In order to discard the presence of fungi and phytopathogenic bacteria, the symptomatic cladodes were directly observed through a stereo microscope and were dissected in 1 cm fragments that were spread on PDA and King B media.
Para descartar la presencia de hongos y bacterias fitopatógenas, los cladodios sintomáticos se observaron directamente en un microscopio estereoscópico y se disectaron en fragmentos de 1 cm que se sembraron en PDA y B de King.