Precision mapping of CpG island methylation via this technique can be used to determine the state of an isolated chromatin sample. Exon and intron location is mapped using the RT-version of this, which includes a step using reverse transcriptase to generate a cDNA before the annealing step. The thermal denaturation step is eliminated in the helicase-dependent variety, and the extension step involves raising the temperature to ensure optimal Taq enzyme activity. The "tail" variant of this uses a 
Precision mapping of CpG island methylation via this technique can be used to determine the state of an isolated chromatin sample. Exon and intron location is mapped using the RT-version of this, which includes a step using reverse transcriptase to generate a cDNA before the annealing step. The thermal denaturation step is eliminated in the helicase-dependent variety, and the extension step involves raising the temperature to ensure optimal Taq enzyme activity. The "tail" variant of this uses a nested pair of primers with different annealing temperatures, and non-specific background interference is reduced in its "touchdown" variety. For ten points, identify this technique involving in-vitro enzymatic amplification of DNA.
Precision mapping of CpG island methylation via this technique can be used to determine the state of an isolated chromatin sample.
{P}olymerase {C}hain {R}eaction