Serial dilutions of each passage were performed to determine the CFU/mL in triplicate under 3 conditions: 1 bacteria cultivated in TSB and isolated in TSA TSB/TSA; 2 bacteria cultivated in TSB and isolated in TSA-Amp TSB/TSA-Amp; and 3 control culture, consisting of bacteria cultivated in TSB-Amp and isolated in TSA-Amp TSB-Amp/TSA-Amp.
De cada pasaje se realizaron diluciones seriadas para determinar por triplicado la cantidad de UFC/mL bajo tres condiciones: 1 bacterias cultivadas en CST y aisladas en AST CST/AST; 2 bacterias cultivadas en CST y aisladas en AST-Amp CST/AST-Amp, y 3 cultivo testigo, constituido por bacterias cultivadas en CST-Amp y aisladas en AST-Amp CST-Amp/AST-Amp.