For 10 points, name this procedure, in one form of which DNA flows through an agarose matrix due to the application of an electric field.
One form of this technique is usually performed in a tris-glycine solution, and another variant of this technique relies on the fact that a protein will come to rest a point at which its pI is equal to the surrounding pH. Proteins are often treated with mercaptoethanol prior to being subjected to one form of this technique in polyacrylamide, and ethydium bromide is often used to view the results of this technique. PCR products are run through this technique to scan for contamination, and a crucial step in performing this technique involves ensuring that the contents of the pipet will fall through the buffer into the desired well. For 10 points, name this procedure, in one form of which DNA flows through an agarose matrix due to the application of an electric field.
One form of this technique is usually performed in a tris-glycine solution, and another variant of this technique relies on the fact that a protein will come to rest a point at which its pI is equal to the surrounding pH. Proteins are often treated with mercaptoethanol prior to being subjected to one form of this technique in polyacrylamide, and ethydium bromide is often used to view the results of this technique.
gel electrophoresis [prompt on electrophoresis; accept specific types like DNA gel electrophoresis SDS-PAGE or isoelectric focusing]