TMS is used as a chemical shift reference for this technique.
The accuracy of this technique can be improved by inserting a step for solid-phase extraction between HPLC and it in an example of a hyphenated technique. If samples are very dilute, as is often the case with proteins, tubes manufactured by Shigemi can be used for this process. For this process, the equation A plus B cosine theta plus C cosine two theta, where theta is the dihedral angle, can be used to find the J-coupling constant. Pascal's triangle is followed by the intensity ratios of multiplets in this technique. Samples for this process are often dissolved in DMSO or deuterated chloroform. TMS is used as a chemical shift reference for this technique. For 10 points, name this kind of spectroscopy that comes in proton and carbon-13 varieties.
The accuracy of this technique can be improved by inserting a step for solid-phase extraction between HPLC and it in an example of a hyphenated technique.
NMR spectroscopy [or nuclear magnetic resonance spectroscopy]