Some organisms like Haemophilus and Legionella do not respond well to its last part, the usage of safranin, so sometimes a basic solution of fuchsin is substituted instead.  The decolorization arising
Some organisms like Haemophilus and Legionella do not respond well to its last part, the usage of safranin, so sometimes a basic solution of fuchsin is substituted instead.  The decolorization arising from what is generally a mixture of ethanol and acetone serves to get rid of a lipid layer, and its second step generally uses iodine as mordant to fix the dye.  Its first and most important step sometimes uses methylene blue instead of the usual crystal-violet dye. FTP, identify this test for the presence of peptidoglycan in bacterial cell walls, named for its Danish creator.
Some organisms like Haemophilus and Legionella do not respond well to its last part, the usage of safranin, so sometimes a basic solution of fuchsin is substituted instead.  
Gram stain