The first of these proteins to be discovered was isolated from Haemophilus influenzae, and, like many of its class, that example is nonfunctional when its target is methylated. Only one of their three types does not require ATP to function, and two of their types require AdoMet to function. Analysis by (*) Southern blot may require a sample to be digested by these proteins, common examples of which are Xbal and EcoRI. These proteins create either sticky or blunt ends when they cleave at certain sequences, which are often palindromic. For 10 points, name this class of enzyme often used in experiments to cleave DNA at specific sites.
The first of these proteins to be discovered was isolated from Haemophilus influenzae, and, like many of its class, that example is nonfunctional when its target is methylated. Only one of their three types does not require ATP to function, and two of their types require AdoMet to function. Analysis by (*) Southern blot may require a sample to be digested by these proteins, common examples of which are Xbal and EcoRI. These proteins create either sticky or blunt ends when they cleave at certain sequences, which are often palindromic. For 10 points, name this class of enzyme often used in experiments to cleave DNA at specific sites.
The first of these proteins to be discovered was isolated from Haemophilus influenzae, and, like many of its class, that example is nonfunctional when its target is methylated.
{restriction} enzymes [or {restriction endonucleases}]