Progressively reducing temperature over the course of this technique serves to remove unwanted background in its touchdown variant.
One way to quantify this procedure's results is the delta-delta Ct method, where Ct is the threshold. The proofreading ability of Pfu is sometimes preferred to another reagent in this technique. Progressively reducing temperature over the course of this technique serves to remove unwanted background in its touchdown variant. Determining the initial amount of sample can be done by generating a series of logistic curves to compare to internal standards in its real-time variant. This procedure goes through denaturation, annealing, and elongation steps through several cycles, and reagents required for it include forward and reverse primers, as well as dNTPs, and a certain enzyme denoted Taq. For 10 points, name this technique used to amplify DNA, developed by Kary Mullis.
One way to quantify this procedure's results is the delta-delta Ct method, where Ct is the threshold.
{polymerase chain reaction} [accept {real}-{time PCR} or {RT}-{PCR} until “{touchdown}”]