The RACE type of this process is a special case of the anchored type, which utilizes a universal linker.
The RACE type of this process is a special case of the anchored type, which utilizes a universal linker. The hot-start version separates the components before the first step and the touch-down version decreases hybridization temperatures over time. The ARMS system utilizes the allele-specific type, which relies on single nucleotide distinctions near the three-prime end. Its product usually has an adenosine three-prime overhang and enzymes from Pyrococcus furiosus have been used in it thanks to their three prime to five prime exonuclease proofreading, but VENT and taq pol are also common. FTP, name this technique taking about thirty cycles; a DNA amplification method invented by Kary Mullis.
The RACE type of this process is a special case of the anchored type, which utilizes a universal linker.
polymerase chain reaction