Starting with cultures which had been incubated for 2- to 3 days, suspensions were prepared in Brucella broth adjusted to 2 on the MacFarland scale 1 x 108 CFU / ml. The suspension was inoculated with a sterile swab on Mueller-Hinton agar plates and supplemented with 10% of horse serum and 2% of Isovitalex.
A partir de cultivos de 2 a 3 días de incubación, se preparó una suspensión en caldo Brucella que se ajustó a la escala 2 de MacFarland 1 x 10 UFC/ml, la suspensión se inoculó con un escobillón estéril sobre placas de agar Mueller-Hinton suplementado con 10% de suero de caballo, 2% de isovitalex.