When working with basement membranes, adding a chaotropic agent like NaSCN during this assay improves its fidelity, and Rheumatoid factor causes high levels of false positives in it. Reagents undergo a checkerboard titration before this assay, and Czerzinsky developed a version of it used to measure cytotoxic T-cell responses and identify cytokine-producing cells, known as its "SPOT" version. Another version is performed by first attaching one capture molecule to the substrate, known as the "sandwich" version. Often performed following a blocking step with BSA or casein and visualized using urease, alkaline phosphatase, or HRP, the more standard version of this assay requires the creation of
When working with basement membranes, adding a chaotropic agent like NaSCN during this assay improves its fidelity, and Rheumatoid factor causes high levels of false positives in it. Reagents undergo a checkerboard titration before this assay, and Czerzinsky developed a version of it used to measure cytotoxic T-cell responses and identify cytokine-producing cells, known as its "SPOT" version. Another version is performed by first attaching one capture molecule to the substrate, known as the "sandwich" version. Often performed following a blocking step with BSA or casein and visualized using urease, alkaline phosphatase, or HRP, the more standard version of this assay requires the creation of a standard curve, known as the "indirect" version. Used in HIV testing or when a western blot is insufficient, for 10 points, name this assay performed in a microtiter plate, which uses antibodies to quantify the amount of antigen in a sample.
When working with basement membranes, adding a chaotropic agent like NaSCN during this assay improves its fidelity, and Rheumatoid factor causes high levels of false positives in it.
{ELISA} or {EIA} or {Enzyme}-linked {immuno}sorbent {assay}