Denhart's solution is a mixture of polymers that saturate non-specific binding sites in this method.
Denhart's solution is a mixture of polymers that saturate non-specific binding sites in this method. Herring or salmon sperm is used as a blocking agent in this procedure. Large fragments are usually depurinated with hydrochloric acid prior to transfer. In this method, the result of agarose gel electrophoresis is transferred to a nylon or nitrocellulose sheet, where it is hybridized with a fluorophore-tagged or radiolabelled probe made of single-stranded DNA or RNA. The membrane is washed, leaving the hybridized target to be detected. This technique is the basis for RFLP analysis, and led to the development of a family of similar procedures. For 10 points, give this technique named for a British biologist that detects a specific DNA sequence in a sample.
Denhart's solution is a mixture of polymers that saturate non-specific binding sites in this method.
Southern blot