A cell may not progress past this stage if there is not enough tension on spindle fibers.
This process includes the Mad2 protein, which binds to Cdc20, an activator for the next stage after this process. A cell may not progress past this stage if there is not enough tension on spindle fibers. Colchicine is used to arrest this stage so that karyotypes can be made from it, since chromosomes are at their most coiled. Before passing the spindle checkpoint during it, each sister chromatid must be attached via a kinetochore to a spindle fiber. In it, chromosomes line up on the equatorial plate of the cell. For 10 points, name this middle stage of mitosis succeeded by anaphase.
This process includes the Mad2 protein, which binds to Cdc20, an activator for the next stage after this process.
Metaphase [prompt on {mitosis} until mentioned]