Then, the sample was thawed and cultivated in Brucella agar supplemented with 5% lamb's blood. Samples were incubated for 5 days in humid microaerophilic media at 370C 18.
Posteriormente, se descongeló la muestra y se cultivó en agar Brucella suplementado con sangre de cordero al 5% e incubado durante 5 días en un medio microaerofílico húmedo y a 37 °C 18.