Hoffmann-La Roche holds the patent on this process, and the first machine to automate this process, developed by Cetus, automatically added more enzyme since the enzyme was destroyed by the high temperature required to denature its substrate.  
Hoffmann-La Roche holds the patent on this process, and the first machine to automate this process, developed by Cetus, automatically added more enzyme since the enzyme was destroyed by the high temperature required to denature its substrate.  This problem was overcome by the use of Thermus  aquaticus, or Taq, enzyme, which would remain stable through many heating and cooling cycles. More recently there is also a "real time reverse transcription", or RT, version of this technique. For ten points, what is this method developed by Kary Mullis, used to amplify large quantities of DNA?
Hoffmann-La Roche holds the patent on this process, and the first machine to automate this process, developed by Cetus, automatically added more enzyme since the enzyme was destroyed by the high temperature required to denature its substrate.  
Polymerase Chain Reaction