For 10 points, name this chemical technique
 which involves passing a mobile phase through a stationary phase to separate a mixture.
Excluding sample preparation, two of these processes make up the first two phases of MudPit protein
 analysis. This process can be described with an equation that contains terms for eddy-dffusion, longitudinal
 diffusion and mass transfer called the van Deemter equation. A common material used in these setups is
 sepharose. It uses antibodies or other binding molecules in its affinity variant and a charged resin in its ion
 exchange form. The retention time is used to characterize this technique, which typically makes use of a
 column in its gas, liquid or high performance liquid variants. For 10 points, name this chemical technique
 which involves passing a mobile phase through a stationary phase to separate a mixture.
Excluding sample preparation, two of these processes make up the first two phases of MudPit protein
 analysis.
chromatography