For 10 points, name this alternative to radioimmunoassay for identifying and measuring the amount of proteins, often pathogens, in a fluid sample.
Bai et al. covalently bonded PEI to a PMMA substrate in producing a microchannel-based device for performing this technique. Reagents used in this procedure often undergo a checkerboard titration, and some forms of it exploit the biotin/avidin system. The output of this procedure is typically read as percent of optical density, and the three main types are liquid phase, indirect, and sandwich. Though ?galactosidase and alkaline phosphatase are sometimes used, horseradish peroxidase gives the fastest results due to its high turnover number. It involves a chromophore attached to a nonspecific antibody, which attaches to a specific antibody that is already bound to an antigen. For 10 points, name this alternative to radioimmunoassay for identifying and measuring the amount of proteins, often pathogens, in a fluid sample.
Bai et al. covalently bonded PEI to a PMMA substrate in producing a microchannel-based device for performing this technique.
ELISA [or enzyme-linked immunosorbent assay; or enzyme immunoassay; or EIA]