For the present study, 30 extracted, single rooted premolars were used. Root canal treatment was performed on the teeth which were later stored in bi-distilled water at 37 C. Teeth were randomly allotted to three groups, all groups were comprised of 10 teeth.
En este estudio se emplearon 30 dientes premolares unirradiculares extraídos a los que se les realizó tratamiento de conductos y almacenándolos en agua bidestilada a 37 C. Se distribuyeron los dientes en tres grupos de forma aleatoria, con diez dientes por cada grupo.