All samples were immersed in a cyclic pH system pH 4.4 to 7.0 and retrieved after 5, 10 and 15 days in order to be observed. Samples were examined under polarized light and Tholuet solution in a photomicroscope Zeiss, Germany in order to determine remineralization according to exhibited birefringence.
Las muestras fueron inmersas en un sistema de pH cíclico pH 4.4 a 7.0 y recuperadas a 5, 10 y 15 días para ser observadas bajo luz polarizada con solución de Tholuet en un fotomicroscopio y determinar la remineralización de acuerdo con la birrefringencia que mostraron.