 64 in sufficient quantities, it was decided to focus our attention on an approach that would directly bring in the A-ring from an earlier stage, which would circumvent the problematic B-ring phenolic oxidation barriers we faced. Despite its failure to break through these barriers, however, the initial approach provided us with useful information and methods for the construction of the anthrone and allylic halide substrates and established the required spirocyclization reaction.
Second-Generation Approach: Attempts To Install the C4a and C12a Hydroxyl Groups via Dearomatization Pathways. The second-generation retrosynthesis of substrate 24, required for our endoperoxide rearrangement approach (see Scheme 2), is shown in Scheme 7. Thus, to avoid the oxidation problems discussed above, the subtarget molecule 24 was dissected through the C-ring, which retrosynthetically defined three fragments: allylic chloride 27, known homophthalic anhydride 66, 45 and AB-enone 65. The latter could be further disassembled into known quinone monoketal 67 46 and Stork−Hagedorn isoxazole 26. 18a The synthesis of requisite AB-enone 65 commenced from the known Stork−Hagedorn isoxazole 26, which was prepared using a modification of the literature procedure (see Scheme 8). Thus, the process for the synthesis of 26 involved acylation of dimethyl malonate (68) (MgCl 2 , Et 3 N, AcCl, 96%), 47 methylation of the resulting enol (69) with Me 2 SO 4 (54%), cyclization of 70 with H 2 NOH to afford compound 71 (48%), and benzylation of the resulting hydroxy-isoxazole (BnBr, Ag 2 O, 67%). The best conditions we found for the benzylation reaction were those employing Ag 2 O which provided the O-Bn in preference to the N-Bn product in ratios of up to 11:1. Alternative conditions gave unacceptably low selectivity ratios (Cs 2 CO 3 /BnBr: O:N ca. 1.25:1; TfOH/BnTCA: O:N ca. 1:7.5) in agreement with Kornblum's rule for ambident nucleophiles. 48 A procedure using phenyldiazomethane as the electrophile as used by Stork and co-workers 18a was considered undesirable due to safety concerns. With isoxazole 26 readily available, we next attempted its conjugate addition to quinone monoketal 67, as shown in Scheme 8. Thus, deprotonation of the methyl group of 26 with LiHMDS, followed by addition of quinone monoketal 67 to the resulting anion (26′), however, led to 1,2-addition product 72, rather than the desired 1,4product.
Faced with this predicament, we decided to install an electron-withdrawing group at the isoxazole methyl group, which we hoped would make the resulting nucleophile softer due to its vinylogous diketone nature. 18a Toward that end, and as shown in Scheme 9, treatment of isoxazole 26 with LiHMDS followed by quenching with methyl chloroformate provided 73 (96% yield). Michael addition of the latter to quinone monoketal 67 was then achieved under basic conditions (NaOMe) to give adduct 74 in 71% yield as an inconsequential ca. 3:1 mixture of diastereomers. Dieckmann condensation within 74 induced by NaH then led to tricycle 75 as a single diastereomer ( 3 J 4,4a = 11.2 Hz), presumably through epimerization at C4 during the reaction. Demethylation and subsequent decarboxylation of 75 was then carried out with Me 3 SnOH 49 at elevated temperatures, providing 76 in 39% yield for the two steps. Of note here is the observation that the use of LiOH proved unsuccessful in promoting this transformation. Subsequent oxidation of the latter substrate (i.e., 76) by way of selenide formation/oxidation/syn-elimination furnished compound 79 (54% for the two steps), through intermediate 77 and transient species 78. Finally, benzylation of the free phenolic moiety within 79 (BnBr, K 2 CO 3 , 94%) afforded the desired AB-ring system fragment 65 (mp = 103°C, Et 2 O). The structure of this key intermediate was unambiguously confirmed by X-ray crystallographic analysis (see ORTEP representation, Figure 1).
While the above route provided sufficient quantities of fragment 65 for preliminary studies, scaling up the synthetic sequence, especially the ester saponification with Me 3 SnOH, proved inconvenient. To circumvent this problem, we elected to change the C4-ester group to a more labile one. Alternative moieties considered were the ethoxyethyl ester, originally employed by Stork, 18a and the cyclic anhydride 80 (see Scheme 10), which was envisioned to undergo a one-pot Michael− Dieckmann/decarboxylation cascade sequence. Eventually, however, we selected a Teoc ester due to its anticipated orthogonality to the rest of the functionalities within the molecule. Thus, and as shown in Scheme 10, installation of the Teoc ester at C4 of isoxazole 26 was accomplished using a similar protocol as for the methyl ester (LiHMDS; then TeocCl, 88%) to afford derivative 81. Michael reaction of the latter with 67 (t-BuOK) then gave adduct 82 (71% yield, ca. 2.4:1 dr, inconsequential). At this point, it was gratifying to observe that the Teoc group could be removed in a straightforward fashion using TBAF at 25°C to furnish decarboxylated product 83 (85% yield). Dieckmann cyclization within 83, again induced by NaH, provided intermediate 76 (80% yield) which intersected the previously described route to 65 (see Scheme 9).
