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Incorporation of Large Cycloalkene Rings into Alternating Copolymers Allows Control of Glass Transition and Hydrophobicity

We previously reported that cyclohexene and bicyclo[4.2.0]oct-1(8)-ene-8-carboxamides undergo efficient ruthenium-catalyzed alternating ring-opening metathesis polymerization (AROMP). Here, we demonstrate that cyclodecene and cyclododecene also function as cycloalkene monomers in the bicyclo[4.2.0]oct-1(8)-ene-8-carboxamide AROMP system, thus enabling the synthesis of linear alternating copolymers with spacers of different lengths, as demonstrated by means of NMR spectroscopy and gel permeation chromatography. The glass transition temperature and hydrophilicity of the alternating copolymers decrease as the length of the spacers increases, as determined by differential scanning calorimetry and water contact angle measurements.

R ing-opening metathesis polymerization (ROMP) is widely used for the synthesis of macromolecules. 1−3 This chain-growth technique, in which the driving force of polymerization is the release of ring strain in the cyclic olefin monomers, enables the synthesis of a broad range of polymers with tunable architectures and functions. 4,5 Because the molecular weights of the polymers can be controlled by varying the ratio of initiator to monomers, 6−9 ROMP presents opportunities for synthesizing well-defined and precisely controlled block copolymers 10−13 and graft copolymers 14−17 with unique structures and diverse functions.
During the past two decades, ROMP has been adapted for the synthesis of alternating AB copolymers. The preparation of an AB copolymer requires a monomer that is unable to homopolymerize and that undergoes cross-polymerization efficiently. 18 Therefore, the primary challenge of using ROMP to achieve alternating control lies in matching an appropriate catalyst with a pair of monomers that have alternating affinities for the living metal alkylidene. Schrock et al. synthesized stereochemically controlled alternating copolymers by using molybdenum and tungsten alkylidene initiators. 19,20 However, the resulting polymers contained few heteroatoms, important components for introducing functionality into polymers.
In contrast, by using a ruthenium catalyst with an Nheterocyclic carbene ligand 21,22 we were able to display heteroatom functionality on an alternating carbon backbone while maintaining monomer reactivity, by means of alternating ring-opening metathesis polymerization (AROMP) reactions between 1-substituted cyclobutenes and cyclohexene. 23,24 Importantly, we found that bicyclo[4.2.0]oct-1(8)-ene-8carboxamides and cyclohexene undergo efficient rutheniumcatalyzed AROMP. 25,26 Long, linear, perfectly alternating copolymers are obtained, and the hexyl rings fused to the polymer backbones markedly suppress competing inter-and intramolecular cross-metathesis reactions. Our success with the unstrained monomer cyclohexene suggested that larger cycloalkenes with low strain, such as cyclodecene and cyclododecene, might work equally well in AROMP to provide even larger repeats in the polymer backbone. Different spacer lengths would offer opportunities to vary material properties such as conductivity, hydrophilicity, chain rigidity, thermal properties, 27 and morphology.
Herein, we describe the ruthenium-catalyzed synthesis of alternating copolymers with different spacers using cycloalkene rings (B1−B3) and bicyclo[4.2.0]oct-1(8)-ene-8-carboxamide A as monomer pairs (Figure 1), as the first step to obtaining unique polymer structures with longer repeat lengths between side-chains.
Specifically, polymerization of A and B1 under AROMP conditions in CHCl 3 at 25°C went to completion within 18 h, affording alternating copolymers poly(A-alt-B1) n (n = 10, 30, 50, and 100) after quenching with ethyl vinyl ether (Table 1, entries 1−4). When B2 was subjected to the same conditions, alternating copolymers were also obtained, but the reaction time was shorter (entries 5−8). (Note that the strain energy of B2 exceeds that of B1 by 6.4 kcal/mol. 28 ) It has been reported that in the presence of WCl 6 /Et 2 AlCl and WOCl 4 /Et 2 AlCl coordinate catalyst systems, cis-cyclodecene undergoes polymerization to form trans-polydecenamer. 29 However, B2 did not undergo ROMP under the conditions used in this work (see Supporting Information).
The alternating polymerization reaction of A and B3 was much slower, and achieving 100% conversion was difficult for long polymers. High concentrations of monomer required to achieve 100% conversion were limited by solubility (Table 1, entries 10−12). Commercially available cyclododecene consists of a mixture of trans-and cis-isomers (approximately 30% and 70%, respectively). Upon addition of the catalyst, ciscyclododecene interconverted with trans-cyclododecene, and an equilibrium mixture containing 70% of the trans-isomer and 30% of the cis-isomer was obtained, as determined by NMR ( Figure S5). Importantly, B3 did not undergo homopolymerization under the conditions used for AROMP (see Supporting Information). The reason that AROMP of A and B1 was faster than that of A and B3 but slower than that of A and B2 may be attributable to differences in the reactivities of the alkene-derived carbenes. The order of carbene reactivities is reported to depend on the ease with which the closest double bond in the growing polymer chain can coordinate to the metal center; specifically, the reactivities of carbenes decrease in the order cyclodecene > cyclohexene > cyclododecene. 30 We also carried out the AROMP of A and B2 at a higher temperature (40°C; Table 1, entries 9) and found that dispersity was lower at the lower temperature because intermolecular chain transfer reactions were suppressed. 31 In addition, the incomplete AROMP of A and B3 due to the lower reactivity of B3 derived carbene inevitably yielded broader distributions of polymer lengths.
In the 1 H NMR spectra of poly(A-alt-B1) n , peaks for H1 and H4 appeared in the expected chemical shift regions (δ =  Conversion was determined by monitoring the 1 H NMR spectrum for the disappearance of the amide resonance of monomer A. c Theoretical number-average molecular weight (M n ) calculated from the monomer:catalyst feed ratio. d Determined from GPC using refractive index detection. e Weight-average molecular weight. f Average percentage of double bonds in the backbone of the polymer that were in the E-configuration, as determined from the ratio of H1 and H4 values in the 1 H NMR spectrum. 5.0 and 6.2 ppm, respectively; Figure 2 and Supporting Information). We also observed a signal for H1′, which could not be removed by precipitation or column chromatography of the copolymer. In addition, substitution of vinylene carbonate 32 for ethyl vinyl ether as the quenching agent did not affect the integration value for H1′( Figure S10), and the value remained constant even when the spacer length was AROMP (see Supporting Information). Therefore, H1′ was not due to impurities, polymer terminal groups, or residues resulting from catalyst decomposition. The sum of the integration values for H1 and H1′ was equal to the value for H4. We observed this same proton signal in the NMR spectra of AROMP products in our previous work. 23,25 Comparison of the spectrum of poly(A-alt-B1) n with the spectra of model compounds 2methyl-but-2-enoylamino]-acetic acid methyl esters 33 indicated that different configurations may exist in the conjugated polymer alkenes. This possibility was supported by inspection of the 13 C, HSQC, and HMBC NMR spectra (Figures 2, S9, and S11), which indicated that H1′ is attached to C1′ and that H1 is attached to C1. On the basis of these results, we propose that polymer backbone structures with different configurations were formed, as indicated by the compound labels in Figure 2. The percentage of E-alkene in the polymer backbone was approximately 85% in the AROMP system consisting of bicyclo[4.2.0]oct-1(8)-ene-8-carboxamide and B1, regardless of polymer length. Peaks for H1′ were also seen in the spectra of poly(A-alt-B2) n and poly(A-alt-B3) n , and E/Z ratios of the alkenes in the polymer backbones were similar to the ratio in poly(A-alt-B1) n .
All polymers were stable below 200°C based on thermogravimetric analysis ( Figure S39). Glass transition temperatures (T g ) were determined by differential scanning calorimetry (DSC). T g 's for poly(A-alt-B1) 10 , poly(A-alt-B2) 10 and poly(A-alt-B3) 10 were 11.1, 5.7, and −6.1°C respectively (Figure 3). Based on the reduced transition temperatures, flexibility of the polymer backbone increases when larger   cycloalkene rings are incorporated by AROMP, as expected based on the increased degrees of freedom in the polymer chain.
Water contact angles of polymer thin films prepared through spin coating were measured to evaluate whether hydrophobicity can be controlled by AROMP spacer. The average water contact angle increased as the length of the spacer increased from 6 to 12 carbons (Figure 4). Incorporation of larger spacers results in a lower density of side chain functionality. Increasing the spacing of the hydrophilic amide side chains decreases the interfacial energy and the thin film surface formed becomes more hydrophobic. The frequency of hydrophilic side chain functional groups can be tuned by the length of spacers, thus affecting the hydrophilicity of corresponding alternating copolymers.
To summarize, alternating copolymers with spacers of different lengths were synthesized through rutheniumcatalyzed AROMP. The NMR spectra are consistent with a purely alternating backbone of approximately 85% Econfiguration. The dispersities were acceptable indicating that molecular weights could be controlled. The combination of large cycloalkene monomers and bicyclo[4.2.0]oct-1(8)ene-8-carboxamide provides alternating all carbon backbones with different spacings between heteroatom functionalities on the side chains, leading to copolymers with tunable glass transition behavior and hydrophobicity, as demonstrated by DSC and contact angle measurements.

