MSG is produced through fermentation of molasses-sugar (molasses) by the bacteria (Brevibacterium lactofermentum). In this fermentation process, will first produce Glutamic Acid. Glutamic acid as a result of this fermentation process, then add soda (Sodium Carbonate), so that will be formed Monosodium Glutamate (MSG). MSG this happens, then purified and crystallized, so it is a crystal-pure powders, ready to be sold in the market.

Before the bacteria were used for fermentation production of MSG, the first such bacteria must be reproduced (in terms of microbiology: cultured or cultured) in a medium called Bactosoytone. This process is known as the breeding process of bacteria, and separate at all (both space and time) with the process above. After the bacteria grow and multiply, then the bacteria were taken for use as biological agents in the fermentation process to make MSG.

Bactosoytone as a medium for bacterial growth, made separate (by Difco Company in the U.S.), by the way-enzymatic hydrolysis of soy protein (Soyprotein). In simple language, soy protein is broken down with the help of enzymes that produce short-chain peptides (peptone) Bactosoytone called it. The enzyme used in hydrolysis process is called porcine, and this enzyme was isolated from the pancreas-pig.

It is worth highlighting that, porcine enzyme used in the manufacturing process Bactosoytone media, only serves as a catalyst, meaning it only affects the enzyme hydrolysis reaction rate of soy protein into Bactosoytone, WITHOUT go in the molecular structure Bactosoytone it. So Bactosoytone produced from-enzymatic hydrolysis process, the CLEAR-FREE from the elements pig!, Other than because it happens Bactosoytone products undergo a process of "clarification" before being used as growth media, also because it is the element of porcine enzyme is not included in Bactosoytone molecular structure, because only as a catalyst only porcine
Clarification process in question is the separation of the porcine enzyme Bactosoytone happened. This process is done by heating 160oF for at least 5 hours, then made filtration, to separate the enzyme from porcine Bactosoytone his product. Filtrate that is clean is then evaporated, and taken Bactosoytone happens.

It is worth highlighting that the process of making this Bactosoytone Media is an entirely separate process with the process of making MSG. Media Bactosoytone is a bacterial growth media, and sold in the market, not just for bacteria MSG maker, but also for other bacteria are used for making other industrial-biotech products.

Note: Bactosoytone name is a trade name, which can be parsed as follows: Bacto is a trade name of the factory is the manufacturer (Difco Co.); Soy of origin of the word soybean: soybean, tone, abbreviation of Peptone; so Bactosoyton means soy peptone made by the factory Difco .
Once the bacteria are grown on media bactosoytone, then moved to the Media Liquid Starter. This media did not contain bactosoytone. In the Media Liquid Starter these bacteria multiply and grow rapidly.

Then, this breed of bacteria that have entered into Liquid Media Productions, where the bacteria begin to produce glutamic acid, which is then converted into MSG. Production of Liquid media also does not contain bactosoytone.

It should be mentioned here that MSG-producing bacteria Brevibacterium lactofermentum or Corynebacterium is glutamicum, are bacteria that live and thrive in water media. So, the bacteria that includes the aqueous microorganisms.

Results of research conducted by the Directorate General of POM in Jakarta showed that:
Bactosoytone not contaminated (not mixed) with fat pork (Data Analysis of Gas Chromatography); Protein pigs (HPLC analysis of data), and pig DNA (PCR analysis of data.) MSG does not contaminated (not mixed) by: Fat pigs (Data Analysis of Gas Chromatography); Protein pigs (HPLC analysis of data), and pig DNA (PCR analysis of data.)

Result Analysis conducted in Japan (Kyoto University) also showed that both MSG and Bactosoytone not contaminated by pork enzymes.