Its development is partly controlled by the lin-4 miRNA, and mutants include dumpy, roller, and unc.
Sarin and Hobert used Illumina sequencing in these organisms instead of positional cloning to identify mutants in a forward screen, and mutagenesis in these organisms is usually carried out by adding EMS to their growth media. Their gonads can be microinjected in order to create knockout lines, and RNAi constructs can be fed to these organisms via E. Coli. Its development is partly controlled by the lin-4 miRNA, and mutants include dumpy, roller, and unc. They can shift into a dormant dauer state but usually go through the larval stages L1 through L4 before hitting maturity. Used by Horvitz to study apoptosis, these organisms were popularized by Brenner. Occuring as only hermaphrodites or males, every cell in the adult has been fate-mapped. For 10 points, name this common model organism, a nematode.
Sarin and Hobert used Illumina sequencing in these organisms instead of positional cloning to identify mutants in a forward screen, and mutagenesis in these organisms is usually carried out by adding EMS to their growth media.
{C Elegans} or Caenorhabditis elegans