It can be used to determine the net charge of a protein at a given pH, however, its usefulness is limited in cases where dilute solutions are being used or if the reagents are far from neutral pH.
It can be used to determine the net charge of a protein at a given pH, however, its usefulness is limited in cases where dilute solutions are being used or if the reagents are far from neutral pH. The equation, which assumes no acid  dissociation or base hydrolysis, sets the logarithm of the quotient of a base and its conjugate acid plus its acid  dissociation constant (pKa) equal to pH. FTP, name this equation often used in the analysis of buffer solutions.
It can be used to determine the net charge of a protein at a given pH, however, its usefulness is limited in cases where dilute solutions are being used or if the reagents are far from neutral pH.
Henderson-Hasselbach equation