The actinomycetes were seeded as macro-colonies on Agar Czapek Dox pH 7.2 and incubated at 28 °C for 7 d. The phytopathogenic bacteria were reactivated in nutrient broth plus 1% glucose w/v, pH 7.0, and then inoculated 10µL 0.5 McFarland scale into 3 mL of liquefied semisolid agar, tempered at 50 °C and then added quickly on the actinomycetes cultures.
Los actinomicetos fueron sembrados como macrocolonias en Agar Czapeck Dox pH 7.2 e incubadas a 28 ºC por 7 d. Las bacterias fitopatógenas fueron reactivadas en caldo nutritivo + 1% de glucosa p/v, pH 7.0, y posteriormente inoculadas 10 µL 0,5 escala Mac Farland a 3 mL de agar semisólido licuado y temperado a 50°C y luego agregado rápidamente sobre los cultivos de los actinomicetos.