Originally developed by Merrifield, this procedure occurs by first functionalizing a resin and repeatedly coupling, washing, and deprotecting t-BOC or Fmoc conjugated monomers.
One reagent commonly used in this procedure is often replaced by Oxyma Pure because said replacement is not explosive, and replacing one reagent in this procedure with PyBOP eliminates the creation of the carcinogen HMPA. Products of this process can be processed further via a reaction that uses an intramolecular S-N-acyl shift after a trans-thio-esterification step. An HMP-containing reagent named for Wang is activated in the first step of this procedure. Using an uronium reagent in this process eliminates the need for an activation step by a carbo-di-imide like DCC. Triazole reagents are used to increase stereospecificity in one step of this procedure, which also uses groups like (*) Cbz and alloc. Originally developed by Merrifield, this procedure occurs by first functionalizing a resin and repeatedly coupling, washing, and deprotecting t-BOC or Fmoc conjugated monomers. This reaction is commonly carried out on beads in DMF solvent, which is washed out at every step as a new amino acid is added. For 10 points, name this chemical procedure which creates short proteins in vitro.
One reagent commonly used in this procedure is often replaced by Oxyma Pure because said replacement is not explosive, and replacing one reagent in this procedure with PyBOP eliminates the creation of the carcinogen HMPA.
Solid-phase peptide synthesis