It can be applied to calculate the net charge on a protein at a given pH and is considered an approximation of the law of mass action applied to one species.
One quantity in this equation can be expressed as one minus theta over theta where theta is the Langmuir adsorption isotherm. It can be applied to calculate the net charge on a protein at a given pH and is considered an approximation of the law of mass action applied to one species. This equation is less useful in extremely dilute solutions and when dealing with extremely strong acids or bases, and it ignores the minor dissociation of water and also hydrolysis of bases. For 10 points, identify this equation which relates the log of the ratio of conjugate base over acid and pKa to pH which is useful in characterizing buffer systems.
One quantity in this equation can be expressed as one minus theta over theta where theta is the Langmuir adsorption isotherm.
Henderson-Hasselbalch Equation