An improvement of this technique uses a glass substrate in a microfluidic cartridge, then moves the plate under an aperture in order to take measurements; this version allows easy application of compounds and is called "planar" .
An improvement of this technique uses a glass substrate in a microfluidic cartridge, then moves the plate under an aperture in order to take measurements; this version allows easy application of compounds and is called "planar" . Coating the apparatus with Sylgard 184 cuts back on noise in this assay, which often uses an automated perfusion apparatus. In another variation, gramicidin or amphothericin-B in the apparatus creates perforations in the target in order to get whole-cell information. Most versions of this technique rely on the formation of a gigaohm seal. Invented by Nobelists Sakhmann and Neher, for 10 points, name this electrophysiological assay that uses a specialized micropipette to record currents in sections of membrane.
An improvement of this technique uses a glass substrate in a microfluidic cartridge, then moves the plate under an aperture in order to take measurements; this version allows easy application of compounds and is called "planar" .
{Patch clamp}ing (Prompt on “Voltage clamping”)