The stems and roots were superficially disinfected according to the description of and . They were sectioned in 1 cm segments to be cultivated in potato dextrose agar PDA and were incubated at room temperature 25°C. The resulting fungal isolates, or oomycetes, were purified in PDA or V8 medium, according to the specific case.
Los tallos y las raíces se desinfectaron superficialmente de acuerdo a lo descrito por y , se seccionaron en segmentos de 1 cm para sembrarlos en medio papa dextrosa agar PDA y se incubaron a temperatura ambiente 25 °C. Los aislamientos de hongos u oomicetos resultantes fueron purificados en PDA o medio V8, según el caso.