Amp-resistant Ampr colonies were analyzed by PCR using pBBR primers 5'-GTAAAACGACGGCCAGT-3' and 5'-CGAGGTCGACGGTATCG-3' to amplify a 5100-bp fragment, corresponding to the insert ligated in pBBR1MCS-4.
Las colonias resistentes a ampicilina Ampr se analizaron mediante PCR usando los primers 5'-GTAAAACGACGGCCAGT-3' y 5'-CGAGGTCGACGGTAT-CG-3' para amplificar un fragmento de 5100 pb, que corresponde al inserto ligado en el plásmido pBBR1MCS-4.