With AB-enone 65 in hand, our attention turned toward the synthesis of the desired ABCD-ring fragment 88 (Scheme 12) through union of the former with a suitable CD-ring precursor. Due to the observed 1,2-addition of isoxazole anion 26′ to quinone monoketal 67 (see Scheme 8), we anticipated the need for a soft CD-ring nucleophile. Toward this end, we selected the known homophthalic anhydride 66 [readily prepared in four steps from 4-chlororesorcinol (84) through intermediate 85 as shown in Scheme 11]. 45 Anhydride 66 has traditionally been used (after deprotonation with a strong base, typically NaH or LDA) in Tamura-type Diels−Alder reactions with quinones. 50 However, we found that substrate 66 could be united with AB-enone 65 using DBU as the base at 60°C to afford pentacyclic system 88 (82% yield, see Scheme 12). A 1 H NMR-tube experiment to monitor the reaction between 65 and 66 suggested that it proceeds via a distinct stepwise mechanism (intermediates 86 and 87 were tentatively observed; see selected peaks, Figure 2), as opposed to a concerted [4 + 2] cycloaddition followed by decarboxylation. Additionally, this experiment indicated that the reaction required approximately 20 h for completion. Elimination of methanol from cyclization product 88, induced by exposure to TFA in CH 2 Cl 2 , then led to elaborated anthrone 89 in quantitative yield. Attempts to alkylate anthrone 89, however, with chloride 27 (Scheme 12) under the conditions employed for the alkylation of the simpler anthrone 28 (Scheme 5) were thwarted by the insolubility of 89 in acetone and the low reactivity of 27, necessitating a search for a new protocol to achieve the desired union of the two partners. Thus, with further experimentation, it was found that upon using the corresponding allylic bromide 91 (prepared from allylic alcohol 48 by treatment with MsCl, Et 3 N, and LiBr in quantitative yield, Scheme 12) and changing the solvent from acetone to DMF, the required alkylation proceeded smoothly at ambient temperature and within 1 h to provide the desired coupling product 90 in 78% yield.
With intermediate 90 available, and in an effort to prepare subtarget 24 (Scheme 12), we then proceeded to apply our original spirocyclization conditions (i.e., ZnI 2 , CH 2 Cl 2 , reflux, see Scheme 5), but unfortunately encountered side-products, presumed to arise from elimination, 51 demethylation, 52 and/or benzylic oxidation reactions. We reasoned that such side reactions could be suppressed by using a nonmobile counterion on the activating agent, a hypothesis that prompted us to test BF 3 ·OEt 2 , a catalyst that held promise to also induce the pending spirocyclization at lower temperatures. Indeed, exposure of 90 to substoichiometric amounts of this Lewis acid at 0°C led to the formation of the desired heptacycle 24 in 55% yield with minimum amounts of side-products. The configuration of 24 was confirmed as shown in Scheme 12 by NOESY experiments.
Scheme 13 depicts our attempts to realize the singlet oxygen cascade sequence from 93 (derived from 24 via benzoate derivative 92) to 97 through postulated intermediates 94−96. Thus, heptacyclic phenol 24 was converted to its benzoate 92 (BzCl, Et 3 N, 79% yield) and thence to hexacyclic system 93 through hydrogenolysis (Pd black, H 2 , quantitative yield) of the N−O bond and the two benzyl ether moieties. Our reasoning for the protection of the phenolic group of 24 was based on the assumption that this change would promote the aromatization of ring C within 92 by removing the possibility for a H-bond to the C-ring carbonyl group. This assumption, however, proved erroneous, as both 92 and 93 were found to exist exclusively in their C-ring keto forms as opposed to their enol (phenolic) forms. In retrospect, this outcome is not surprising in light of previous reports, which indicated that conjugation of a naphthalene ring (as in 92 and 93) with a ketone provides thermodynamic stability greater than that of its phenolic counterpart (as in the phenolic tautomers of 92 and 93). 53 Anticipating the singlet oxygen-initiated cascade sequence of reactions shown in Scheme 13 (93 → 94 → 95 → 96 → 97) based on precedented chemistry 29 (see Scheme 3), we proceeded to react substrate 93 with singlet oxygen. This transformation, however, could not be realized. Thus, bubbling of O 2 through a solution of substrate 93 in CH 2 Cl 2 in the presence of catalytic quantities of TPP and with sun lamp irradiation at −78°C rapidly produced a product that proved, upon warming, too labile for characterization. Prolonged irradiation at −78°C produced no detectable amounts of bisepoxide 97. We then attempted to tautomerize the C-ring of 93 to its corresponding phenolic form under basic conditions with the hope that this maneuver (see above, Scheme 6) might lead to a favorable result in the singlet oxygen reaction. However, the previous conditions used for the corresponding keto− phenol tautomerization (25 → 63, K 2 CO 3 , MeOH, Scheme 6) failed to yield the desired product. Other efforts to achieve the desired outcome included attempts to form and rearrange the endoperoxide using different sensitizers on substrates with and without the isoxazole ring, the latter scenario being inspired by Myers's hypothesis that the isoxazole moiety may act as a selfsensitizer. 20a These experiments failed to produce any of the proposed intermediates in Scheme 13 (i.e., 94−97).
On the basis of these findings, we were forced to explore alternative phenolic oxidation protocols in an effort to install oxidation at C12a (Scheme 14). Attempted phenolic oxidations of substrate 24, or its corresponding hydrogenolyzed counterpart 98, were unsuccessful. A number of other conditions, including hypervalent iodine reagents, Fremy's salt, Pb(OAc) 4 , and various metals with molecular oxygen, were also unsuccessful. Typically either no reaction was observed or the substrate decomposed, especially for the more electron-rich substrate 98. Furthermore, phenol 101 was synthesized as a possible substrate for C12a hydroxylation through a sequence involving PIFA oxidation to the quinone, benzoyl ester formation (BzCl, 65% for two steps), and regioselective debenzylation (MgBr 2 ·OEt 2 , 75%). Phenolic oxidation of this substrate (i.e., 101), however, also failed to produce the desired C12a oxidation product 102. The challenges faced here with respect to installation of the C12a hydroxyl group are reminiscent of previous synthetic efforts toward tetracyclines, including those of Woodward, 13 Muxfeldt, 15 Stork, 18 and, in particular, Barton. 54 The failure to install the obligatory hydroxyl groups at C4a and C12a through dearomatization pathways refocused our attention on alternative tactics, the choice of which was based on the intelligence gathered thus far. This information pointed away from an aromatic A-ring as a precursor to the desired oxygenation.
