This technique can be used to determine the number of microsatellite repeats in a sample. When there is excessive nonspecific activity, this procedure is modified with a gradual ramp-down of a certain parameter. Practitioners of this technique generally use a "master mix" which contains magnesium chloride and dNTPs. The products of this technique can be visualized by staining with ethidium bromide
This technique can be used to determine the number of microsatellite repeats in a sample. When there is excessive nonspecific activity, this procedure is modified with a gradual ramp-down of a certain parameter. Practitioners of this technique generally use a "master mix" which contains magnesium chloride and dNTPs. The products of this technique can be visualized by staining with ethidium bromide and running a gel. This procedure is conducted in thermocyclers, which can change the temperatures for the denaturation, annealing, and elongation stages. For 10 points name this technique that uses the heat-stable "Taq" polymerase to amplify target DNA sequences.