Steps of this process include [*] denaturing, during which the template strands detach, annealing, during which primers attach, and elongation, during which thermostable Taq ["tack"] polymerase adds new nucleotides to synthesize a complementary strand.
One variety of this process can use SYBR green florescent dye to make it quantitative in real-time. Because Pfu has three-prime to five-prime proofreading ability, its use in this process results in increased accuracy over an enzyme that produces three-prime adenosine overhangs. Steps of this process include [*] denaturing, during which the template strands detach, annealing, during which primers attach, and elongation, during which thermostable Taq ["tack"] polymerase adds new nucleotides to synthesize a complementary strand. Invented by Kary Mullis, for 10 points, identify this process by which a strand of DNA is amplified.
One variety of this process can use SYBR green florescent dye to make it quantitative in real-time.
{polymerase chain reaction} [accept prefixation of “real time,” “quantitative,” “RT,” or “Q,” before mentioned]