Although it is not catalytic RNA, T. A. Steitz proposed that these enzymes use a two-divalent-metal-ionmechanism for catalysis. A nuclease and one of these enzymes is used in the technique of nick translation.The first one of
Although it is not catalytic RNA, T. A. Steitz proposed that these enzymes use a two-divalent-metal-ionmechanism for catalysis. A nuclease and one of these enzymes is used in the technique of nick translation.The first one of these enzymes to be discovered can be cleaved by subtilisin to yield a Klenow fragment,which lacks 5-prime to 3-prime exonuclease activity. These enzymes add a deoxynucleotide to a freehydroxyl at the 3-prime end of a nucleic acid strand. Two of the most commonly used types of them are Pfuand Taq, which are central to a technique invented by Kary Mullis. For 10 points, identify these enzymethat are used to amplify DNA in a namesake "chain reaction."
Although it is not catalytic RNA, T. A. Steitz proposed that these enzymes use a two-divalent-metal-ionmechanism for catalysis.
DNA polymerases [or pols]