Once the genetic material had been obtained, specific primers flanking the 5UTR region were used to amplify a highly conserved sequence of HCV 7 with RT-PCR. For reverse transcription and the first round primer set ATACTCGAGGTGCACGGTCTACGAGACCT/nt299-nt327 and CTGTGAGGAA CTACTGTCTT/nt23-nt42 were used. For the second round primers CACTCTCGAGCACCCTATCAGGCAGT/nt266-nt292 group and TTCACGCAGAAAGCG TCTAG/nt41-nt60 were used.
Una vez obtenido el material genético, se amplificó por RT-PCR una secuencia altamente conservada para el HCV 7, mediante la utilización de cebadores que flanqueaban específicamente la región 5UTR: para transcripción reversa y primera ronda el set de primers ATACTCGAGGTGCACGGTCTACGAGACCT/nt299-nt327 y CTGTGAGGAA CTACTGTCTT/nt23-nt42, y para la segunda ronda el grupo de primers CACTCTCGAGCACCCTATCAGGCAGT/nt266-nt292 y TTCACGCAGAAAGCGTCTAG/nt41-nt60.