For the isolation, purification and characterization of colonies, five calyxes were taken at random for each sample and were cut into pieces of approximately 1 cm2; they were disinfected with 1 % sodium hypochlorite for 1.5 to 2 minutes and rinsed three times with sterile distilled water; they were dried with sterile paper towels; subsequently four pieces were sown in Petri dishes with PDA culture medium brand Bioxonr.
Para el aislamiento, purificación y caracterización de colonias se tomaron cinco cálices al azar por cada muestra y se cortaron en trozos de 1 cm aproximadamente, se desinfestaron con hipoclorito de sodio al 1 % por 1.5 a 2 minutos y se enjuagaron tres veces con agua destilada estéril, se secaron con papel absorbente estéril y se procedió a sembrar cuatro trozos por caja Petri en medio de cultivo PDA marca Bioxonr.