Each cycle lasted for approximately one minute, during which time samples were at a maximum temperature of 50 C ± 5 during 18 seconds and at a minimum temperature of 5 C during an additional 18 seconds.
Cada ciclo duró un tiempo aproximado de un minuto, durante el cual, las muestras estuvieron a una temperatura máxima de 50 C ±5, durante 18 segundos y a una mínima de 5 C durante otros 18 segundos.