For the liquid culture of the phytopathogenic fungi, circles of 1 cm in diameter were taken from each one of the fungal strains of a culture from 7 days of incubation, and were aseptically transferred to 250 mL Erlenmeyer flasks with 50 mL of potato broth with dextrose, where they incubated at 25 ± 2 °C for 15 days shaking them continuously at 150 rpm. Once the incubation period was over, the biomass produced by each fungal strain was filtered by vacuum filtration. Subsequently, it was dehydrated through lyophilization and was extracted with a mixture of methanol:chloroform 1:1 during 3 days, the solvent excess was eliminated through distillation with reduced pressure .
Para el cultivo líquido de los hongos fitopatógenos, se tomaron círculos de 1 cm de diámetro de cada una de las cepas fúngicas de un cultivo de 7 días de incubación, y se transfirieron asépticamente a matraces Erlenmeyer de 250 mL con 50 mL de caldo de papa y dextrosa, donde se incubaron a 25 ± 2 °C por 15 días manteniéndose en agitación constante a 150 rpm. Una vez concluido el periodo de incubación, se filtró al vacío la biomasa producida para cada cepa fúngica, posteriormente se deshidrató mediante liofilización y se extrajo con una mezcla metanol: cloroformo 1:1 durante 3 días, el exceso de disolvente se eliminó por destilación a presión reducida .