* S Supporting Information
The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsmacrolett.8b00510.
Experimental details and supporting figures (PDF).


Hydrothermal Conversion of Lipid-Extracted Microalgae Hydrolysate in the Presence of Isopropanol and Steel Furnace Residues

Methods In order to enhance oil yields, the reaction was conducted in the presence of varying loadings of iso-propyl alcohol (IPA) and applied two waste steel furnace residues as potential liquefaction catalysts. Results Primarily, The lipid extraction process needs to be optimized to reduce the amount of acid contaminant within the liquefaction medium. For the HTL process, the addition of 50 vol% IPA resulted in remarkably high oil yields of up to 60.2 wt% on an organic basis, whereas the two furnace residues had no positive effect on the product distribution, and instead favoured the formation of solid reaction products. Nevertheless, the results suggested that the presence of iron potentially reduced the nitrogen and oxygen content of the bio-oil. As such, HTL is a suitable method for valor-ising lipid-extracted algal biomass, where the bio-oil yields can be enhanced substantially by using IPA in conjunction with the water. most research has focused on the extraction of algal lipid, and the subsequent transesterification Abstract Purpose Microalgae have a high potential as a feed-stock for the production of biofuels, either indirectly, through the extraction of lipids, which can be transformed into biodiesel, or directly via whole cell conversion using hydrothermal liquefaction (HTL). Both approaches have disadvantages, due to the high cost of cultivating microalgae with sufficient lipid content (>40%), while the whole cell conversion produces low quality oils, which require significant further upgrading. This work investigated the possibility of realising the benefits of both processes, by studying the liquefaction reaction of a lipid-extracted algae hydrolysate.

Introduction
Microalgae have long been seen as a highly promising feedstock for the production of bio-fuels. They display much higher solar to biomass efficiencies than land-based plants [1], they do not directly compete with food production and can provide secondary functions such as wastewater treatment, carbon sequestration or the expression of valuable into biodiesel [3,4]. Although this technology is relatively well established, it has been estimated that it requires algae with a lipid content of at least 40% at a biomass cost of no more than $0.25 kg −1 to be economically viable [1]. As the expression of algal lipids carries a much higher metabolic cost than proteins or carbohydrates [5], growth rates of lipid-rich algae are significantly reduced, eliminating one of the major advantages of using algae in the first place. Furthermore, the cultivation of pure, lipid-producing algae species requires carefully controlled growth conditions, resulting in significant increases to the cultivation costs [6].
A promising alternative to lipid-based processes is hydrothermal liquefaction (HTL), where the algae are reacted in liquid water close to its critical point. This process forms a crude bio-oil, which can be ultimately processed into conventional transportation fuels. As HTL converts the entire algae, not just lipids, it can utilise much cheaper and faster growing algae. In addition, it may facilitate the recovery of nutrients, such as nitrogen and phosphorus, to the aqueous phase, facilitating recycling for further algae growth, or allowing it to be used as a liquidbased fertilizer. However, the bio-oil has a much lower quality than lipids, containing high concentrations of oxygen and nitrogen, and poor flow properties. Consequently, significant further upgrading is required before the oil can be fractionated into fuels.
A potential way of realising the benefits from both processes could be the combination of algal lipid production with subsequent HTL of the remaining biomass components. In this way, the production of the lower value HTL oil could help to offset the production costs for the higher value lipids, and may allow the use of faster growing algae with lower lipid content, compared to a solely lipid-based process.
The lipid-extracted algae would be expected to consist predominantly of protein and carbohydrate derived material, which tend to yield significantly lower HTL yields than lipids [7,8]. Consequently, it may be desirable to conduct the HTL of this algae residue in the presence of polar organic solvents, such as ethanol, acetone, or ethylene glycol, or organic waste products, such as glycerol, which have been previously found to enhance bio-oil yields, compared to water-only reactions [9][10][11][12]. These enhancements have been generally associated with reduced critical temperatures and pressures allowing the use of milder reaction conditions and lower dielectric constants, helping to dissolve high-molecular-weight products [13]. It has also been suggested that the hydrogen donation abilities of certain solvents such as ethanol could help to improve the quality of the final bio-oil [14].
The highest bio-oil yields, ranging from 51.4 to 57.0% were obtained in the highly polar solvents ethylene glycol, ethanol, acetone and ethyl acetate, significantly exceeding the yields obtained in water (43%). Further optimization of the reaction conducted in ethanol produced a bio-oil yield of 65.1%, however the overall product recovery exceeded the organic content of the algae, indicating consumption of the reaction solvent. Similar findings were made during the conversion of Chlorella pyrenoidosa in acetone, which achieved remarkable bio-oil yields of 78.9% at 290 °C, but with an overall product recovery of 106.9% [10]. In addition, contrary to water liquefaction, the resulting oil contained a higher nitrogen content than algae.
HTL in pure solvent also requires complete biomass drying, incurring significant energy penalties, and results in low critical temperatures, restricting the maximum allowable reaction temperature. For these reasons, HTL in pure solvent may be undesirable. Instead, the liquefaction could be conducted in solvent-water mixtures, which were often found to produce higher yields than the reactions in the pure solvent [10,12,13,15]. In these studies, the optimal solvent-water ratios were found to be strongly dependent on both the solvent, and the selected reaction temperature, and it was suggested that the optimal solvent concentration is governed by the proximity of the critical temperature of the mixture to the reaction temperature [13]. However, for polar solvents, the subsequent recovery of the bio-oil and reaction solvent may be more challenging, compared to conventional HTL reactions, due to the high miscibility of the solvent with the aqueous phase. Furthermore, only few solvent-water combinations have been studied to date, and to the best of our knowledge have not yet been applied to the conversion of lipid-extracted algal residue. One of the solvents that appears relatively neglected for these reactions so far is isopropanol (IPA), despite its known ability to act as a hydrogen donor for transfer hydrogenation reactions, [16,17] potentially helping to improve the quality of the resulting bio-oil, and consequently has been selected for this study.
Another method of enhancing bio-oil yields is the addition of catalysts. Liquefaction yields from the conversion of carbohydrates were found to be positively influenced by the addition of the base catalyst sodium carbonate (Na 2 CO 3 ) [7], whereas the noble metal catalysts Pd/C and Pt/C were found to produce significantly enhanced bio-oil yields with improved flow properties and a reduced nitrogen content [18,19]. Despite this, due to their high cost, the application of noble metal catalysts for large-scale HTL of algae is unlikely to represent an economically feasible solution. Therefore, alternative, low-cost materials need to be identified that can be added to the liquefaction medium instead.
There is some evidence that lower cost materials, such as FeSO 4 [20], or Fe 3 O 4 can work to enhance bio-oil yields [21]. One source of inexpensive material is furnace residues or furnace slag. This material is a mix of Fe, Ca, Mg, Al and Si oxides, that can be added to a reactions to impart a low cost catalytic effect while not being valuable enough to need to be recovered and purified. These materials have been demonstrated to impart a positive effect on processes such as gasification [22], catalytic cracking of triglycerides [23], hydrogen production [24] or degradation of dye molecules in water [25].
In this investigation, the possibility of combining HTL with algal lipid production was evaluated by upgrading an algal cake, recovered from a lipid-extracted algae hydrolysate solution, using the HTL process. The reactions were conducted both in pure water, and in the presence of varying concentrations of isopropanol (IPA), in an attempt to enhance the overall bio-oil yields. In addition, the effect of adding two steel furnace residues on the product distribution obtained from the conversion of the algae cake was evaluated.

