From each isolate, sclerotia were taken, along with part of the tissue that displayed symptoms of the disease; they were disinfected with sodium hypochlorite at 1%, and then planted in Potato Dextrose Agar PDA for eight days at 20±2 °C for S. minor; and at room temperature for S. sclerotiorum and S. cepivorum.
De cada aislado se tomaron esclerocios y parte de tejido que presentó síntomas de la enfermedad, se desinfestaron con hipoclorito de sodio al 1 %, y se cultivaron en Papa Dextrosa Agar PDA durante ocho días a 20±2 °C para S. minor; y a temperatura ambiente de laboratorio para S. sclerotiorum y S. cepivorum.