The artificial production of these proteins requires the use of EBV-immortalized cell lines sensitive to aminopterin (uh-MEEN-oh-tare-in).
The artificial production of these proteins requires the use of EBV-immortalized cell lines sensitive to aminopterin (uh-MEEN-oh-tare-in). One kind of these proteins is produced by combining spleen cells and myeloma cells from mice. These proteins have additional diversity generated through the addition of N and P nucleotides during (*) VDJ recombination, and can undergo rounds of somatic mutation to increase their affinity. In Western blots, pairs of them are used to recognize proteins of interest. Plasma cells secrete these proteins into the serum, and they occur in five different isotypes that contain heavy and light chains. For 10 points, name these Y-shaped proteins expressed by B-cells that bind to antigens, which include IgG.
The artificial production of these proteins requires the use of EBV-immortalized cell lines sensitive to aminopterin (uh-MEEN-oh-tare-in).
monoclonal antibodies [or immunoglobulin until "IgG" is mentioned]