Drugs of Abuse pp 193-209 | Cite as

Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction

  • Joshua D. Berke
Part of the Methods In Molecular Medicine™ book series (MIMM, volume 79)


Differential display polymerase chain reaction (DDPCR) is a technique that allows comparisons between the expressed mRNA population in two or more tissues, or in the same tissue under two or more different conditions. For example, it has been used to discover striatal genes whose expression is altered following a drug injection (1,2). The essential idea behind DDPCR is to generate an RNA “fingerprint,” a pattern of bands corresponding to the expressed mRNA population. By performing parallel sets of reactions for each experimental condition and displaying the resulting fingerprints side by side, bands that differ between conditions can be noted and the corresponding mRNA identified.


Vortex Dopamine Adenosine Electrophoresis Syringe 


  1. 1.
    Douglass, J., McKinzie, A. A., and Couceyro, P. (1995) PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamineai. J. Neurosci. 15, 2471–2481.PubMedGoogle Scholar
  2. 2.
    Berke, J. D., Paletzki, R. F., Aronson, G. J., Hyman, S. E., and Gerfen, C. R. (1998) A complex program of striatal gene expression induced by dopaminergic stimulation. J. Neurosci. 18, 5301–5310.PubMedGoogle Scholar
  3. 3.
    Berke, J. D., Sgambato, V., Zhu, P-P., et al. (2001) Dopamine and glutamate induce distinct striatal splice forms of Ania-6, an RNA polymerase II-associated cyclin. Neuron 32, 277–287.PubMedCrossRefGoogle Scholar
  4. 4.
    Liang, P. and Pardee A. B. (1992) Differential display of eukaryotic messenger RNA by polymerase chain reaction. Science 257, 967–971.PubMedCrossRefGoogle Scholar
  5. 5.
    Liang, P., Zhu, W., Zhang, X., et al. (1994) Differential display using one-base anchored oligo-dT primers. Nucleic Acids Res. 22, 5763–5764.PubMedCrossRefGoogle Scholar
  6. 6.
    Schaefer, B. C. (1995) Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends. Analyt. Biochem. 227, 255–273.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Joshua D. Berke
    • 1
  1. 1.Laboratory of Cognitive Neurobiology, Department of PsychologyBoston UniversityBoston

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