Rong and Golic used a linearized P-element construct and a heat-sensitive FLP system to adapt this process to Drosophila. A common version of this process uses gancyclovir or G418 as a counter-selection agent since the construct contains the HSV-tk gene, while positive selection is achieved using a neomycin resistance gene. This process usually begins with a transfection in an embryonic stem-cell line with the goal of creating an organism with an altered germline, and the "hit and run" strategy 
Rong and Golic used a linearized P-element construct and a heat-sensitive FLP system to adapt this process to Drosophila. A common version of this process uses gancyclovir or G418 as a counter-selection agent since the construct contains the HSV-tk gene, while positive selection is achieved using a neomycin resistance gene. This process usually begins with a transfection in an embryonic stem-cell line with the goal of creating an organism with an altered germline, and the "hit and run" strategy can perform replacement as well as this process. Capecchi, Evans, and Smithies won the 2007 Nobel Prize for developing this process, which also can be used to add transgenic constructs into a particular locus. For 10 points, name this process commonly used to remove specific genes using homologous recombination, often used to create a namesake type of mouse.
Rong and Golic used a linearized P-element construct and a heat-sensitive FLP system to adapt this process to Drosophila.
Gene knockout [Accept reasonable equivalents; accept homologous recombination or gene targeting; accept gene knockin until "remove"; do not accept gene trapping; do not accept gene knockdown as that doesn't actually delete the gene]