One assay used to measure its activity involves the introduction of FdG via hypotonic shock, and the accumulation of the product of that hydrolysis reaction, fluorescin, is a marker for the activity o
One assay used to measure its activity involves the introduction of FdG via hypotonic shock, and the accumulation of the product of that hydrolysis reaction, fluorescin, is a marker for the activity of this enzyme. The combined deficiency of neuraminidase and this enzyme occurs due to the lack of a glycoprotein which prevents the proteolytic degradation of this enzyme, and is called the Goldberg syndrome. This enzyme also carries out an isomerization to produce a product which, like IPTG, acts as in inducer for this enzyme by inactivating its repressor, whereas PETG is a reversible inhibitor for it. It is the product of a polycistronic mRNA which also codes for a ?permease and a transacetylase, and its activity can be probed by a colorimetric assay wherein a dark-blue product is formed from ONPG or X-Gal. For 10 points, identify this protein synthesized by the lac operon, which helps digest a namesake monosaccharide.
One assay used to measure its activity involves the introduction of FdG via hypotonic shock, and the accumulation of the product of that hydrolysis reaction, fluorescin, is a marker for the activity of this enzyme.
{beta galactosidase}