Exemplified by chromosomal crossover, for 10 points, name this process by which segments of DNA are cleaved and then joined together with other segments.
One commercial method for accomplishing this available from Invitrogen is the Clonase reaction, which is designed for use with the companion Gateway product. Conditional knock-in studies often use a type of this called the Cre-lox system, which may be induced using pIpC. It can be responsible for fusion genes, such as BCR-ABL and AML1-ETO, and one variety of it in antibodies makes use of RSSs on variable, diversity, and (*) joining regions. Another variety of it occurs during Prophase I at points known as chiasmata, while artificial means of performing it often make use of sticky ends to control the orientation of the fragments in the construct. Exemplified by chromosomal crossover, for 10 points, name this process by which segments of DNA are cleaved and then joined together with other segments.
One commercial method for accomplishing this available from Invitrogen is the Clonase reaction, which is designed for use with the companion Gateway product.
Genetic recombination [accept homologous recombination]