Third-Generation Approach: Stepwise Strategies To Install the C4a and C12a Hydroxyl Groups. Our first attempts to install the coveted hydroxyl groups onto the substrate via enol or enolate chemistry focused on the rather simple AB-ring fragment 76 (Scheme 15). As shown in this scheme, treatment of 76 with DMDO gave a mixture of C12ahydroxylated compounds 103 and 12a-epi-103 (ca. 3:5 ratio, 79% yield combined, chromatographically separated, inconsequential) whose configurations were established through analysis of their NOESY correlations and 2,3 J coupling constants (see structure 12a-epi-103). We envisioned that the extrusion of methanol from 103 might result in the formation of methyl enol ether 105, whose selective epoxidation would furnish 106. Such a strategy would be conceptually similar to that of Myers, which features a fully functionalized AB-ring enone system. However, the elimination of methanol (103 → 104 → 105, Scheme 15) turned out to be surprisingly problematic. A variety of Lewis-or Brønsted-acidic conditions failed to promote this transformation, leading instead to various decomposition side-products, including lactone ring expansion products involving the C12a hydroxyl group. We reasoned that these failures may be due to mismatched electronics which did not allow for the formation of the oxonium moiety adjacent to the enone system. We then speculated that the presence of an aromatic ring adjacent to the oxonium system (see structure 104, Scheme 15) might facilitate the formation of such a species.
With these postulates in mind, a new strategy was devised based on the retrosynthetic analysis shown in Scheme 16, which most notably circumvents an aromatic A-ring and postpones the C4a/C12a oxygenations to the later stages of the projected synthesis. Thus, viridicatumtoxin B structure 1″ was disconnected through the A-ring with a Michael−Dieckmann reaction similar to that which had successfully been deployed in the second-generation strategy. This analysis unveiled isoxazole fragment 81 and BCDEF pentacycle 61 as potential key building blocks. The latter was envisioned to be derived from fragments 91, 66, and 67 through a pathway similar to that of the second-generation approach involving homophthalic anhydride/quinone monoketal cyclization, anthrone alkylation, and Lewis acid-mediated spirocyclization (see Scheme 12).
As shown in Scheme 17, a similar set of reaction conditions as those used to prepare 24 (see Scheme 12) were employed to synthesize spirocyclic compound 60. Thus, DBU-promoted Michael−Dieckmann/decarboxylation cascade (107, 50% yield) and CSA-catalyzed aromatization (quant.) gave anthrone 108. Anthrone alkylation with the allylic bromide 91 then gave the alkylated compound 109 (75%, ca. 1:1 dr, inconsequential). The steric congestion around the newly generated C−C bond of the latter compound manifested itself in some unusual NMR spectroscopic features. Thus, all three methyl groups of the Fring in 109 showed very broad signals, which suggested hindered rotation around this region of the molecule at 298 K, thereby forcing the methyl groups to experience a number of different magnetic environments. Supporting this notion was the fact that when the 1 H NMR spectrum of 109 was acquired at 338 K in DMSO-d 6 , the methyl signals sharpened considerably. Spirocyclization of 109 proceeded in the presence of BF 3 ·OEt 2 as before, producing the desired pentacyclic compound 60 in 82% yield. An X-ray crystallographic analysis of 60 [mp = 114−116°C (EtOAc:CHCl 3 1:1), see ORTEP, Figure 3] unambiguously proved its spectroscopically derived structural assignment. 55 With key intermediate 60 now accessible, we turned next to its phenolic oxidation. Although initially concerned, due to the previous recalcitrance of substrate 25 (see Scheme 6) to undergo phenolic oxidation, these trepidations proved to be unfounded.
Thus, in an initial experiment, treatment of spirocycle 60 with PIDA (3.0 equiv) led to the formation of desired quinomethide−quinone monoketal 111 (54% yield) and sideproduct 112 (22%, Scheme 18). The stereochemical configuration of the latter species was initially assigned on the basis of a NOESY experiment and was later unambiguously confirmed through X-ray crystallographic analysis [mp = 201−203°C (EtOAc:CH 2 Cl 2 1:1), see ORTEP representation, Figure 4].  The formation of both 111 and 112 in the presence of excess PIDA in MeOH at 25°C is apparently a consequence of the competing pathways shown in Scheme 18. Thus, initial phenolic oxidation of 60 affords 61, which suffers elimination of MeOH induced by the in situ-generated AcOH leading to phenol p-quinomethide 110 (isolated and characterized), whose phenolic oxidation furnishes 111. Intermediate 61, however, may also undergo phenolic oxidation, furnishing 112. The facility by which substrate 60 undergoes phenolic oxidation as compared to its simpler counterpart 25 mentioned above (see Scheme 6) is attributed to the extra methoxy group residing on ring B of the former. Attempts to convert side-product 112 to the desired compound 111 under acidic, basic, or thermal conditions failed.
