PCR products were purified using Wizard SV Gel Kit and PCR Clean-Up System Promega, Madison-Wisconsin, USA, following manufacturers instructions. A 25 μL final volume elution was performed.
Los productos de la PCR fueron purificados utilizando el kit Wizard SV Gel and PCR Clean-Up System Promega, Madison-Wisconsin, USA, siguiendo las recomendaciones del fabricante, realizando la elusión en un volumen final de 25 µL.