First discovered in C. elegans by Mello and Fire, this is, FTP, which process, previously known as quelling, in which gene expression is inhibited by cleaving the associated messenger RNA?
Saccharomyces cerivisiae notably lacks the pathway for this process, and Makarovna et al suggest that CRISPR are a prokaryotic analogue to it. One complex involved in this process, which was initially discovered by Rich Jorgensen in petunias, contains argonaute proteins, and that complex's formation is catalyzed by an endonuclease with a two-base overhang on its three prime site. That complex, known as RISC, is catalyzed by Dicer. First discovered in C. elegans by Mello and Fire, this is, FTP, which process, previously known as quelling, in which gene expression is inhibited by cleaving the associated messenger RNA?
Saccharomyces cerivisiae notably lacks the pathway for this process, and Makarovna et al suggest that CRISPR are a prokaryotic analogue to it.
RNA interference