One variety of this process uses reverse transcription to deduce gene expression levels based on var
One variety of this process uses reverse transcription to deduce gene expression levels based on varying amounts of mRNA present. One parameter in this process is dependent on the GC-content of primers used because of differences in stability, and magnesium chloride appears as part of the "master mix" due to its role as a co-factor for the primary enzyme used. Often using a chemical taken from the bacterium Thermus aquaticus, this technique uses a thermocycler to carry out the temperature-dependent steps of denaturaton, annealing, and elongation. For 10 points, name this biochemical technique used to rapidly copy DNA strands.
One variety of this process uses reverse transcription to deduce gene expression levels based on varying amounts of mRNA present.
Polymerase Chain Reaction [Be lenient and accept any types of PCR.]