Molecular Biology Virtual Lab I


Preparation of Buffer stocks (TBE,TE and TAE)
Buffers are very important for most of the biological reactions. Its preparation is the prior step in laboratory experiments.
Plasmid Isolation (Mini prep)
Plasmid is a double stranded, circular extra chromosomal DNA of bacterium. It is used in recombinant DNA experiments to clone genes from other organisms and make large quantities of their DNA.
Extraction of DNA from Fish Fins
From this experiment you will be able to isolate / extract DNA from Eukaryotes and Prokaryotes.
Hot Shot Method of DNA Extraction
DNA isolation is necessary for genetic analysis, which is used for scientific, medical, or forensic purposes.Hot Shot method is found to be rapid, reliable, and inexpensive for the isolation of PCR-quality DNA than the traditional methods
Agarose Gel Electrophoresis (AGE)
Agarose gel electrophoresis is the easiest and commonest way of separating and analyzing DNA. Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through an agarose matrix.
Restriction Digestion
To understand the method of digesting DNA with different restriction enzymes.
Maintenance and Storage of DH5alpha E.coli cells
Effective storage means that the organism is being maintained in a viable state free of contamination and without changes in genotypic or phenotypic characteristics.
Preparation of Competent Cell (Calcium Chloride Treatment)
To familiarize with how cells are made competent which is the primary step for transformation.
Transformation of the Host Cells
The purpose of transformation is to introduce a foreign plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities of it.
Extraction of DNA from Agarose gel
DNA extraction is an important step in molecular biology techniques in order to obtain specific DNA molecules from the cells.

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