Foster resonance energy transfer between fluorogenic dyes allows for quantification of the products of this technique in a real-time variant.
Foster resonance energy transfer between fluorogenic dyes allows for quantification of the products of this technique in a real-time variant. This technique consists of denaturation, annealing, and elongation steps, and was developed by Kary Mullis. The bacterium Thermus aquaticus provides a necessary heat-stable DNA polymerase known as Taq. The presence of free primers and nucleotides is also necessarily in this technique. For 10 points, name this biological technique used to create numerous copies of segments of DNA.
Foster resonance energy transfer between fluorogenic dyes allows for quantification of the products of this technique in a real-time variant.
{PCR} [or polymerase {chain reaction}]