It was incubated for 40 min at 65 °C, and later, 700 μL of chloroform/isoamylalcohol were added 24:1; it was mixed and centrifuged for 8 min at 14,000 rpm. The supernatant was transferred to a new tube containing 800 μL of absolute ethanol.
Se incubó durante 40 min a 65°C, posteriormente, se adicionaron 700 μL de cloroformo/alcohol isoamílico 24:1, se mezcló y se centrifugó por 8 min a 14,000 rpm. El sobrenadante se transfirió a un tubo nuevo que contenía 800 μL de etanol absoluto.