Efforts to optimize the conversion of 60 to 111 led to the preferred three-step sequence (via 61 and 110) shown in Scheme 18. Thus, employment of limited quantities of PIDA (1.2 equiv), MeOH:CH 2 Cl 2 (1:1) as solvent, and lower temperature (0 → 25°C), generated intermediate 61 in high yield. The crude product in CH 2 Cl 2 was then treated with catalytic quantities of CSA at 0°C to provide p-quinomethide 110, which was subjected to further phenolic oxidation with PIDA in MeOH to afford 111 in 78% overall yield for the three steps. It should be mentioned here that substrate 61 proved intransigent to the desired pending reactions (i.e., Michael− Dieckmann cascade), leading instead to MeOH-elimination product 110. Access to substrate 111 opened the way forward, despite the need for reduction of the p-quinomethide to the desired phenolic structural motif at a later stage. The feasibility of such a transformation was briefly explored at this stage, leading to the finding that it could be achieved with NaCNBH 3 (Scheme 18, 111 → 61, 29% yield, unoptimized).
The next task was the union of pentacyclic enone 111 with isoxazole 81 through the Michael−Dieckmann sequence of reactions as indicated in Scheme 19. On the basis of our success with this sequence on the simpler systems discussed above (i.e., Schemes 9 and 10), we were rather optimistic for favorable results in this instance as well. However, initial experimentation with methyl ester 81 as the isoxazole equivalent proved  otherwise. Thus, although the first step (Michael reaction, Scheme 19) of the anticipated fusion of 111 with 81 proceeded well, leading to Michael adduct 113 (t-BuOK cat., 74% yield, mixture of four diastereoisomers), conditions for the subsequent step (Dieckmann reaction) proved elusive despite considerable experimentation (a variety of basic conditions typically led to recovery of unreacted 113 or decomposition). Removal of the Teoc group from 113 led to 115 and its C15epimer (15-epi-115, ca. 2:1 dr) 56 (TBAF, 60% yield), which, however, also failed to undergo the coveted Dieckmann cyclization (to 116, Scheme 19). Reduction of the quinomethide moiety of 115/15-epi-115 furnished 117/15-epi-117 (NaCNBH 3 , 82% yield, ca. 2:1 dr). The dibenzyl ether (119/ 15-epi-119) of the latter was also prepared (NaH, BnBr, TBAI cat., 85% yield, ca. 2:1 dr). Neither 117/15-epi-117 nor 119/ 15-epi-119 entered the desired Dieckmann reaction to afford the expected heptacyclic systems 118/15-epi-118 or 120/15epi-120, respectively. Similar problems were reported by Stork and Kahne. 57 Faced with these difficulties and inspired by the work of White 58 and Myers, 20d we opted to employ the phenyl ester counterpart of isoxazole 81 (see 123, Scheme 20a). Isoxazole phenyl ester 123 was prepared from methyl ester 26 via 121 and 122 through saponification (NaOH, 99% yield), esterification with PhOH under Mitsunobu conditions (PPh 3 , DIAD, 78% yield), 59 and Teoc attachment (LiHMDS, TeocCl, 86% yield) as shown in Scheme 20a. It is of note that several standard ester-forming reactions (e.g., mixed anhydride, acid chloride, DCC) failed to bring about the desired esterification between 121 and PhOH. Gratifyingly, quinone monoketal 111 reacted smoothly with phenyl ester isoxazole 123 in the presence of a slight excess of t-BuOK to give directly the desired heptacyclic compound 114 as a ca. 1:1 mixture with its C-15 diastereoisomer (15-epi-114) in 94% yield (Scheme 20b). 60 The stereochemical relationship of H4 and H4a within 114 was evident from the observed coupling constant 3 J 4,4a = 9.9 Hz, indicative of the diaxial disposition of these protons. The impressive rapidity (<15 min at 25°C) of the Michael− Dieckmann cascade to afford the heptacyclic product (111 → 114 + 15-epi-114) is a testament to the activating power of the phenyl ester moiety in such circumstances.
We initially explored the C12a hydroxylation of substrate 114, with the Teoc group still intact, as shown in Scheme 21.
The difficulties of removing the Teoc group in the presence of the C12a hydroxyl group led us to explore the alternative sequence in which the Teoc moiety was removed prior to the hydroxylation step. To this end, and as shown in 116, 16% combined yield) and unreacted starting material (33%). Presumably, this failure was caused by deprotonation of the diketone moiety adjacent to the isoxazole (see 129) that rendered the desired loss of TMSF, ethylene, and carbon dioxide less facile (see 129 → 130). Faced with this predicament, we undertook a thorough investigation of conditions to achieve the desired Teoc group removal from 114 (+ 15-epi-114), including ZnCl 2 , ZnF 2 , MgBr 2 , NH 4 F, and SiF 4 , 61,62 but unfortunately to no avail. Disappointingly, the use of HF·Et 3 N led to the desired product 116 (+ 15-epi-116) in 28% combined yield, albeit with substantial amounts of sideproduct 131 (30% yield), presumably formed through an airoxidation process. 63 Indeed, it was not until we combined Furstner's TBAF−NH 4 F conditions 64 with solvent degassing (to prevent air oxidation of the A-ring) that we reached a solution to this problem, obtaining a pleasing 98% yield of the coveted deprotected product 116 (+15-epi-116) (Scheme 22).
