The converse of this process reacts a F-Moc-protected unit with a resin and the next DCC-activated unit. It can be repeated for only 50 cycles, necessitating pre-treatment with cyanogen bromide. The amino (*) end reacts with phenyl isothiocyanate, releasing a phenylthiohydantoin derivative of the single N-terminal amino acid. Improving on Sanger degradation, for 10 points—
The converse of this process reacts a F-Moc-protected unit with a resin and the next DCC-activated unit. It can be repeated for only 50 cycles, necessitating pre-treatment with cyanogen bromide. The amino (*) end reacts with phenyl isothiocyanate, releasing a phenylthiohydantoin derivative of the single N-terminal amino acid. Improving on Sanger degradation, for 10 points— name this method of sequencing proteins.
The converse of this process reacts a F-Moc-protected unit with a resin and the next DCC-activated unit.
Edman degradation