The results of one form of this technique are commonly analyzed using Melanie or REDFIN. One reagent used in this technique is a sulfonated triphenylm
The results of one form of this technique are commonly analyzed using Melanie or REDFIN. One reagent used in this technique is a sulfonated triphenylmethane derivative that is also used in the Bradford assay, though increased sensitivity can be obtained using silver instead. Ammonium persulfate and TEMED are used as polymerizing agents to create the matrix for this technique, which commonly has a separating component underneath a stacking component. A two-dimensional variation of this technique uses a pH (*) gradient to first separate analytes by isoelectric point. This technique uses a Tris-glycine buffer system named for Laemelli. Native variations of this technique don't use anionic detergents, and the results of this technique are commonly visualized using Coomassie blue. Samples in this technique are commonly boiled in DTT or beta-mercaptoethanol. The results of this technique are sandwiched between two pieces of filter paper and a piece of PVDF membrane in the transfer step of a western blot. For 10 points, name this technique that uses a polyacrylamide matrix to separate proteins.
The results of one form of this technique are commonly analyzed using Melanie or REDFIN.
PAGE [or polyacrylamide gel electrophoresis, or protein gel electrophoresis; or protein electrophoresis accept more specific answers like SDS-PAGE, native PAGE, QNPC-PAGE, 2D SDS-PAGE, etc; need both protein and electrophoresis for points to distinguish from DNA gel electrophoresis; prompt on partial answers]