This can then be implanted into a bacterial cell, which will then produce copies of the DNA molecule.
The use of caesium chloride gradients in the presence of ethidium bromide, though time consuming, allows for the purification of the supercoiled variety of these. They come in two classes: one that determines the production of colicins and the other that confers a resistance to antibiotics. The two types are called Col and R Factors. Through the use of endonucleases, they can be "cut" and have a foreign DNA molecule spliced in. This can then be implanted into a bacterial cell, which will then produce copies of the DNA molecule. FTP name these circular DNA molecules that replicate independently of the bacterial chromosome.
The use of caesium chloride gradients in the presence of ethidium bromide, though time consuming, allows for the purification of the supercoiled variety of these.
{Plasmid}