That polymerase extends DNA primers in this process, which was first developed by Kary Mullis.
One variety of this process reduces side products by starting at a high temperature and then gradually lowering it, called the "touchdown" method. Fidelity in this process can be improved by using Pfx or Pfu, and its helicase-dependent type is carried out at constant rather than cycling temperature. Divalent cations and dNTPs are also used in this process, which commonly uses Taq, a type of heat-resistant polymerase. That polymerase extends DNA primers in this process, which was first developed by Kary Mullis. For 10 points, name this experimental technique developed to amplify genetic material outside of the cell.
One variety of this process reduces side products by starting at a high temperature and then gradually lowering it, called the "touchdown" method.
{p}olymerase {c}hain {r}eaction