The fluorescent dye SYBR green I [S Y B R green one] and the "molecular beacon" method use fluorescence to measure its progress, and the RACE method is based on it. Elevated presence of magnesium ions increases its mutation rate, and it is often a precursor to single-strand conformation polymorphism. Its three steps consist of the heat-induced separation of two template strands, reduction of temperature so the primers can
The fluorescent dye SYBR green I [S Y B R green one] and the "molecular beacon" method use fluorescence to measure its progress, and the RACE method is based on it. Elevated presence of magnesium ions increases its mutation rate, and it is often a precursor to single-strand conformation polymorphism. Its three steps consist of the heat-induced separation of two template strands, reduction of temperature so the primers can anneal to the strands, and addition of nucleotides using the Taq polymerase enzyme. For 10 points, name this technique developed in 1983 by Kary Mullis, which is used for quickly copying segments of DNA.
The fluorescent dye SYBR green I [S Y B R green one] and the "molecular beacon" method use fluorescence to measure its progress, and the RACE method is based on it.