One undesirable result of this technique is a kind of dimer.
Filip Pattyn and Jan Hellemans established standards for reporting data gained from this technique. Solutions of betaine, formamide, and DMSO often increase the efficiency of this technique.  A trial and error procedure for reducing background noise in this procedure reduces one variable until optimal conditions are reached. One undesirable result of this technique is a kind of dimer. The most widely used variant of this technique uses SYBR ("cyber") Green or oligonucleotides tagged with fluorescent reporters. It requires magnesium cations, dNTPs, and a polymerase obtained from Thermus aquaticus. Temperature is varied during the denaturation, annealing, and elongation steps, which are cycled several times in, for 10 points, what technique with a "real-time" variety that uses a primer to amplify DNA?
Filip Pattyn and Jan Hellemans established standards for reporting data gained from this technique.
Polymerase Chain Reaction [accept quantitiative PCR or real time PCR until "trial and error"; prompt afterward until mentioned] 