Burke's modification of this process adds sodium bicarbonate, while a modification of it using ammonium oxalate to prevent precipitation was developed by Thomas Hucker.
Burke's modification of this process adds sodium bicarbonate, while a modification of it using ammonium oxalate to prevent precipitation was developed by Thomas Hucker. One alternative to this process was developed by Ziehl and Neelsen and is used because this process is ineffective on acid-fast organisms. That modification uses a more basic compound in the final step to avoid adverse reactions to (*) safranin. It involves the use of acetone or alcohol as a decolorizer and iodine as a mordant, as well as crystal violet, which results in a purple colour in the presence of peptidoglycan in the cell wall. For 10 points, name this method for separating bacteria into "positive" and "negative" groups, a stain named for its Danish creator.
Burke's modification of this process adds sodium bicarbonate, while a modification of it using ammonium oxalate to prevent precipitation was developed by Thomas Hucker.
{Gram} stain