Jaffrey and Page developed an RNA mimic of this protein called the 24-2 aptamer.
Jaffrey and Page developed an RNA mimic of this protein called the 24-2 aptamer. It's not ubiqtuitin or anything Gal4-related, but a split version of this protein is be used for bimolecular complementation assay as a replacement for FRET systems. This protein contains an HBI molecule that ionizes to a phenolate upon correct cyclization of the serine-65-tyrosine-66-glycine-67 motif. The calcium-binding protein Aqueorin associates with it in vivo. This protein contains a beta-barrel motif surrounding its active site. Assays with this protein commonly use a confocal microscope. This protein was discovered by Shimamura in jellyfish, and was refined by Tsien, who mutated it into several different colors. For 10 points, identify this useful reporter protein that glows its namesake color.
Jaffrey and Page developed an RNA mimic of this protein called the 24-2 aptamer.
GFP or Green fluorescent protein