Blood samples were collected from all patients. They were measured for Hb normal value >12g/dl for women, and >13g /dl for men, leukocytes normal value between 4,500/ mm3 and 10,000/mm3 , platelets normal value between 150,000/mm3 and 400000/mm3 and mean corpuscular volume MCV normal values between 80 fl. and 100 fl. Serum levels of vitamin B12 vit.B12 were determined using electrochemiluminescence ECLIA- Roche. This test is a competition assay in which the sample competes with added vitamin B12 labeled with biotin. Purified intrinsic factor is used. Levels were considered to be abnormal when they were 211 pg/ml or 156 pmol/L. For Patients with vitamin B12 deficiency, the presence of parietal cell antibodies PCA was identified by indirect immunofluorescence IFI-Bio Rad. The test was considered to be positive when titers were 1:10 or greater.
En todos los pacientes se tomaron muestras de sangre y se midieron: la hemoglobina Hb que se consideró un valor normal > de 12 gr/dl y de 13 gr/dl para mujeres y hombres respectivamente, leucocitos valor normal entre 4.500 y 10.000 mm, plaquetas valor normal entre 150.000 y 400.000 mm, volumen corpuscular medio VCM con valor normal entre 80 y 100 fl; se determinaron los niveles séricos de vitamina B12 vitamina B12 por la técnica de electroquimioluminiscencia ECLIA-Roche, que es un ensayo de competición en donde la muestra compite con la vitamina B12 añadida marcada con biotina, utiliza factor intrínseco purificado; se consideraron anormales niveles inferiores a 211 pg/ml ó 156 pmol/L. A los pacientes con deficiencia de vitamina B12 se les determinaron las presencia de anticuerpos contra células parietales ACP por la técnica de inmunofluorescencia indirecta IFI-BioRad, se consideraron positivos los títulos iguales o mayores de 1:10.