This functionality of this enzyme is dependent on the association of Z alpha and Z omega subunits, and disruption of one of those can be used to perform the trademark blue/white screening assay to test for recombinant clones.
This molecule's Glu-461 serves as a general acid, while in E.Coli, Glu-537 serves as the nucleophile for a substitution reaction. An autosomal recessive disease resulting in dwarfism can result from a deficiency of this in humans and is called type B Morquio syndrome. Substrates for this enzyme include a glycosphingolipid that can accumulate and cause Tay-Sachs, ganglioside GM1, and induction of this protein's synthesis can be achieved via treatment with IPTG. This functionality of this enzyme is dependent on the association of Z alpha and Z omega subunits, and disruption of one of those can be used to perform the trademark blue/white screening assay to test for recombinant clones. For ten points, identify this hydrolase enzyme that catalyzes the breakdown of a certain polysaccharide into monosaccharides.
This molecule's Glu-461 serves as a general acid, while in E.Coli, Glu-537 serves as the nucleophile for a substitution reaction.
{beta-galactosidase}