This procedure can be repeated by stripping the substrate using pH 2.5 glycine solution, and that substrate is activated using methanol and washed in TBS-T solution.
This procedure can be repeated by stripping the substrate using pH 2.5 glycine solution, and that substrate is activated using methanol and washed in TBS-T solution. The confirmatory HIV test after an ELISA consists of using patient serum as a probe in this procedure. Non-fat dry milk is used as a blocking solution in this procedure, which commonly uses horseradish-peroxidase-conjugated molecules for visualization. This procedure has an ice-chilled (*) transfer step, which moves the products of an SDS-PAGE electrophoresis to a PVDF membrane. For 10 points, name this procedure in which an antibody is used to detect protein on a membrane, which was named as a pun on the DNA-based Southern blots.
This procedure can be repeated by stripping the substrate using pH 2.5 glycine solution, and that substrate is activated using methanol and washed in TBS-T solution.
Western Blot or immunoblot [prompt on word forms of "blotting" or "protein blotting"]