The amount of target DNA doubles after each cycle.
Fluorescent dyes can be used for quantification in its real-time variety. It uses a thermocycler to change the temperature in a heat block, and incorporation of didNTPs stops elongation when it is used for sequencing. It replaced Southern hybridization for forensic analysis, and the reverse-transcriptase variety can be used to synthesize double-stranded cDNA. It requires two primers, dNTPs, and Taq polymerase, and involves cycling through denaturing, annealing, and extension steps. The amount of target DNA doubles after each cycle. Discovered by Kary Mullis, for 10 points, name this lab technique used to amplify short stretches of DNA.
Fluorescent dyes can be used for quantification in its real-time variety.
{polymerase chain reaction} or {PCR}