An algal form of this molecule tagged with a Venus reporter has been used by the Svoboda group to map layer 2 and 3 callosal projections. Stability of its active intermediate form depends on protonation of a conserved glutamine residue that forms a salt bridge with a conserved arginine residue, as well as the transfer of a proton from a Schiff base linkage with lysine to another glutamine residue. Arrestin binds its phosphorylated domain to deactivate it, and at high calcium concentrations, recoverin inhibits its kinase
An algal form of this molecule tagged with a Venus reporter has been used by the Svoboda group to map layer 2 and 3 callosal projections. Stability of its active intermediate form depends on protonation of a conserved glutamine residue that forms a salt bridge with a conserved arginine residue, as well as the transfer of a proton from a Schiff base linkage with lysine to another glutamine residue. Arrestin binds its phosphorylated domain to deactivate it, and at high calcium concentrations, recoverin inhibits its kinase. Its activation of the G protein transducin allows a phosphodiesterase to hydrolyze cyclic GMP, closing sodium-calcium channels to hyperpolarize the cell. It is broken down in the pigment epithelium and reconverted to a complex including an 11-cis form in the outer segment disks. One of its subunits selects for its spectrum; the other is a chromophore made of an aldehyde of vitamin A. Converting to all-trans form when a photon hits, for 10 points, name this photopigment found in rods.
An algal form of this molecule tagged with a Venus reporter has been used by the Svoboda group to map layer 2 and 3 callosal projections.
{rhodopsin}