The impact of applying COT1-DNA to analytes of this technique was the subject of a 2009 paper by Trifonov et al., and one variant of this technique can be used to distinguish between individual strands by BrdU incorporation during replication. Another variant of this technique exploits the fact that PNA has higher affinity for DNA than natural oligonuclueotides, and SKY is an alternative to a multi-color variant of this technique. It's not PCR, but this technique has a quantitative variant that is often used to measure telomere lengths, and that variant is often performed in conjunction with flow cytometry. This technique is used to detect and map locations of genes along chromosomes by making probes that base pair 
The impact of applying COT1-DNA to analytes of this technique was the subject of a 2009 paper by Trifonov et al., and one variant of this technique can be used to distinguish between individual strands by BrdU incorporation during replication. Another variant of this technique exploits the fact that PNA has higher affinity for DNA than natural oligonuclueotides, and SKY is an alternative to a multi-color variant of this technique. It's not PCR, but this technique has a quantitative variant that is often used to measure telomere lengths, and that variant is often performed in conjunction with flow cytometry. This technique is used to detect and map locations of genes along chromosomes by making probes that base pair with the genes of interest. FTP, identify this technique, whose name refers to the fact that the sister chromatids being studied are held in place on a slide.
The impact of applying COT1-DNA to analytes of this technique was the subject of a 2009 paper by Trifonov et al., and one variant of this technique can be used to distinguish between individual strands by BrdU incorporation during replication.
FISH [or Fluorescent In Situ Hybridization; prompt on  In Situ Hybridization; accept specific types like quantitative FISH or Flow-FISH]