One assay for measuring this entity's activity involves production of the yellow byproduct orthonitrophenol from ONPG. In vivo, IPTG can be used to directly induce this entity's activity, which can then be monitored by breakdown of X-gal. This entity's structural elements produce permease and transacetylase enzymes. Cyclic AMP binds to catabolite a
One assay for measuring this entity's activity involves production of the yellow byproduct orthonitrophenol from ONPG. In vivo, IPTG can be used to directly induce this entity's activity, which can then be monitored by breakdown of X-gal. This entity's structural elements produce permease and transacetylase enzymes. Cyclic AMP binds to catabolite activator protein in the positive regulation of this genetic construct, which also produces the enzyme beta-galactosidase. Its activity is negated in the presence of large amounts of glucose because glucose can substitute as a carbon source for its namesake sugar. For 10 points, identify this operon regulating the breakdown of that sugar, which is found in milk.
One assay for measuring this entity's activity involves production of the yellow byproduct orthonitrophenol from ONPG.
lac operon [accept beta-galactosidase until “induce”]