These proteins are antagonized by dam and dcm methylases, and the domain from one of these isfused to a zinc finger to generate targeted gene therapy constructs.
These proteins are antagonized by dam and dcm methylases, and the domain from one of these isfused to a zinc finger to generate targeted gene therapy constructs. Artificial plasmids have apolylinker site that is recognized by several different varieties of these proteins. They were originallydiscovered by Nathans, Smith, and Arber, and evolved in order to protect from viruses and foreigngenomes. They are used to map polymorphisms in the (*) RFLP technique. They can produce eitherblunt or sticky ends depending on whether they leave an overhang, and must always recognize palindromicregions. Commonly used examples include HindIII ("hin-dee-three") and EcoRI ("eeco-r-one"). For 10points, identify these enzymes that cleave DNA at specific sites.
These proteins are antagonized by dam and dcm methylases, and the domain from one of these isfused to a zinc finger to generate targeted gene therapy constructs.
restriction enzymes [or restriction endonucleases; prompt on partial answer]