Humans, sharks share immune-system feature

Sep 30, 2011



The T cell serves as the sentinel, manager and enforcer of the adaptive immune response. It relies on its receptor, the T-cell receptor, to recognize foreign material and identify the target of the immune-system attack. When the receptor binds to small fragments of foreign organisms, called antigens, the T cell becomes activated, proliferates and initiates an attack against any molecule or organism containing that antigen.

T cells, however, cannot recognize free-floating antigens. They recognize antigens only when they are held by MHC on the surfaces of other cells, much as a hotdog bun (MHC molecule) holds a hotdog (antigen). This interaction between the T cell and MHC molecules is crucial for immune defense and organ transplants. Compatibility of transplanted organs is determined by the similarity of different people’s MHC molecules. Nonetheless, this interaction has long mystified scientists and is poorly understood.

In 1971 future Nobel Laureate Niels K. Jerne proposed that evolution might have selected for genes that specifically recognize MHC molecules. Evidence discovered later suggested T cells’ affinity for MHC molecules might instead be the product of development that occurs as T cells mature in the thymus. The question remained unanswered for 40 years.

The T-cell receptor is constructed by piecing together several peptides among dozens that are available, plus a few random amino acid sequences. This combination is what allows the to generate an almost infinite variety of receptors capable of recognizing almost any potential invader. The receptor has six loops that are the primary binding points for the antigen-MHC complex. One of those loops, known as CDR2, frequently binds the MHC molecule.

Searching for possible similarities in T-cell receptors of different animals, the researchers compared the amino acid sequences of one segment of the T-cell receptor containing the CDR2 loop. Although the segments contained less than 30 percent of the same , two specific amino acids were the same in human, mouse, frog, trout and shark T-cell receptors. Those appeared to be amino acids specifically involved in binding to the MHC molecule.

“The evolutionary inheritance of this pattern goes all the way from sharks to humans, which last shared a common ancestor 450 million years ago,” said co-author Philippa Marrack, PhD.

The researchers then inserted segments containing the CDR2 loop from frog, trout and shark T-cell receptors into mouse cells. These chimeric T-cell receptors recognized antigen bound to a mouse MHC molecule.

Since sections of , and shark T-cell receptors functioned perfectly well in T-cell , the experiments suggested that the T-cell’s ability to see an antigen only when complexed with an MHC molecule first arose more than 400 million years ago, when all four animals shared a .


Provided by National Jewish Health

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