In order to verify that there were no inhibitors in the ARN samples, one segment of the 18S gen was amplified using the specific primers 18SF 5´- ACG GAT CGC ACG GCC TTC GT -3 and 18SR 5´- ACC AGA CTT GCC CTC CAA TGG -3 .
Para descartar la presencia de inhibidores, se amplificó un segmento del gen 18S con los iniciadores específicos 18SF 5´- ACG GAT CGC ACG GCC TTC GT -3 y 18SR 5´- ACC AGA CTT GCC CTC CAA TGG -3 .