The transcription factor used in this technique is also used with its upstream activation sequence to induce targeted gene expression in Drosophila.
A modi㻈cation of this technique is based on the activation of Ras ("rahs") when hSos ("h-sauce") is localized to the cell membrane. Three-amino-one-two-four-triazole, or 3-AT, can be used to evaluate expression of the HIS3 ("hiss-three") reporter in this method. The transcription factor used in this technique is also used with its upstream activation sequence to induce targeted gene expression in Drosophila. A form of this technique applicable in mammals involves the phosphorylation of gÂ·pÂ·130 by TÂ·YÂ·KÂ·2 and is known as the Kinase Substrate Sensor, or KISS. It can be extended to integral membrane proteins by using "Cub" and "Nub" protein domains in the split-ubiquitin system. It involves expression of a reporter gene when the activation domain and DNA-binding domain of transcription factor Gal4 ("gal" "four") are brought together by bait and prey proteins. For 10 points, name this technique that involves using S. cerevisiae to identify protein-protein interactions.
A modi㻈cation of this technique is based on the activation of Ras ("rahs") when hSos ("h-sauce") is localized to the cell membrane.
yeast 2-hybrid assay [or Y2H]