Having solved the Teoc removal problem, we turned our attention to the C12a hydroxylation task using 116/15-epi-116 as a substrate. Our initial use of DMDO in acetone at low temperature (i.e., −78°C) proved irreproducible, gave low conversion to product due to substrate insolubility, and suffered from TLC monitoring problems. To improve the reaction, we resorted to the use of Ni(acac) 2 , an additive known to facilitate DMDO oxidations through coordination. 65 66 and magnesium monoperoxyphthalate (MMPP) proved unsuccessful in providing the desired C12a-hydroxylated compound (i.e., 132 +15-epi-132).
With the C12a hydroxylation problem solved, the challenge of installing the C4a hydroxyl group became the next task, a mission that was to prove even more intransigent than the one before it. Our initial attempts to achieve this goal are shown in Scheme 23. Thus, reduction of the quinomethide moiety of 132/15-epi-132 with NaCNBH 3 led to the now chromatographically separable derivatives 133 and 15-epi-133. Gratifyingly, one of the two isomers (more polar) crystallized readily from EtOAc [mp = 213−215°C (decomp)] and yielded to Xray crystallographic analysis, establishing its stereochemical configuration as 15-epi-133 (see ORTEP representation, Figure  5), and as a consequence, that of its less polar epimer as the desired diastereomer 133. 55 Several attempts to prepare methylenol ether 134 as a potential precursor of the desired 4a-hydroxylated compound 136 through selective epoxidation (to afford 135) and thence hydrolysis, or through 137 via hydrolysis, enolization, and oxidation, failed. Efforts to generate 134 from 133 employing a variety of conditions, including Lewis acids or protic acids, or under anhydrous conditions (see Supporting Information), however, did not give the desired product but led instead to ketone 137. Similarly, various conditions to carry out the desired enol ether formation at the quinomethide stage (compound 132/15-epi-132) also failed. In addition, a number of transketalization reactions with dimethyl ketal 133 directed at forming an oxa-seleno mixed ketal 67 (intended to be used as a precursor for a selenoxide/syn elimination sequence to produce the desired methylenol ether 134) ended without success. Attempts to achieve C4a hydroxylation of ketone 137 through enolization followed by oxidation also failed, typically resulting in aromatization to afford 138 (Scheme 23) through β-elimination/tautomerization (see Supporting Information for more details).
We then explored the possibility of achieving C4a hydroxylation using dimethyl ketal 133 (single diastereomer) to generate olefinic compound 141, whose directed dihydroxylation 68 was expected to yield 142 (see Scheme 24). To this end, exposure of 133 to NaCNBH 3 in AcOH produced chromatographically separable methyl ethers 140 (40%) and 5epi-140 (32%), presumably through reactive oxonium species 139. 69 The configuration of 140 (and by deduction, of 5-epi-140) was assigned on the basis of 2D NMR (NOESY) spectroscopic analysis and the observed 3 J 4a,5 = 8.7 Hz coupling constant (see structure 140, bottom of Scheme 24). Numerous attempts (e.g., Lewis and protic acids) to generate olefin 141 from 140 or 5-epi-140 through elimination of MeOH, however, failed. In several cases, the bis-elimination/tautomerization product 143 was isolated instead. Attempted solvolysis of isomer 5-epi-140 by heating in trifluoroethanol at 70°C as a means to reach olefin 141 led, surprisingly, to trifluoroethyl ester 144 (70% yield, Scheme 24), presumably through a retro-Dieckmann ring opening reaction. 70 Unable to reach the Δ 4a,5 -compound 141 (Scheme 24), we turned to the Δ 4,4a -compound 145 as an alternative substrate for the desired C4a hydroxylation. It was envisioned that 145 could be reached from 133 through an oxidative process and then converted to the desired hydroxylated product 147 via selective formation and regioselective reductive opening of epoxide 146, as outlined in Scheme 25. As shown in Scheme 26, execution of this plan began with formation of presumed trianion 148 (KHMDS, −78°C) followed by quenching with PhSeCl (−78 → −40°C) to afford phenyl selenide 149 as a single isomer (46% yield + 26% recovered starting material). The configuration of the phenylseleno group within 149 was tentatively assigned on steric grounds (i.e., concavity of the cisfused AB-ring system). Treatment of selenide 149 with DMDO at −78°C produced a complex mixture from which sevenmembered lactone 152 could be isolated (46% yield), presumably formed through the indicated pathways involving selenoxide 150, olefin 145, and either acylketene 151 (pathway A) 71,72 or hydroxy-epoxide 153 (pathway B) (see Scheme 26). Other oxidizing agents (e.g., H 2 O 2 , m-CPBA) led to similar results. In an attempt to shut down the hypothesized hydroxyepoxide pathway, we prepared TMS ether 154 from 149 (TMSOTf, Et 3 N, 62% yield, Scheme 26) and employed it as a substrate in the DMDO oxidation, only to observe the same ring expansion product 152, albeit in only 7% yield (with concomitant loss of the TMS residue). This outcome seems to tentatively support pathway A, rather than B, for the formation of 152 from 154 (and 149), although due to the lability of the TMS group the possibility of both pathways operating concurrently cannot be excluded.
