Jim Hasseloff and colleagues discovered an 84-nt cryptic intron in this protein's cDNA sequence, making codon alteration a necessary step in this protein's expression in Arabidopsis. Well-characterized mutations of this protein include a serine-to-threonine mutation at position 65 described by Roger Heim, which can be combined with a phenylalanine-to-leucine mutation at position 64 to generate a super-ecliptic pH-sensitive variant of this molecule. The structure of this molecule consists of a beta barrel, and its maturation involves modificatio
Jim Hasseloff and colleagues discovered an 84-nt cryptic intron in this protein's cDNA sequence, making codon alteration a necessary step in this protein's expression in Arabidopsis. Well-characterized mutations of this protein include a serine-to-threonine mutation at position 65 described by Roger Heim, which can be combined with a phenylalanine-to-leucine mutation at position 64 to generate a super-ecliptic pH-sensitive variant of this molecule. The structure of this molecule consists of a beta barrel, and its maturation involves modification of a serine-tyrosine-glycine tripeptide that makes up its chromophore. In 2008, Martin Chalfie and Roger Tsien were awarded the Chemistry Nobel for their work with this molecule, whose major emission peak occurs at 509 nanometers. First isolated from the Aequoria victoria jellyfish, for 10 points, name this molecule often used as a biological marker that when exposed to blue light, emits its namesake colored light.
Jim Hasseloff and colleagues discovered an 84-nt cryptic intron in this protein's cDNA sequence, making codon alteration a necessary step in this protein's expression in Arabidopsis.
green fluorescent protein [accept GFP]