This procedure uses a device containing a Peltier sensor in order to maintain the temperature ofthe sample.
This procedure uses a device containing a Peltier sensor in order to maintain the temperature ofthe sample. An "overlap" version of this procedure is used to join two halves of a construct via alinker, and this procedure is also the main step of site-directed mutagenesis. An antibody to the mainreagent of this procedure is added to the buffer in its "hot-start" variant. DMSO is used in thisprocedure to work with (*) GC-rich templates. The buffer in this procedure contains magnesium. Aprotein from Thermus aquaticus is commonly used in this procedure; that protein has evolved to survive inhot springs, and is called Taq polymerase. For 10 points, name this procedure used to amplify segments ofDNA, invented by Kary Mullis.
This procedure uses a device containing a Peltier sensor in order to maintain the temperature ofthe sample.
PCR [or polymerase chain reaction]