A reagent used in this assay can be activated using Aroclor-1254. An alternative to this assay uses the Big Blue transgenic mouse strain. OECD 471 is the standard for this test, which can be extended into a 384-well plate format in the fluctuation test. Before this test, a dose rangefinder using TA100 is performed, and this test is often performed with rfa mutants that are unable to express lipopolysaccharide. The S9 fraction, which is microsomal preparations of rat liver, must be added to this assay because they have (*) cytochrome P450 activity, which may be required to activate some analytes like benzopyrene. This assay works by using an auxotrophic strain which require histidine, with revertants corresponding to a positive test. For 10 points, identify this biological test for determin
A reagent used in this assay can be activated using Aroclor-1254. An alternative to this assay uses the Big Blue transgenic mouse strain. OECD 471 is the standard for this test, which can be extended into a 384-well plate format in the fluctuation test. Before this test, a dose rangefinder using TA100 is performed, and this test is often performed with rfa mutants that are unable to express lipopolysaccharide. The S9 fraction, which is microsomal preparations of rat liver, must be added to this assay because they have (*) cytochrome P450 activity, which may be required to activate some analytes like benzopyrene. This assay works by using an auxotrophic strain which require histidine, with revertants corresponding to a positive test. For 10 points, identify this biological test for determining whether a substance is carcinogenic.
A reagent used in this assay can be activated using Aroclor-1254.
Ames test