A variant of this technique incorporates the process of competitive binding, while a novel variant is known as the "reverse" type and can be used in multi-targeted tests.
A variant of this technique incorporates the process of competitive binding, while a novel variant is known as the "reverse" type and can be used in multi-targeted tests. The initial samples in this laboratory technique are bound to the medium via surface absorption, which is followed by a nonspecific protein wash. Results of this test are confirmed with western blotting, and protein targets used in this technique are bound to another peptide that induces a color change, which can be detected via spectrophotometry or fluorogenesis. Those reporter proteins will generate a color proportional to the amount of sample antigen within the initial serum. For 10 points, name this common immunology lab technique based on the interactions between antibodies and antigens that is used to detect food allergens and which has a feminine acronym.
A variant of this technique incorporates the process of competitive binding, while a novel variant is known as the "reverse" type and can be used in multi-targeted tests.
ELISA or enzyme-linked immunosorbent assay (prompt on immunoassay)ELISA or enzyme-linked immunosorbent assay (prompt on immunoassay)