Used in HIV testing or when a western blot is insufficient, for 10 points, name this assay performed in a microtiter plate, which uses antibodies to quantify the amount of antigen in a sample.
When working with basement membranes, adding a chaotropic agent like NaSCN during this assay improves its fidelity, and Rheumatoid factor causes high levels of false positives in it. Reagents undergo a checkerboard titration before this assay, and Czerzinsky developed a version of it used to measure cytotoxic T-cell responses and identify cytokine-producing cells, known as its "SPOT" version. Another version is performed by first attaching one capture molecule to the substrate, known as the "sandwich" version. Often performed following a blocking step with BSA or casein and visualized using urease, alkaline phosphatase, or HRP, the more standard version of this assay requires the creation of a standard curve, known as the "indirect" version. Used in HIV testing or when a western blot is insufficient, for 10 points, name this assay performed in a microtiter plate, which uses antibodies to quantify the amount of antigen in a sample.
When working with basement membranes, adding a chaotropic agent like NaSCN during this assay improves its fidelity, and Rheumatoid factor causes high levels of false positives in it.
{ELISA} or {EIA} or {Enzyme}-linked {immuno}sorbent {assay}