Algae Cultivation and Lipid Extraction
Lipid-rich algae (Chlorella sp. strain, obtained from the Canadian Phycological Culture Centre) was produced under continuous flow, at a biomass concentration of 30 g L −1 , applying heterotrophic conditions, using glucose as the carbon source, as described elsewhere [26].
Solvent extraction of algal lipids is reviewed fully elsewhere [27]. In this investigation, prior to lipid extraction, the algae slurry was mixed with 0.1 g H 2 SO 4 for each gram of dry cell weight (dcw) and hydrolysed in a PHOENIX AV100 Plus autoclave at 120 °C for 1 h. Subsequently, the lipids were extracted using a 1:4 mixture of ethanol and hexane at a ratio of 2.5 mL g −1 (dcw). The solvent/algae mixture was stirred for 1 min and allowed to rest until spontaneous phase separation occurred, followed by the recovery of the top phase containing hexane and lipids. This procedure was repeated four more times (using hexane only) and the recovered hexane-lipid phase was separated by evaporation under vacuum.

Hydrolysate Work-Up
Following lipid-extraction, the algal hydrolysate was centrifuged to remove any residual hexane and to reduce the water volume by 43%. Subsequently, the remaining biomass was neutralized using a 5 M sodium hydroxide solution, followed by a second centrifugation step to obtain a wet paste with a water content of 72 wt%. Finally, the cake was oven-dried at 70 °C, and finely ground prior to the HTL reaction.

Reactor Set-Up
Hydrothermal conversion reactions of the dry algal cake were conducted in a 100 mL Parr reactor (series 4560 mini reactor system). Each reaction converted 5 g of biomass, in the presence of a total of 50 mL of reaction solvents, consisting of varying concentrations of deionized water and/ or IPA. Catalytic reactions employed an additional 5 g of finely ground material, either LFR or EAFS.
Prior to reaction, the reactor was carefully sealed and charged with 10 bar nitrogen to reduce the vaporisation of the reaction solvent. Reactions were stirred at 180 rpm, and allowed to proceed for 1 h, once the desired reaction temperature (220 or 310 °C) had been reached. Reactor pressures ranged from 22 bar for reactions at 220 °C in pure water to 132 bar at 310 °C and an IPA loading of 50 vol%. Following reaction, the heating mantle was removed and the reactor was allowed to cool down to room temperature (<40 °C). Heating times ranged from 56 to 71 min, whereas reactor cooling required between 90 and 120 min, giving a total contact time between reagents and catalysts of up to 4 h.

Product Recovery
Following the reaction, the gases were vented, the reactor was opened and the reaction products were vacuum filtered through pre-weighed filter paper. This allowed the recovery of the aqueous product phase. A small aliquot of this phase was oven-dried overnight at 70 °C to determine the yield of the water phase residue. Subsequently, the reactor and filter paper residue were washed with chloroform, until the solvent remained clear.
In order to extract IPA and water-soluble organics from the water phase, the aqueous and solvent phases were recombined and thoroughly mixed prior to gravity separation. To determine the impact of this additional processing step, baseline reactions were conducted at 310 °C with IPA loadings of 0 and 50% (v/v), without recombination of the aqueous and solvent phases.
Following recovery of the oil phase, the solvent was removed at 60 °C and under vacuum (120 mbar), before further separating the bio-oil product into a heptane soluble (light-oil) and chloroform soluble (heavy-oil) fraction. The filter paper was dried at 70 °C overnight, before determining the solid product weight. For reactions involving catalysts, the catalyst weight was subtracted before calculating the solid yield.

Product Analysis
Algae Analysis CHNS analysis of the algae cake was carried out on a vario MACRO cube analyser, Elementar (Hanau, Germany), with a combustion tube temperature of 1150 °C and a reduction tube temperature of 850 °C.
Thermogravimetric analysis (TGA) of the press cake was carried out by on a TGA/DSC1 analyser by METTLER (Zürich, Switzerland), using a ramp rate of 10 °C min −1 to a maximum temperature of 700 °C.
The total organic carbon (TOC) and total nitrogen (TN) contents in the 'acid water' and 'neutral' water were analysed using a Shimadzu TOC/TN analyzer, at dilutions of 2 vol%.
The glucose content in the 'acid water' and 'neutral water' phase was determined using a commercial enzymatic glucose-oxidase assay kit by Bioliquid® using tenfold dilution.

HTL Reaction Products
In addition to the CHNS analysis, the composition of the solid reaction products was also determined using SEM-EDX analysis. Prior to analysis, the samples were gold coated using a MITECH Sputter Coater, Model K450 (Kent, United Kingdom). The samples were then analysed using an LEO Electron Microscope (Cambridge, UK), model number Leo 440i using a 6070 EDX detector. The accelerating potential was 20 kV with a beam current of 600 pA. Elemental composition was determined from an average of 10 different area scans.
To evaluate the degree of catalyst leaching into the aqueous and bio-oil phases, the 'elemental retention' of catalytic material in the solid phase was calculated using following formula: where X i Elemental content of elemental i and m mass. Subscripts P, C and A stand for product, catalyst and algae, respectively.
Error Analysis The reactions conducted in pure water were carried out in duplicate, and are represented as averages, together with their standard deviation. Whilst the catalytic reactions are based on single-data points, the error is based on the maximum deviation obtained from the wateronly reactions. Errors for the elemental retention of catalytic materials to the solid phase are based on the standard deviation of the ten separate area scans, during the SEM-EDX analysis.

Biomass Preparation
The overall process flow diagram for producing fuels and products from algal biomass is given in Fig. 1. The lipid extraction process employed harsh hydrolysis conditions, using 0.1 g H 2 SO 4 per gram of biomass, to help break up the biomass. Because of this, significant work-up was required to neutralize the lipid-extracted algae hydrolysate before the algae residue could be applied to the HTL reaction.
First, the hydrolysate was centrifuged to remove any residual hexane and reduce the water volume by 43%. Fig. 1 Schematic demonstrating the production of lipid and HTL bio-oil from one algal source. The grey lines indicate the lipid extraction stages, the black the HTL processing. The dashed line indicates that in an industrial process there would be no need for drying the wet cake Subsequently, the acid slurry was neutralized using a 5 M NaOH solution, followed by a second centrifugation step to recover a wet biomass cake, containing a residual water content of 72%. Finally, to facilitate lab-scale processing, this cake was dried at 70 °C and finely ground prior to the HTL reactions. However, this step was solely conducted to facilitate the accurate determination of the algae reaction weight and allow algae analysis in our study, and would not be required in an industrial set-up.
The resulting algae cake contained a remarkably high ash content of 52.9%, which could be partially attributed to the high amounts of sulphuric acid and sodium hydroxide used in the lipid extraction and algae cake recovery process. Based on the quantity of sodium hydroxide added to neutralize the hydrolysate solution, sodium sulphate (Na 2 SO 4 ) was estimated to account for 33.0% of the total algae cake weight, or 62.4% of its ash and 85.6% of its sulphur content. The remaining ash content could be attributed to micronutrients, including calcium chloride, magnesium sulphate and potassium phosphates, as well as oxidation products from the reaction of sulphuric acid with the algae itself, such as ammonium sulphate, and carbonates produced during the cultivation of the algae.
Whilst the dewatering and neutralization of the hydrolysed algae residue was necessary to produce a suitable HTL feedstock, it also resulted in a significant loss of organic biomass components. Using the results from the total carbon and nitrogen analysis of the water phase, it was possible to calculate the elemental distribution to the dry cake and the two recovered water phases (Table 1). This analysis shows that only 44% of carbon and 39% of nitrogen from the lipid-extracted hydrolysate were recovered into the dry cake, whereas the remainder was lost during the two centrifugation steps. 11.6% of the carbon content in the neutral water and 14.6% of the carbon content in the acid water could be directly attributed to glucose, one of the main products from the hydrolysis of carbohydrates. Therefore, this sugar accounted for 7.5% of the total carbon in the hydrolysate and could potentially be recycled back for further algae cultivation.
The sulphur phase was predominantly associated with the sulphuric acid present in the hydrolysate. Even though a large amount of sulphur was lost during the two centrifugation steps, just under a quarter (24.5%) of the total sulphur was retained within the dry algae cake.

