Forming secretory tubules in liver

23 May 2016. NUS scientists have discovered a unique demonstration of how biomimetic interfaces can be used to probe and understand the influence of the microenvironment on the cellular process.

A team of scientists led by Prof Virgile VIASNOFF from the Department of Biological Sciences in NUS and the French National Centre for Scientific Research (Centre national de la recherche scientifique, or CNRS), the largest Governmental research organisation in France, and Prof Hanry YU, at Mechanobiology Institute and at the Institute of Bioengineering and Nanotechnology, of A*STAR, has revealed the underlying mechanism for the formation and growth of a fundamental type of tissue – epithelial tubes. Defects in the architecture of epithelial tubes lead to diseases such as cholestasis, atherosclerosis and polycystic kidney disease. The research findings contribute towards a deeper understanding of the principles that underlie epithelial tube formation, and offer opportunities for developing better therapies for such diseases.

The study suggests that the shape and size of some types of epithelial tubes are governed by the mechanical forces that arise from the interaction of cells with the supportive extracellular matrix (ECM) that surrounds them. The work and its findings were published in the scientific journal Nature Cell Biology in March 2016.

All the major organs in the human body, such as the blood vessels, lungs, kidneys, liver, pancreas and the intestine, comprise an extensive network of tubes. These tubes function as biological pipelines that transport and deliver life-sustaining liquids, gases or macromolecules from one site in the body to another. Depending on the organ in which they are formed or the specific function that they perform, the tubes vary greatly in size and shape. Defects in their tubular architecture have been linked to a number of diseases, such as atherosclerosis and polycystic kidney disease.

The tubes enclose hollow spaces called lumens and are primarily composed of a single or multiple layers of epithelial cells. As an important prerequisite for tube formation, epithelial cells become asymmetric or ‘polar’, acquiring structurally and functionally distinct ends or surfaces. Following this, cells undergo shape changes and organise around a central lumen, with their apical (top) surfaces facing the lumen, the basal (bottom) surfaces interacting with the underlying tissue and the lateral (side) surfaces in close contact with the neighbouring cells. The vast majority of studies on tube formation have focused on understanding the molecular mechanisms leading to cell polarisation and subsequent cellular mechanisms that drive the formation of lumens. However, factors that regulate the shape, size and the directional elongation of lumens into tubes remain unclear.

Studying the formation of ‘bile caniculi’, which are lumens formed between the contacting lateral surfaces of two liver cells, the scientists adopted a ‘minimal organ approach’. This involved culturing two liver cells (hepatocytes) that can act as a functional organ unit on artificial membranes fabricated with microwell patterns. The microwells are coated with an ECM protein called fibronectin that promotes cell binding and creates growth conditions identical to the microenvironment found inside cells. By coating the microwells in different patterns, the scientists altered the organisation of ECM around cells and compared the morphologies of the bile caniculi and the direction of their growth. Surprisingly, they observed that lumen shape was controlled by the three-dimensional organisation of ECM around cells. Furthermore, lumens showed a preference to elongate towards the free surface of the cell, away from the ECM.

Following up with a series of experiments to understand the role of ECM in determining lumen shape and elongation, the researchers proposed a mechanical basis for the regulation of lumen morphology. According to their model, forces arising from the adhesion of cells to the ECM influence the force balance inside cells and create an intercellular force (force between two contacting cells) gradient. The lumen elongates along the direction of minimal force, as higher intercellular force would squeeze the contacting cell surfaces together and prevent extension of the lumens in that direction.

This study reveals for the first time, that the interaction between cells and the ECM can control and direct the mechanical tension between cells. This mechanical tension directly influences the elongation direction of the intercellular lumen. This mechanical guidance of lumen morphology is responsible for differences in lumen shapes and sizes, formed under different microenvironmental conditions.

Prof Viasnoff concluded that this approach offers a very promising way to understand not only tube formation but also cell polarisation. More broadly, the team expects this study to be a first step towards understanding how the environment surrounding cells affects their interactions in normal and diseased cases.


Figure shows different morphologies of bile canaliculi can be achieved by controlling the environment around the cells [Image credit: Virgile Viasnoff]



Li Q, Y Zhang, P Pluchon, J Robens, K Herr, M Mercade, JP Thiery, H Yu, V Viasnoff. "Extracellular Matrix Scaffolding Guides Lumen Elongation by Inducing Anisotropic Intercellular Mechanical Tension." Nat Cell Biol 18, 3 (2016) 311.