These do not function well at high glycerol concentrations, which cause a loss of specificity, an effect known as star activity.
These do not function well at high glycerol concentrations, which cause a loss of specificity, an effect known as star activity. They do not work on methylated substrates, allowing them to inhibit bacteriophage growth without harming the bacteria themselves. They can produce blunt ends, or if there is an overhang, sticky ends. The first one to be discovered was HindIII, and other notable examples include TaqI and EcoRI. They are used in genetic engineering to produce recombinant DNA, or to prepare DNA fragments for use in gel electrophoresis. For 10 points, name this type of enzyme that cuts DNA at specific recognition sequences.
These do not function well at high glycerol concentrations, which cause a loss of specificity, an effect known as star activity.
{restriction enzymes} or {endonucleases}