One method of creating these molecules involves culturing in an IL-6 rich medium or injecting into the peritoneal cavity of a mouse and collecting the ascites. The classical method of creating these molecules was developed by Cesar Milstein, and invo
One method of creating these molecules involves culturing in an IL-6 rich medium or injecting into the peritoneal cavity of a mouse and collecting the ascites. The classical method of creating these molecules was developed by Cesar Milstein, and involves growing cells deficient in HGPRT in a medium containing hypoxanthine, thymidine, and aminopterin. Examples of these molecules used therapeutically include adalimumab, which inhibits TNF-alpha, and Trastuzumab, or Herceptin, used to treat breast cancer. These proteins are created by fusing a single plasma cell with a mouse myeloma cell, creating a hybridoma that secretes only one heavy-light chain pair. For 10 points, name these pharmaceuticals that consist of an antibody targeting a single epitope.
One method of creating these molecules involves culturing in an IL-6 rich medium or injecting into the peritoneal cavity of a mouse and collecting the ascites.