Their spleens were weighed and homogenized in 1 mL of PBS and plated on either TSA or TSA-Amp, and the CFU/spleen value was determined by serial dilution.
Los bazos se pesaron y homogenizaron en 1 mL de PBS, se sembraron en placas de AST y AST-Amp y se determinó la cantidad de UFC/bazo mediante diluciones seriadas.