David Whitcomb developed Scorpion molecules for use in this assay, which can also use a reporter that has a FAM fluorescent reporter tethered to a TAMRA or MGB quencher.
David Whitcomb developed Scorpion molecules for use in this assay, which can also use a reporter that has a FAM fluorescent reporter tethered to a TAMRA or MGB quencher. Dimers can be detected by adding a dissociation stage at the end of this assay and searching for spurious peaks. A common method of analyzing the data in this assay is the REST software, or if efficiency is set to 1 the delta-delta Ct method is sufficient. GAPDH or Hexokinase are commonly used housekeeping genes in this assay, which also employs either Taqman probes or SYBR green fluorescent dye. Operating on the assumption that a more abundant DNA molecule will amplify more quickly, for 10 points, name this variation of PCR often used in gene expression studies, which is capable of assaying for the amount of transcript or sequence in a sample.
David Whitcomb developed Scorpion molecules for use in this assay, which can also use a reporter that has a FAM fluorescent reporter tethered to a TAMRA or MGB quencher.
{RT}-{PCR} [or {Real time} PCR or {Quantitative} PCR or {qPCR} or any combination of those {two}, prompt on {PCR}; or {quantitative real time polymerase chain reaction}]