PCR was carried out in a TC020-24 MultiGene Mini Labnet International, Inc., Edison, NJ thermal cycler, with a final volume of 25 µL containing 1X buffer GoTaq PC Promega, Madison, WI, 200 ng of genomic DNA, 200 µM of each dNTPs, 0.5 µM of each initiator, sterilized water and 1 U of GoTaq DNA Polymerase Promega, Madison, WI.
La PCR se realizó en un termociclador TC020-24 MultiGene Mini Labnet Iternational, Inc., Edison, NJ en un volumen final de 25 µL conteniendo 1X buffer GoTaq PCR Promega, Madison, WI, 200 ng de ADN genómico, 200 µM de cada dNTPs, 0.5 µM de cada iniciador, agua estéril y 1 U de GoTaq DNA polimerasa Promega, Madison, WI.