This technique can be used to monitor beta-galactosidase activity when using ONPG as a substrate.
This technique can be used to monitor beta-galactosidase activity when using ONPG as a substrate. This technique is the simplest way for a microplate reader to obtain data from ELISA. A protein solution is mixed with phosphomolybdate and phosphotungstate, then analyzed with this method in the Lowry assay. The purity of protein solutions can be estimated using this technique by obtaining a (*) 280/260 ratio as long as protein has typical amounts of tyrosine, tryptophan, and cysteine residues. Calibration curves prepared using this technique have a slope equal to the extinction coefficient. Quartz cuvettes are used in this technique when probing with wavelengths below 400 nm. Absorbances from this technique are related to concentrations by Beer's law. For 10 points, name this technique that analyzes how much light is absorbed by a solution.
This technique can be used to monitor beta-galactosidase activity when using ONPG as a substrate.
spectrophotometry [accept colorimetry; accept UV-Vis spectroscopy; accept ultraviolet-visible spectroscopy; accept absorption spectroscopy] 