Faced with these obstacles toward Δ 4,4a -substrates 145 and 157, we decided to return to Δ 4a,5 -substrate 141 which we had previously considered but later abandoned (see Scheme 24). Inspired by a report from the Tatsuta group, 75 we reasoned that a 1,3-directed reduction of the C5-carbonyl moiety at the 137 stage could give us access to the C5-hydroxy compound 162, whose dehydration would lead to the targeted substrate 141 that could serve as a precursor to the desired trihydroxylated intermediate 142 (Scheme 29). However, when 137 was treated with NaBH(OAc) 3 , we instead isolated the 1,2-directed reduction product 164 (35% yield, Scheme 29), along with other unidentifiable products. After further unsuccessful attempts to secure the targeted C5-hydroxy derivative of our heptacyclic compound, we opted to pursue the newly emerged 1,2-directed reduction product 164 as a possible substrate for advancement to viridicatumtoxin B. The rationale behind this choice included the expectation that this intermediate and its derivatives could be resistant to elimination and aromatization side reactions involving rings A and B as previously observed (see Schemes 23 and 24). Brief experimentation led to an improved protocol for the selective reduction of triketone 137 [NaBH(OAc) 3 , EtOAc:acetone] that delivered alcohol 164 in 53% yield (Scheme 30). The newly generated hydroxy group within the latter compound was protected as a TBS ether through the use of excess TBSOTf (most likely needed due to steric difficulties caused by the concavity of the AB-ring system) to afford compound 165 (64% yield). Conversion of the latter compound to its trianion (166) with KHMDS followed by quenching with Davis oxaziridine led to the coveted C4 hydroxylated product 167 in 29% yield (47% based on 39% recovered starting material). Alternative bases (LiHMDS, NaHMDS, or LDA) or equivalents of base did not improve the outcome of this reaction. The configuration of the newly generated C4a hydroxyl moiety within 167 was deduced from NOESY correlations as shown in Scheme 30 (bottom).
In an effort to improve the C4a hydroxylation reaction, we targeted bis-TBS ether 168 (Scheme 31) as a substrate, counting on its dianion to undergo the desired transformation more efficiently. Bis-silylation of 164 with excess TBSOTf required the use of Hunig's base and led to 168 in 57% yield. Interestingly, however, treatment of 168 with KHMDS at −78°C led rapidly to aromatized product 172 (43% yield), which, in addition to elimination of the OTBS group from its A-ring, had also suffered removal of the TBS group from the phenolic oxygen. These observations suggest that the presumed initially formed dianion 169 is too reactive entering the proposed cascade sequence shown in Scheme 31. Thus, attack of the tertiary alkoxide within 169 on the adjacent carbonyl moiety generates a new alkoxide anion that abstracts the TBS group from the adjacent phenolic position to afford epoxide species 170. Prompted by the adjacent enolate anion, the epoxide within the latter species undergoes facile β-elimination/opening with concomitant loss of the B-ring OTBS group to afford diketone dianion 171. The generation of the newly established anion at C4 within 171 is facilitated by the isoxazole ring on one side and the quinone moiety on the other. Species 171 then suffers OTBS elimination/ring A aromatization, leading to observed product 172 upon protonation. Taken together, these extensive investigations on the C4a hydroxylation defined the required structural features for the substrate as (a) protected hydroxyl group at C1 (ring A), (b) free hydroxyl group at C12a (rings A/B), and (c) free phenolic moiety at C11 (ring C). Straying away from any one of these key structural motifs led to either no conversion or rearranged products of undesired structures.
At this point, we decided to move forward and test the remaining steps of the devised synthetic strategy toward viridicatumtoxin B that required reinstallment of the carbonyl group at C1, selective removal of the methyl group from the C10 methyl ether, and rupture of the isoxazole moiety to provide the required enol amide functionality at the terminus of the molecule. To this end and as shown in Scheme 32, silyl ether 167 was desilylated (HF·py, 66% yield) to afford C1 hydroxy compound 173 which was assumed to exist in equilibrium with its hemiacetal form 173′ based on its broad 1 H and 13 C NMR spectroscopic features. Oxidation of this mixture with DMP resulted in the formation of ketone 136 (32% yield, unoptimized) through reaction of 173, which apparently drives the equilibrium in the right direction for funneling both isomers (173 and 173′) toward the desired product (136). The obligatory removal of the methyl group from the C10 phenolic group, however, proved difficult despite the inspiring precedent for such deprotections in the tetracycline series. 19,20b Thus, attempts to achieve this goal with BCl 3 , BBr 3 , AlCl 3 /EtSNa, and 9-I-BBN 76 failed, leading to either decomposition and/or cleavage of the benzyl ether as indicated by LCMS detection of hydroxy-isoxazole 174 (but not 175).
In contrast to this disappointing result, the rupture of the isoxazole ring planned as the ultimate step of the synthesis proved feasible with substrate 136 under hydrogenation conditions in the presence of Pd black, furnishing 10-Omethylviridicatumtoxin B (176, 57% yield) as shown in Scheme 33. Interestingly, the spectroscopic data of this compound confirmed its hydroxy-keto form 176 rather than the originally assigned 1 (to viridicatumtoxin B) hydroxy-epoxide structure (i.e., 1′, Chart 1). The path-pointing results and intelligence discussed above served as the basis for the final strategy and drive toward viridicatumtoxin B.
Total Synthesis and Structural Revision of Viridicatumtoxin B: Final Drive. Scheme 34 presents the final retrosynthetic analysis and successful strategy that led to the total synthesis of viridicatumtoxin B. Thus, cyclic anhydride 177, quinone monoketal 67, allylic bromide 91, and isoxazole 123 were confidently defined as the required building blocks for the intended route (based on the studies described above). The high modularity of the strategy (i.e., four building blocks of approximately equal sizes) and the accessibility of the starting materials appeared attractive for further development and designed-analogue construction. The adoption of the benzyl ether protecting groups at both the anhydride (177) and isoxazole (123) fragments offered the advantage of their concurrent removal as the last step in the synthesis. The overall plan called for the union of the anhydride (i.e., 177) and quinone monoketal (i.e., 67) fragments through (1) a Michael−Dieckmann/decarboxylation cascade to cast the C ring, (2) an S N 2 alkylation to attach the F ring, (3) a Lewis acid-catalyzed spirocyclization to cast the E ring, and (4) a Michael−Dieckmann sequence to fuse the A-ring as outlined in Scheme 34.
