At the end of the incubation procedure, the plates without coverslips were stored at -70°C; the plates with coverslips had the MEM-C removed and were washed 3 times with a phosphate saline solution PBS, for 5 min on each occasion.
Pasado este tiempo, las placas sin cubreobjetos se conservaron a -70 °C; las placas con cubreobjetos se lavaron tres veces con PBS, durante cinco minutos cada vez.