These isolates were recuperated from Chalgren Wilkins agar supplemented with horse serum DIFCO, 7% Vitox Oxoid SR0091 and antibiotic supplement for H. pylori Oxoid SR0147. Cultures were placed in an incubator of CO2 under microaerophilic conditions of humidity and temperature sufficient for the growth of the cultures. Bacteria were identified by Gram staining and urease, catalase and oxidase reactions. Preparation of Bacteria.
Estos aislados se recuperaron en agar Wilkins Chalgren, suplementado con suero de caballo al 7% DIFCO, Vitox OXOID SR0091, y suplemento antibiótico para H. pylori OXOID SR0147, los cultivos fueron colocados en incubadora de CO2 en condiciones de microaerofilia, humedad y temperatura adecuadas para su crecimiento, las bacterias fueron identificadas por la coloración de Gram y las reacciones de ureasa, catalasa y oxidasa.