Using Ac-DAP to facilitate "de-blocking" in this reaction eliminates the need for an extra purifiÂcation step.
Using Ac-DAP to facilitate "de-blocking" in this reaction eliminates the need for an extra purifiÂcation step. Wang et al developeda "ladder" technique that alternates cycles of this reaction with mass spectrometry. Dansyl chloride is sometimes usedas a reagent in this reaction because it produces flfluorescent sulfonamides, particularly in the "subtractive" type. This reaction improves upon an earlier technique that used a benzyl alcohol to start a Curtius rearrangement. Humpback whale myoglobinwas used as a reagent in the Âfirst automation of this reaction, developed by Begg and its namesake. Following an extractioninto an organic solvent and an exposure to acidic conditions, a phenylthiohydantoin derivative is produced in this reaction,an improvement over a similar procedure named for Max Bergmann, which begins with the reaction of phenylisothiocyanatewith an uncharged terminal amino group. For 10 points, name this reaction invented by a Swedish chemist, which can synthesizeup to 30 amino acids in a peptide.
Using Ac-DAP to facilitate "de-blocking" in this reaction eliminates the need for an extra purifiÂcation step.
Edman degradation [prompt on "degradation"]