Cross sections of 5 mm of diseased plant material were obtained, disinfected superficially with 1 % sodium hypchlorite, rinsed with sterile distilled water and planted in Petri dishes with B King. The dishes were incubated at 28 °C for 48 hours; the culture formed was transferred to a new culture medium, using an inoculation loop, using the Streak Plate Method.
De material vegetal enfermo se obtuvieron secciones de 5 mm, desinfectadas superficialmente en hipoclorito de sodio al 1 %, se lavaron con agua destilada estéril y se sembraron en cajas Petri con medio B de King, las cajas se incubaron a 28 °C durante 48 horas, de las colonias formadas con una asa bacteriológica se realizaron transferencias a nuevo medio de cultivo mediante la técnica de estría cruzada.