Reeves et al. isolated a ";pyrophosphate-dependent"; form of this enzyme found in the cytosol of some plant cells.
Reeves et al. isolated a ";pyrophosphate-dependent"; form of this enzyme found in the cytosol of some plant cells. Whether a positive or negative effector binds to this enzyme changes whether or not its Arg-162 or Glu-161 residue faces the active site. A mutation in this enzyme's gene leads to a rare glycogen storage disease called GSD7 or Tarui disease. A second isoform ofthis enzyme forms a homodimer with FBPase-2 and is responsible for creating an activator of the first isoform of this enzyme. It's not hemoglobin, but phospho-enol-pyruvate and citrate can bind to this tetrameric enzyme, causing it to shift from the active R state to the inactive T state. In a classic example of feedback inhibition, this strongly exergonic enzyme is allosterically activated by AMP and inhibited by ATP, causing cells to only produce ATP when they need it. For 10 points, name this important regulatory enzyme in glycolysis which catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate.
Reeves et al. isolated a ";pyrophosphate-dependent"; form of this enzyme found in the cytosol of some plant cells.
phosphofructokinase-1 [or PFK-1]