To obtain a transgenic plant, the genetic construct to be inserted had to cross the cell wall, the plasma membrane and the nuclear membrane; of course, all this had to be done without destroying the plant cell, and a whole plant then had to be regenerated.
Para obtener una planta transgénica era necesario que la construcción genética a introducir atraviese la pared celular, la membrana plasmática y la membrana nuclear; todo eso, por supuesto, sin destrozar la célula vegetal, y regenerando luego una planta entera.