This procedure allows us to determine the number of distinct polypeptides in a protein.
This procedure allows us to determine the number of distinct polypeptides in a protein. Dansyl chloride and dabsyl chloride yield more detectable derivatives than 1-fluoro-2,4-dinitrobenzene, or FDNB, which loses a fluorine in attaching to the amino-terminus. After the protein is hydrolyzed, the FDNB- labeled N-terminus residue is identified, giving us phenylalanine and glycine for the two chains of insulin. Improved upon by Edman degradation, FTP name this chemical procedure, namesake of a two-time British Nobel prize winner who used it to sequence insulin.
This procedure allows us to determine the number of distinct polypeptides in a protein.
{Sanger} degradation or sequencing