Filip Pattyn and Jan Hellemans established standards for reporting data gained from this technique.
Filip Pattyn and Jan Hellemans established standards for reporting data gained from this technique. Solutions of betaine, formamide, and DMSO often increase the efficiency of this technique.  A trial and error procedure for reducing background noise in this procedure reduces one variable until optimal conditions are reached. One undesirable result of this technique is a kind of dimer. The most widely used variant of this technique uses SYBR ("cyber") Green or oligonucleotides tagged with fluorescent reporters. It requires magnesium cations, dNTPs, and a polymerase obtained from Thermus aquaticus. Temperature is varied during the denaturation, annealing, and elongation steps, which are cycled several times in, for 10 points, what technique with a "real-time" variety that uses a primer to amplify DNA?
Filip Pattyn and Jan Hellemans established standards for reporting data gained from this technique.
Polymerase Chain Reaction [accept quantitiative PCR or real time PCR until "trial and error"; prompt afterward until mentioned] 