Cells in the logarithmic growth phase were harvested, and 1 x 10 cells were transfected by elec-troporation with 15 μg of the pBBR4-CMV-Ggp-SV40+ plasmid. A pulse of 140 V for 25 msec was applied to a 0.2-cm cell containing 0.1 mL of cellular suspension.
Las células se cosecharon hasta la fase logarítmica, se transfectaron 1 x 10 células por electroporación con 15 μg del plásmido pBBR4-CMV-Ggp-SV40+ y se aplicó un pulso de 140 V durante 25 mseg en una celda de 0.2 cm que contenía 0.1 mL de la suspensión celular.