An improvement of this technique uses a glass substrate in a microfluidic cartridge, then moves the plate under an aperture in order to take measurements; this version allows easy application of compounds and is called "planar" . Coating the apparatus with Sylgard 184 cuts back on noise in this assay, which often uses an automated perfusion apparatus. In another variation, gramicidin or amphotheri
An improvement of this technique uses a glass substrate in a microfluidic cartridge, then moves the plate under an aperture in order to take measurements; this version allows easy application of compounds and is called "planar" . Coating the apparatus with Sylgard 184 cuts back on noise in this assay, which often uses an automated perfusion apparatus. In another variation, gramicidin or amphothericin-B in the apparatus creates perforations in the target in order to get whole-cell information. Most versions of this technique rely on the formation of a gigaohm seal. Invented by Nobelists Sakhmann and Neher, for 10 points, name this electrophysiological assay that uses a specialized micropipette to record currents in sections of membrane.
An improvement of this technique uses a glass substrate in a microfluidic cartridge, then moves the plate under an aperture in order to take measurements; this version allows easy application of compounds and is called "planar" .
{Patch clamp}ing (Prompt on “Voltage clamping”)