One pioneer of this technique also recently developed an imaging technique that uses hydrogel to make tissue transparent, called CLARITY. One protein used in this technique was made bistable by mutating cysteine-128, while another has a E159T slash T159C double mutant to increase speed and gain. A series of double-floxed-inverted open-reading-frame-containing AAV vectors were developed by Deisseroth et al for use in this technique. Kreitzer et. al. used this technique to activate direct-pathway medium spiny cells to treat a mouse model of (*) Parkinson's disease. The proteins used in this technique are either algae-derived halorhodopsin or channelrhodopsin or fusion proteins consisting of a rhodopsin extracellular domain and an adrenergic G-protein-coupled receptor intracellular domain. This technique was demonstrated in a mouse that was compelled to lick when a laser was shone through a
One pioneer of this technique also recently developed an imaging technique that uses hydrogel to make tissue transparent, called CLARITY. One protein used in this technique was made bistable by mutating cysteine-128, while another has a E159T slash T159C double mutant to increase speed and gain. A series of double-floxed-inverted open-reading-frame-containing AAV vectors were developed by Deisseroth et al for use in this technique. Kreitzer et. al. used this technique to activate direct-pathway medium spiny cells to treat a mouse model of (*) Parkinson's disease. The proteins used in this technique are either algae-derived halorhodopsin or channelrhodopsin or fusion proteins consisting of a rhodopsin extracellular domain and an adrenergic G-protein-coupled receptor intracellular domain. This technique was demonstrated in a mouse that was compelled to lick when a laser was shone through a fiber optic cable running into its brain. For 10 points, name this technique in which light is used to control populations of cells.
One pioneer of this technique also recently developed an imaging technique that uses hydrogel to make tissue transparent, called CLARITY.
optogenetics