Police Battalion 320 was formed in 1939 as part of the major process during which 38 police brigades were formed from 26,000 young volunteers. Police Battalion 320 was trained in the vicinity of Berlin. Afterwards, it served in the Protectorate of Bohemia and Moravia. In July 1941 it was included in the five police battalions that were subordinated to Jeckeln, the Higher SS and Police Leader of the South in the USSR, with the aim of facilitating the repression and murders that were being carried out under Jeckeln’s command. In this context members of the Battalion surrounded the site where tens of thousands of Jews from Kamenets-Podolsk were murdered between the 26th and 28th of August 1941 and they murdered 2,2000 Jews in Minkovtsy on the 30th or 31st of August of the same year. On the following day members of the Battalion shot 380 Jews in Zhvanchik Velikiy (Zwianczyk) and Sokolets (Sokolec), both located about 30 kilometers northeast of Kamenets-Podolsk.

In July 1942 the Battalion was transformed into Battalion III of SS Police Regiment 11.