Before undertaking thermocycling procedure, samples were immersed in distilled water for 24 hours at constant 37 C temperature in an oven Felisa, Mexico. This procedure is called by some authors zero thermo-cycling.
Antes de realizar el termociclado, las muestras se sumergieron en agua destilada por 24 horas a una temperatura constante de 37 C en un horno Felisa México, lo que algunos autores llaman termociclado cero.