Afterwards, the tubes were centrifuged at 3 000×g for 40 min at 4°C; then, 500 µl of the supernatant was extracted and transferred to a sterile microtube that was labeled and frozen at a temperature of -70°C to perform the isolation procedure.
Posteriormente, los tubos se centrifugaron a 3000 x G durante 40 minutos a 4 °C. Se tomaron 500 µl del sobrenadante y se transfirieron a un microtubo estéril, que se identificó y congeló a -70 °C hasta su utilización para el aislamiento bacteriológico.