Then, all the premolar roots were varnished and their coronal surfaces were immersed in 2% fuchsine dye solution for 24 hours. Samples were placed in resin blocks to be bucco-lingually sectioned with a Hamco Machine Thin Sectioning Machine cutter with a 0.5 mm thick High TechProducts. In Diamond Waterine Blade 65-10,005 diamond disk.
En seguida se cubrieron con barniz todas las raíces de los premolares y fueron inmersos, en la parte coronal, en una solución de fucsina al 2% por 24 h. Las muestras se colocaron en bloques de resina acrílica, para ser seccionadas bucopalatino, en una recortadora Thin Sectioning Machine, Hamco Machine , con disco de diamante de 0.5 mm de espesor, High TechProducrs. iN Diamond Waterine Blade 65 – 10005.