In the sentence extracted from biomedical literature, identify all the disease entities. The required answer format is the same sentence with HTML <span> tags to mark up specific entities. 
### Entity Markup Guides:
Use <span class="disease"> to denote a disease.

### Input Text: By using this approach , we were able to identify mutation in 22 new patients , but the overall efficiency of the procedure when we used a single - pass regimen was only 48 % .
### Output Text:
By using this approach , we were able to identify mutation in 22 new patients , but the overall efficiency of the procedure when we used a single - pass regimen was only 48 % . 