A second blood sample was taken from all patients who had been tested for anti-HCV reactivity. To confirm the presence of the virus, these samples were tested for HCV RNA with qualitative real time polymerase chain reaction RT-PCR using AMPLICOR HCV MONITOR Test Version 2.0 from Roche Molecular Systems.
A todos los pacientes que presentaron la prueba de anti-HCV reactiva, se les tomó una segunda muestra de sangre periférica para la búsqueda del RNA del virus de la hepatitis C y así confirmar la infección por este virus: RT-PCR cualitativa-Amplicor HCV Monitor 2.0 Roche Molecular Systems.