Analytes are assumed to not directly interact with the matrix in this technique.
Hayashi, Matsui, and Takagi developed a three-detector method for use with this technique, which uses static LS, UV, and RI in line with it. This technique is commonly used in desalting and buffer exchange or to remove radiolabelled dNTPs from polymerized DNA. Sephadex and sephacryl are common matrices used in this technique, and this technique is an alternative to asymmetric field-flow fractionation. It is commonly combined with a multi-angle light scattering apparatus as a detector, and can be used to find the polydispersity index of a polymer. This technique is used in biology to separate (*) oligomeric protein complexes from monomers and unfolded proteins from folded ones, and uses dextran blue or thyroglobulin to establish the void volume. Analytes are assumed to not directly interact with the matrix in this technique. This technique uses porous beads to hinder smaller molecules in the pores while allowing larger molecules to go around the pores and leave the column faster. For 10 points, name this form of chromatography which separates analytes by their hydrodynamic radius or molar mass.
Hayashi, Matsui, and Takagi developed a three-detector method for use with this technique, which uses static LS, UV, and RI in line with it.
size exclusion chromatography [or gel permeation chromatography or gel filtration chromatography; prompt on “chromatography” or “column chromatography”]