Quenching processes can reduce the quantum yield of emission from excited electronic singlet states studied by this method.
Diffusive systems can be examined with this technique by observing the recovery after photobleaching, while resonant energy transfer studies require the use of a pair of dyes such as NBD, rhodamine, or GFP. Quenching processes can reduce the quantum yield of emission from excited electronic singlet states studied by this method. For ten points, name this optical technique that probes its namesake spectral emission with lifetimes on the order of 10 to the negative 8 seconds.
Diffusive systems can be examined with this technique by observing the recovery after photobleaching, while resonant energy transfer studies require the use of a pair of dyes such as NBD, rhodamine, or GFP.
Fluorescence spectroscopy