Optimization of Product Recovery Method
Prior to investigating the detailed relationship between IPA loadings and bio-oil yields, the effect of the product recovery method was investigated at 310 °C (Fig. 2). The results show that the bio-oil yields were significantly enhanced when the aqueous phase was extracted with chloroform. For HTL in pure water, the bio-oil yields more than doubled from 6.6 to 13.4 wt%, predominantly associated with an increase in the light, heptane-soluble oil fraction. An even bigger effect was observed for the reaction conducted in the presence of 50 vol% IPA. Without water extraction, the total bio-oil yields amounted to only 2.4 wt%, but increased to 28.3 wt% after the aqueous phase was washed with chloroform, a more than 10-fold increase.
The differences between the water-only and the 50 vol% IPA reaction can be associated with the high polarity of IPA, causing it to partition to the water phase, together with dissolved bio-oil components. Even in the absence of IPA, the difference in bio-oil recovery with and without chloroform washing of the water phase was significantly higher than the 20 or 30% previously reported in the literature [28]. This could be related to the complete absence of lipid-derived products from the algae cake used for this study, and a higher proportion of smaller, more polar organics, produced from the carbohydrate and protein fractions.
Whilst for conventional HTL there is currently no consensus whether the increase in bio-oil yields justifies the additional costs of the solvent extraction procedure [29], it is clear that the HTL of the algae cake in the presence of IPA appears to be only feasible if the water phase is subsequently extracted to recover IPA and the organic reaction products. Consequently, this approach was applied to all subsequent experiments. While washing with a lipophilic solvent such as CHCl 3 allows extensive recovery of the bio-oil fraction, it is unclear whether this would be feasible on an industrial scale. Similar biofuel production processes recycle the finished fuel as a solvent, or use fossil fuels to increase the yield of the final product [4]. Undoubtedly, whatever solvent is used to increase bio-oil yields from the water phase would need to be recycled efficiently, with the IPA, in an industrial process.

Effect of IPA Concentration on Product Distribution
In this study, the maximum IPA loading in the reactor was limited to 50 vol%. Firstly, 50 vol% was seen to be the maximum feasible concentration that could be employed industrially, without requiring excessive biomass drying, and losing one of the major advantages of the HTL process. Secondly, the applied reaction temperature of 310 °C corresponds to the critical temperature of a 40 vol% IPA-water solution, as predicted using the Peng-Robinson equation of state within Aspen Hysys®. Operating at 50 vol% IPA therefore ensured that the maximum possible IPA concentration in the liquid phase was achieved, without causing excessive evaporation of IPA, resulting in operation above the design pressure of the reactor. Finally, results from previous HTL studies conducted in water-solvent mixtures suggested that maximum bio-oil yields were obtained when the critical temperature of the mixture corresponded to the reaction temperature [13].
When increasing the IPA content of the reaction solution from 0 to 50 vol%, both the light and heavy bio-oil products were found to increase steadily (Fig. 3a). However, the increase in the light oil phase (5.4 to 17.6 wt%) was much more pronounced than that of the heavy biooil (8.0 to 10.7 wt%). At the same time, the solid yields remained almost constant (between 4.0 and 4.7 wt%), whereas the water residue yields reduced significantly from 47.7 to 30.8 wt%. Consequently, the overall product recovery remained relatively constant (ranging from 63 wt% at 50 vol% IPA to 70 wt% at 10 vol% IPA, with the unaccounted fraction likely to be gas product), suggesting that IPA helped to convert or transfer some of the water-soluble products into the bio-oil phase, without being incorporated into the bio-oil itself. This is contrary to previous liquefaction experiments using alcohols, where the product recoveries exceeded the initial amount of biomass added [9,10]. However, in these cases, the alcohols appeared to react with the lipid fraction to form esters, whereas the algae press cake used in the present study contained only negligible amounts of residual lipids.
Compared to previous studies using ethanol-water solvent mixtures, commonly seen as the best solvent and which reported maximum oil yield increases of around 25% at optimized ethanol-water ratio, compared to the water-only reactions [13,30], the increase in total oil yields obtained in the present study (111%) is highly promising. However, it should be noted that due to the nature of the feedstock, the final yields of 28.3% are still significantly below the yields obtained in the cited studies (57.3 and 63%), and therefore it is not possible to distinguish between the effect of the solvent (IPA vs. ethanol) or the differences in the feedstock.
Similar conclusions can be drawn from the elemental balance to the different product phases (Fig. 3b). Whilst the carbon retention in the solid remained relatively constant, the carbon, hydrogen and nitrogen distribution to the light oil phase increased significantly. The increase in bio-oil nitrogen content matched the reduction of nitrogen that partitioned into the aqueous phase, as calculated from the total  Fig. 3 Effect of IPA concentration in liquefaction medium for reactions at 310 °C on a overall product distribution, of solids, heavy oil, light oil and the residue from the aqueous phase, b elemental distribution to different product phases nitrogen analysis of this phase, suggesting a direct transfer of nitrogen containing compounds from the aqueous to the bio-oil phase. (It should be noted that the presence of residual solvent in the water phase made it impossible to obtain meaningful results for the total carbon analysis of the water phase.) When considering the ash content of the algae cake used for this study (53 wt%), it is remarkable that the solid fraction amounted to only 4 to 5 wt% of the total reaction products. This confirms that a major portion of the ash consisted of highly water-soluble material (mostly sodium sulphate), which was recovered into the aqueous product phase instead. For the reaction in 50 vol% IPA, the combined solid and water phase residue yields amounted to 35 wt%, only just above the estimated sodium sulphate content in the biomass (33 wt%), but significantly less than the total algae cake ash content. Consequently, a significant fraction of the ash appears to have been lost to the gas phase, or volatile water-soluble products, potentially through the thermal decomposition of carbonates into carbon dioxide, or the release of ammonia from ammonium salts.
Given the high ash content of the algal press cake, the bio-oil yields of 28.3 wt% obtained for an IPA loading of 50 vol% correspond to a high recovery of the organic fraction of 60.2 wt%, significantly higher than the yields typically expected from the HTL of proteins (11-18%) or carbohydrates (6-15%) [7].
Despite these encouraging findings, it should be noted that the recovered algae cake accounted for only 43.6 wt% of the total carbon present in the lipid-extracted hydrolysate, and consequently the overall carbon recovery amounted to only 24.6%. Attempts were made to recombine the algae cake with the 'neutral water' phase, recovered from the second centrifugation step, however the reaction resulted in the formation of hard solid deposits. These were presumably formed from the high loadings of sodium sulphate present in the neutral water phase, and would be expected to cause significant operational problems within an industrial setting. Consequently, the lipid extraction process needs to be improved to reduce, or eliminate, the amount of sulphuric acid required and facilitate the recovery of the lipid-extracted hydrolysate. Potential options are the use of higher hydrolysis temperatures, which would allow the use of lower acid concentrations, the use of solid acid catalysts, or the selection of algal strains with weaker cell walls, allowing the application of milder hydrolysis and lipid extraction conditions. Ideally, the entire lipidextracted hydrolysate would be processed directly, without further work-up, although the recovery of sugars and other nutrients may be desirable to allow their recycling for further algal growth.
Provided that these issues are addressed, it is expected that the combined lipid production and HTL of algae residues is significantly more favourable than algae lipid production on its own. Current estimates for lipid production costs from microalgae have been found to range widely [31], from around $1.9 L −1 for microalgae grown on wastewater [32] to $3.36 L −1 for an open pond, commercial size algal biofuel facility located in Southwest USA [33], significantly above currently acceptable fuel costs. Similarly, estimates of the environmental burden and energy returns on investment have given widely disparate results, due to different modelling assumptions [34]. Yuan et al. estimated a minimum lifecycle energy requirement of 2.35 MJ for each MJ of algal biodiesel and green house gas emissions of 143 g (CO 2 ) MJ −1 , although these figures were reduced to 1.02 MJ MJ − 1 and 71 g(CO 2 ) MJ −1 when applying anaerobic digestion (AD) to the lipid-extracted residue [35]. Only slightly better results were obtained by Clarens et al., who calculated energy returns of investment ranging from 0.65 to 1.13 for a biodiesel production process, combined with AD, depending on the source of carbon and other nutrients [36]. In contrast, Stephenson et al. estimated a fossil-energy requirement reduction of 85% and global warming potential reduction of 78% compared to fossilderived diesel [37]. In all these studies, the major contributors to the overall lifecycle burden are the algae cultivation and pretreatment steps, and therefore further energy recovery of the lipid-extracted algal residue is highly beneficial. Algal biofuel production via HTL appears more beneficial as demonstrated by a previous life-cycle analysis giving an energy-return-on-investment of a full-scale process comparable to fossil-derived diesel and gasoline, whilst the greenhouse gas emissions were reduced to less than a third [34].

