600 mesh carborundum was sprinkled over a surface of approximately 5 cm on the upper portion of the cladode; the inoculum was taken with a cotton swab and was rubbed over the surface.
Se espolvoreó carborundum de 600 mallas sobre una superficie aproximada de 5 cm en la porción superior del cladodio, y con un hisopo se tomó el inóculo y se frotó sobre dicha superficie.