Fragments of tissue from diseased plants of approximately 0.5 cm were desinfected in a sodium hypochlorite solution at 1.5 % for 2 min. Later, they were rinsed three times with sterile distilled water, dried with sterilized paper towels, transferred to PDA culture medium, and incubated at 24±2 °C under controlled conditions for a week; the growth of each organism was then quantified.
Fragmentos de tejido de plantas enfermas de aproximadamente 0.5 cm se desinfestaron y sumergieron en una solución de hipoclorito de sodio al 1.5 % durante 2 min, posteriormente se enjuagaron tres veces con agua destilada estéril, se secaron con papel absorbente estéril, se transfirieron a medio de cultivo PDA y se incubaron a 24±2 °C en condiciones controladas durante una semana, se cuantificó el desarrollo de cada organismo.