The site-specific variety was first characterized in 1968 by Smith, Wilcox, and Kelley, and today over 900 different ones have been isolated from 230 strains of bacteria. The Type I variety cleaves at a site over 1000 base pairs away from the recognition site, while the more
The site-specific variety was first characterized in 1968 by Smith, Wilcox, and Kelley, and today over 900 different ones have been isolated from 230 strains of bacteria. The Type I variety cleaves at a site over 1000 base pairs away from the recognition site, while the more commonly used Type II enzymes cut within the recognition site. Named for the strain of bacteria and order of discovery from that bacteria, commonly used examples include EcoRI (Eee-koh-are-won) and HindII (hind-two). FTP, name this type of enzyme, which is used to digest DNA and which has allowed the growth of recombinant DNA technology.
The site-specific variety was first characterized in 1968 by Smith, Wilcox, and Kelley, and today over 900 different ones have been isolated from 230 strains of bacteria.
restriction enzymes or restriction endonucleases