Samples were incubated during the following periods: 24, 72 and 168 hours at 37 °C with 6 rpm agitation. Once these periods elapsed, they were washed with PBS NaCl 8 g, KCl 2 g, KH2PO4 2 g, KH2PO4 - 12H2O 2.89 g and 1 liter distilled H2O pH 7.4 to remove non adhered cells.
Se incubaron durante los siguientes tiempos: 24, 72 y 168 h a 37 °C con agitación 6 rpm. Transcurridos estos tiempos las muestras se lavaron con PBS NaCl 8 g, KCl .2 g, KH2PO4 2 g, KH2PO4-12H2O 2.89 g, H2ODest 1 litro, pH 7.4 para remover las células no adheridas.