Using a pump in place of gravity and gradient elution are two differences in the high-performance variant of this technique.
C8 and C18 are commonly used in a variety of the process with reversed phase, while dynamic light scattering can be combined with absolute size-exclusion to determine the size of molecules in this process. Using a pump in place of gravity and gradient elution are two differences in the high-performance variant of this technique. Affinity tails such as His-tags or antibodies can be used in this process to select specific biological compounds, and an (*) ion-exchange variant works similarly. Zeolite, silica gel and activated alumina are commonly used to slow elution in this process, wherein each element of a mobile phase percolates through the stationary phase at a different rate. For 10 points, name this laboratory technique used to separate mixtures that comes in both gas and liquid varieties.
C8 and C18 are commonly used in a variety of the process with reversed phase, while dynamic light scattering can be combined with absolute size-exclusion to determine the size of molecules in this process.
{Column Chromatography}