A reaction catalyzed by this enzyme is assayed by the deltaÂ–deltaÂ–C method.
This protein has a PHP domain in prokaryotes joined to its normal " nger," "thumb," and "palm" domains. A reaction catalyzed by this enzyme is assayed by the deltaÂ–deltaÂ–C method. NEB markets the Q5 form of this protein, which is used in Gibson assemblies as a fusion protein called Phusion, with a "P-H." Arthur Kornberg won a Nobel Prize for discovering this protein. PCNA clamps this enzyme to its substrate to enhance processivity. In the lab, this protein is mixed with magnesium chloride and dÂ·NÂ·TÂ·Ps and then thermally cycled between 72 degrees Celsius, 98 degrees Celsius, and the primer annealing temperature. Thermus aquaticus is the source of a heat-stable form of, for 10 points, what protein that elongates Okazaki fragments and ampli es DNA during a namesake "chain reaction"?
This protein has a PHP domain in prokaryotes joined to its normal " nger," "thumb," and "palm" domains.
DNA polymerase I [or DNA polymerase II; or any other number or Greek letter a0er it; or Taq polymerase; accept polymerase a0er "DNA"; prompt on "polymerase" before "DNA"]