To generate each cDNA template, RT was achieved, osteocalcin specific oligo in sense - direction 5' GATCCATGAGAATGAGAAGCG 3'and direction-anti-sense 5'CTATGTTAGCACCTTATCCCC 3' at 45°C during 30minutes, and a 94°C cycle for two minutes. cDNA was amplified under the following PCR program: 94°C during one minute de-naturalization, 55°C alignment temperature for the osteocalcin oligos during one minute, and extension to 72°C for one minute.
Para generar cada templado de cDNA el RT se llevó a cabo usando los oligos específicos para la osteocalcina: oligo en dirección sentido 5' GATCCATGAGAATGAGAAGCG 3' y oligo en dirección antisentido 5' CTATGTTAGCACCTTATCCCC 3' a una temperatura de 45°C por 30 minutos y un ciclo de 94°C por 2 minutos.