One variant of this assay developed by Czerkinsky is used to find cells that release particular cytokinesby spotting cells onto a membrane.
One variant of this assay developed by Czerkinsky is used to find cells that release particular cytokinesby spotting cells onto a membrane. Plates used for this procedure can be used to pan phage displaylibraries. Like a western blot, this assay can use bovine serum albumin as a blocking agent. The results ofthis assay are often reported as an index value with a pre-set cutoff. This assay can be made quantitative if acheckerboard titration is used beforehand. Variations of this assay include one in which antigen is added toan adsorbed antibody and another in which serum is added on top of already-adsorbed antigen; those arethe sandwich and indirect forms. This assay uses a microtiter plate. For 10 points, name this assay used tofind serum antibody concentrations or the presence of a particular antigen, commonly known by afive-letter acronym.
One variant of this assay developed by Czerkinsky is used to find cells that release particular cytokinesby spotting cells onto a membrane.
ELISA [or Enzyme-linked Immunosorbent Assay; or ELISPOT]