For 10 points, name this assay used to detect apoptosis in fixed cells, whose name is an acronym for Terminal Transferase dUTP Nick-end Labeling.
One negative control used in this assay is a Leukemic cell line treated with campothecin, and Kraupp et al treated rat liver cells with CPA and NAF to show that the original formulation of this assay was non-specific.  One more sensitive alternative to this procedure uses FTIC conjugated to Annexin V alongside propium iodide, while another alternative to this assay uses Hoescht 33258 and 7-amino-actinomycin. Cells that are positive for this assay will usually also exclude the dye tryphan blue, and this procedure can be combined with flow cytometry by using a monoclonal antibody against Br-dU. Originally described by Garvrieli, Sherman, and Ben-Sasson, this assay uses a TdT ? enzyme to add a particular substrate to double-strand breaks. For 10 points, name this assay used to detect apoptosis in fixed cells, whose name is an acronym for Terminal Transferase dUTP Nick-end Labeling.
One negative control used in this assay is a Leukemic cell line treated with campothecin, and Kraupp et al treated rat liver cells with CPA and NAF to show that the original formulation of this assay was non-specific.  
{TUNEL} Assay [accept {Terminal Transferase dUTP Nick}-{end Labeling} until mentioned]