Stopped-flow competition experiments can be used to find burst-phase intermediates in this process, and its transition states are characterized in phi value analysis.
Stopped-flow competition experiments can be used to find burst-phase intermediates in this process, and its transition states are characterized in phi value analysis. This process can be analyzed via dual-polarization interferometry or monitored by the disappearance of the 225nm band in circular dichroism spectroscopy. Molecules that have undergone this process tend to have a lower hydrogen-deuterium exchange. Bryngelson and Wolynes applied the principle of minimal frustration to this process, leading to the important "energy landscape theory" of it. Often beginning with a random coil and passing through molten globule states, the kinetics of this process can be obtained from a chevron plot, and considering the time needed to undergo this process leads to Levinthal's paradox. Anfinsen used ribonuclease to study this process, which is assisted by GroEL-GroES, Hsp70, and other chaperonins. For 10 points, name this process that can be co- or post-translational, the configuration of a polypeptide into its 3-dimensional structure.
Stopped-flow competition experiments can be used to find burst-phase intermediates in this process, and its transition states are characterized in phi value analysis.
Protein {folding}