All of the reactions were conducted using a final volume of 50 µl that contained 1X of PCR buffer, 3 mM MgCl2, 400 µM dNTP, 25 pmol of each primer, 1 U of Taq Polymerase Invitrogen, Life Technologies and 25 ng of DNA from the cell culture infected with C. abortus strain A22 that was utilized as a control or 25 ng of DNA from the L929 line mouse fibroblast cells.
Las reacciones se llevaron a cabo en un volumen de 50 µl con amortiguador de PCR 1X, MgCl2 3 mM, dNTP 400 µM, 25 pmol de cada primer, 1 U de Taq Polimerasa Invitrogen, Life Technologies y 25 ng de ADN del cultivo infectado con la cepa ATCC de C. abortus A22 como testigo positivo o bien con el cultivo de fibroblastos de ratón L929 sin infectar.