An arginine and a glutamate residue may catalyze cyclization reactions in the Ser65–Tyr66–Gly67 sequence in this protein's beta barrel structure.
The addition to this protein of a Kozak translation initiation site by changing its second residue to valine has improved the expression of it in human tissues. An A206K mutation in it stops it from dimerizing, and certain pH-sensitive mutants of this protein are called super-ecliptic pHluorins. Other variants of it are particularly useful for (*) FRET experiments. An extra double bond differentiates this protein from a variant of it isolated from members of the genus Discosoma. An arginine and a glutamate residue may catalyze cyclization reactions in the Ser65–Tyr66–Gly67 sequence in this protein's beta barrel structure. Chalfie, Shimomura, and Tsien received the Nobel Prize in Chemistry in 2008 for their work with this protein, which was first isolated from Aequorea victoria, a type of jellyfish. FTP, name this protein, often used as a reporter, that fluoresces when exposed to blue light.
The addition to this protein of a Kozak translation initiation site by changing its second residue to valine has improved the expression of it in human tissues.
{G}reen {F}luorescent {P}rotein