In addition, two nested PCR protocols were implemented to investigate OHB frequency in cases that were positive for isolated anti-HBc and in the 10% of cases that were negative for all three markers.
Adicionalmente, se implementaron dos protocolos de PCR anidados para investigar la frecuencia de HBo en los casos positivos para anti-HBc aislado y en el 10% de los negativos para los tres marcadores.