To fix that and promote growth during this assay, microsomes isolated from rat liver are mixed in.
Among the improvements made to this procedure are the introduction of an rfa mutation that makes a lipopolysaccharide coat more permeable to a certain substance, and the introduction of the pkm10 plasmid, which carries SOS repair genes and ampicillin resistance. An alternative to this procedure that uses a liquid substrate and a 96-well microplate is called the fluctuation method. The man who developed this procedure also improved it by inactivating the uvrB gene during it. In its original form, this procedure failed with substances like benzopyrene, because cytochrome P450 is needed to activate it. To fix that and promote growth during this assay, microsomes isolated from rat liver are mixed in. This procedure basically works by incubating Salmonella typhimurium on agar that has been spread with a small amount of histidine. For 10 points, identify this eponymous biological test for a mutagenic agent.
Among the improvements made to this procedure are the introduction of an rfa mutation that makes a lipopolysaccharide coat more permeable to a certain substance, and the introduction of the pkm10 plasmid, which carries SOS repair genes and ampicillin resistance.
Ames test