HTL in the Presence of Steel Furnace Residues
In an attempt to increase the bio-oil yields from the liquefaction of the algae cake, and potentially lower the reaction temperatures, the reaction was conducted in the presence of two steel furnace residues, ladle-furnace residue (LFR) and electric-arc furnace slag (EAFS). LFR consisted predominantly of calcium and silicon oxides, with smaller quantities of carbon, magnesium and titanium. EAFS also contained significant amounts of calcium and silicon oxides, magnesium and carbon, but also contained significant fractions of iron, aluminium, manganese and titanium. Consequently, both materials could potentially enhance basecatalysed reactions, whereas EAFS could also be active for iron-catalysed cross-coupling or hydrogenation reactions.
It should be noted that the objective of the present study was to evaluate the overall catalytic activity of these materials for the conversion of the lipid-extracted algae cake as well as testing their stability under HTL conditions. Both materials are waste products, and therefore available at low cost, although industrial processing may be limited by the additional energy requirements of heating these materials to reaction temperature and suspending them within the reaction stream. A potential way of addressing these limitations would be to conduct the HTL reaction in two stages, by combining a continuous stirred tank reactor (CSTR) with a plug flow reactor, similar to the configuration employed by Elliott et al. [38]. The catalyst could then be retained within the CSTR, whilst the reaction continues within the secondary plug flow reactor.
As in the previous section, reactions were conducted at 310 °C, in pure water and 50 vol% IPA, however, additional reactions were carried out at 220 °C. As this temperature is below the critical temperature of pure IPA (235.1 °C), the reactions were also conducted in pure IPA, as well as in 50 vol% IPA and in pure water.

Catalyst Stability
An important consideration for using catalysts is the ease with which they can be recovered and recycled after the reaction. Consequently, heterogeneous catalysts are generally preferred over homogeneous catalysts, however they must be stable during the reaction conditions to prevent the loss of catalytic material into the product phases, resulting in potential contamination issues. To assess the suitability of the two steel furnace residues for the HTL of algae press cake, the study tracked the recovery of the major elemental components in the solid phase.
LFR Apart from oxygen and carbon, which were not tracked as they are also present in the biomass itself, LFR contained significant quantities of calcium (34.6 wt%), silicon (11.1 wt%) and magnesium (1.5 wt%), shown in Table 2.
During the reaction at 220 °C in pure water, substantial amounts of silicon and magnesium were lost, whereas calcium retention was close to 80% (See supporting information). Following the introduction of IPA to the reaction medium, the silicon and magnesium recovery increased significantly, with the calcium recovery appearing to drop slightly. The reverse trend was observed at 310 °C. At this temperature the recovery of silicon and magnesium in pure water was significantly enhanced, compared to the reaction at 220 °C, but dropped for the reaction conducted in the presence of IPA.
The data suggests that under HTL conditions, all three elements display high solubility in the water phase. Calcium oxide is known to react with water to form calcium hydroxide, whereas the solubility of magnesium oxides in water increases with increasing temperature. Amorphous silicon dioxide in turn was previously found to display a maximum water solubility of 1660 mg kg − 1 at a temperature of 340 °C [39]. A potential explanation for the enhanced retention of calcium, compared to silicon and magnesium, is its higher concentration within LFR, resulting in full saturation of the water phase. Consequently, the recovery of silicon and magnesium increased following the introduction of IPA, as the volume of water decreased. However, it is clear that this correlation broke down at the higher reaction temperature of 310 °C and suggests that the catalyst underwent chemical changes to modify its solubility in the reaction medium. Calcium could have reacted with the sulphates in the press cake to form gypsum, whereas silicon and magnesium could have been reduced or adapted more stable configurations. The overall water loading and the presence of IPA would have been expected to influence the equilibrium between these reactions, ultimately affecting the final product phase distribution.
In all cases, it should be noted that the data contains a large degree of uncertainty. Apart from the general limitations of using SEM-EDX, this may be related to a nonuniform composition of the solid reaction product as well as the difficulty of fully recovering solid precipitates from the reactor walls.
Despite its low concentration, within error, titanium appeared to have been fully recovered into the solid phase at all reaction conditions (see supporting information). The recovery of magnesium and silicon appeared to be increased, compared to LFR, whereas the calcium recovery remained about the same. Iron recovery at 220 °C did not follow a clear trend, reaching around 100% for an IPA concentration of 50 vol%, but recoveries of only 55% in pure water and 42% in pure IPA. The manganese recovery followed a similar trend to iron, remaining around 60% at all conditions, apart for the 50 vol% IPA loading at 220 °C, whereas the aluminium recovery remained consistently above 80%. The low recovery of iron and manganese for the reaction in pure IPA is surprising, as only three product phases (solid, oil and gas) were collected from the reactor. A potential explanation could be the formation of iron and manganese deposits on the reactor walls, which were difficult to recover after the reaction. Consistent with the results for LFR, the calculated elemental recoveries showed a high degree of experimental uncertainty. Whilst EAFS appeared to display a higher overall stability than LFR, the reaction still caused a significant portion of magnesium, silicon, iron and manganese to partition into the water phase. Particularly the dissolution of manganese to the water phase could be problematic, due to its known neurotoxicity at elevated groundwater concentrations [40].

