Samples were withdrawn after 60 minutes, and then were individually submerged in 1 mL deionized water in a plastic container, and stored in a chamber at 37 ±1 C Felisa. 24 hours later, samples were withdrawn from the storing solution, and rinsed with 1 mL deionized water.
Las muestras fueron retiradas de los hacedores 60 minutos después, e inmersas individualmente en 1 mL de agua desionizada en un recipiente de plástico, y se almacenaron en una cámara a 37 ± 1 C Felisa.