One variety of this process can use SYBR green florescent dye to make it quantitative in real-time. Because Pfu has three-prime to five-prime proofreading ability, its use in this process results in increased accuracy over an enzyme that produces three-prime adenosine overhangs. Steps of this process include [*] denaturing, during which the template strands detach, annealing, during which primers attach, and elongation, during which thermostable 
One variety of this process can use SYBR green florescent dye to make it quantitative in real-time. Because Pfu has three-prime to five-prime proofreading ability, its use in this process results in increased accuracy over an enzyme that produces three-prime adenosine overhangs. Steps of this process include [*] denaturing, during which the template strands detach, annealing, during which primers attach, and elongation, during which thermostable Taq ["tack"] polymerase adds new nucleotides to synthesize a complementary strand. Invented by Kary Mullis, for 10 points, identify this process by which a strand of DNA is amplified.
One variety of this process can use SYBR green florescent dye to make it quantitative in real-time.
{polymerase chain} reaction [accept prefixation of “real time,” “quantitative,” “RT,” or “Q,” before mentioned]