In the sentence extracted from biomedical literature, identify all the disease entities. The required answer format is the same sentence with HTML <span> tags to mark up specific entities. 
### Entity Markup Guides:
Use <span class="disease"> to denote a disease.

### Input Text: A large fragment of emerin cDNA was prepared by PCR and expressed as a recombinant protein in Escherichia coli .
### Output Text:
A large fragment of emerin cDNA was prepared by PCR and expressed as a recombinant protein in Escherichia coli . 