Variations of this laboratory technique can be used to determine the methylation pattern of DNA, create point mutations, and assemble multiple pieces of DNA.
Techniques similar to this laboratory technique include NEAR, which uses a nicking enzyme to bypass one of the steps normally needed for this technique, and HDA, which uses helicase to bypass that same step. NEAR and HDA are also notably different from this technique because they can be performed at a constant temperature. Variations of this laboratory technique can be used to determine the methylation pattern of DNA, create point mutations, and assemble multiple pieces of DNA. Important reagents for this technique include a divalent cation, such as magnesium, and a thermostable polymerase, such as Pfu or Taq. This reaction occurs in a thermocycler, which rotates the reaction between the denaturation, annealing, and elongation temperatures. For 10 points, name this technique developed by Kary Mullis that is used to amplify DNA.
Techniques similar to this laboratory technique include NEAR, which uses a nicking enzyme to bypass one of the steps normally needed for this technique, and HDA, which uses helicase to bypass that same step.
{Polymerase Chain Reaction}