Polyhistidine tagged proteins are purified using divalent cations in a variation of this technique. Band broadening in this procedure is equal to the sum of terms related to the eddy diffusion, latera
Polyhistidine tagged proteins are purified using divalent cations in a variation of this technique. Band broadening in this procedure is equal to the sum of terms related to the eddy diffusion, lateral diffusion, and resistance to transfer, a result known as Van Deemter's equation. It can be sped up by pumping nitrogen gas through the apparatus, a variation called "flash" (*), and sepharose is used in the size exclusion variant of this procedure. DEAE or CM is used to separate particles by charge in the ion-exchange type. FTP, name this laboratory technique that separates an eluent by running it through a glass tube containing a stationary phase.
Polyhistidine tagged proteins are purified using divalent cations in a variation of this technique.
{Column Chromatography} (accept {Affinity chromatography} until *, prompt on {chromatography})