These cells are commonly cultured in the presence of Leukemia Inhibitory Factor, which induces the overexpression of (*) NANOG.
Inhibitors of ROCK kinase are commonly used when culturing these cells after single-cell dissociation. In maintenance of these cells, a group of polycomb proteins like EZH2, SUZ12, and EED are responsible for methylated H3K27 and repressing transcription. The SSEA-1 surface marker is commonly used in flow-cytometric analyses of these cells. One marker for these cells is a protein containing both a POU sequence and a homeodomain, which is capable of inducing expression of Rex1. These cells are commonly cultured in the presence of Leukemia Inhibitory Factor, which induces the overexpression of (*) NANOG. These cells form three-dimensional cylindrical bodies in some culture conditions. Oct4, Sox2, and Klf2 are also markers of these cells, which can be created by somatic cell nuclear transfer. The easiest way to acquire these cells is through the inner cell mass of a blastocyst. For 10 points, name this type of cell capable of developing into any tissue in the body.
Inhibitors of ROCK kinase are commonly used when culturing these cells after single-cell dissociation.
embryonic stem cells [or ESCs or induced pluripotent stem cells or iPS cells; prompt on “stem cells”]