Nonspecific interactions in this technique can be minimized by "pre-clearing" the lysate.
It's not CRISPR, but data from this technique is being used to populate the Cistrome Project. 4-thiouridine, 6-thioguanosine, and other photoreactive ribonucleoside analogs are incorporated into nascent RNA transcripts in a form of this procedure. Depending on the nature of the control, a variant of this technique can either be analyzed by calculating the fold enrichment or the percentage of input. Nonspecific interactions in this technique can be minimized by "pre-clearing" the lysate. A reagent in this technique is (*) immobilized on either superparamagnetic or agarose beads. Micrococcal nuclease shearing occurs in the "native" type of the best-known variety of this technique, whose starting product can also be sonicated after being "cross-linked" by adding UV light or formaldehyde. For 10 points, name this technique in which a protein is pulled out of solution using an antibody, whose "chromatin" form is abbreviated ChIP.
It's not CRISPR, but data from this technique is being used to populate the Cistrome Project.
immunoprecipitation [or ChIP until read; or CLIP until "ChIP" is read; or RIP until "ChIP" is read; accept answers that contain other tacked-on procedures, such as ChIP-Seq, as long as IP is mentioned in there] 