In order to obtain a cellular line with CEMP1 protein cellular expression, these cells were selected with hygromycin B 600 μg/mL, during 8 weeks, and protein expression was determined as was explained before in this paper.
Para obtener una línea celular con expresión estable de la proteína CEMP1, estas células fueron seleccionadas con higromicina B 600 μg/mL, durante 8 semanas y la expresión de la proteína determinada como se detalla arriba.