In the sentence extracted from biomedical literature, identify all the disease entities. The required answer format is the same sentence with HTML <span> tags to mark up specific entities. 
### Entity Markup Guides:
Use <span class="disease"> to denote a disease.

### Input Text: Biochemical fractionation of brain and liver tissues showed that emerin was present in nuclei purified by centrifugation through 65 % sucrose and was absent from soluble fractions ( post - 100 , 000 g ) .
### Output Text:
Biochemical fractionation of brain and liver tissues showed that emerin was present in nuclei purified by centrifugation through 65 % sucrose and was absent from soluble fractions ( post - 100 , 000 g ) . 