In some species, these bodies may be surrounded by segrosomes, and a convenient method of creating them is the LR/BP clonase gateway system.
In some species, these bodies may be surrounded by segrosomes, and a convenient method of creating them is the LR/BP clonase gateway system. Some contain an addiction mechanism via the Hok/Sok factors, while another set are referred to as "col". Alkaline lysis followed by column fractionation is one method of isolating them, while they may be introduced via cesium chloride, electroporation, or heat shock. Synthetic ones often contain a polylinker region and are extracted via the miniprep, and they were originally discovered by Joshua Lederberg in his studies of conjugation. Including the F-factor and R-factor, FTP, name these short, circular, extrachromosomal segments of DNA found in bacteria.
In some species, these bodies may be surrounded by segrosomes, and a convenient method of creating them is the LR/BP clonase gateway system.
{plasmid} (prompt on “vector”)