A multiplexed PCR was applied to the spleen colonies to simultaneously identify the deleted ery operon and the G gene. In the case of the rabies protein, the following inner primers were used: Gli1, forward 5'-ACA-CAATCCGTACCCTGACT-3', and Gli2, reverse 5'-CCCGTTTACATGAGGATGAC-3'.
A las colonias del bazo se les aplicó una PCR múltiple para identificar simultáneamente el operón ery eliminado y el gen G. Para la proteína de la rabia se utilizaron los siguientes primers internos: Gli1, delantero 5'-ACACAATCCGTACCCT-GACT-3', y Gli2, reverso 5'-CCCGTTTACATGAGGATGAC-3'.