In 2001 Majalca et al. evaluated a base media of gelose and chocolate GC to which was added 2% freeze dried hemoglobin, Campylobacter agar, Casman agar, Columbia agar, brain-heart infusion agar, Brucella agar, Mueller Hinton agar or trypticase soy agar. Each mixture was supplemented with 7% to 10% of horse or lamb blood plus antibiotics with or without nicotinamide-adenine to a final concentration of 15 ug/mL. A microaerophilic environment was generated using system sachets and 3 Alka-Seltzer tablets in 10 mL of water. All of these components were placed inside of a flask sealed with plastic paraffin film with or without the use of a candle.
Majalca y cols 2001 evaluaron los medios base de GC gelosa, chocolate con 2% de hemoglobina liofilizada, agar Campylobacter, agar de Casman, agar de Columbia, agar infusión cerebro corazón, agar Brucella, agar Mueller Hinton y agar tripticasa soya, todos adicionados con 7-10% de sangre de caballo o de cordero suplementados con antimicrobianos y con o sin NAD Nicotinamida-adenina a una concentración de 15 ug/ml. El ambiente microaerofílico se logró utilizando sobres generadores de CO2 y empleando tres pastillas de Alkaseltzer en 10 ml de agua dentro de un frasco sellado con parafilm, con o sin vela.