Rats were housed in individual cages in a temperature-controlled room with alternating 12 h light-dark cycles and were acclimated to the laboratory for 2 weeks before the study. Food was removed 12 h prior to the study, but all the animals were allowed free access to water.
Antes del estudio, las ratas se alojaron en jaulas individuales en una sala a temperatura controlada, con ciclos alternantes de 12 h de luz y oscuridad; todos los animales tuvieron acceso libre al agua.