For 10 points, HindIII [hin d three] and EcoRI [eeko r one] are what types of bacterial enzymes that are useful for cleaving DNA at specific sites in biological experiments?
Deviation from optimal pH or ionic strength results in star activity in this class of proteins. Dcm and Dam methylation in bacteria introduces resistance to the activity of these proteins, which are used to analyze RFLPs. Engineered constructs with multiple cloning sites can be acted on by many of these enzymes. Examples of these enzymes common to digests are XbaI [x b a one] and BamHI [bam h one], and the action of these enzymes results in the formation of either blunt or sticky ends. For 10 points, HindIII [hin d three] and EcoRI [eeko r one] are what types of bacterial enzymes that are useful for cleaving DNA at specific sites in biological experiments?
Deviation from optimal pH or ionic strength results in star activity in this class of proteins.
restriction enzymes [or restriction endonucleases]