100 µl was taken from this tube and placed in another tube with 900 µl 2nd dilution: 1:100.100 µl was taken and streaked in base agar medium with blood with menadione and hemin, and incubated for 14 days at 37ºC in absolute anaerobiosis.
Se tomaron 100 µl y se sembraron en medio agar base con sangre con menadiona y hemina, y se incubaron durante 14 días a 37ºC en anaerobiosis estricta.