One assay used to measure its activity involves the introduction of FdG via hypotonic shock, and the accumulation of the product of that hydrolysis reaction, fluorescin, is a marker for the activity of this enzyme. The combined deficiency of neuramin
One assay used to measure its activity involves the introduction of FdG via hypotonic shock, and the accumulation of the product of that hydrolysis reaction, fluorescin, is a marker for the activity of this enzyme. The combined deficiency of neuraminidase and this enzyme occurs due to the lack of a glycoprotein which prevents the proteolytic degradation of this enzyme, and is called the Goldberg syndrome. This enzyme also carries out an isomerization to produce a product which, like IPTG, acts as in inducer for this enzyme by inactivating its repressor, whereas PETG is a reversible inhibitor for it. It is the product of a polycistronic mRNA which also codes for a ?permease and a transacetylase, and its activity can be probed by a colorimetric assay wherein a dark-blue product is formed from ONPG or X-Gal. For 10 points, identify this protein synthesized by the lac operon, which helps digest a namesake monosaccharide.
One assay used to measure its activity involves the introduction of FdG via hypotonic shock, and the accumulation of the product of that hydrolysis reaction, fluorescin, is a marker for the activity of this enzyme.
{beta galactosidase}