The new plas-mid construct was characterized by enzyme restriction: BamHI linearized it, and XbaI released the G gene. The recombinant plasmid was called pBBR4-CMV-Ggp-SV40+ and was purified using the EndoFree Plasmid Giga Kit Qiagen, Hilden, Germany following the manufacturer's instructions.
El nuevo plásmido se caracterizó mediante restricción enzimática: BamHI lo linearizó y XbaI liberó el gen G. El plásmido recombinante se llamó pBBR4-CMV-Ggp-SV40+ y se purificó utilizando el sistema Endo Free Giga Plasmid Kit Qiagen, Hilden, Germany, siguiendo las instrucciones del fabricante.