This technique is commonly used alongside Pierce's multiplex TMT tags for quantitation. This technique is used after combining the labelled and unlabeled samples together in a SILAC experiment.  John Yates and Jimmy Eng developed the standard software for analyzing the results of this technique using database cross-correlation; that software is SEQUEST. The use of this technique on a drop of blood from a heel prick has replaced the use of Guthrie's test in screening infants for inborn errors of metabolism.  The output of this technique creates b-type or y-type (*) ions. This is a common follow-up and confirmatory experiment if PMF fails to identify a sample. Cleavage in this technique is commonly accomplished using collision-induced dissociation, and this technique is commonly used after cutting a band out of an SDS-PAGE gel followed by trypsin digestion. This technique relies on one analyzer to select a single peptide, which is then fragmented and run through a second analyzer. For 10 points, name this technique from proteomics which essentially uses a pair of mass spectrometers in series.
This technique is commonly used alongside Pierce's multiplex TMT tags for quantitation. This technique is used after combining the labelled and unlabeled samples together in a SILAC experiment.  John Yates and Jimmy Eng developed the standard software for analyzing the results of this technique using database cross-correlation; that software is SEQUEST. The use of this technique on a drop of blood from a heel prick has replaced the use of Guthrie's test in screening infants for inborn errors of metabolism.  The output of this technique creates b-type or y-type (*) ions. This is a common follow-up and confirmatory experiment if PMF fails to identify a sample. Cleavage in this technique is commonly accomplished using collision-induced dissociation, and this technique is commonly used after cutting a band out of an SDS-PAGE gel followed by trypsin digestion. This technique relies on one analyzer to select a single peptide, which is then fragmented and run through a second analyzer. For 10 points, name this technique from proteomics which essentially uses a pair of mass spectrometers in series.
This technique is commonly used alongside Pierce's multiplex TMT tags for quantitation.
MS/MS or MS2 or Tandem Mass Spectrometry [prompt on “mass spec” or “protein mass spec” or “peptide mass spec” throughout; do NOT accept anything with the word “fingerprinting” in it]