One protein in this pathway can be modified into a useful D10A nickase mutant and a double D10A/H840A mutant.
The Sharp Lab used this technology to screen a mouse model for metastatic mediators. The PX330 plasmid is provided by the Zhang Lab for this technique. Artificial versions of this system combine the tracrRNA and crRNA into one plasmid. One protein in this pathway can be modified into a useful D10A nickase mutant and a double D10A/H840A mutant. This pathway requires the sequence NGG on its (*) target molecule, which is called a protospacer adjacent motif or PAM. In this pathway, a short guide RNA binds to a target sequence, allowing the Cas9 nuclease to create a double-strand break. For 10 points, name this genome editing technology adapted from a prokaryotic defense mechanism.
The Sharp Lab used this technology to screen a mouse model for metastatic mediators.
CRISPR [or clustered regularly interspaced short palindromic repeats]