One method of creating these molecules involves culturing in an IL-6 rich medium or injecting into the peritoneal cavity of a mouse and collecting the ascites. The classical method of creating these molecules was developed by Cesar Milstein, and involves growing cells defici
One method of creating these molecules involves culturing in an IL-6 rich medium or injecting into the peritoneal cavity of a mouse and collecting the ascites. The classical method of creating these molecules was developed by Cesar Milstein, and involves growing cells deficient in HGPRT in a medium containing hypoxanthine, thymidine, and aminopterin. Examples of these molecules used therapeutically include adalimumab, which inhibits TNF-alpha, and Trastuzumab, or Herceptin, used to treat breast cancer. These proteins are created by fusing a single plasma cell with a mouse myeloma cell, creating a hybridoma that secretes only one heavy-light chain pair. For 10 points, name these pharmaceuticals that consist of an antibody targeting a single epitope.
One method of creating these molecules involves culturing in an IL-6 rich medium or injecting into the peritoneal cavity of a mouse and collecting the ascites.