In a Far Eastern blot, the result of this procedure is transferred to a PVDF membrane.
In a Far Eastern blot, the result of this procedure is transferred to a PVDF membrane. Advancements in this method include the automation of droplet delivery and stationary phases impregnated with UV indicator dyes. General use developing agents include ceric ammonium molybdate and p-anisaldehyde, while ninhydrin is often used for amino acids. The key value in this technique is the retardation factor R-sub-f, which is the ratio of the migration distance of the analyte over the migration distance of the solvent front. As with a similar method performed with paper, the solvent moves through the stationary phase by capillary action. For 10 points, name this analytical separation technique often carried out on a silica-covered plate.
In a Far Eastern blot, the result of this procedure is transferred to a PVDF membrane.
thin-layer chromatography [or TLC; prompt on partial answer]