Ten minutes before procedure, approximately 20 cc of the patient's blood were extracted, to obtain plasma rich in growth factors, as described in Anitua s protocol. It was done in the following fashion: The patients blood was placed in sterile tubes with 3.8% sodium citrate vacutainer as anticoagulant. Plasma was then centrifuged at 1800 RPM during 8 minutes Model BTI PRGF system II.
Diez minutos antes de comenzar procedimiento quirúrgico se extrajo aproximadamente 20 cc. de sangre del paciente para obtener plasma rico en factores de crecimiento según el protocolo descrito por Anitua como sigue: La sangre del paciente se colocó en tubos estériles con citrato sódico al 3.8% vacutainer como anticoagulante, posteriormente se centrifugó el plasma a 1,800 RPM durante 8 minutos BTI Modelo PRGF System II.