For 10 points, name this process through which the primary structure of a polypeptide is determined.
One method for accomplishing this employs a Curtius rearrangement followed by the hydrogenolysis of the resulting benzyl carbamate. This process can benefit from labeling with Sanger's reagent or dansyl chloride. One method for accomplishing this ends each step with the cleavage of a thiazolinone; that adduct resulted from the reaction of phenylisothiocyanate with the N-terminal residue; those processes are the Bergmann and Edman degradations. Anfinsen's hypothesis suggests that determining this allows the reconstruction of tertiary structure. For 10 points, name this process through which the primary structure of a polypeptide is determined.
One method for accomplishing this employs a Curtius rearrangement followed by the hydrogenolysis of the resulting benzyl carbamate.
{protein sequencing}