Total internal reflection microscopy is often used in the single-molecule version of this technique, which popularly uses a malemide-based reaction to tag cysteine residues.
Total internal reflection microscopy is often used in the single-molecule version of this technique, which popularly uses a malemide-based reaction to tag cysteine residues. Its efficiency is directly proportional to an overlap integral dependent on the fourth power of wavelength, and that calculation usually assumes an orientation factor of 2/3, meaning freely rotating dipoles. Cy3 and Cy5 are a popular pair used in this technique, which even more commonly uses CFP and YFP. Dipole-dipole coupling allow transfer of an excited chromophore's energy to an acceptor chromophore in this technique, causing a detectable shift in color when two molecules are close together. For 10 points, name this technique commonly used to screen for protein-protein interactions, in which two molecules are labelled with different dyes and then imaged.
Total internal reflection microscopy is often used in the single-molecule version of this technique, which popularly uses a malemide-based reaction to tag cysteine residues.
FRET or [Forster Resonance Energy Transfer; or Fluorescent Resonance Energy Transfer]