1995. The strain was grown in TSB-Amp for 48 h. A total of 100 μL of the culture was transferred to 5 mL of either TSB or TSB-Amp and incubated for 48 h to obtain passage 1.
La cepa se hizo crecer en CST-Amp durante 48 horas; de este cultivo, 100 μL se transfirieron a 5 mL de CST o CST-Amp y se incubaron por 48 horas para obtener el pasaje 1.