Each reaction mixture 25 µl contains 1.0X PCR buffer 10 mM Tris-HCl, 50 mM KCl, pH 8.3, 1.5 µl of 2.5 mM dNTPs, 1 mM MgCl2, 10 pmol of each oligonucleotide Table 1, Figs. 1, 2, 3 and 1.2 U of Taq DNA polymerase and 5 ng of the metagenoma extracted from each sample and 2 ng of the genome of the control strains NanoDrop 2000.
Cada mezcla de reacción 25 µl contiene buffer 1.0X PCR 10 mM Tris-HCl, 50 mM KCl, pH 8.3, 1.5 µl de 2.5 mM dNTPs, 1 mM MgCl2, 10 pmol de cada oligonucleótido Tabla 1, Figuras 1, 2, 3 y 1.2 U de la Taq ADN polimerasa y 5 ng del metagenoma extraído de cada muestra y 2 ng del genoma de las cepas controles NanoDrop 2000.