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A Little Space, Please

SciVis and InfoVis face off

Robert Kosara

Every branch of science needs to observe phenomena and each has its instruments: microscopes, telescopes, spectrometers, interferometers. While many phenomena have meaningful, intrinsic spatial components, many more do not: the spectral composition of light, the genetic variety in a habitat or observations in a clinical study. To fully observe these, we need not only instruments to measure data, but also a tool that helps makes sense of it all: visualization.



Combination%20of%20scientific%20and%20information%20visualization%20of%20a%20man's%20headClick to Enlarge ImageThe image at right combines both approaches. At the top, there is a three-dimensional, SciVis rendering of CT (computed tomography) measurements of a man’s head. Below that, data measured within the head—curvature of structures, the boundaries between them and more—are isolated from each other in space. Separate and mostly triangular fields of varying densities and colors allow a trained viewer to better understand structures of interest within the head.

Bioinformatics, which uses computers and mathematics to make sense of biology, also greatly benefits from visualization. I was reminded of this recently by Anthony Fodor, a colleague at the University of North Carolina, Charlotte. Fodor works in metagenomics, the study of genetic material retrieved from environmental samples. In recent research he used common but effective visualizations to explore bacterial genomic data collected in a wastewater treatment plant.

Visualized%20bioinformatics%20comparing%20DNA%20segmentsClick to Enlarge ImageHis team used pyrosequencing to extract short segments of DNA (around 250 base pairs) from individual organisms in a sample. The resulting data set of about 350,000 sequences was compared to known bacteria genomes using the widely accepted BLAST algorithm. As it turned out, more than 60 percent of the DNA sequences retrieved from such a well-controlled, artificial environment did not match known bacterial sequences. Even partial sequence matches were not common. The best match, the known bacteria Acidovorax JS42, shared only about 25 percent of its sequence with DNA retrieved from the plant. To better examine these strangers, Fodor isolated plasmids, even shorter DNA segments that bacteria exchange during reproduction, from his sample. Potentially they could produce more matches with known bacteria. As the upper right figure shows, they produced fewer.

To take a look at the treatment-plant organisms from a different vantage point, Fodor and colleagues developed an interactive program that let them scroll through the entire genome of a particular organism. The lower screenshot at right shows DNA segment sequences shared by the treatment plant bacteria and, again, Acidovorax JS42. The base pair position of the known bacteria’s sequence is denoted on the horizontal axis; the vertical axis represents how frequently matching DNA from plant samples matched specific sequence spans. Black rectangles represent data from the treatment pools; red rectangles show data from a seawater sample for comparison.

Even though the vast majority of the DNA segments are not shared across the two samples, here one can easily spot frequently shared code. In Acidovorax JS42, this code is positioned in non-gene, or non-coding, parts of its genome, territory once considered “junk DNA” that hosts a transposon. Transposons are short DNA segments once known as jumping genes that can cut and paste themselves in a cell’s DNA. Transposons can activate non-coding DNA, and evidence is growing that, unlike most genomic regions, they are widely conserved in different environments. Based on this and other evidence, Fodor has hypothesized that “perhaps all life only exists to shepherd transposons.”

While these visualizations are simple, they are powerful. Without them, important patterns would be almost impossible to discover. For nonspatial data such as these, visualization is the microscope.

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