denaturation temperature of DNAmedical dictionary

That temperature at which, under a given set of conditions, double-stranded DNA is changed (50%) to single-stranded DNA; under standard conditions, the base composition of the DNA can be estimated from the denaturation temperature, since the greater the denaturation temperature, the greater the guanine-plus-cytosine content (i.e., GC content) of the DNA.

Synonyms: melting temperature of DNA.

(05 Mar 2000)

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