For 10 points, name this process analogous to the Merrifield synthesis, in which a solid phase supports an iterated reaction cycle which forms phospho∙di∙ester bonds.
Modified forms of this process which incorporate sulfur into the product make use of reagents such as the Beaucage reagent or TETD. This process is the main industrial use of controlled pore glass. The efficiency of this process is monitored by measuring absorbance at 495 nanometers, since the DMT carbo∙cat∙ion used in it is bright orange in color. Marvin Caruthers and Leroy Hood developed the common method for this process, which entails a capping step where acetic an∙hydride and N-methyl∙imidazole react with two different alcohols before a pyri∙dinium iodide oxidation. A cyano∙ethyl protecting group is used during this process, in which tetrazole catalyzes a coupling reaction between phospho∙ramidite building blocks. For 10 points, name this process analogous to the Merrifield synthesis, in which a solid phase supports an iterated reaction cycle which forms phospho∙di∙ester bonds.
Modified forms of this process which incorporate sulfur into the product make use of reagents such as the Beaucage reagent or TETD.
solid-phase DNA synthesis [or oligonucleotide synthesis; or deoxyribonucleic acid synthesis; or primer synthesis; or RNA synthesis]