Enzymes of this type with Rev3 catalytic subunits partake in translesion synthesis. Xeroderma pigmentosum is caused by a mutation in the eta member of this enzyme family. PCNA is an example of a sliding clamp protein, which holds these enzymes in place to prevent them from falling off of their substrate. Subtilisin's cleavage of them yields the Klenow fragment, and they are used to incorporate chain-terminating ddNTPs in Sanger sequencing. They possess (*) proofreading capabilities and 3-prime to 5-prime exonuclease act
Enzymes of this type with Rev3 catalytic subunits partake in translesion synthesis. Xeroderma pigmentosum is caused by a mutation in the eta member of this enzyme family. PCNA is an example of a sliding clamp protein, which holds these enzymes in place to prevent them from falling off of their substrate. Subtilisin's cleavage of them yields the Klenow fragment, and they are used to incorporate chain-terminating ddNTPs in Sanger sequencing. They possess (*) proofreading capabilities and 3-prime to 5-prime exonuclease activity. Pfu and Taq are examples of them used in a common laboratory procedure. For 10 points, name these enzymes that add nucleotides to the 5-prime end of a growing DNA strand in replication, which can be used to amplify DNA in a namesake "chain reaction."
Enzymes of this type with Rev3 catalytic subunits partake in translesion synthesis.