Researchers at Alnylam recently used it to lower cholesterol in mice by decreasing levels of apolipoprotein B. One of its effects is chromatin remodeling through histone deacetylation and DNA methylation.
Researchers at Alnylam recently used it to lower cholesterol in mice by decreasing levels of apolipoprotein B. One of its effects is chromatin remodeling through histone deacetylation and DNA methylation. Although most eukaryotes are capable of it, its pathway is notably absent in Saccaromyces cerevisiae. Mutation of the eri-1 gene enhances its activity in C. elegans, where it is particularly effective. Off-target effects are common, since it depends on homology to 20-25 mer oligonucleotides. These are produced through cleavage of a double-stranded precursor by dicer, and associate with RISC to cause endonuclease activity. For 10 points, name this mechanism of post- transcriptional gene silencing.
Researchers at Alnylam recently used it to lower cholesterol in mice by decreasing levels of apolipoprotein B. One of its effects is chromatin remodeling through histone deacetylation and DNA methylation.
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