Product Distribution
Compared to the water-only reactions, at 220 °C, the addition of 50 vol% IPA to the reaction medium resulted in a significant increase in the bio-oil yields obtained from both the catalytic and the blank reactions (Fig. 4a). Solid yields remained relatively constant, whereas the water phase residue yields experienced a significant drop, together with a reduction in the overall mass balance closure, indicating enhanced gas formation.
Conducting the reactions in pure IPA resulted in only a small further increase in bio-oil yields, compared to the reactions in 50 vol% IPA. No water phase was present for these reactions, and consequently no water phase residue was recovered. In contrast, the solid yields increased significantly from less than 20 wt% at an IPA loading of 50 vol% to up to 45.7 wt% for the reaction catalysed with LFR. This large increase can be attributed to the high quantity of water-soluble ash present in the algae cake. For reactions conducted in water, these salts were dissolved and subsequently removed together with the aqueous phase (and precipitated as water phase residue), but in the absence of water, the salts remained part of the solid product fraction. Even so, the maximum solid recovery of 45.7 wt% remained below the total ash content of the algae cake (52.9 wt%), suggesting that during the reaction, some of the inorganic press cake components were either dissolved into the IPA phase, or decomposed into gas phase products.
For the reactions at 220 °C, the presence of LFR and EAFS did not appear to have a positive effect on the product distributions obtained from the HTL of the algae cake. In pure water, the overall bio-oil yields were significantly lower for the reactions involving the two catalysts (6.0 wt% for LFR and 5.7 wt% for EAFS), compared to a yield of 8.4 wt% for the non-catalytic reaction. Following the introduction of IPA, the difference became less pronounced, but maximum overall oil yields of 14.5 wt% at an IPA loading of 50 vol% and 18.8 wt% in pure IPA were still obtained for the non-catalytic reactions. However, compared to the blank run, EAFS did appear to slightly favour the formation of light, hexane-soluble oil.
The solid yields obtained in the presence of EAFS were comparable to the non-catalytic reaction at all three IPA loadings, whereas the yields obtained with LFR were significantly enhanced for the reactions conducted in the presence of IPA. Even though the solid yield enhancement appeared to be less significant in pure water, it should be noted that at this condition, a significant amount of catalyst  At 310 °C the difference between the blank and catalytic runs was much more noticeable than at 220 °C (Fig. 4b).
Both catalysts resulted in a significant reduction in bio-oil yields, and a large increase in solid yields, particularly in the presence of 50 vol% IPA. All water residue yields in pure water were approximately equal, but for the non-catalytic runs, the yields reduced significantly from 47.7 wt% to 30.8 wt% following the addition of 50 vol% IPA, whereas they remained unchanged around 41 wt% to 49 wt% for the catalytic reactions. This suggests that the presence of IPA helped to transfer organics from the water-phase into the bio-oil, whereas the catalysts increased the formation of solids, potentially through enhanced coke or char formation on the catalyst surface. This is consistent with previous work that has shown that the presence of inorganic salts, including potassium carbonate and calcium acetate, can enhance secondary char formation during the pyrolysis of lignocellulosic biomass [41,42]. The presence of these metals appeared to reduce the pyrolysis temperature of the biomass, as well as influencing the relative rates of polymerization and cracking reactions of intermediate decomposition products to favour either char or gas formation [43]. Whilst pyrolysis reactions are mostly limited to the solid and gas phase, secondary HTL reactions proceed within the aqueous phase. Consequently, higher salt concentrations may be required to have a similar effect, resulting in higher char yields in the presence of calcium-rich LFR, compared to the more stable EAFS.
Comparing the results obtained for the non-catalytic reactions shows that the overall bio-oil yields obtained at 220 °C in the presence of 50 vol% IPA (14.5 wt%) and pure IPA (18.8 wt%) were higher than the bio-oil yields obtained at 310 °C and in pure water (13.4 wt%). The same trends were observed for the bio-oil yields obtained from the catalytic reactions, however it should be noted that the reactions at 310 °C, in 50 vol% IPA, always yielded the highest oil yields of all conditions studied. Solid yields, in turn, were lower for both non-catalytic reactions at 310 °C, than for any of the reactions at 220 °C, but the opposite trend was observed for the reactions involving LFR and EAFS.

Elemental Recovery
To study the effect of reaction temperature, IPA content and the presence of the catalysts in more detail, the distribution of carbon, hydrogen and nitrogen to the oil and solid phases was calculated for the reactions in pure IPA at 220 °C and the two reaction conditions at 310 °C, in pure water and with a 50 vol% IPA loading (Fig. 5).
Consistent with the higher oil yields obtained at 220 °C in pure IPA, compared to the reaction at 310 °C in pure water, the carbon and hydrogen recoveries to the oil phase for the non-catalytic reactions were slightly increased from 32.8 to 34.9% and 20.2% to 29.5%, respectively. At the same time, the nitrogen recovery was significantly elevated from 21.0 to 35.6%, resulting in an overall increase in the nitrogen content of the bio-oil from 4.7 to 6.6 wt%. Similarly, the contribution of the unaccounted elemental fraction (predominantly oxygen) to the overall oil yield increased from 13.5 to 18.8 wt%.
The addition of 50 vol% IPA to the reaction medium at 310 °C resulted in a large increase in the total carbon and hydrogen recovery to the bio-oil, and was comparable to the simultaneous increase in nitrogen recovery, resulting in similar overall nitrogen contents in the oil of 4.7 wt% for the reaction in pure water and 4.8% in the presence of IPA. Despite this, the increased retention of carbon, hydrogen and nitrogen could not account for the entire increase in oil yields, resulting in an increase in the unaccounted elemental fraction from 13.5 to 19.0 wt%. The H/C mass ratio only increased marginally from 0.118 to 0.121, suggesting limited transfer of hydrogen from IPA to the bio-oil. This is not surprising, as this reaction generally requires a good hydrogenation catalyst, such as RANEY® Nickel. Carbon recoveries to the solid phase were significantly higher at the lower reaction temperature (43.3%) than at 310 °C (8.8% in pure water and 6.4% in 50 vol% IPA), consistent with the differences in solid yields and the absence of a water phase residue for the reaction in pure IPA. Slightly different trends were obtained for the reactions over the two catalysts. Whilst the highest carbon and nitrogen recoveries were still obtained at 310 °C and in the presence of IPA, the hydrogen recovery was reduced compared to the reaction at 220 °C, and the nitrogen content in the bio-oil was higher for the reaction in pure water. The carbon recovery to the solid phase at 310 °C was also decreased in the presence of 50 vol% IPA, contrary to the simultaneous increase in solid yields. This suggests that the increase in solid yield can be mostly attributed to reduced dissolution of inorganic material from the algae cake and the catalysts, whilst IPA helped the recovery of organic carbon into the bio-oil.
These findings show that the addition of IPA to the liquefaction medium can result in a significant enhancement of liquefaction yields, potentially allowing the reaction to proceed under much milder conditions compared to the pure water-phase reaction. However, the bio-oil quality obtained at 220 °C was also reduced, as indicated by an increased oxygen and nitrogen content. This was probably the result of incorporating lighter, water-soluble organics into the bio-oil, which contained high concentrations of oxygen and nitrogen. Furthermore, the reaction in pure IPA is unlikely to be feasible for industrial scale production, as it requires highly energy-intensive drying steps to obtain the fully dried biomass. In contrast, liquefaction in the presence of 50 vol% IPA at 310 °C offered a good compromise between enhanced oil yields and reduced biomass drying. Whilst it caused a reduction in the carbon and hydrogen content in the bio-oil, due to the increased incorporation of oxygen, it only had a limited impact on the nitrogen content from the non-catalytic reaction, and even reduced the nitrogen content for reaction with the two furnace residue materials.
Neither of the two catalysts employed for this study resulted in an increase in bio-oil yields. LFR in particular caused an increase in solid yields and decreased the biooil yields without having any obvious beneficial impact on bio-oil composition. It was previously hoped that the basic nature of the catalysts would help the conversion of carbohydrates into bio-oil products. However, it is possible that the high ash content of the microalgae cake itself (53 wt%) provided sufficient quantities of base catalyst, and therefore the presence of LFR had no further beneficial impact. EAFS also reduced bio-oil yields and increased solid content, but had a beneficial impact on the carbon and hydrogen contents of the bio-oils obtained by the reaction, related to a reduced oxygen concentration. This suggests that the iron catalysed the deoxygenation reaction, however as iron only accounted for a small fraction of the overall catalyst material (12.6 wt%), the beneficial impact was outweighed by the reduction in bio-oil yields.

