Samples were disinfected with commercial 3 % sodium hypochlorite for 5 minutes; they were rinsed three times in sterile distilled water for 5 min each; they were dried with sterile absorbent paper and three sections of each sample were sown in potato-dextrose agar 2 % PDA, Bioxon culture medium at 25 ± 2 °C considered as replications, which made a total of 42 samples which were maintained for 10 days.
Las muestras se desinfestaron con hipoclorito de sodio comercial 3 % por 5 minutos; se enjuagaron en tres ocasiones con agua destilada estéril por 5 min cada vez; se secaron con papel absorbente estéril y se sembraron tres secciones de cada muestra en medio de cultivo papa-dextrosa-agar 2 % PDA, Bioxon e incubaron a 25 ± 2 °C consideradas como repeticiones, lo que hizo un total de 42 muestras, que se mantuvieron rn rsas condiciones durante 10 días.