Lithium perÂ·chlorate and guanidinium thiocyanate (gwah-NID-ee-um "thigh"-
This process occurs in two steps in the LumryÂ–Eyring model. Thermal shi0 assays give the midpoint temperature for this process. It causes the ellipticity to go to zero in a CD experiment. This process can be likened to escaping from the bottom of the energy funnel into another local minimum, such as the molten globule. Lithium perÂ·chlorate and guanidinium thiocyanate (gwah-NID-ee-um "thigh"-oh-"SIGH"-an-ate) induce this process because they are chaotropes (KAY-oh-"tropes") on the Hofmeister series. SDS is added before PAGE ("page") to charge the sample and induce this process. A mix of 8-molar urea and beta-mercaptoethanol accomplished this destructive task in An nsen's experiment. Reducing disul de bonds and disrupting tertiary structure to make a random coil accomplishes, for 10 points, what process in which proteins lose their 3D structure?
This process occurs in two steps in the LumryÂ–Eyring model.
protein denaturation [or word forms; or protein unfolding or forms of the word unfold; or protein melting or forms of the word melt; prompt on protein "aggregation" or "protein linearization"; do not accept or prompt on "folding"]