The vaginal samples were taken using sterile swabs and were transported in tubes with 2 ml of sucrose-phosphate/glutamate medium SPG 217 mM sucrose, 4 mM KH2PO4, 7 mM K2HPO4, and 1% L-Glutamine, supplemented with 10% fetal bovine serum FBS and antibiotics 100 µg/ml streptomycin, 50 µg/ml gentamicin Sachse et al., 2009.
Las muestras vaginales se tomaron con hisopos estériles y se transportaron en tubos con 2 ml de medio de sacarosa-fosfato/glutamato SPG sacarosa 217 mM, KH2PO4 4 mM, KH2PO4 7 mM y L-glutamina 1% suplementados con 10% de suero fetal bovino SFB y antibióticos 100 µg/ml estreptomicina, 50 µg/ml gentamicina Sachse et al., 2009.