Since total bilirubin can be measured by accelerators that can break hydrogen bridges VdB, the difference between total bilirubin measured by VdB and conjugated bilirubin is considered to be non conjugated bilirubin.
La bilirrubina total puede ser medida por aceleradores que rompen puentes de hidrogeno VdB y la diferencia entre estas dos reacciones se considera la bilirrubina no-conjugada.