Western blots were performed to determine whether DLM-2 cells transfected with CEMP1 gene expressed this marker. For this aim, monoclonal Anti-His-6X C-term, Invitrogen, Carlsbad CA antibodies were used. Policlonal anti hrCEMP1 antibodies were also used. They were used to identify recombining protein histidines and specifically recognize CEMP1 protein respectively.
Para determinar si las células DML-2 transfectadas con el gen CEMP1 expresaban este marcador, se llevaron a cabo Western blots utilizando anticuerpos, tanto monoclonal Anti-His 6X C-term; Invitrogen, Carlsbad, CA y policlonal anti hrCEMP1, los cuales se utilizaron para identificar las histidinas de la proteína recombinante y para reconocer específicamente la proteína CEMP1, respectivamente.