SunTags were developed as fusions to this protein. The herpes simplex activation domain VP64, which is also the omega subunit of prokaryotic RNA polymerase, is most commonly used fused to this protein.
SunTags were developed as fusions to this protein. The herpes simplex activation domain VP64, which is also the omega subunit of prokaryotic RNA polymerase, is most commonly used fused to this protein. A conserved arginine cluster on this protein's bridge helix interacts with the "seed" region on its substrate. A KRAB domain is fused to this protein in an interference technique developed by Lei Qi. The D10A mutation applied to this enzyme makes it a (*) nickase, and another point mutation eliminates this protein's RuvC activity, forming its dead or "d" variant. Emmanuelle Charpentier engineered RGENs for this protein. This protein, extracted from Streptococcus pyogenes, binds specifically to PAM sequences. Jennifer Doudna [DOWD-nuh] observed that this protein, helped by guide RNAs, induces double-strand breaks with more efficiency than zinc-finger nucleases. For 10 points, name this endonuclease central to gene editing by CRISPR.
SunTags were developed as fusions to this protein. The herpes simplex activation domain VP64, which is also the omega subunit of prokaryotic RNA polymerase, is most commonly used fused to this protein.
Cas9 [or CRISPR-associated endonuclease-9; or CRISPR/Cas9; do not accept or prompt on "CRISPR" alone though] 