The most common titrant in a Karl-Fischer assay has imidazole and sulfur dioxide in this solvent.
This compound is the heaviest component of the first known saddle azeotrope, which boils at 58Â°C. A mix of chloroform and this compound is used to extract lipids in the Folch method. It's added to cells at -20Â°C for fixing and permeabilization before flow cytometry. The most common titrant in a Karl-Fischer assay has imidazole and sulfur dioxide in this solvent. Coomassie Blue is delivered in a 50% solution of this compound and aqueous acetic acid, and along with Tris and glycine, it constitutes the transfer buffer for a Western blot. Either this compound or (*) acetonitrile is mixed with water to dissolve samples for ESI. When used to excess in reverse phase HPLC, this polar protic solvent dissolves the silica gel. When forming biodiesel, this compound reacts with fatty acids to make methyl esters. Oxidizing it gives formaldehyde, then formic acid. For 10 points, name this toxic one-carbon alcohol.
This compound is the heaviest component of the first known saddle azeotrope, which boils at 58Â°C.
methanol [or methyl alcohol; or CH3OH; or MeOH]