Two minutes were allowed to elapse so that subjects could adopt a comfortable position in the dental chair, and then assume a 90 degree position. Baseline saliva samples were harvested with the help of a saliva collector glass and funnel, so as to spit into the tube as indicated in the Tomas Self non-stimulated saliva collection method. After this, subjects were given a 30 gram Nestle Sublime chocolate tablet for consumption, and finally 10 minutes later, saliva was once more harvested so as to measure its pH.
Se les brindaron dos minutos para que se acomodaran en una silla con el propósito de adoptar una posición de 90 grados y se les tomó la muestra de saliva basal empleando un vaso colector de saliva y un embudo para escupir en el tubo, como indica el protocolo establecido del método de recolección para saliva no estimulada de Tomas Seif; después se les proporcionó una barra de chocolate Sublime de Nestlé de 30 gramos para su consumo y finalmente se volvió a recolectar la saliva a los 10 min para su respectiva medición de pH.