Protein samples were boiled for 10 min at 100 ° C; they were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE and transferred to a nitrocellulose membrane.
Las muestras de proteína se hirvieron durante lOmin a 100 °C; luego se separaron con electroforesis en gel de dodecil sulfato-policrilamida sódica SDS.PAGE y se transfirieron a una membrana de nitrocelulosa.