A "gap" in the readout of this procedure is used to find binding sites in the nuclease footprint assay, while a two-dimensional version uses isoelectric focusing in its second half.  
A "gap" in the readout of this procedure is used to find binding sites in the nuclease footprint assay, while a two-dimensional version uses isoelectric focusing in its second half.  The SDS-PAGE variety is performed before the transfer step of a western blot protocol, and it is commonly run in a Tris- acetate buffer.  The namesake matrix usually contains an intercalating agent like ethidium bromide, and the lane on the end is sometimes reserved for a ladder, which is a solution of fragments with known size.  FTP, what is this lab technique commonly done using acrylamide or agarose, that applies an electric field to protein or DNA fragments in order to separate them by size?
A "gap" in the readout of this procedure is used to find binding sites in the nuclease footprint assay, while a two-dimensional version uses isoelectric focusing in its second half.  
{Gel Electrophoresis} or {Running a Gel} or {Gellin’}