One type of this procedure uses probes with quencher molecules which quench an attached fluorophore when excited through FRET.
One type of this procedure uses probes with quencher molecules which quench an attached fluorophore when excited through FRET. In this procedure with a real-time variant, the annealing step occurs at a lower temperature than the previous denaturation step. After the introduction of primers corresponding to sequences which flank the DNA region of interest, the namesake Taq enzyme extends the DNA strand complementary to the template. Developed by Kary Mullis, For 10 points, name this procedure used to amplify the number of copies of a specific DNA sequence from a sample.
One type of this procedure uses probes with quencher molecules which quench an attached fluorophore when excited through FRET.
{PCR} [accept {Polymerase Chain Reaction}]