When performing this technique, you might set the instrument to collect a z-stack, then add rhodamine B or FITC to your sample.
In this technique, the data can be analyzed using a hidden Markov model in 3B analysis. Forms of this technique with long acronyms include gSHRIMP, SPDM-phy-mod, and PALMIRA. In one form of this technique, the problem of overlapping PSFs can be avoided by "stochastic switching." One form of this technique minimizes the effects of concentration-dependent scattering by measuring lifetime instead of intensity. When performing this technique, you might set the instrument to collect a z-stack, then add rhodamine B or FITC to your sample. An activation laser is followed by a readout laser in a form of this technique called PALM. The 2014 Nobel Prize in Chemistry was awarded for super-resolved variants of this technique, which can use a confocal setup to excite molecules in a localized region. For 10 points, name this imaging technique which observes singlet-to-singlet electronic relaxations.
In this technique, the data can be analyzed using a hidden Markov model in 3B analysis.
fluorescence microscopy [prompt on super-resolved microscopy or confocal microscopy]