The MLPA procedure is used with multiple targets, and more widely used variants include the quantitative and real-time versions, which use fluorescent dyes.
Errors in this procedure can be corrected by replacing one of the reagents with PfuUltra or Pfx, while spurious products can be swamped out by ramping up the temperature in the "touchdown" variation. The MLPA procedure is used with multiple targets, and more widely used variants include the quantitative and real-time versions, which use fluorescent dyes. It begins with denaturation, which pries apart the template, followed by annealing, in which the primers attach. Taq polymerase then replicates the template, and the process repeats itself. FTP, name this technique invented by Kary Mullis that amplifies a segment of DNA.
Errors in this procedure can be corrected by replacing one of the reagents with PfuUltra or Pfx, while spurious products can be swamped out by ramping up the temperature in the "touchdown" variation.
{P}olymerase {C}hain {R}eaction