Access to building blocks 67, 91, and 123 has already been discussed above, leaving only the preparation of the benzylprotected anhydride fragment 177 to be described here. Its synthesis and union with the other fragments (67, 91, and 123) are summarized in Scheme 35. Thus, regioselective demethylation of the known diethyl ester 85 with BBr 3 followed by benzylation (BnBr, Ag 2 O) led to benzyl ether 178 in 66% overall yield. Saponification of the ester moieties of the latter (aq NaOH) and heating of the resulting dicarboxylic acid (179) with Ac 2 O as previously reported 45 furnished desired anhydride 177 in 90% overall yield.
Anhydride 177 was reacted with quinone monoketal 67 through the action of NaH (Michael−Dieckmann sequence) followed by treatment of the coupling product with DBU (decarboxylation) to afford tricycle 180 in 54% overall yield. Elimination of MeOH from the latter induced by CSA gave the corresponding anthrone (99% yield), which was alkylated with allylic bromide fragment 91 in the presence of Na 2 CO 3 to furnish intermediate 181 (77% yield). Exposure of 181 to catalytic amounts of BF 3 ·OEt 2 afforded spirocycle 182 in 73% yield. The latter was then treated with PIDA in MeOH:CH 2 Cl 2 to give the initially formed quinone monoketal 183 and thence with CSA to afford the phenol p-quinomethide 184 (85% yield overall). Phenolic oxidation of the latter with PIDA in MeOH:CH 2 Cl 2 then delivered 185 in 90% yield, as observed previously with the corresponding C10-methyl series of substrates (see Scheme 18). Coupling of 185 with isoxazole phenyl ester fragment 123 was effected with t-BuOK (Michael reaction/Dieckmann condensation) furnishing heptacyclic compound 186 together with its unseparable C15 epimer (15-epi-186, ca. 2:1 dr, 91% combined yield). Removal of the now-superfluous Teoc group was accomplished through application of the developed desilylation/decarboxylation procedure (TBAF/NH 4 F) to furnish advanced intermediate 187 together with its unseparable isomer 15-epi-187 (ca. 2:1 dr) in 86% combined yield.
The next step in the synthesis entailed hydroxylation of the molecule at C12a (see Scheme 36), a process that was plagued with increased insolubility issues of the benzyl-protected substrate 187/15-epi-187 as compared to the methyl-protected version employed in the earlier generation route. This necessitated the use of DMDO in the presence of Ni(acac) 2 in CH 2 Cl 2 (instead of acetone); the efficiency of the reaction remained similar as the previous result after recycling of recovered starting material, yielding C12a-hydroxylated compound 188 together with its 15-epimer (15-epi-188, 35% yield, 50% yield after one recycle, ca. 2:1 dr, Scheme 36). Employing a solution of DMDO in CH 2 Cl 2 77 did not result in a noticeable improvement of conversion or yield, which might be attributed to the decreased stability of DMDO in that solvent. Exposure of the quinomethide mixture (188 + 15-epi-188) to NaCNBH 3 gave chromatographically separable reduced products 189 (39% yield) and 15-epi-189 (19% yield) whose configurations were assigned by comparisons of their NMR spectroscopic data to those of their corresponding C10-OMe counterparts (as discussed above, the structures of the latter were unambiguously assigned through X-ray crystallographic analysis, see Scheme 23 and Figure 5). Isomer 189 was then advanced to TBS ether 192 as shown in Scheme 36. Thus, treatment of 189 with HCl in aqueous THF led quantitatively to triketone 190, whose reduction with NaBH(OAc) 3 furnished C1-hydroxy compound 191 in 47% yield. The latter was then silylated with TBSOTf in the presence of 2,6-lutidine to afford 192 (61% yield).
The final stretch of the synthesis of viridicatumtoxin B is depicted in Scheme 37. The challenging C4a hydroxylation of precursor 192 proceeded in the presence of KHMDS (added first at −78°C to form the presumed trianion of 192) and Davis oxaziridine (added thence at −78°C) to afford hydroxylated product 193 (20% yield + 45% recovered starting material). Desilylation of 193 with HF·py furnished polyhydroxy compound 194, whose equilibrium with its lactol form (194′) was evident from the 1 H NMR spectra (CDCl 3 , 500 MHz) at ambient temperature (broad signals) and −40°C (two sets of sharp signals, ca. 2:1 ratio). (This was the same phenomenon we encountered with their 10-methoxy counterparts 173/173′, Scheme 32, as discussed above). Oxidation of the so-obtained mixture (194 + 194′) with DMP then gave triketone 195 in 66% yield, with the lactol form 194′ being funneled into the oxidation pathway by the equilibrium. Pleasantly, hydrogenation of the latter compound (Pd black, H 2 ) produced synthetic viridicatumtoxin B (1) in 98% yield through cleavage of the two benzyl ethers, rupture of the isoxazole N−O bond, and tautomerization of the resulting hydroxy-imine to the desired primary amide.
