Endo- and exocytosis can be analyzed using the ratiometric and superecliptic pH-sensitive types of this protein called pHluorin (FLOOR-in).
Endo- and exocytosis can be analyzed using the ratiometric and superecliptic pH-sensitive types of this protein called pHluorin (FLOOR-in). A biosensor for the glutathione redox potential and thus ROS production is the "Mito-Ro" mutant of this protein. Some mutants of this protein can't be deprotonated at a specific site because the HBI tripeptide that is normally ionized after cyclization has its phenol replaced with an indole. That mutation in this protein has a substitution for tryptophan at tyrosine-66. This protein was discovered at Friday Harbor labs by Shimomura Osamu, who shared a Nobel Prize with Chalfie and Tsien for their research on this beta-barrel protein. For 10 points, name this fluorophore with a major emission peak at 509 nanometers derived from the bioluminescent Aequorea jellyfish.
Endo- and exocytosis can be analyzed using the ratiometric and superecliptic pH-sensitive types of this protein called pHluorin (FLOOR-in).
GFP [or green fluorescent protein]