Cells lacking Aurora B kinase are stuck in this stage.
Probes contained in Yeast Artificial Chromosome are hybridized to denatured chromosomes of this kind in a form of fluorescence in situ hybridization. Cells can be harvested at this point via the addition of colcemide. Cells lacking Aurora B kinase are stuck in this stage. This stage ends with dephosphorylation and ubiquitination of securin, which allows separase to hydrolyze cohesin. Although this stage occupies only 4% of the total cell cycle, cells can be stuck there for days even with the formation of the namesake plate. In the stage just before this one, vesicles containing the dissolved nuclear envelope are formed. During it, the kinetochore microtubules push and pull on the chromosomes until they line up in the middle. For 10 points, name this mitotic stage followed by anaphase.
Probes contained in Yeast Artificial Chromosome are hybridized to denatured chromosomes of this kind in a form of fluorescence in situ hybridization.
metaphase 