Sephadex and Sephacryl are common matrices for this technique, which may use thyroglobulin or dextran blue as markers for calibrating the void volume.
One form of this technique uses calibration curves that rely on the Himmel-Squire method to determine the approximate spherical equivalents of random coils. Hayashi, Matsui, and Takagi developed another form of this technique that incorporates static LS, UV, and RI detectors as well as ASTRA multi-angle laser light scattering software. Fitting the data measured with this technique to the Mark-Houwink-Sakurada equation enables the calculation of polymer (*) molecular weights from intrinsic viscosities. Sephadex and Sephacryl are common matrices for this technique, which may use thyroglobulin or dextran blue as markers for calibrating the void volume. This technique can separate monomers from oligomers and folded from unfolded proteins because they have different hydrodynamic radii. For 10 points, name this type of chromatography in which larger molecules pass more slowly than smaller ones through a column of porous beads.
One form of this technique uses calibration curves that rely on the Himmel-Squire method to determine the approximate spherical equivalents of random coils.
size exclusion chromatography [or  gel permeation chromatography; or  gel filtration chromatography; or  molecular sieve chromatography; prompt on "(column) chromatography"]