Isoschizomers of these molecules act on identical substrates.
Isoschizomers of these molecules act on identical substrates. The presence of excess glycerol can cause these molecules to exhibit star activity. Polylinkers, also called multiple cloning sites, are designed to be recognized by multiple examples of these molecules. Some protocols for site-directed mutagenesis use one of these enzymes from the bacterium Diplococcus pneumoniae to destroy the original methylated template. A method of paternity testing compares the lengths of fragments produced by these enzymes in RFLP analysis. These enzymes cleave the phosphodiester bonds of their substrate to produce blunt and sticky ends, such as the ones produced by EcoRI ["ecoR-one"]. For 10 points, name these enzymes which recognize and cut specific sequences in DNA.
Isoschizomers of these molecules act on identical substrates.
restriction enzymes [or restriction endonucleases; prompt on "nucleases"]