Used diagnostically in some introductory lab courses for the purposes of determining the orientation of an inserted gene, it is also used in site-directed mutagenesis and in quantitative measurement of a specific mRNA in tissue.
One serious drawback to this method is its high sensitivity, as it tends to produce many false positives in an unclean lab. Used diagnostically in some introductory lab courses for the purposes of determining the orientation of an inserted gene, it is also used in site-directed mutagenesis and in quantitative measurement of a specific mRNA in tissue. Cooling is necessary for rehybridization and for attachment of the primers to the original or newly synthesized DNA strands, but near-boiling temperatures will still not denature a certain enzyme of Thermus aquaticus, commonly known as Taq [TACK]. FTP, name this technique developed for large scale amplification of DNA, first proposed by Kary Mullis in 1983.
One serious drawback to this method is its high sensitivity, as it tends to produce many false positives in an unclean lab.
polymerase chain reaction