The major in vitro types of this process are "random" and "site-directed."
An early method of performing a type of this process using an M13 phage to infect CJ236 E. coli, which lacks dut and ung; said method was developed by Thomas Kunkel. The sequence that determines the specificity of repressors of the 434 and P22 phages was studied using the "cassette" type of this process. Agilent's protocol for this process eliminates subcloning and rescue with the use of the DpnI ("d-p-n-one") restriction enzyme to chew up (*) wild-type DNA. The major in vitro types of this process are "random" and "site-directed." A chemical's ability to cause this process is assessed by the Ames test. In Drosophila, ethyl methane sulphonate is commonly used for this process due to its guanine alkylation activity. For 10 points, identify this process in which mutations are induced in a DNA sequence.
An early method of performing a type of this process using an M13 phage to infect CJ236 E. coli, which lacks dut and ung; said method was developed by Thomas Kunkel.
mutagenesis [until "mutations" is read, accept: inducing mutations, obvious equivalents]