The specimens were collected through centrifugation at 16,000 g for 5 minutes and the pellet was resuspended in 200 µL of lysis buffer TSNT 2 % Triton X-100, 1% SDS, 100 mM NaCl, 10 mM, TrisHCl and 1mM EDTA, pH 8, 500 µL of saturated phenol with Tris-HCl and 100 µL of a mixture of chloroform: amyl alcohol 24:1.
Los especímenes se recolectaron por centrifugación a 16,000 g por 5 min, y el pellet se resuspendió en 200 µL de amortiguador lisis TSNT 2 % Tritón X-100, 1 % SDS, 100 mM NaCl, 10 mM Tris-HCl y 1 mM EDTA, pH 8, 500 µL de fenol saturado con Tris-HCl y 100 µL de una mezcla cloroformo: alcohol isoamílico 24:1.