In case of delays, the use of transport media such as Stuart medium is recommended. These samples should be stored at 4o to 8 °C for no longer than 24 hours before processing. Next, the sample should be homogenized and duplicates should be cultured in different kind of media. Culture media that can be used include Brucella broth and agar, BHI, Muller Hinton, Columbia agar and trypticase soy agar. These are generally supplemented with Fetal Bovine Serum, lysed erythrocytes, yeast extract, peptone and cyanobacteria. 7% to 10% horse, lamb or rabbit blood is generally added.
Si se prevé una demora, se deben utilizar medios de transporte como el medio Stuart y mantenerse a una temperatura de 4 a 8 °C, por no más de 24 horas antes del procesamiento, posteriormente se debe realizar un homogenizado y sembrar por duplicado en diferentes medios de cultivo entre ellos, caldo y agar Brucella, BHI, Mueller Hinton, agar Columbia y tripticasa de soja suplementados generalmente con suero fetal bovino, lisado de eritrocitos, extracto de levadura, peptona y extracto de cianobacterias.