Bad results in this technique can occur due to the "smiling" effect.
Bad results in this technique can occur due to the "smiling" effect. Tris, EDTA, and either borate or acetate make up the most common buffer used in it. Mobility shift assays to study protein-DNA interactions use this technique. This procedure is the final step of Sanger sequencing, after chain-termination occurs from a ddNTP. The intercalating agent (*) ethidium bromide is frequently used in this technique. The product of this procedure is blotted onto a nitrocellulose membrane and treated with radiolabelled probes in a technique named for Southern. In this technique, a comb is inserted into an agarose or polyacrylamide matrix to form wells. For 10 points, name this technique in which an electromotive force separates DNA fragments based on their length.
Bad results in this technique can occur due to the "smiling" effect.
gel electrophoresis