The concentration of spores was estimated using a Neubauer chamber Neubauer Improved bright-line, Marienfield, Germany and bacteria were quantified by technique of serial dilutions and plate colony count.
La concentración de esporas se estimó usando una cámara de Neubauer Neubauer Improved bright-line, Marienfield, Alemania y las bacterias se cuantificaron por la técnica de diluciones seriadas y conteo de colonias en placa.