A trick when creating the matrix for this technique is to use a pipet tip to push bubbles away.
A trick when creating the matrix for this technique is to use a pipet tip to push bubbles away. Combining this technique with chloroquine is used to tell apart positive and negative supercoiling. Sanger sequencing involves a capillary version of this technique to read the fluorescent markers. This technique is performed before transferring to nitrocellulose paper in Southern Blotting. It is typically performed in tris-acetate buffer, and the (*) intercalating agent ethidium bromide is used to image the results under a UV light. A "ladder" is used as a marker in this technique, which separates fragments based on size. For 10 points, name this technique, in which a current applied to a DNA sample forces it to migrate through an agarose matrix.
A trick when creating the matrix for this technique is to use a pipet tip to push bubbles away.
gel electrophoresis