This procedure can be repeated by stripping the substrate using pH 2.5 glycine solution, and that substrate is activated using methanol and washed in TBS-T solution. The confirmatory HIV test after an ELISA consists of using 
This procedure can be repeated by stripping the substrate using pH 2.5 glycine solution, and that substrate is activated using methanol and washed in TBS-T solution. The confirmatory HIV test after an ELISA consists of using patient serum as a probe in this procedure. Non-fat dry milk is used as a blocking solution in this procedure, which commonly uses horseradish-peroxidase-conjugated molecules for visualization. This procedure has an ice-chilled (*) transfer step, which moves the products of an SDS-PAGE electrophoresis to a PVDF membrane. For 10 points, name this procedure in which an antibody is used to detect protein on a membrane, which was named as a pun on the DNA-based Southern blots.
This procedure can be repeated by stripping the substrate using pH 2.5 glycine solution, and that substrate is activated using methanol and washed in TBS-T solution.
Western Blot or immunoblot [prompt on word forms of "blotting" or "protein blotting"]