Conclusions
Combining algal lipid production with HTL is a promising method for realising the benefits of both methods, of producing higher value fuels at lower overall production costs. Both the production of lipid heterotrophically and the hydrothermal liquefaction of algae have been demonstrated on the pilot scale separately previously, however, the two processes would need to carefully integrated, to prevent contamination of the algae residue during the lipid extraction stage, and optimize the lipid-content of the algae to achieve the best compromise between product yields and cultivation costs.
Conducting the HTL of lipid-extracted algae in the presence of 50 vol% IPA resulted in remarkably high recoveries of the organic fraction to the bio-oil phase of up to 60.2 wt%, significantly above the oil yields typically expected from carbohydrates or proteins. It also allowed the application of much lower reaction temperatures, to obtain comparable oil yields to a water-only reaction, albeit with a significant reduction in bio-oil quality, as a result of increased nitrogen and oxygen incorporation. However, this approach proved only feasible if the water-phase was extracted with a non-polar solvent, to allow the recovery of IPA and light organic products. Therefore, further studies including cost-benefit analyses are required to assess the feasibility of the overall process.
The addition of two steel furnace residues, LFR and EAFS, as potential liquefaction catalysts, appeared to have no beneficial impact on bio-oil yields, but instead resulted in a significant increase in solid yields, potentially due to the formation of char and coke over the catalyst surface. At the same time, the liquefaction reaction resulted in significant leaching of calcium, silicon, magnesium, iron and manganese to the water phase. In particular, the loss of manganese could be a major problem due to its known neurotoxic effect. Nevertheless, the presence of EAFS had a positive effect on the carbon, hydrogen and nitrogen contents of the bio-oils produced in 50 vol% IPA, suggesting that it could have a low activity towards deoxygenation and denitrogenation reactions, most likely catalysed by its iron content of 12.6 wt%. Future work could therefore explore the use of more stable, iron-based catalysts, to obtain more favourable bio-oil compositions.


extfieldsofstudy: Chemistry Medicine

s2fieldsofstudy: Chemistry Biology

text:
The effect of geometric isomerism on the anticancer activity of the monofunctional platinum complex trans-[Pt(NH3)2(phenanthridine)Cl]NO3

Initially the development of platinum-based anticancer agents was focused on the synthesis and evaluation of complexes that obeyed structure–activity relationships (SARs) set forth in the 1970’s. From these studies, carboplatin and oxaliplatin were identified and both have gone on to receive worldwide approval as chemotherapeutics. Today they continue to play an important role in cancer treatment and management. However, the prevalence of inherent and acquired resistance to platinum treatment requires the development of new complexes that operate through different mechanisms. Monofunctional DNA binding platinum(II) complexes represent an alternative class of very potent anticancer agents. Unlike cisplatin and its derivatives, these complexes contain only one labile ligand and thus form a single covalent bond to DNA. Pyriplatin, cis-diammine(pyridine)chloroplatinum(II), a monofunctional, cationic platinum(II) compound, displayed a spectrum of activity that differed from that of any of the clinically approved platinum drugs. However, the overall potency of pyriplatin was much less than that of cisplatin in all cancer cell lines tested. We subsequently pursued a rational, structure-based approach for improving the potency of monofunctional platinum anticancer drugs. By determining the X-ray structure of RNA polymerase II stalled at a pyriplatin adduct on a short DNA duplex containing a bound RNA, they deduced that the pyriplatin–DNA adduct may prevent translocation from the active site and block RNA chain elongation because of steric interactions and hydrogen bonding with the bridge helix, operating by a mechanism of transcription inhibition that differs from that of cis-DDP. Based on this rationale, phenanthriplatin was designed, synthesized, and discovered to have significantly greater efficacy for killing cancer cells than either cis-DDP or oxaliplatin. Moreover, according to the National Cancer Institute (NCI) cytotoxicity screening assay, phenanthriplatin exhibited a unique cancer cell-killing profile compared to all other platinum agents held in the NCI archives. The success of phenanthriplatin strongly encouraged further study of monofunctional Pt(II) compounds as anticancer drug candidates. In the present work, we report the synthesis and anticancer activity of a trans-DDP based monofunctional phenanthridine Pt(II) complex, in further pursuit of novel platinum cancer drug candidates. trans-[Pt(NH3)2(phenanthridine)Cl]NO3, 1, was prepared following the procedure developed for phenanthriplatin and characterized by a variety of analytical methods, including H and C NMR spectroscopy, ESI-MS, and elemental analysis. Complex 1 was also structurally investigated by single crystal X-ray diffraction. The solid-state study reveals that complex 1 adopts the expected square-planar coordination geometry with a trans arrangement of ammine ligands. Similar to phenanthriplatin, the plane of the phenanthridine ligand in complex 1 is approximately perpendicular to that of the platinum coordination plane (Fig. 1). The distance between the platinum atom and the overhanging carbon atom on the phenanthridine ligand (black arrow in Fig. 1) of complex 1 (3.224 Å) is nearly identical to that previously reported for phenanthriplatin (3.220 Å). This observation suggests that, like phenanthriplatin, complex 1 may show selectivity for nucleophilic binding sites on DNA while inhibiting unfavourable reactions with cytoplasmic sulphur nucleophiles. We anticipated that, as with phenanthriplatin, the steric bulk of the phenanthridine ligand in complex 1 would impede procession of both DNA and RNA polymerases and may a Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. E-mail: lippard@mit.edu b The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Tehcnology, Cambridge, MA 02139, USA. E-mail: ohyilmaz@mit.edu † Electronic supplementary information (ESI) available. CCDC 1582154. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/ c8cc00393a ‡ Current address: School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. Received 17th January 2018, Accepted 14th February 2018

