Mva1 will induce this process even in Dam and Dcm-positive cells. This procedure is prevented from occur- ring at specific sites during footprinting assays. TALENs perform this process, and Cas9 catalyzes it during CRISPR. Nonspecificity in this procedure is called star activity. This procedure is done within a polylinker in the first step of molecular cloning. Proteins responsible for it may be isocaudomers or isoschizomers. The re- sults of this procedure are assessed with RFLP analysis. Immediately after it is done, an alkaline phosphatase can be added to prevent recircularization. HinD3 and EcoR1 carry out this process. It makes blunt ends or sticky ends for further ligation. For 10 points, name this process which is carried out by restriction endonucle- ases.
Mva1 will induce this process even in Dam and Dcm-positive cells. This procedure is prevented from occur- ring at specific sites during footprinting assays. TALENs perform this process, and Cas9 catalyzes it during CRISPR. Nonspecificity in this procedure is called star activity. This procedure is done within a polylinker in the first step of molecular cloning. Proteins responsible for it may be isocaudomers or isoschizomers. The re- sults of this procedure are assessed with RFLP analysis. Immediately after it is done, an alkaline phosphatase can be added to prevent recircularization. HinD3 and EcoR1 carry out this process. It makes blunt ends or sticky ends for further ligation. For 10 points, name this process which is carried out by restriction endonucle- ases.
Mva1 will induce this process even in Dam and Dcm-positive cells.
DNA restriction digest [or word forms; or DNA cleavage; or double-strand breaks; or DSBs; prompt on editing until it is read]