Samples were then stirred vigorously for 45-60 s, and serial dilutions in RTF were prepared. 100 µl was taken from the sample and placed in an eppendorf tube with 900 µl of RTF.
El procedimiento consistió en la agitación rigurosa durante 45 - 60 s y posteriormente se realizaron diluciones seriadas en RTF.Se tomaron 100 µl de la muestra y se colocaron en un eppendorf con 900 µl de RTF.