These things are secreted by HPGRT-positive hybrid myelomatous cells that are grown in a HAT medium; those cells constitute hybridomas.
These things are secreted by HPGRT-positive hybrid myelomatous cells that are grown in a HAT medium; those cells constitute hybridomas. In one assay that can be made more sensitive by adding haptens to the analytes, these structures can be visualized with streptavidin after being biotinylated or alternatively visualized with HRP. In another technique, proteins are labeled with a primary one and then a conjugate (*) monoclonal one of these things after being blotted on a polyvinylidene fluoride or nitrocellulose membrane. Those techniques are ELISA and the western blot. They undergo somatic hypermutation, in which their epitopes are modified, after B cells begin to proliferate during an immune response. For 10 points, name these Y-shaped proteins that bind to foreign antigens.
These things are secreted by HPGRT-positive hybrid myelomatous cells that are grown in a HAT medium; those cells constitute hybridomas.
antibodies [or immunoglobulins; accept Ig]