After this time, 50 µL of tetrazolium Sigma were added to all wells, which were then incubated for 24 h. A color change from yellow to purple was interpreted as bacterial growth.
Al cabo de este tiempo, se agregó 50 µL de tetrazolium Sigma a todos los pocillos y se incubaron durante 24 h. Un cambio de color del amarillo al púrpura fue interpretado como crecimiento de bacterias.