Tuschl established the existence of two separate phases in this phenomenon; these are the initiator phase that includes such proteins as Pasha and Drosha and the effector phase that takes place in the Argonaute-containing RISC complex.
In yeast, the effector phase of this phenomenon was determined to be dependent on GW182, a protein necessary for formation of discrete processing bodies, sometimes called decay centers. This phenomenon was first identified by Napoli, Lemieux, and Jorgensen, who were trying to determine if chalcone synthase was the rate-limiting step of anthocyanin biosynthesis. It was later confirmed by Ramano and Macino in Neurosopora, though they referred to it as quelling. Tuschl established the existence of two separate phases in this phenomenon; these are the initiator phase that includes such proteins as Pasha and Drosha and the effector phase that takes place in the Argonaute-containing RISC complex. Work in C. elegans by Fire and Mello demonstrated that this phenomenon functioned by means of a double-stranded construct, cut by the ribonuclease Dicer. For 10 points, identify this process in which the presence of specific double-stranded nucleic acids leads to the inhibition of gene expression.
In yeast, the effector phase of this phenomenon was determined to be dependent on GW182, a protein necessary for formation of discrete processing bodies, sometimes called decay centers.
RNA Interference [prompt on co-suppression; prompt on quelling before mentioned]