The sample was composed of 30 single- rooted or bi-rooted teeth, donated by orthodontists. Teeth were previously cleansed and x-rayed, to assess whether they were free of caries, fissures, fractures or root calcifications. Teeth were stored in 9% isotonic saline solution and refrigerated until they were used.
Se recolectaron 30 dientes unirradiculares y birradiculares sanos donados por ortodoncistas, previamente limpios y radiografiados para verificar que se encuentran libres de caries, fisuras, fracturas o calcificaciones radiculares, almacenados en solución salina isotónica al 0.9% y refrigerados hasta ser utilizados.