The amylase inhibitor tendamistat is used as a scaffold in this technique.
The amylase inhibitor tendamistat is used as a scaffold in this technique. Georgiou et al. improved the specificity of this technique by coupling it to the twin-arginine translocation pathway. Dane Wittrup developed a variant of this technique using the Aga2p protein in yeast. Shotgun alanine scanning combines this technique with SDM. The goal of this technique developed by George Smith is to isolate a mimotope. This process, also called "biopanning," was used by AbbVie to (*) design HUMIRA. In this technique, a protein of interest is fused to pVIII ["P-eight"] from M13, expressed in E. coli, then the sample is flowed over the target and eluted. This technique, which is often done in several rounds using directed evolution, is helpful for identifying antibody fragments with good affinity at very high throughput. For 10 points, name this method in protein engineering in which a virus that infects bacteria expresses the desired protein on its surface.
The amylase inhibitor tendamistat is used as a scaffold in this technique.
phage display [or phage surface display; or bacteriophage display; or affinity maturation; or biopanning; prompt on partial answer]