Its T664P mutant is known as the "O-helix mutant," while the substitution of phenylalanine for valine at position 667 promotes chain termination by this protein.  
Its T664P mutant is known as the "O-helix mutant," while the substitution of phenylalanine for valine at position 667 promotes chain termination by this protein.  A commonly used purification approach for this enzyme involves a one-hour incubation at 75 degrees followed by cation exchange chromatography.  This enzyme may be used in a non-cyclic process to prepare inserts into Topo-linked vectors, and an eponymous probe system relies upon the separation of fluors from quenchers by this enzyme's 5' to 3' exonucleolytic activity.  Unlike similar proteins, this enzyme leaves overhanging As, which is useful in the aforementioned TA cloning process but makes it a worse choice for blunt-end cloning than Pfu. For 10 points, name this PCR reagent, a high-fidelity polymerase derived from Thermus aquaticus.
Its T664P mutant is known as the "O-helix mutant," while the substitution of phenylalanine for valine at position 667 promotes chain termination by this protein.  
Taq polymerase