In order to determine whether cells transfected with CEMP1 gene expressed this protein, immune-location trials of these cells were undertaken. These trials were as follows: DML-2 cells were planted at low density 5 x 10 in eight-well, Lab-tek sterile boxes. Cells were left to adhere overnight and were cultivated for three days.
Para determinar si las células transfectadas con el gen CEMP1 expresaban esta proteína, se llevaron a cabo ensayos de inmunolocalización en estas células como se describe a continuación: las células DML-2 fueron sembradas a baja densidad 5x102 en cajas estériles Lab-Tek de ocho pozos; se dejó a las células adherirse durante toda la noche y se cultivaron durante tres días.