First, flood the heat-fix
First, flood the heat-fixed smear with crystal violet for one minute. Next, add iodine solution for 10 minutes. Then briefly decolorize with alcohol, and finally counterstain with safranin. After this process, the cells whose cell walls consist chiefly of peptidoglycan will be purple, while the negative cells appear pinkish-red. FTP, identify this test used to classify bacteria on the basis of their cell walls.
First, flood the heat-fixed smear with crystal violet for one minute.
{Gram} Stain or {Gram}Test