Band broadening in this procedure is equal to the sum of terms related to the eddy diffusion, lateral diffusion, and resistance to transfer, a result known as Van Deemter's equation.
Polyhistidine tagged proteins are purified using divalent cations in a variation of this technique. Band broadening in this procedure is equal to the sum of terms related to the eddy diffusion, lateral diffusion, and resistance to transfer, a result known as Van Deemter's equation. It can be sped up by pumping nitrogen gas through the apparatus, a variation called "flash" (*), and sepharose is used in the size exclusion variant of this procedure. DEAE or CM is used to separate particles by charge in the ion-exchange type. FTP, name this laboratory technique that separates an eluent by running it through a glass tube containing a stationary phase.
Polyhistidine tagged proteins are purified using divalent cations in a variation of this technique.
{Column Chromatography} (accept {Affinity chromatography} until *, prompt on {chromatography})