Initially the development of platinum-based anticancer agents was focused on the synthesis and evaluation of complexes that obeyed structure-activity relationships (SARs) 1 set forth in the 1970's. From these studies, carboplatin and oxaliplatin were identified and both have gone on to receive worldwide approval as chemotherapeutics. Today they continue to play an important role in cancer treatment and management. However, the prevalence of inherent and acquired resistance to platinum treatment [2][3][4] requires the development of new complexes that operate through different mechanisms. Monofunctional DNA binding platinum(II) complexes represent an alternative class of very potent anticancer agents. 5 Unlike cisplatin and its derivatives, these complexes contain only one labile ligand and thus form a single covalent bond to DNA.
Pyriplatin, cis-diammine(pyridine)chloroplatinum(II), a monofunctional, cationic platinum(II) compound, displayed a spectrum of activity that differed from that of any of the clinically approved platinum drugs. 6 However, the overall potency of pyriplatin was much less than that of cisplatin in all cancer cell lines tested. We subsequently pursued a rational, structure-based approach for improving the potency of monofunctional platinum anticancer drugs. 7 By determining the X-ray structure of RNA polymerase II stalled at a pyriplatin adduct on a short DNA duplex containing a bound RNA, they deduced that the pyriplatin-DNA adduct may prevent translocation from the active site and block RNA chain elongation because of steric interactions and hydrogen bonding with the bridge helix, operating by a mechanism of transcription inhibition that differs from that of cis-DDP. Based on this rationale, phenanthriplatin was designed, synthesized, and discovered to have significantly greater efficacy for killing cancer cells than either cis-DDP or oxaliplatin. 6 Moreover, according to the National Cancer Institute (NCI) cytotoxicity screening assay, phenanthriplatin exhibited a unique cancer cell-killing profile compared to all other platinum agents held in the NCI archives. The success of phenanthriplatin strongly encouraged further study of monofunctional Pt(II) compounds as anticancer drug candidates. In the present work, we report the synthesis and anticancer activity of a trans-DDP based monofunctional phenanthridine Pt(II) complex, in further pursuit of novel platinum cancer drug candidates.
trans-[Pt(NH 3 ) 2 (phenanthridine)Cl]NO 3 , 1, was prepared following the procedure developed for phenanthriplatin 6 and characterized by a variety of analytical methods, including 1 H and 13 C NMR spectroscopy, ESI-MS, and elemental analysis. Complex 1 was also structurally investigated by single crystal X-ray diffraction.
The solid-state study reveals that complex 1 adopts the expected square-planar coordination geometry with a trans arrangement of ammine ligands. Similar to phenanthriplatin, the plane of the phenanthridine ligand in complex 1 is approximately perpendicular to that of the platinum coordination plane (Fig. 1). The distance between the platinum atom and the overhanging carbon atom on the phenanthridine ligand (black arrow in Fig. 1) of complex 1 (3.224 Å) is nearly identical to that previously reported for phenanthriplatin (3.220 Å). This observation suggests that, like phenanthriplatin, complex 1 may show selectivity for nucleophilic binding sites on DNA while inhibiting unfavourable reactions with cytoplasmic sulphur nucleophiles.
We anticipated that, as with phenanthriplatin, the steric bulk of the phenanthridine ligand in complex 1 would impede procession of both DNA 8  interfere with other essential enzymatic functions, 10 leading to potent anticancer activity. The in vitro anticancer activity of complex 1 was initially probed using fluorescence microscopy and the LIVE/DEAD cell assay, which is a combination of the ethidium homodimer-1 assay and staining with calcein AM (Fig. 2). Live cells stained with calcein AM yield a green fluorescence signal, whereas dead cells exhibit no fluorescence or a red signal due to the ethidium homodimer-1. As observed in Fig. 2, A2780 ovarian cancer cells treated with complex 1 (10 mM) for 48 h were mostly dead, whereas control cells without any drug treatment under the same conditions were alive. The IC 50 for complex 1 against A2780 at 48 h is 4.94 AE 1.52 mM.
The cytotoxicity of the newly synthesized monofunctional complex 1 was next evaluated against a panel of four human cancer cell lines of different origin (A2780, ovarian cancer; A2780CP70, cisplatin-resistant ovarian cancer; MCF-7, breast cancer; HCT116, colorectal cancer) using the MTT [3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Table 1). Cell viability was recorded after 72 h and compared to results obtained with cisplatin, oxaliplatin, and phenanthriplatin under identical conditions. Complex 1 displayed cytotoxicity similar to that of oxaliplatin but failed to perform as well as phenanthriplatin.
The selectivity of complex 1 for tumour cells over healthy cells was probed next. Normal lung fibroblasts (MRC5) and cancerous lung (A549) cells were used to evaluate the selectivity of complex 1 for cancer vs. healthy cells. Based on the MTT assay, the ratio of IC 50 values in healthy MRC5 cells to those in cancerous A549 cells was 0.9 for cisplatin compared with 2.9 for complex 1. The higher ratio obtained for complex 1 reveals its selectivity for cancer cells, at least in the cellular monolayer assays used in this study.
Next, we sought to quantitate the cellular uptake and the subcellular distribution of complex 1. The nuclei, cytoplasm, membrane, and whole cell concentrations of platinum were measured by graphite furnace atomic absorption spectroscopy (GFAAS) after treatment of the cells with the compounds for 5 h (Fig. 3). Phenanthriplatin was used as a comparison, and oxaliplatin and cisplatin were used as controls. After 5 h incubation with the Pt compounds, phenanthriplatin and complex 1 were taken up by cells more effectively than cisplatin or oxaliplatin. These results reflect the ability of the larger, hydrophobic heterocyclic phenanthridine ligand to facilitate uptake of the phenanthriplatin and complex 1 cations through the cytoplasmic membrane. However, the uptake of phenanthriplatin is, surprisingly, much higher than that of complex 1, indicating that geometric isomerism may play an important role in cellular uptake. Most of the platinum is found in the cytoplasm. The higher cellular uptake of phenanthriplatin most likely contributes to its enhanced cytotoxicity compared with the other platinum compounds.
DNA is the cellular target of platinum-based anticancer agents, thus the ability of complex 1 to platinate nuclear DNA was assessed. Two million HCT116 cells were treated with growth medium containing 20 mM cisplatin, oxaliplatin, phenanthriplatin, or complex 1 for 5 h, followed by incubation in fresh media for an additional 16 h. The intracellular DNA was isolated and the quantity of bound platinum was measured by GFAAS. The extent of DNA platination was determined to be 23.2 AE 3.6 Pt adducts per 10 6 nucleotides for cisplatin, 6.7 AE 1.0 Pt adducts per 10 6 nucleotides for oxaliplatin, 87.8 AE 6.6 Pt adducts per 10 6 Fig. 1 Comparison of the X-ray crystal structures of phenanthriplatin (left) and 1 (right). The platinum-carbon distances shown reveal that these two complexes exhibit similar degrees of steric protection from incoming nucleophiles at the axial positions.   nucleotides for phenanthriplatin, and 6.3 AE 1.6 Pt adducts per 10 6 nucleotides for complex 1.
The effect of cisplatin, oxaliplatin, phenanthriplatin, and complex 1 on supercoiled pUC19 DNA in Tris-HCl buffer (0.1 M, pH 7.4) at 37 1C after incubating in the dark for 6 h was studied by agarose gel electrophoresis (Fig. 4). Comparing the migration of pUC19 in the presence of the different platinum agents we see that DNA modified by complex 1 behaves similarly to that modified by cisplatin and oxaliplatin. In contrast, migration of phenanthriplatin 11 treated DNA is consistent with relaxation of the negatively supercoiled plasmid in response to intercalation of, and/or photocleavage by, the phenanthridine ring. 12,13 Our results therefore indicate that both the choice of coordinating ligands as well as the stereochemistry at the platinum center dictate the ability of platinum compounds to unwind duplex DNA. 14 gH2AX, the phosphorylated form of histone protein H2AX, is a known biomarker of DNA damage induced by platinum drugs. 15,16 gH2AX can be detected by immunostaining. As shown in Fig. 5, fluorescence microscopy was used to visualize the localization of gH2AX in the nucleus of HCT116 cells treated with complex 1, indicating genomic DNA damage. Apoptotic cells normally exhibit changes in cell morphology, such as blebbing, chromatin condensation, and nuclear fragmentation, which can be observed by fluorescence microscopy. As shown in Fig. 5, morphological changes occur following treatment of HCT116 cells with 20 mM complex 1 for 24 h. Chromatin condensation and nuclear fragmentation were also identified by Hoechst staining. All these cell-based experiments clearly indicate that complex 1 can effectively induce DNA damage and thus lead to cell cycle arrest and apoptosis in cancer cells.
Finally, intestinal organoids are three-dimensional primary cultures derived from intestinal stem cells that closely recapitulate the in vivo tissue structure. 17 As previously described, organoid cellular composition closely mimics in vivo biology in terms of secretory function, absorptive function, and pathophysiologic states. 18 Organoid assays have therefore gained in popularity for assessing drug toxicity and selectivity in vivo. Furthermore, organoid cultures are amenable for high-throughput drug   screening assays that can accurately predict in vivo outcomes. 19 The adenomatous polyposis coli (APC) gene product is essential for intestinal stem cell homeostasis as it regulates intracellular beta-catenin concentration and subsequent stem cell proliferation. Loss of function mutations in the APC gene occur as early events in a majority of colorectal cancers. 20 Additional oncogenic mutations such as those involving KRAS and TP53 subsequently occur and are associated with more aggressive tumor phenotypes, permitting local invasion and metastasis to lymph nodes and the liver.
To validate the efficacy of phenanthriplatin and complex 1, we utilized the Apc-knockout Kras G12D p53-knockout (AKP) engineered mouse intestinal cancer organoid model. By quantifying organoid formation, morphology, and cell viability, we determined the cytotoxicity of each drug (Fig. 6). Phenanthriplatin and complex 1 both exhibited significantly lower IC 50 values than that of both oxaliplatin and cisplatin. Taken together, these findings indicate that complex 1 has enhanced potency in treating intestinal cancers compared to oxaliplatin. In vivo studies are needed in order to understand how these results reflect the potential use of complex 1 clinically.
In conclusion, by replacing one of the chloride ligands of trans-DDP with the phenanthridine ligand, the monofunctional Pt(II) complex 1 was successfully synthesized, and it functioned as an effective anticancer agent. The results of cellular uptake, DNA platination, and DNA unwinding studies show very different activities between phenanthriplatin and its geometric isomer complex 1. This result indicates that the stereochemistry at the Pt(II) center may play a great role in affecting the properties of monofunctional platinum based anticancer agents.

Conflicts of interest
There are no conflicts to declare.