They were later placed in cellular culture Petri boxes. They were covered with 30,000 cells and 200 µL of DNEM/F12 medium Dulbecco's modified Eagle basal medium supplemented with calf fetus serum at 10% Grand Island Biological Company, Grand Island, New York, USA.
Posteriormente fueron depositados en cajas de Petri para cultivos celulares cubriéndolos con 30,000 células y 200 uL del medio DNEM/F12 Dulbecco's modified Eagle's basal medium suplementado con suero al 10% de feto de becerro Grand Island Biological Co. Grand Island NY, USA.