The physical properties of synthetic viridicatumtoxin B were consistent with those of the authentic natural product and our proposed structure (1). The 1 H NMR data of synthetic viridicatumtoxin B (1) were in good agreement with those reported in the literature. 1 However, we observed a 13 C NMR signal at δ = 194.1 ppm for C5, whereas the reported chemical shift for the proposed C5 epoxy-hemiacetal in the originally assigned structure was δ = 116.4 ppm. Therefore, a detailed reexamination of the authentic carbon NMR spectrum (obtained from Professor W. G. Kim) of natural viridicatumtoxin B was conducted. Indeed, the signal near δ = 194 ppm was observable in the authentic spectrum of the natural product. We assume that, due in part to the minute amounts of this complex natural product isolated, an erroneous interpretation of the HMBC spectrum seemingly supported the original structural assignment (see the Supporting Information for more details and copies of the authentic spectra). We therefore revised the originally proposed structure of viridicatumtoxin B (1′) 1 to that shown in Scheme 37 (i.e., 1). Furthermore, our synthetic material crystallized from CH 2 Cl 2 /EtOH in suitable form for Xray crystallographic analysis [mp = 245−247°C (decomp)] and proved unambiguously its structure as shown in 1 (see ORTEP representation, Figure 6). 55 Biological Evaluation of Synthetic (±)-Viridicatumtoxin B and Analogues. Employing the developed synthetic technologies in this research program, we were able to access not only (±)-viridicatumtoxin B [(±)-1] but also a number of analogues that are simpler and easier to synthesize for biological evaluation (see Chart 3 for structures). Specifically, analogues (±)-V2, (±)-V3, (±)-V4, (±)-V5, and (±)-V6, all lacking the C4a hydroxyl group so cumbersome to install, were synthesized (see Supporting Information for their synthesis) and, together with (±)-1, were tested against a number of bacterial strains and compared to natural viridicatumtoxin B [(+)-1, reported values 1 ], natural viridicatumtoxin A [(+)-2, obtained from Professor Yi Tang], minocycline (Minocin, 7), and tigecycline (Tygacil, 9) (see Chart 3 for structures).
Viridicatumtoxin analogue (±)-V2, lacking the C4a hydroxyl group, displayed high potency against the same strains (E. faecalis S613: MIC = 0.5 μg/mL; E. faecium 501: MIC = 0.5 μg/ Figure 6. X-ray-derived ORTEP representation of synthetic viridicatumtoxin B (1). Thermal ellipsoids at 30% probability. Gray = carbon, blue = nitrogen, red = oxygen, green = hydrogen. These results further support the conclusion that the C4a hydroxyl group of the viridicatumtoxin analogues is not necessary for biological activity (see Table 1). Finally, despite the previously reported activity of viridicatumtoxins against several Gram-negative bacterial strains, 1 our tested compounds were inactive against A. baumannii AB210, consistent with previous reports suggesting that the C4-dimethylamino residue is important for imparting the broad-spectrum activity observed for both minocycline (7) and tigecycline (9). 79 Incorporation of such a moiety into the viridicatumtoxin scaffold could expand their antibacterial profile as well as improve their pharmacological properties. 79 In preliminary experiments to probe the mode-of-action of viridicatumtoxin analogues, time-kill assays were performed to measure the killing of E. faecalis S613 by viridicatumtoxin A (2) and (±)-V6 alongside tigecycline (9). The motivation for this study was previous reports that tetracycline analogues with an aromatic C-ring [e.g., viridicatumtoxin A (2) and (±)-V6] act via a bactericidal mechanism as opposed to a bacteriostatic one (i.e., inhibition of the bacterial ribosome). 80 Our time-kill assays clearly indicated that both viridicatumtoxin A (2) and (±)-V6 act bacteriostatically and not bactericidally (see Supporting Information for additional data). 81 Although (±)-V6 did not meet the criterion for bactericidal activity, the ability of (±)-V6 to kill E. faecalis S613 was similar to the clinically used and bacteriostatic antibiotic tigecycline (9). If viridicatumtoxins [i.e., viridicatumtoxin A (2) and (±)-V6] are indeed inhibitors of the bacterial ribosome, as opposed to inhibitors of UPP synthase as suggested by Tomoda and co-workers, 3 then based on the structure of tigecycline (9) bound to the Thermus thermophilus ribosome, the C4−C7 positions of viridicatumtoxins are likely ideal sites for further modifications, as those positions do not directly interact with the ribosome. 82

■ CONCLUSIONS
In summary, we described herein the evolution of synthetic strategies toward viridicatumtoxin B [(±)-1] that finally led to the development of a viable route for the total synthesis of this intriguing natural product. During the course of our studies, we discovered various chemical transformations and acquired synthetic knowledge expected to prove valuable for the synthesis of other fungal tetracyclines and related compounds. In particular, we delineated tactics and strategic requirements for the installation of the hydroxyl groups at C4a and C12a. Other important findings include the anthrone alkylation/ spirocyclization reactions to create the congested EFspirosystem as well as the Michael−Dieckmann cascade reactions that led to the construction of rings A and C. Most notably, our synthetic efforts led to structural revision and configurational assignment of viridicatumtoxin B. Furthermore, application of the developed synthetic technologies allowed the preparation of a number of simpler analogues of the natural product endowed with antibacterial properties comparable with those of viridicatumtoxins A and B, despite their racemic nature. The described chemistry paves the way for further studies, including an asymmetric synthesis of viridicatumtoxins and the design, synthesis, and biological evaluation of other analogues for potential applications as antibacterial agents. 83 ■ ASSOCIATED CONTENT * S Supporting Information Experimental procedures and characterization data for key compounds (pdf and cif files). This material is available free of charge via the Internet at http://pubs.acs.org.