4 lesions were inflicted in 60 crowns with a needle in the median stem of the crown, 30 of the crowns were placed in a recipient with 300 ml of the suspension of spores, 30 crowns were used as controls and were only added sterile water without zoospores.
En las 60 coronas se realizaron 4 lesiones con aguja en el tallo medio de la corona, 30 de las coronas se colocaron en recipiente con 300 ml de la suspensión de esporas, 30 coronas se utilizaron como testigos y solamente se le adicionó agua destilada estéril sin zoosporas.